BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC143341.7 + phase: 0
(745 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9160 similar to UP|Q8H0V0 (Q8H0V0) Isp4 like protein, partial ... 425 e-119
CB826638 246 1e-65
AV769817 194 3e-50
BP064592 130 8e-31
AU089292 123 1e-28
TC11382 similar to PIR|T01900|T01900 isp4 protein homolog T12H20... 103 1e-22
AV418884 92 4e-19
BP075713 43 2e-04
TC13937 weakly similar to UP|Q9LVE0 (Q9LVE0) Nitrate transporter... 32 0.37
AW719889 29 2.4
AV768992 29 2.4
TC19957 28 4.1
TC18780 weakly similar to UP|Q9LMD4 (Q9LMD4) F14D16.8, partial (... 28 5.3
TC16792 28 5.3
TC17855 28 7.0
AV779579 27 9.1
BP057615 27 9.1
>TC9160 similar to UP|Q8H0V0 (Q8H0V0) Isp4 like protein, partial (53%)
Length = 1554
Score = 425 bits (1093), Expect = e-119
Identities = 194/401 (48%), Positives = 274/401 (67%)
Frame = +2
Query: 344 TETFDIDMESYSNYSKIYLSVAFAFEYGFCFAALTATISHVVLFHGEMIVQMWKKTTTSL 403
T +++++++Y+ Y K+YLS FA G FA TAT++HV LFHG I++ +
Sbjct: 11 TPDYELNVDAYNQYGKLYLSPLFALSIGSGFARFTATLTHVALFHGSDILRQSRSAMNKA 190
Query: 404 KNQLGDVHTRIMKKNYEQVPEWWFVTILILMVMMALLACEGFGKQLQLPWWGILLSLTIA 463
K DVH R+MK Y+QVPEWWF+ +L + ++L+ + + +QLPWWG+L + +A
Sbjct: 191 KL---DVHGRLMKA-YKQVPEWWFLILLFGSMALSLVMSFVWKRDVQLPWWGMLFAFALA 358
Query: 464 LIFTLPIGVIEATTNIRSGLNVITELVIGFIYPGKPLANVAFKTYGHISMVQALGFLGDF 523
I TLPIGVI+ATTN + G ++I + +IG++ PGKP+AN+ FK YG IS V AL FL D
Sbjct: 359 FIVTLPIGVIQATTNQQPGYDIIAQFMIGYVLPGKPIANLLFKIYGRISTVHALSFLSDL 538
Query: 524 KLGHYMKIAPKSMFIVQLVGTVVASSVHFGTAWWLLTSIENICDESLLPKGSPWTCPGDD 583
KLGHYMKI P+ M+ QLVGT+VAS V+ AWW+L +I++IC + +PWTCP
Sbjct: 539 KLGHYMKIPPRCMYTAQLVGTLVASIVNLAVAWWMLDNIKDICMDDKAHHDNPWTCPKFR 718
Query: 584 VFYNASIIWGVVGPKRMFTKDGVYPELNWFFLIGLIAPVPVWLLSLKFPNQKWIQFINIP 643
V ++AS+IWG++GP+R+F G+Y L W FLIG + PVPVW+LS FP +KWI INIP
Sbjct: 719 VTFDASVIWGLIGPRRLFGPGGLYRNLVWLFLIGAVLPVPVWVLSKIFPEKKWIALINIP 898
Query: 644 IIIAGASDIPPVRSVNYITWGIVGIFFNFYVYRKFKAWWARHTYILSAGLDAGVAFIGLL 703
+I G + +PP N +W + G+ FNF+V+R + WW ++ Y+LSA LDAG AF+G+L
Sbjct: 899 VISYGFAGMPPATPSNIASWLVTGMIFNFFVFRYHQRWWQKYNYVLSAALDAGTAFMGVL 1078
Query: 704 LYFSLQSYGIYGPTWWGLEPDHCPLAKCPTAPGVHAEGCPV 744
++F+L + G + WWG E D CPLA CPTAPG+ +GCPV
Sbjct: 1079IFFALPNAG-HSLKWWGSEMDPCPLASCPTAPGIKVDGCPV 1198
>CB826638
Length = 575
Score = 246 bits (627), Expect = 1e-65
Identities = 119/142 (83%), Positives = 132/142 (92%)
Frame = +1
