BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC143339.8 + phase: 0 /pseudo
(518 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV412895 63 1e-10
BP041113 55 2e-08
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 52 2e-07
AV428268 50 7e-07
BP056597 49 3e-06
AV414139 47 8e-06
AV411435 47 1e-05
AV767121 46 2e-05
BP028574 45 4e-05
AV780705 44 8e-05
BE122611 37 2e-04
AU089037 40 7e-04
TC18710 39 0.002
AV777945 37 0.006
TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane pro... 35 0.030
AV418280 30 0.73
TC11455 similar to UP|Q9ZUS8 (Q9ZUS8) At2g37380 protein, partial... 30 1.2
TC13572 29 1.6
TC7938 homologue to UP|Q8LJW0 (Q8LJW0) 40S ribosomal S4 protein,... 29 1.6
AV414649 28 3.6
>AV412895
Length = 405
Score = 62.8 bits (151), Expect = 1e-10
Identities = 33/103 (32%), Positives = 55/103 (53%)
Frame = +1
Query: 416 GYSVNGVYQMLTSQPVQTSEVLLDLIWHKQVPLKVSIFAWRLLQNRLPTKDNLCVHGVIP 475
G+SVN + L Q ++ + + + IW PL + F WR+L R+ T+DNL VI
Sbjct: 7 GFSVNSSFVFL*DQYLEEPDPVFN*IWMVPAPLNIKAFVWRVLLGRIQTRDNLLKRQVIH 186
Query: 476 HSHDNQHCVIGCGDFETAQHLFRSCPFYVALWGQIRSWLGIAT 518
++ D + G + E+ HL SC + +W + +WLG++T
Sbjct: 187 NALDAICPLCGLAE-ESGSHLLFSCAESMLIWYECFAWLGVST 312
>BP041113
Length = 503
Score = 55.5 bits (132), Expect = 2e-08
Identities = 43/138 (31%), Positives = 59/138 (42%), Gaps = 14/138 (10%)
Frame = +1
Query: 373 GGWQ---------WRRRLWAWEEELLGECRILLSDIT---LQH--TDRWVWRLDPSNGYS 418
GGW WRR L E LG +++ D++ L D+W+W P Y+
Sbjct: 100 GGWNHGVWTWKLSWRRALVGRE---LGWLELMMKDLSGVCLSEGVEDKWLWL--PGGTYT 264
Query: 419 VNGVYQMLTSQPVQTSEVLLDLIWHKQVPLKVSIFAWRLLQNRLPTKDNLCVHGVIPHSH 478
VN Y L + + ++ + IW P V FAWR L RLPT DNL V+
Sbjct: 265 VNSAYSFLQAPTLADTDPIFATIWRTVAP-SVKAFAWRCLLGRLPTYDNLIKRQVVVDPA 441
Query: 479 DNQHCVIGCGDFETAQHL 496
C G+ E+ HL
Sbjct: 442 MTV-CKFCQGEVESVTHL 492
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 52.0 bits (123), Expect = 2e-07
Identities = 42/125 (33%), Positives = 63/125 (49%)
Frame = -3
Query: 68 ISHLQFADDTLLVGVKSWANVRALRAVLLLLR*CRSGLKMNFHKSLLVGVNVSESWLNEA 127
ISHL FAD+ ++ ++ + VL + + SGL N KS V + V E+ N+
Sbjct: 373 ISHLCFADNLMVFSNGYLESIAIINNVLHIFQHL-SGLTPNPAKSE-VFILVKETTKNQI 200
Query: 128 TTMLGCKVGKVPFLYLGLSIGGDPRRLLFWESVVDRVKKRLSGWRSKFLSFGGRLVLLKV 187
T MLG K GK+P LG+ + + +VDR +L W + LS+ GRL L+
Sbjct: 199 TNMLGYKEGKLPVR*LGVPQLTTKLKASDCKVLVDRKPSKLQHWTGRSLSYAGRLQLINS 20
Query: 188 FLSSL 192
L S+
Sbjct: 19 ILFSM 5
>AV428268
Length = 429
Score = 50.4 bits (119), Expect = 7e-07
