BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC143337.6 + phase: 0
(276 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8302 48 2e-06
TC9039 43 5e-05
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 38 0.002
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 37 0.003
BI418821 37 0.004
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 37 0.004
AV420911 37 0.004
AV773507 35 0.014
AV421607 33 0.053
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 33 0.069
TC17929 31 0.20
BP065763 31 0.20
TC12773 30 0.58
BP039068 29 0.76
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 29 1.00
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 28 1.3
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 28 1.3
AI967518 28 1.7
TC11026 similar to UP|AAO25536 (AAO25536) Cellulose synthase, pa... 28 2.2
TC17953 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17... 27 2.9
>TC8302
Length = 494
Score = 47.8 bits (112), Expect = 2e-06
Identities = 36/137 (26%), Positives = 66/137 (47%), Gaps = 5/137 (3%)
Frame = +3
Query: 104 QQLYSFRMVESKAIMEQLTEFNKILDDLENIEVQLEDEDKAILLLCALPKSFE----SFK 159
++LY+ RM ES ++ + + N + L + + ++A LLL +LP S++ + K
Sbjct: 3 KRLYTLRMSESTSMSDHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSYDQLVINIK 182
Query: 160 DTMLYGKEGTVTLEEVQAALRTKDLTKS-KDLTHEHGEGLSVTRGNGGGRGNRRKSGNKS 218
+ + + L EV ++ K + + + E L++TRG R KS K
Sbjct: 183 NNNIVNHLPLMMLSEVLLEEDSQR*NKEDR*DSSKPMEALTMTRG-------RSKSRKKI 341
Query: 219 RFECFNCHKMGHFKKDC 235
+C++C + H KKDC
Sbjct: 342 NLKCYHCGQR*HLKKDC 392
>TC9039
Length = 1218
Score = 43.1 bits (100), Expect = 5e-05
Identities = 37/166 (22%), Positives = 62/166 (37%), Gaps = 8/166 (4%)
Frame = +2
Query: 80 AASMWAKLESLYMTKSLAHRQFLKQQLYSFRMVESKAIMEQLTEFNKILDDLENIEVQLE 139
A A++E + A L Q L S + I E + + I L+ ++++L
Sbjct: 395 AKDFLAEIEKRFAKSDKAETSTLLQNLISMKYQGKGNIREYIMGMSNIASKLKALKLELS 574
Query: 140 DEDKAILLLCALPKSFESFKDTMLYGKEGTVTLEEVQAALRTKDLTKSKDLTHEHGEGLS 199
D+ L+L +LP F FK + KE E + ++ ++ K + H S
Sbjct: 575 DDLLIHLVLLSLPAQFSQFKISYNCPKEKWSLNELISFCVQEEERLKQERKESAHFVSTS 754
Query: 200 VTRGNGGGRGNRRKSG--------NKSRFECFNCHKMGHFKKDCPK 237
+G + K C+ C+ GH KK C K
Sbjct: 755 KDKGKRKKTVEPKNEAADAPAPKKQKEDDTCYFCNVSGHMKKKCTK 892
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 37.7 bits (86), Expect = 0.002
Identities = 15/41 (36%), Positives = 24/41 (57%)
Frame = +1
Query: 201 TRGNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCPKINGN 241
++G GGGRG R G + +C+ C + GHF ++C G+
Sbjct: 106 SKGGGGGRGGGRGRGGED-LKCYECGEPGHFARECRSRGGS 225
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 37.4 bits (85), Expect = 0.003
Identities = 27/93 (29%), Positives = 40/93 (42%), Gaps = 6/93 (6%)
Frame = +2
Query: 150 ALPKSFESFKDTMLYGKEGTVTLEEVQAALRTKDLTKSKDLTHEHGEGLSV------TRG 203
A+ ++ ++ L G+ TV + Q + R +D D E G +RG
Sbjct: 215 AMDEAIDAMNGMDLDGRTITVDKAQPQGSSRDRD---DGDRYRERGRDRDDRGDRDRSRG 385
Query: 204 NGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCP 236
GG RG+ ECF C K GHF ++CP
Sbjct: 386 YGGSRGSNGG-------ECFKCGKPGHFARECP 463
>BI418821
Length = 614