Query: 2 SSSRVSQNTVIEDAEIDEYSINDSPIEQVRLTVPITDDPSQPALTFRTWVLGLASCILLA 61
SS VSQN EDA+ D+Y++ND PIEQVRLTVPITDDP+QPALTFRTWVLGLASC+LLA
Sbjct: 148 SSRGVSQNRATEDADKDQYTVNDCPIEQVRLTVPITDDPTQPALTFRTWVLGLASCVLLA 327
Query: 62 FVNQFLGYRTNPLKITSVSAQIIALPLGKLMAATLPTKPIQVPFTTWSFSLNPGPFSLKE 121
FVNQFLGYRTNP+ ITSVSAQI+ LPLGKLMAATLPT+PI+VPFT+WSFSLNPGPFS+KE
Sbjct: 328 FVNQFLGYRTNPMNITSVSAQIVTLPLGKLMAATLPTRPIRVPFTSWSFSLNPGPFSMKE 507
Query: 122 HVLITIFASSGSSGVYAINIIT 143
HVLITIFASSGS GVYAI+IIT
Sbjct: 508 HVLITIFASSGSGGVYAISIIT 573
>AV769817
Length = 436
Score = 194 bits (494), Expect = 3e-50
Identities = 83/145 (57%), Positives = 110/145 (75%)
Frame = +2
Query: 420 EQVPEWWFVTILILMVMMALLACEGFGKQLQLPWWGILLSLTIALIFTLPIGVIEATTNI 479
E +P WWF +L+ + ++L+ C Q+Q+PWWG+L + +A +FTLPI +I ATTN
Sbjct: 2 EDIPSWWFYALLVATLAISLVLCIFLNDQIQMPWWGLLFAGALAFVFTLPISIITATTNQ 181
Query: 480 RSGLNVITELVIGFIYPGKPLANVAFKTYGHISMVQALGFLGDFKLGHYMKIAPKSMFIV 539
GLN+ITE V G IYPG+P+ANV FKTYG+ISM QA+ FL DFKLGHYMKI P+SMF+V
Sbjct: 182 TPGLNIITEYVFGLIYPGRPIANVCFKTYGYISMAQAVSFLSDFKLGHYMKIPPRSMFLV 361
Query: 540 QLVGTVVASSVHFGTAWWLLTSIEN 564
Q +GTV+A +++ G AWWLL SI+N
Sbjct: 362 QFIGTVLAGTINIGVAWWLLNSIDN 436
>BP064592
Length = 531
Score = 130 bits (327), Expect = 8e-31
Identities = 54/72 (75%), Positives = 62/72 (86%)
Frame = -2
Query: 672 FYVYRKFKAWWARHTYILSAGLDAGVAFIGLLLYFSLQSYGIYGPTWWGLEPDHCPLAKC 731
FYVYR FK WW TYILSA LDAGVAF+GLLLYF+L S G++GPTWWGL+ DHCPLA+C
Sbjct: 530 FYVYRNFKXWWXSXTYILSAALDAGVAFMGLLLYFALPSNGVFGPTWWGLDNDHCPLARC 351
Query: 732 PTAPGVHAEGCP 743
PT PGV+A+GCP
Sbjct: 350 PTYPGVYAKGCP 315
>AU089292
Length = 345
Score = 123 bits (308), Expect = 1e-28
Identities = 51/106 (48%), Positives = 77/106 (72%)
Frame = +2
Query: 561 SIENICDESLLPKGSPWTCPGDDVFYNASIIWGVVGPKRMFTKDGVYPELNWFFLIGLIA 620
+I ++CD S LP PW CP D+VFY+AS+IWG++GP+R+F GVY ++N+FFL G IA
Sbjct: 11 TIPDLCDASKLPTDXPWLCPMDNVFYDASVIWGLLGPRRIFGDQGVYGKVNYFFLGGAIA 190
Query: 621 PVPVWLLSLKFPNQKWIQFINIPIIIAGASDIPPVRSVNYITWGIV 666
P+ VWL FP ++W++ I++PI++ S +PP +VNY +W +V
Sbjct: 191 PLLVWLAHKAFPXKEWLRLIHMPIMLGATSMMPPATAVNYTSWLLV 328
>TC11382 similar to PIR|T01900|T01900 isp4 protein homolog T12H20.7 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(10%)
Length = 606
Score = 103 bits (256), Expect = 1e-22
Identities = 52/97 (53%), Positives = 66/97 (67%), Gaps = 1/97 (1%)
Frame = +3
Query: 1 MSSSRVSQNTVIEDAEIDEYSIND-SPIEQVRLTVPITDDPSQPALTFRTWVLGLASCIL 59