Identities = 30/100 (30%), Positives = 45/100 (45%)
Frame = +3
Query: 417 YSVNGVYQMLTSQPVQTSEVLLDLIWHKQVPLKVSIFAWRLLQNRLPTKDNLCVHGVIPH 476
YSV Y L + + L+W +VP +W++L NR+ TK NL GV+
Sbjct: 6 YSVKSAYDFLHGCANAQWDNIFKLLWSVKVPSNAIALSWKVLINRVQTKVNLNRRGVV-- 179
Query: 477 SHDNQHCVIGCGDFETAQHLFRSCPFYVALWGQIRSWLGI 516
+ C + D E+ HL SCP +W +I G+
Sbjct: 180 --ISNVCPLCSLDEESTDHLLFSCPIV*RIWSKISE*FGV 293
>BP056597
Length = 414
Score = 48.5 bits (114), Expect = 3e-06
Identities = 28/105 (26%), Positives = 44/105 (41%), Gaps = 13/105 (12%)
Frame = +3
Query: 377 WRRRLWAWE---------EELL--GECRILLSDITLQH--TDRWVWRLDPSNGYSVNGVY 423
W W W+ E++L G + ++S +L + D WVW L+ YSV Y
Sbjct: 69 WLENKWVWDFSWKNPISGEDVLKLGVMKQIVSSFSLINGKKDTWVWILEGDGQYSVKSAY 248
Query: 424 QMLTSQPVQTSEVLLDLIWHKQVPLKVSIFAWRLLQNRLPTKDNL 468
+L+ + +W P WR+ +R+ TKDNL
Sbjct: 249 DLLSGLDTTIGISVFSKLWKACAPSNAVALGWRVFLDRIQTKDNL 383
>AV414139
Length = 309
Score = 47.0 bits (110), Expect = 8e-06
Identities = 25/86 (29%), Positives = 39/86 (45%), Gaps = 2/86 (2%)
Frame = -2
Query: 375 WQWRRRLWAWEEELLGECRILLSDITLQH--TDRWVWRLDPSNGYSVNGVYQMLTSQPVQ 432
++WRR L E+ + L+ + LQ DRW W LD + Y+V Y +L
Sbjct: 263 FRWRRELSVREQRQEADLIQALAGVCLQQGTEDRWKWTLDTEDIYTVRSAY*LLLDPIAD 84
Query: 433 TSEVLLDLIWHKQVPLKVSIFAWRLL 458
+ + + +W P + F WRLL
Sbjct: 83 SDSEVYEKVWVGIAPSNANAFVWRLL 6
>AV411435
Length = 426
Score = 46.6 bits (109), Expect = 1e-05
Identities = 35/113 (30%), Positives = 42/113 (36%), Gaps = 10/113 (8%)
Frame = +3
Query: 405 DRWVWRLDPSNGYSVNGVYQM----------LTSQPVQTSEVLLDLIWHKQVPLKVSIFA 454
DR W PS YSV Y+ L S S L IW QVP KV F
Sbjct: 39 DRLFWPFIPSGEYSVKSGYRAVKATQFNLSNLASSSSHPSSFLWHTIWGAQVPKKVRSFL 218
Query: 455 WRLLQNRLPTKDNLCVHGVIPHSHDNQHCVIGCGDFETAQHLFRSCPFYVALW 507
WR N +P K NL + + C I E H +C + A+W
Sbjct: 219 WRAANNAIPVKRNLKRRNM----GRDDFCPICNKGPEDINHALLACEWTRAVW 365
>AV767121
Length = 525
Score = 45.8 bits (107), Expect = 2e-05
Identities = 22/54 (40%), Positives = 34/54 (62%)
Frame = +2
Query: 168 LSGWRSKFLSFGGRLVLLKVFLSSLHVYALSFFRAPSGIISTLESLFINFFLVG 221
LS W+ K +SFGGR+ L++ L++L ++ LSFF+ P G+ + L NF G
Sbjct: 2 LSKWKQKTVSFGGRIFLIQSVLTALPLFFLSFFKLPIGVGKSCVRLMRNFLWGG 163
>BP028574
Length = 454
Score = 44.7 bits (104), Expect = 4e-05
Identities = 18/65 (27%), Positives = 31/65 (47%)
Frame = +3
Query: 399 ITLQHTDRWVWRLDPSNGYSVNGVYQMLTSQPVQTSEVLLDLIWHKQVPLKVSIFAWRLL 458
+++ H D WVW D S ++V Y+ L + + + +W + P AWR+
Sbjct: 243 LSINHRDSWVWIKDGSGSFTVKSAYEELHGEIQEIEDNFFRKLWQSKAPSNSIALAWRVG 422