Score = 37.0 bits (84), Expect = 0.004
Identities = 29/108 (26%), Positives = 37/108 (33%), Gaps = 37/108 (34%)
Frame = +2
Query: 172 LEEVQAALRTKDLTKSKDLTHEHGEGLSVTR------GNGGGRGNRRKSGNKSR------ 219
L E A D TK+ D+T +G L TR G GG RG R++G
Sbjct: 215 LVEFSIATGDNDKTKAVDVTGPNGAALQPTRKDSAPRGFGGWRGGERRNGGGGGGGGGGC 394
Query: 220 -------------------------FECFNCHKMGHFKKDCPKINGNS 242
C+NC GH +DC + N NS
Sbjct: 395 YNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNS 538
Score = 26.9 bits (58), Expect = 3.8
Identities = 11/32 (34%), Positives = 15/32 (46%)
Frame = +2
Query: 208 RGNRRKSGNKSRFECFNCHKMGHFKKDCPKIN 239
R N G + C+NC GH +DC + N
Sbjct: 521 RSNNNSGGGGAG--CYNCGDTGHLARDCNRSN 610
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 37.0 bits (84), Expect = 0.004
Identities = 17/44 (38%), Positives = 25/44 (56%), Gaps = 2/44 (4%)
Frame = +1
Query: 203 GNGGGRGNRRKSGNK--SRFECFNCHKMGHFKKDCPKINGNSAQ 244
G GGGRG R G S +C+ C + GHF ++C ++ G S +
Sbjct: 316 GGGGGRGGGRSGGGGGGSDMKCYECGEPGHFAREC-RMRGGSGR 444
>AV420911
Length = 418
Score = 37.0 bits (84), Expect = 0.004
Identities = 14/35 (40%), Positives = 22/35 (62%)
Frame = +2
Query: 201 TRGNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDC 235
++G GGGRG R G + +C+ C + GHF ++C
Sbjct: 314 SKGGGGGRGGGRGRGGED-LKCYECGEPGHFAREC 415
>AV773507
Length = 496
Score = 35.0 bits (79), Expect = 0.014
Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 3/46 (6%)
Frame = +2
Query: 202 RGNG---GGRGNRRKSGNKSRFECFNCHKMGHFKKDCPKINGNSAQ 244
RG G GGRG+ + +CF C + GH +DCP G +
Sbjct: 281 RGGGYSSGGRGSYGAGDRVGQDDCFECGRPGHRARDCPLAGGGGGR 418
>AV421607
Length = 245
Score = 33.1 bits (74), Expect = 0.053
Identities = 15/40 (37%), Positives = 22/40 (54%)
Frame = +3
Query: 203 GNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCPKINGNS 242
G+G G G SG+ + C+ C + GH+ +DCP NS
Sbjct: 9 GSGPGSG----SGSGTATGCYKCGRPGHWSRDCPSSAPNS 116
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 32.7 bits (73), Expect = 0.069
Identities = 17/44 (38%), Positives = 20/44 (44%), Gaps = 3/44 (6%)
Frame = +2
Query: 195 GEGLSVTRGNGGGRGNRRKSGN---KSRFECFNCHKMGHFKKDC 235
G + V GG RGNR G CFNC GH+ +DC
Sbjct: 281 GSRIVVEFAKGGPRGNREYLGRGPPPGSGRCFNCGLDGHWARDC 412
>TC17929
Length = 791
Score = 31.2 bits (69), Expect = 0.20
Identities = 13/44 (29%), Positives = 23/44 (51%)
Frame = +2
Query: 202 RGNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCPKINGNSAQI 245
R G + R + +K R C+ C + GH ++CPK + + +I
Sbjct: 2 RAQIGEETSNRPNDSKFRQTCYRCGESGHKMRNCPKEHSSGEKI 133
>BP065763
Length = 537
Score = 31.2 bits (69), Expect = 0.20
Identities = 11/16 (68%), Positives = 12/16 (74%)
Frame = -1
Query: 222 CFNCHKMGHFKKDCPK 237
C+ C K GHFKK CPK
Sbjct: 381 CYFCKKAGHFKKYCPK 334
>TC12773
Length = 420
Score = 29.6 bits (65), Expect = 0.58
Identities = 12/30 (40%), Positives = 17/30 (56%), Gaps = 4/30 (13%)
Frame = +2
Query: 210 NRRKSGNK----SRFECFNCHKMGHFKKDC 235
NR + NK S +EC+ C + GH +DC
Sbjct: 299 NRHRWSNKRSSLSTYECWKCQRPGHMAEDC 388
>BP039068
Length = 467
Score = 29.3 bits (64), Expect = 0.76
Identities = 11/37 (29%), Positives = 15/37 (39%)
