BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC143341.1 + phase: 0 /partial
(249 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16999 similar to UP|Q9T024 (Q9T024) DNAJ-like protein (AT4g391... 68 2e-12
AV767621 68 2e-12
TC16983 similar to UP|Q9ZSY2 (Q9ZSY2) ALTERED response to gravit... 66 5e-12
TC12876 64 2e-11
AV411199 62 9e-11
TC8101 similar to UP|O24074 (O24074) DNAJ-like protein, partial ... 62 1e-10
TC8761 homologue to UP|Q9SWB5 (Q9SWB5) Seed maturation protein P... 61 2e-10
TC15224 homologue to UP|Q9SWB5 (Q9SWB5) Seed maturation protein ... 61 2e-10
TC9507 similar to UP|Q8VZB6 (Q8VZB6) DnaJ-like protein, partial ... 60 5e-10
TC13524 similar to UP|O48678 (O48678) F3I6.4 protein, partial (19%) 60 5e-10
AV775648 54 2e-08
CN825120 47 2e-06
TC14831 homologue to UP|P93499 (P93499) DnaJ-like protein (Fragm... 47 4e-06
BP039305 46 5e-06
TC12797 similar to UP|O80799 (O80799) T8F5.5 protein, partial (5%) 38 0.001
BP059356 37 0.002
BP050331 32 0.10
TC19451 similar to GB|AAM65667.1|21593700|AY088122 J8-like prote... 32 0.13
TC19536 similar to UP|BAC84247 (BAC84247) DnaJ protein family-li... 31 0.23
TC8367 similar to GB|AAM65667.1|21593700|AY088122 J8-like protei... 30 0.39
>TC16999 similar to UP|Q9T024 (Q9T024) DNAJ-like protein
(AT4g39150/T22F8_50), partial (35%)
Length = 648
Score = 67.8 bits (164), Expect = 2e-12
Identities = 33/62 (53%), Positives = 40/62 (64%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
VLG+ AS +IKKAYY A +HPDKNPGD +A E FQ L + +L D EKR YD+
Sbjct: 138 VLGVNVDASAADIKKAYYIKARIVHPDKNPGDPKAAENFQMLGEAYQVLSDPEKREAYDK 317
Query: 61 TG 62
G
Sbjct: 318 NG 323
>AV767621
Length = 560
Score = 67.8 bits (164), Expect = 2e-12
Identities = 33/74 (44%), Positives = 52/74 (69%), Gaps = 1/74 (1%)
Frame = +1
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGD-EEAKEKFQQLQKVISILGDEEKRALYD 59
+L +++ AS ++KKAY KLA++ HPDKNP + +EA+ KF+Q+ + +L D +KRA+YD
Sbjct: 73 ILQVDRGASDDDLKKAYRKLAMKWHPDKNPNNKKEAEAKFKQISEAYDVLSDPQKRAVYD 252
Query: 60 QTGCIDDDDLAGDV 73
Q G ++ L G V
Sbjct: 253 QYG---EEGLKGQV 285
>TC16983 similar to UP|Q9ZSY2 (Q9ZSY2) ALTERED response to gravity (ARG1
protein), partial (42%)
Length = 716
Score = 66.2 bits (160), Expect = 5e-12
Identities = 39/94 (41%), Positives = 55/94 (58%), Gaps = 7/94 (7%)
Frame = +2
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
VL + + ++ QEIK AY KLAL+ HPDKN + EA E F+++ SIL D EKR YD
Sbjct: 251 VLSVSRDSTDQEIKTAYRKLALKYHPDKNVNNPEASELFKEVAYSYSILSDPEKRRQYDS 430
Query: 61 TG--CIDDD--DLAGDVQNL---HAFFKTMYKKV 87
G +D D D+ D+ NL + F ++ K+
Sbjct: 431 AGFEALDADGMDMEIDLSNLGTVNTMFAALFSKL 532
>TC12876
Length = 820
Score = 63.9 bits (154), Expect = 2e-11
Identities = 34/74 (45%), Positives = 45/74 (59%), Gaps = 6/74 (8%)
Frame = -1
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGD------EEAKEKFQQLQKVISILGDEEK 54
VLGL K ++ E++ AY KLAL+ HPD+ EEAK+KFQ +Q+ S+L D K
Sbjct: 223 VLGLNKECTESELRNAYKKLALKWHPDRCSASGNLKFVEEAKKKFQSIQEAYSVLSDANK 44
Query: 55 RALYDQTGCIDDDD 68
R +YD G D DD
Sbjct: 43 RLMYD-IGVYDSDD 5
>AV411199
Length = 423
Score = 62.0 bits (149), Expect = 9e-11
Identities = 32/87 (36%), Positives = 58/87 (65%), Gaps = 5/87 (5%)
Frame = +2
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDE-EAKEKFQQLQKVISILGDEEKRALYD 59
+L ++ A+ +E+KKAY +LA++ HPDKNP ++ +A+ KF+ + + +L D +KRA+YD
Sbjct: 80 ILEVDHHATDEELKKAYRRLAMKWHPDKNPDNKNDAETKFKLISESYEVLSDPQKRAIYD 259