Query: 459 QNRLP 463
NR+P
Sbjct: 423 LNRVP 437
>AV780705
Length = 524
Score = 43.5 bits (101), Expect = 8e-05
Identities = 24/82 (29%), Positives = 44/82 (53%), Gaps = 2/82 (2%)
Frame = -2
Query: 139 PFLYLGLSI--GGDPRRLLFWESVVDRVKKRLSGWRSKFLSFGGRLVLLKVFLSSLHVYA 196
P YLGL G + L W + ++VK++L GW+ L+ G+ VL+K + ++ YA
Sbjct: 493 PGHYLGLPAIWGRNKSHSLVW--IEEKVKEKLEGWKETLLNQAGKEVLIKAIIQAIPSYA 320
Query: 197 LSFFRAPSGIISTLESLFINFF 218
++ P ++L +L +F+
Sbjct: 319 MTIVHFPKTFCNSLYALVADFW 254
>BE122611
Length = 357
Score = 37.0 bits (84), Expect(2) = 2e-04
Identities = 19/61 (31%), Positives = 27/61 (44%)
Frame = -3
Query: 404 TDRWVWRLDPSNGYSVNGVYQMLTSQPVQTSEVLLDLIWHKQVPLKVSIFAWRLLQNRLP 463
TDR +W + + YS Y +L S L+W P KV F W + +N LP
Sbjct: 334 TDRILWTEESNGCYSAKSTYNLLIEDGGNESR-FWSLVWKANAPEKVRFFLWLVGRNALP 158
Query: 464 T 464
+
Sbjct: 157 S 155
Score = 24.6 bits (52), Expect(2) = 2e-04
Identities = 13/36 (36%), Positives = 17/36 (47%), Gaps = 1/36 (2%)
Frame = -2
Query: 475 PHSHDNQHCVIGCG-DFETAQHLFRSCPFYVALWGQ 509
P+ + I CG E A H+FR CP +W Q
Sbjct: 140 PYHLASSAACIRCGAPSEDANHVFRWCPGSAWMWTQ 33
>AU089037
Length = 245
Score = 40.4 bits (93), Expect = 7e-04
Identities = 27/77 (35%), Positives = 39/77 (50%)
Frame = -1
Query: 425 MLTSQPVQTSEVLLDLIWHKQVPLKVSIFAWRLLQNRLPTKDNLCVHGVIPHSHDNQHCV 484
+L S V +S++ L W P FAWRL+ +R+ T+ NL VI S + C
Sbjct: 236 LLGSIQVPSSDIFQKL-WKVPAPSNAVSFAWRLILDRVQTRGNLRRRQVIQQSEEAL-CP 63
Query: 485 IGCGDFETAQHLFRSCP 501
+ E++ HLF SCP
Sbjct: 62 MCSQCEESSSHLFFSCP 12
>TC18710
Length = 843
Score = 38.9 bits (89), Expect = 0.002
Identities = 17/39 (43%), Positives = 27/39 (68%)
Frame = +2
Query: 10 VNGSPTPEFPLERGLRKGDPLSQFLFLLAAEGLNVMMQS 48
+NG P+ +RGLR+GDP S +LFL E L++++Q+
Sbjct: 704 INGVVGPKLLPQRGLRQGDPFSPYLFLFTMEVLSLLIQN 820
>AV777945
Length = 279
Score = 37.4 bits (85), Expect = 0.006
Identities = 21/69 (30%), Positives = 33/69 (47%)
Frame = +1
Query: 411 LDPSNGYSVNGVYQMLTSQPVQTSEVLLDLIWHKQVPLKVSIFAWRLLQNRLPTKDNLCV 470
LDP + + + + L + +++ E L D +W + P AW +L +L TKD L
Sbjct: 76 LDPKSNF*AS*LLTQL--KQLRSHEGLFDFLWASKAPSNSLALAWNVLIKKLQTKDELRK 249
Query: 471 HGVIPHSHD 479
VIP D
Sbjct: 250 RNVIPSGTD 276
>TC15408 similar to UP|Q9LIC2 (Q9LIC2) Multispanning membrane protein-like,
partial (10%)
Length = 879
Score = 35.0 bits (79), Expect = 0.030
Identities = 22/78 (28%), Positives = 36/78 (45%), Gaps = 6/78 (7%)
Frame = -1
Query: 371 MKGGWQW---RRRLWAWEEELLGE--CRILLSDITLQHT-DRWVWRLDPSNGYSVNGVYQ 424