Frame = +3
Query: 202 RGNGGGRGNRRKSGNKSRFECFNCHKMGHFKKDCPKI 238
RG + N + C NC + GH DCP +
Sbjct: 252 RGPTASTSSNVNHNNPRQTVCMNCQETGHASNDCPSL 362
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 28.9 bits (63), Expect = 1.00
Identities = 23/91 (25%), Positives = 33/91 (35%), Gaps = 19/91 (20%)
Frame = +1
Query: 164 YGKEGTVTLEEVQAALRTKDLTKSKDLTHE------HGEGLSVTRGNGGGRGNRRKSGNK 217
YG+ V ++ A + D + D + G L V G RG+R G +
Sbjct: 160 YGRVRDVDMKRDYAFVEFSDPRDADDARYNLDGRDVDGSRLIVEFAKGVPRGSREGGGGR 339
Query: 218 SRFE-------------CFNCHKMGHFKKDC 235
R CFNC GH+ +DC
Sbjct: 340 DRDREYMGRGPPPGSGRCFNCGIDGHWARDC 432
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 28.5 bits (62), Expect = 1.3
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +2
Query: 222 CFNCHKMGHFKKDCPKI 238
C NC + GHF ++CP +
Sbjct: 344 CKNCKRPGHFARECPNV 394
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 28.5 bits (62), Expect = 1.3
Identities = 12/28 (42%), Positives = 16/28 (56%), Gaps = 2/28 (7%)
Frame = -2
Query: 222 CFNCHKMGHFKKDC--PKINGNSAQIVS 247
C+NC K GH KDC PK+ + I +
Sbjct: 275 CWNCQKTGHSGKDCTNPKVEAATNAIAA 192
Score = 26.9 bits (58), Expect = 3.8
Identities = 10/28 (35%), Positives = 12/28 (42%)
Frame = -2
Query: 211 RRKSGNKSRFECFNCHKMGHFKKDCPKI 238
R + S C C K GHF CP +
Sbjct: 362 RPTQSDTSEIVCHRCSKKGHFANRCPDL 279
>AI967518
Length = 451
Score = 28.1 bits (61), Expect = 1.7
Identities = 13/42 (30%), Positives = 24/42 (56%)
Frame = +2
Query: 206 GGRGNRRKSGNKSRFECFNCHKMGHFKKDCPKINGNSAQIVS 247
GGR ++++ +++R NC K GH + CPK G + ++
Sbjct: 14 GGRPSKKRY-SRTRRRRTNCRKTGHTLRTCPKRIGTKDEHIA 136
>TC11026 similar to UP|AAO25536 (AAO25536) Cellulose synthase, partial (25%)
Length = 946
Score = 27.7 bits (60), Expect = 2.2
Identities = 18/49 (36%), Positives = 25/49 (50%), Gaps = 1/49 (2%)
Frame = +1
Query: 19 WKVKMEAVLIQQKCEKALKGEGSLPVTMSRAEKTKMVDKARSA-VVLCL 66
WK+K E ++Q + G + T S E+ +MVD AR VV CL
Sbjct: 739 WKLKQEKNMVQMTGKYPEGKGGDIEGTGSNGEELQMVDDARHL*VV*CL 885
>TC17953 similar to GB|AAM26637.1|20855902|AY101516 AT3g06940/F17A9_9
{Arabidopsis thaliana;}, partial (6%)
Length = 527
Score = 27.3 bits (59), Expect = 2.9
Identities = 16/66 (24%), Positives = 28/66 (42%), Gaps = 10/66 (15%)
Frame = +3
Query: 217 KSRFECFNCHKMGHFKKDCPK----------INGNSAQIVSEGYEDAGALMVWCCLEEEK 266
K + +C C ++GH KK C I+ +S +V++ L W C +
Sbjct: 108 KRQLQCSKCKELGHNKKTCKNSLPLEQDT*AISNHSRDLVNK------TLRGWSCFQSTH 269
Query: 267 GDVSHL 272
D +H+
Sbjct: 270 LD*NHV 287
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.132 0.382
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,195,643
Number of Sequences: 28460
Number of extensions: 46601
Number of successful extensions: 331
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 321
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 329
length of query: 276
length of database: 4,897,600
effective HSP length: 89
effective length of query: 187
effective length of database: 2,364,660
effective search space: 442191420
effective search space used: 442191420
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC143337.6