Query: 60 QTGCIDDDDLAGDVQN----LHAFFKT 82
+ G +D L G + + + +FF+T
Sbjct: 260 RYG---EDRLKGGMPSPDAGMDSFFRT 331
>TC8101 similar to UP|O24074 (O24074) DNAJ-like protein, partial (90%)
Length = 1737
Score = 61.6 bits (148), Expect = 1e-10
Identities = 33/62 (53%), Positives = 43/62 (69%)
Frame = +1
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
VLG+ K+AS+ EIKKAY K A++ HPDK GD EKF++L + +L D EK+ LYDQ
Sbjct: 196 VLGVPKSASEDEIKKAYRKAAMKNHPDKG-GD---PEKFKELGQAYEVLSDPEKKELYDQ 363
Query: 61 TG 62
G
Sbjct: 364 YG 369
>TC8761 homologue to UP|Q9SWB5 (Q9SWB5) Seed maturation protein PM37,
partial (74%)
Length = 1050
Score = 61.2 bits (147), Expect = 2e-10
Identities = 31/62 (50%), Positives = 42/62 (67%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
+LG+ KTASQ ++KKAY K A++ HPDK GD EKF++L + +L D EKR +YD
Sbjct: 129 ILGVSKTASQDDLKKAYKKAAIKNHPDKG-GD---PEKFKELAQAYEVLNDPEKREIYDN 296
Query: 61 TG 62
G
Sbjct: 297 YG 302
>TC15224 homologue to UP|Q9SWB5 (Q9SWB5) Seed maturation protein PM37,
partial (71%)
Length = 1031
Score = 60.8 bits (146), Expect = 2e-10
Identities = 31/62 (50%), Positives = 42/62 (67%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ 60
+LG+ K ASQ ++KKAY K A++ HPDK GD EKF++L + +L D EKR +YDQ
Sbjct: 186 ILGVPKNASQDDLKKAYKKAAIKNHPDKG-GD---PEKFKELAQAYEVLSDPEKREIYDQ 353
Query: 61 TG 62
G
Sbjct: 354 YG 359
>TC9507 similar to UP|Q8VZB6 (Q8VZB6) DnaJ-like protein, partial (40%)
Length = 905
Score = 59.7 bits (143), Expect = 5e-10
Identities = 29/64 (45%), Positives = 45/64 (70%), Gaps = 2/64 (3%)
Frame = +1
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKN--PGDEEAKEKFQQLQKVISILGDEEKRALY 58
+LGLEK+ + ++++K+Y KL+L++HPDKN PG EEA F+ + K LG+EE + Y
Sbjct: 529 ILGLEKSCTVEDVRKSYRKLSLKVHPDKNKAPGAEEA---FKAVSKAFQCLGNEESKRKY 699
Query: 59 DQTG 62
D +G
Sbjct: 700 DLSG 711
>TC13524 similar to UP|O48678 (O48678) F3I6.4 protein, partial (19%)
Length = 485
Score = 59.7 bits (143), Expect = 5e-10
Identities = 27/59 (45%), Positives = 41/59 (68%)
Frame = +1
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYD 59
VLG+ ++++ QEIK AY K+AL+ HPDKN D +A + F+++ +IL D +KR YD
Sbjct: 304 VLGVSRSSTDQEIKTAYRKMALKYHPDKNANDPKAADMFKEVTFSYNILSDPDKRRQYD 480
>AV775648
Length = 371
Score = 54.3 bits (129), Expect = 2e-08
Identities = 27/59 (45%), Positives = 38/59 (63%)
Frame = +1
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYD 59
++G+ + A+ EIKKAYYKL+L+ HPD NP D E+K+ ++ IL DE R YD
Sbjct: 151 LVGVSQNANSSEIKKAYYKLSLKHHPDTNP-DPESKKLLVKVANAYEILKDEATREQYD 324
>CN825120
Length = 667
Score = 47.4 bits (111), Expect = 2e-06
Identities = 26/63 (41%), Positives = 36/63 (56%), Gaps = 2/63 (3%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGD--EEAKEKFQQLQKVISILGDEEKRALY 58
+LG+ AS QEIK AY +LA HPD D + + +F ++ S L D EKRA Y
Sbjct: 216 ILGIAAAASDQEIKAAYRRLARVRHPDVAAVDRKDSSTNEFMKIHAAYSTLSDPEKRASY 395
Query: 59 DQT 61
D++
Sbjct: 396 DRS 404
>TC14831 homologue to UP|P93499 (P93499) DnaJ-like protein (Fragment),
partial (63%)
Length = 851
Score = 46.6 bits (109), Expect = 4e-06
Identities = 26/63 (41%), Positives = 36/63 (56%), Gaps = 2/63 (3%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEE--AKEKFQQLQKVISILGDEEKRALY 58
+LG+ AS QEIK AY +LA HPD D + + + F ++ S L D EKRA Y
Sbjct: 255 ILGIPAGASSQEIKAAYRRLARVCHPDVAAIDRKNSSADDFMKIHAAYSTLSDPEKRATY 434
Query: 59 DQT 61
D++