M S+ ++ E E +E I + SP++QV LTVP TDDPS LTFR W LG+ SC+L
Sbjct: 315 MEGSKTTEEVNREHEEEEEEEIEEESPVKQVELTVPKTDDPSLHILTFRMWFLGVVSCVL 494
Query: 60 LAFVNQFLGYRTNPLKITSVSAQIIALPLGKLMAATL 96
L+FVNQF YRT PL +TS+SAQI +P+G MA TL
Sbjct: 495 LSFVNQFFWYRTEPLTVTSISAQIAVVPIGHFMARTL 605
>AV418884
Length = 418
Score = 91.7 bits (226), Expect = 4e-19
Identities = 46/86 (53%), Positives = 57/86 (65%)
Frame = +2
Query: 1 MSSSRVSQNTVIEDAEIDEYSINDSPIEQVRLTVPITDDPSQPALTFRTWVLGLASCILL 60
M+S Q T D +IDE + SP+E+VRLTV TDDP+QP TFR W LGL SC LL
Sbjct: 53 MNSDTEKQTTAATDVDIDEDEL--SPVEEVRLTVTNTDDPTQPVWTFRMWFLGLLSCSLL 226
Query: 61 AFVNQFLGYRTNPLKITSVSAQIIAL 86
+F+NQF YRT PL IT ++ Q+ L
Sbjct: 227 SFLNQFFAYRTEPLIITLITVQVSTL 304
>BP075713
Length = 381
Score = 43.1 bits (100), Expect = 2e-04
Identities = 16/27 (59%), Positives = 17/27 (62%)
Frame = -2
Query: 718 WWGLEPDHCPLAKCPTAPGVHAEGCPV 744
WWG E D CPLA CP PG +GC V
Sbjct: 245 WWGSEMDPCPLASCPLPPGSKVDGCHV 165
Score = 36.6 bits (83), Expect = 0.015
Identities = 24/64 (37%), Positives = 30/64 (46%), Gaps = 9/64 (14%)
Frame = -3
Query: 673 YVYRKFKAWWARHTYILSAGLDAGVAFIGLLLYF------SLQSYGIYGPTWWGLEP--- 723
+V+R WW ++ Y+LSA LDAG A G + L S G G W L P
Sbjct: 379 FVFRYPHPWWQKYNYVLSAALDAGPALHGCSNFLCPAHARPLVSNG--GDLKWTLAPWLL 206
Query: 724 DHCP 727
HCP
Sbjct: 205 AHCP 194
>TC13937 weakly similar to UP|Q9LVE0 (Q9LVE0) Nitrate transporter
(AT3g21670/MIL23_23), partial (27%)
Length = 558
Score = 32.0 bits (71), Expect = 0.37
Identities = 19/51 (37%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = +2
Query: 483 LNVITELVIGFIYPGKPLANVAFKTYGHISMVQALG-FLGDFKLGHYMKIA 532
+N++T LV P A + G +++V LG FL D KLG Y+ IA
Sbjct: 194 MNIVTYLVGVLNLPTAYSATIVTNCLGTMNLVSLLGGFLADVKLGRYLTIA 346
>AW719889
Length = 623
Score = 29.3 bits (64), Expect = 2.4
Identities = 14/29 (48%), Positives = 20/29 (68%), Gaps = 3/29 (10%)
Frame = +2
Query: 279 GFLGSPLAVPGFAI---INIMAGFFLYMY 304
GF+GS L + GFAI ++ GFFL++Y
Sbjct: 164 GFVGSFLGIFGFAIGIPFGLLLGFFLFVY 250
>AV768992
Length = 473
Score = 29.3 bits (64), Expect = 2.4
Identities = 12/18 (66%), Positives = 14/18 (77%), Gaps = 1/18 (5%)
Frame = -1
Query: 678 FKAWWARHTYI-LSAGLD 694
F AWWARH +I +S GLD
Sbjct: 395 FSAWWARHPWISISVGLD 342
>TC19957
Length = 642
Score = 28.5 bits (62), Expect = 4.1
Identities = 14/41 (34%), Positives = 21/41 (51%)
Frame = +1
