BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC142526.4 + phase: 0
(187 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI418527 55 1e-08
TC14982 similar to UP|Q9FPI2 (Q9FPI2) At1g17620, partial (32%) 47 2e-06
AV427017 41 1e-04
TC9679 weakly similar to UP|Q8H1B5 (Q8H1B5) Hin1-like protein, p... 40 2e-04
TC8092 similar to UP|Q8MLI6 (Q8MLI6) CG30127-PA, partial (3%) 33 0.023
BP028899 33 0.030
AV409132 28 0.97
TC8982 similar to UP|Q84QW4 (Q84QW4) OJ1191_A10.2 protein, parti... 27 2.2
TC19441 27 2.2
CN825801 27 2.8
BP078290 26 3.7
TC14635 similar to GB|AAO44074.1|28466931|BT004808 At5g11090 {Ar... 26 4.8
BP043338 25 6.3
BP061066 25 6.3
TC19681 similar to AAQ56809 (AAQ56809) At4g12700, partial (18%) 25 6.3
BI417934 25 6.3
NP840539 hypothetical protein [Lotus corniculatus var. japonicus] 25 6.3
NP566647 unnamed protein product [Lotus japonicus] 25 6.3
BP043776 25 6.3
TC14760 weakly similar to UP|Q8GUU3 (Q8GUU3) FEG protein, partia... 25 8.2
>BI418527
Length = 482
Score = 54.7 bits (130), Expect = 1e-08
Identities = 31/105 (29%), Positives = 56/105 (52%), Gaps = 3/105 (2%)
Frame = +3
Query: 1 MTKHEHETPPSNRTNLASCLVATVFLIFILIIIFTLYFTLFKPQDPKISVTAVQLPSFNL 60
+++H H+ S R + VFL+ + ++I L FT+F+ ++P I + ++Q+ + NL
Sbjct: 162 LSQHHHQKL-SRRHFIKRFACPLVFLLLLAVVIIVLIFTVFRIKNPVIKMNSIQITNLNL 338
Query: 61 TN---NSTTVNFTFSQYTSVKNPNRGTFSHYDSSFQLLSYGKQIG 102
TN VN + SVKNPN +F + +++ L G +G
Sbjct: 339 TNIVAAKPGVNLSVIADVSVKNPNAASFRYSNTTTSLYYRGVMVG 473
>TC14982 similar to UP|Q9FPI2 (Q9FPI2) At1g17620, partial (32%)
Length = 1036
Score = 47.0 bits (110), Expect = 2e-06
Identities = 31/125 (24%), Positives = 59/125 (46%), Gaps = 10/125 (8%)
Frame = +2
Query: 10 PSNRTNLASCLVATVFLIFILIIIFTL--------YFTLFKPQDPKISVTAVQLPSFNLT 61
P +R C F + ++I+I L ++ L++P P SVT+++L NLT
Sbjct: 158 PLHRRTRRRCFCTFCFWLILIILILLLIIGGAGVAFYLLYRPHHPTFSVTSLKLSYLNLT 337
Query: 62 NNSTTVNFTFSQYTSVKNPNRG--TFSHYDSSFQLLSYGKQIGFMFVPAGKINARRTQFM 119
+S+T++ F + NPN+ FS+ +S +LS +G +P + T +
Sbjct: 338 -SSSTLSSKFDLTVAATNPNKKNIAFSYLPTSITILSGDVDVGDGTIPTFHHGKKNTTLL 514
Query: 120 AATFT 124
++ +
Sbjct: 515 KSSIS 529
>AV427017
Length = 429
Score = 40.8 bits (94), Expect = 1e-04
Identities = 24/80 (30%), Positives = 40/80 (50%), Gaps = 5/80 (6%)
Frame = +2
Query: 16 LASCLVATVFLIFILIIIFT-----LYFTLFKPQDPKISVTAVQLPSFNLTNNSTTVNFT 70
+A+C++ + I III L++ + +P K VT L F TNN T+++
Sbjct: 140 IANCILGLICKILTTIIIIAAVAGFLFWLIVRPNVVKFRVTEASLTEFTYTNN--TLHYN 313
Query: 71 FSQYTSVKNPNRGTFSHYDS 90
+ +V+NPN +YDS
Sbjct: 314 LALNITVRNPNSRVGLYYDS 373
>TC9679 weakly similar to UP|Q8H1B5 (Q8H1B5) Hin1-like protein, partial
(54%)
Length = 710
Score = 40.4 bits (93), Expect = 2e-04
Identities = 24/76 (31%), Positives = 41/76 (53%), Gaps = 5/76 (6%)
Frame = +2
Query: 19 CLVATVF-LIFILIIIF----TLYFTLFKPQDPKISVTAVQLPSFNLTNNSTTVNFTFSQ 73
C+ + +F LIF +II +++ + +P K VT L FN T N+T +N+ +
Sbjct: 170 CIFSLIFKLIFTALIIIGIAVLIFWLIVRPNVVKFHVTDATLTQFNFTGNNT-LNYDLTL 346
Query: 74 YTSVKNPNRGTFSHYD 89
+V+NPN+ +YD
Sbjct: 347 NITVRNPNKRLGIYYD 394