Sbjct: 435 DRS 443
>BP039305
Length = 555
Score = 46.2 bits (108), Expect = 5e-06
Identities = 24/56 (42%), Positives = 37/56 (65%), Gaps = 2/56 (3%)
Frame = +3
Query: 2 LGLEKTASQQEIKKAYYKLALRLHPD--KNPGDEEAKEKFQQLQKVISILGDEEKR 55
LG+ K+A+ +EIK AY +LA + HPD K PG A +KF+++ +L D++KR
Sbjct: 396 LGVPKSATVKEIKAAYRRLARQYHPDVYKEPG---ATDKFKEISNAYEVLSDDKKR 554
>TC12797 similar to UP|O80799 (O80799) T8F5.5 protein, partial (5%)
Length = 713
Score = 38.1 bits (87), Expect = 0.001
Identities = 22/54 (40%), Positives = 31/54 (56%)
Frame = +3
Query: 2 LGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKR 55
L L +S E+KK Y K++L +HPDK +AKE F L K +L D+ +R
Sbjct: 372 LNLSFDSSIDEVKKQYRKISLMVHPDKCK-HPQAKEAFGALAKAQQLLLDQNER 530
>BP059356
Length = 436
Score = 37.4 bits (85), Expect = 0.002
Identities = 19/48 (39%), Positives = 31/48 (64%), Gaps = 1/48 (2%)
Frame = +1
Query: 27 DKNPGDE-EAKEKFQQLQKVISILGDEEKRALYDQTGCIDDDDLAGDV 73
DKNP ++ EA+ F+++ + +L D +KRA+YDQ G ++ L G V
Sbjct: 184 DKNPNNKKEAEANFKKISEAYDVLSDPQKRAVYDQFG---EEGLNGQV 318
Score = 33.5 bits (75), Expect = 0.035
Identities = 16/42 (38%), Positives = 27/42 (64%), Gaps = 1/42 (2%)
Frame = +3
Query: 1 VLGLEKTASQQEIKKAYYKLALRLHPDKNPGDEEAK-EKFQQ 41
VL ++++A ++KKAY KLA++ HP P +E +FQ+
Sbjct: 105 VLQVDRSAKDDDLKKAYRKLAMKWHPG*EP*QQERS*SQFQE 230
>BP050331
Length = 519
Score = 32.0 bits (71), Expect = 0.10
Identities = 19/52 (36%), Positives = 28/52 (53%), Gaps = 1/52 (1%)
Frame = +3
Query: 13 IKKAYYKLALRLHPDKNPGDEEAKEKFQQLQKVISILGDEEKRALYDQ-TGC 63
IK+ Y +LAL LHPDKN + F + ++L D ++ +YD GC
Sbjct: 321 IKRQYRRLALLLHPDKNL-FSFSHHTFDLVSHAWALLSDPAQKEIYDAGLGC 473
>TC19451 similar to GB|AAM65667.1|21593700|AY088122 J8-like protein
{Arabidopsis thaliana;}, partial (35%)
Length = 480
Score = 31.6 bits (70), Expect = 0.13
Identities = 15/32 (46%), Positives = 21/32 (64%)
Frame = +2
Query: 2 LGLEKTASQQEIKKAYYKLALRLHPDKNPGDE 33
L ++ ASQ E+KKA+ LAL+ HPD G +
Sbjct: 242 LRVQPGASQTEVKKAFRHLALQYHPDVCQGSK 337
>TC19536 similar to UP|BAC84247 (BAC84247) DnaJ protein family-like
protein, partial (27%)
Length = 597
Score = 30.8 bits (68), Expect = 0.23
Identities = 20/59 (33%), Positives = 30/59 (49%), Gaps = 5/59 (8%)
Frame = +3
Query: 1 VLGLEKTA----SQQEIKKAYYKLALRLHPDKNPGDEE-AKEKFQQLQKVISILGDEEK 54
VLGL++ S EIK A+ A HPD+N + E A+ KF+++ + E K
Sbjct: 24 VLGLDRFRQAPYSDAEIKSAFRTKAKEHHPDQNQNNREVAEAKFKEVMSSYEAIKQERK 200
>TC8367 similar to GB|AAM65667.1|21593700|AY088122 J8-like protein
{Arabidopsis thaliana;}, partial (64%)
Length = 962
Score = 30.0 bits (66), Expect = 0.39
Identities = 12/26 (46%), Positives = 20/26 (76%)
Frame = +2
Query: 2 LGLEKTASQQEIKKAYYKLALRLHPD 27
L ++ AS+ E++KA+ +LAL+ HPD
Sbjct: 440 LKIQPGASETEVRKAFRQLALQYHPD 517
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.310 0.128 0.349
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,334,351
Number of Sequences: 28460
Number of extensions: 35266
Number of successful extensions: 222
Number of sequences better than 10.0: 56
Number of HSP's better than 10.0 without gapping: 213
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 214
length of query: 249
length of database: 4,897,600
effective HSP length: 88
effective length of query: 161
effective length of database: 2,393,120
effective search space: 385292320
effective search space used: 385292320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC143341.1