Query: 196 SLFRAFHEKEKRPRGGTSRLQFFFVVFVASFAYYIIPGYFF 236
SL+ + + K P GG++ FF + S A PG+FF
Sbjct: 100 SLYEQYVGERK*PLGGSNCRLFFLATTLVSGASLAFPGFFF 222
>TC18780 weakly similar to UP|Q9LMD4 (Q9LMD4) F14D16.8, partial (18%)
Length = 393
Score = 28.1 bits (61), Expect = 5.3
Identities = 16/51 (31%), Positives = 27/51 (52%), Gaps = 4/51 (7%)
Frame = +3
Query: 665 IVGIFFNFYVYRKFKAWWARHTYILSAGLDAGVAF----IGLLLYFSLQSY 711
+VG ++ + + + H ++LS + AGVAF +GL LYF Q +
Sbjct: 156 VVGYIYDREAIEEDNSCFGIHCFMLSFFILAGVAFLSFLVGLALYFRTQKF 308
>TC16792
Length = 558
Score = 28.1 bits (61), Expect = 5.3
Identities = 20/76 (26%), Positives = 33/76 (43%)
Frame = +1
Query: 332 TGATYNVTRILNTETFDIDMESYSNYSKIYLSVAFAFEYGFCFAALTATISHVVLFHGEM 391
TG+TY+ ++ T + ++Y Y+ L +A A TA + VVLF
Sbjct: 22 TGSTYDPDELIETSVEKLGDQTYYKYA---LETPYALTGSHNLAKATAKGNTVVLFVASA 192
Query: 392 IVQMWKKTTTSLKNQL 407
Q W + ++K L
Sbjct: 193 NDQQWPTSQ*TVKAML 240
>TC17855
Length = 543
Score = 27.7 bits (60), Expect = 7.0
Identities = 11/32 (34%), Positives = 17/32 (52%)
Frame = +1
Query: 713 IYGPTWWGLEPDHCPLAKCPTAPGVHAEGCPV 744
++ ++W L DHC + C G AEG P+
Sbjct: 247 LFSISYWSLFEDHCCIKGCSRFWGCDAEGQPM 342
>AV779579
Length = 523
Score = 27.3 bits (59), Expect = 9.1
Identities = 20/52 (38%), Positives = 27/52 (51%)
Frame = +2
Query: 244 FVCLIWKESITAQQIGSGMKGLGIGSFGLDWNTVAGFLGSPLAVPGFAIINI 295
F C++W ESI GS LG + G+ T+A L S VP F I+N+
Sbjct: 284 FTCILWNESIFWLS-GSEFVSLGFSTSGI--TTLATPL-SMAQVPEFVILNL 427
>BP057615
Length = 588
Score = 27.3 bits (59), Expect = 9.1
Identities = 12/25 (48%), Positives = 14/25 (56%), Gaps = 3/25 (12%)
Frame = -1
Query: 710 SYGIYGPTWW---GLEPDHCPLAKC 731
S+G +GPTWW G E C A C
Sbjct: 534 SFGQHGPTWWT**GQENKGCEGASC 460
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.141 0.455
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 14,665,021
Number of Sequences: 28460
Number of extensions: 227264
Number of successful extensions: 1589
Number of sequences better than 10.0: 35
Number of HSP's better than 10.0 without gapping: 1575
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1586
length of query: 745
length of database: 4,897,600
effective HSP length: 97
effective length of query: 648
effective length of database: 2,136,980
effective search space: 1384763040
effective search space used: 1384763040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 59 (27.3 bits)
Medicago: description of AC143341.7