>TC8092 similar to UP|Q8MLI6 (Q8MLI6) CG30127-PA, partial (3%)
Length = 1073
Score = 33.5 bits (75), Expect = 0.023
Identities = 16/58 (27%), Positives = 30/58 (51%), Gaps = 4/58 (6%)
Frame = +3
Query: 11 SNRTNLASCLVATVFLIFILIIIFTL----YFTLFKPQDPKISVTAVQLPSFNLTNNS 64
+ R CL + L+FI +I+ + ++ +F+P+ P S+ V + NLT+ S
Sbjct: 273 TRRCGFCCCLCWLIGLLFIFLILLGIAAGVFYLVFRPKAPNYSIQTVSVKGMNLTSPS 446
>BP028899
Length = 474
Score = 33.1 bits (74), Expect = 0.030
Identities = 32/104 (30%), Positives = 46/104 (43%), Gaps = 4/104 (3%)
Frame = -3
Query: 88 YDSSFQLLSY-GKQIGFMFVPAGKINARRTQFMAATFTVQSLPLNLEPE-GLQMGPTV-E 144
Y +S Y G IG PAGK ARRT + T V + L P + + V
Sbjct: 472 YSNSTTTFFYDGVAIGEGVTPAGKAKARRTMRLNVTSVVVTKKLVGIPNWAIDIRDDVFN 293
Query: 145 IESTIEMVGRVRVLHLFSHHVEAKADCRVAIAVSDGSVL-GFHC 187
I S + G+V+VL +F+ V + +C + G + G HC
Sbjct: 292 ISSYTRIDGKVKVLGMFNRKVVVEMNCTSQYHRNPGLITRGNHC 161
>AV409132
Length = 405
Score = 28.1 bits (61), Expect = 0.97
Identities = 24/84 (28%), Positives = 38/84 (44%), Gaps = 12/84 (14%)
Frame = +1
Query: 16 LASCLVATVFLIFILIIIFTLYFTLFKPQDPKISVTAVQLPSFN----------LTNNST 65
+ + +VA +FL IL+IIF ++ L +P P+ + + +FN LT
Sbjct: 145 ILAAVVAFIFL--ILLIIFLIWIIL-RPTKPRFMLQDATVFAFNLSTQQHTPPLLTPTPN 315
Query: 66 TVNFTFSQYTSVKNPNR--GTFSH 87
T+ T S NPN G + H
Sbjct: 316 TLTLTLQVTLSSHNPNARIGVYYH 387
>TC8982 similar to UP|Q84QW4 (Q84QW4) OJ1191_A10.2 protein, partial (10%)
Length = 901
Score = 26.9 bits (58), Expect = 2.2
Identities = 15/55 (27%), Positives = 28/55 (50%), Gaps = 7/55 (12%)
Frame = +2
Query: 29 ILIIIFTLYFTLFKPQDPKISVTAVQLPS-------FNLTNNSTTVNFTFSQYTS 76
I I +++ +LF P I+ +LPS NL++ T++FT+++ S
Sbjct: 20 IFAISISIFISLFSPTHSAINCATQKLPSGRNYASCTNLSSLGATLHFTYNETNS 184
>TC19441
Length = 502
Score = 26.9 bits (58), Expect = 2.2
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 5/42 (11%)
Frame = +3
Query: 150 EMVGRVRVLHLFSHHVEAKADCRV-----AIAVSDGSVLGFH 186
EM+ V +LH +HHV +C++ S GSVLG H
Sbjct: 159 EMLSEVNLLHELAHHV--SPECKMMHI*NQCFSSSGSVLGSH 278
>CN825801
Length = 476
Score = 26.6 bits (57), Expect = 2.8
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -1
Query: 55 LPSFNLTNNSTTVNFTFSQYTSVKNPNRGTFSHY 88
L S + + NS + F Q ++NP GTF HY
Sbjct: 125 LNSHSYSPNSRSA*FHVFQLQLLRNPTPGTFPHY 24
>BP078290
Length = 411
Score = 26.2 bits (56), Expect = 3.7
Identities = 18/40 (45%), Positives = 23/40 (57%)
Frame = +2
Query: 57 SFNLTNNSTTVNFTFSQYTSVKNPNRGTFSHYDSSFQLLS 96
SFNL NNS +NF+F Q T SH DS+F +L+
Sbjct: 293 SFNLCNNS--INFSFLQLTQTM-----LLSH-DSNFNVLA 388
>TC14635 similar to GB|AAO44074.1|28466931|BT004808 At5g11090 {Arabidopsis
thaliana;}, partial (71%)
Length = 1048
Score = 25.8 bits (55), Expect = 4.8
Identities = 14/28 (50%), Positives = 16/28 (57%)
Frame = -3
Query: 156 RVLHLFSHHVEAKADCRVAIAVSDGSVL 183
RVLH S HVEA R+ +AV VL
Sbjct: 575 RVLHRRSTHVEAIGRTRIGVAVVVSGVL 492
>BP043338
Length = 463
Score = 25.4 bits (54), Expect = 6.3
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +2
Query: 135 EGLQMGPTVEIESTIEMVGRVRVLHLFSHHVEAK 168
EGLQ+ ++ + + GR+ +L LF H EA+