++G W W RRR + E LL C +L S + T D W W +P Y+V Y+
Sbjct: 780 VEGVWTWSFIRRRNPSTRENLLVADLCNLLESVPLSEDTNDSWSWTANPEGQYTVQTAYK 601
Query: 425 MLTSQPVQTSEVLLDLIW 442
L + + ++ + +W
Sbjct: 600 FLRASSNEFTDPIFHFVW 547
>AV418280
Length = 398
Score = 30.4 bits (67), Expect = 0.73
Identities = 11/27 (40%), Positives = 17/27 (62%)
Frame = -2
Query: 491 ETAQHLFRSCPFYVALWGQIRSWLGIA 517
E + HLF +C F + +W + WLGI+
Sbjct: 364 ECSGHLFFTCVFSMGVWQALHRWLGIS 284
>TC11455 similar to UP|Q9ZUS8 (Q9ZUS8) At2g37380 protein, partial (10%)
Length = 575
Score = 29.6 bits (65), Expect = 1.2
Identities = 26/80 (32%), Positives = 34/80 (42%), Gaps = 12/80 (15%)
Frame = -2
Query: 328 RLTRRFGWIR----GWAVRHCA-----FGLGVCLT*LSINLFRWQICVS*DEMKGGWQW- 377
R+ RR W R G C G G+C RW+IC E++ W+W
Sbjct: 325 RMRRRMRWRRRRGGGCGPWRCGEKGGEVGAGLCRRVHRQGRVRWKIC----EVR--WRW* 164
Query: 378 --RRRLWAWEEELLGECRIL 395
R++ E L GECRIL
Sbjct: 163 IRSRKMKKSERVLKGECRIL 104
>TC13572
Length = 534
Score = 29.3 bits (64), Expect = 1.6
Identities = 21/72 (29%), Positives = 31/72 (42%), Gaps = 14/72 (19%)
Frame = -3
Query: 373 GGW---------QWRRRLWAWEEELLGECRILLSDITLQH--TDRWVWRLDPSNGYSVNG 421
GGW +WRR L E + L + + + L D+WVW+ YSVN
Sbjct: 229 GGWSQGVWNWDFRWRRPLAGRELDWLQALSLDIVRVPLLAGVPDKWVWKPSEDGSYSVNS 50
Query: 422 VY---QMLTSQP 430
+ Q+ + QP
Sbjct: 49 AFIFLQVPSEQP 14
>TC7938 homologue to UP|Q8LJW0 (Q8LJW0) 40S ribosomal S4 protein, complete
Length = 1142
Score = 29.3 bits (64), Expect = 1.6
Identities = 14/31 (45%), Positives = 20/31 (64%)
Frame = -3
Query: 455 WRLLQNRLPTKDNLCVHGVIPHSHDNQHCVI 485
W LL +RL TK L +G IP H+N+ C++
Sbjct: 1044 WNLLPSRLETKYKLD-NGKIPSWHENKSCMV 955
>AV414649
Length = 297
Score = 28.1 bits (61), Expect = 3.6
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -3
Query: 377 WRRRLWAWEEELLGE 391
WRR W W+EE +GE
Sbjct: 55 WRRSGWLWQEEAMGE 11
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.338 0.150 0.517
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,311,382
Number of Sequences: 28460
Number of extensions: 195081
Number of successful extensions: 1674
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 1630
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1670
length of query: 518
length of database: 4,897,600
effective HSP length: 94
effective length of query: 424
effective length of database: 2,222,360
effective search space: 942280640
effective search space used: 942280640
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC143339.8