Sbjct: 239 EGLQLQLQLQPILDVPIYGRIAILQLFRPHGEAQ 340
>BP061066
Length = 250
Score = 25.4 bits (54), Expect = 6.3
Identities = 13/38 (34%), Positives = 23/38 (60%), Gaps = 5/38 (13%)
Frame = +1
Query: 59 NLTNNSTTVNFTFSQYTSVKN-----PNRGTFSHYDSS 91
N NN TT + T Y+++++ PN+ TFS+ ++S
Sbjct: 46 NWINNLTTDHRTCKSYSTIQSLEISTPNKTTFSYRETS 159
>TC19681 similar to AAQ56809 (AAQ56809) At4g12700, partial (18%)
Length = 645
Score = 25.4 bits (54), Expect = 6.3
Identities = 20/85 (23%), Positives = 42/85 (48%), Gaps = 2/85 (2%)
Frame = +3
Query: 10 PSNRTNLASCLVATV-FLIFIL-IIIFTLYFTLFKPQDPKISVTAVQLPSFNLTNNSTTV 67
P N ++ V F +F++ +++FT+ ++P+DP + + ++ +F LT+ S
Sbjct: 330 PENLGQNTMAMIGNVCFSVFVVGVLVFTIMAATYEPEDPLFN-PSTKISTF-LTSKS--- 494
Query: 68 NFTFSQYTSVKNPNRGTFSHYDSSF 92
N TF SV + +++F
Sbjct: 495 NATFKSDDSVVRTGEDFMAANETAF 569
>BI417934
Length = 502
Score = 25.4 bits (54), Expect = 6.3
Identities = 14/45 (31%), Positives = 28/45 (62%), Gaps = 1/45 (2%)
Frame = -3
Query: 35 TLYFTLFKPQDPKISVTAVQLPSFNLTNNSTT-VNFTFSQYTSVK 78
T++++L ++ KIS T + LPSF + T V+ +FS ++ ++
Sbjct: 242 TIWYSLIGMENKKISSTDLILPSFTKRPSLVTGVHSSFSPFSPLR 108
>NP840539 hypothetical protein [Lotus corniculatus var. japonicus]
Length = 3654
Score = 25.4 bits (54), Expect = 6.3
Identities = 12/42 (28%), Positives = 22/42 (51%)
Frame = -1
Query: 25 FLIFILIIIFTLYFTLFKPQDPKISVTAVQLPSFNLTNNSTT 66
F + +++FT+Y +P DP + + L F + NN+ T
Sbjct: 3324 FPVLACVLLFTIYAAGMEPTDPNL----IFLQQFLVLNNNGT 3211
>NP566647 unnamed protein product [Lotus japonicus]
Length = 2205
Score = 25.4 bits (54), Expect = 6.3
Identities = 12/42 (28%), Positives = 22/42 (51%)
Frame = -1
Query: 25 FLIFILIIIFTLYFTLFKPQDPKISVTAVQLPSFNLTNNSTT 66
F + +++FT+Y +P DP + + L F + NN+ T
Sbjct: 1872 FPVLACVLLFTIYAAGMEPTDPNL----IFLQQFLVLNNNGT 1759
>BP043776
Length = 532
Score = 25.4 bits (54), Expect = 6.3
Identities = 13/23 (56%), Positives = 16/23 (69%)
Frame = -1
Query: 90 SSFQLLSYGKQIGFMFVPAGKIN 112
SSF LLS+G + F F P GK+N
Sbjct: 97 SSF-LLSFGYYVYFSFGPCGKLN 32
>TC14760 weakly similar to UP|Q8GUU3 (Q8GUU3) FEG protein, partial (8%)
Length = 1001
Score = 25.0 bits (53), Expect = 8.2
Identities = 13/30 (43%), Positives = 18/30 (59%), Gaps = 1/30 (3%)
Frame = -2
Query: 4 HEHETPPSNRTNLASCLVATVFL-IFILII 32
H H++P +SC V TV L IF+L+I
Sbjct: 661 HSHQSPNKKNDPNSSCYV*TVSLSIFVLLI 572
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.324 0.136 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,118,902
Number of Sequences: 28460
Number of extensions: 37328
Number of successful extensions: 358
Number of sequences better than 10.0: 40
Number of HSP's better than 10.0 without gapping: 355
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 357
length of query: 187
length of database: 4,897,600
effective HSP length: 85
effective length of query: 102
effective length of database: 2,478,500
effective search space: 252807000
effective search space used: 252807000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)
Medicago: description of AC142526.4