BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC142394.3 - phase: 0
(321 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP080173 64 4e-11
BP036240 63 6e-11
BP056414 63 8e-11
TC19596 62 2e-10
TC13560 59 1e-09
TC18883 52 1e-07
TC11323 50 5e-07
BP043532 48 2e-06
BP062688 46 7e-06
AV780445 36 1e-05
BP069214 45 2e-05
TC11605 43 8e-05
BP053577 42 1e-04
TC10933 similar to UP|Q9LNS0 (Q9LNS0) F1L3.4, partial (6%) 41 2e-04
BP052637 39 0.001
TC19127 39 0.002
AV428268 37 0.006
BP041113 35 0.022
BP085925 35 0.022
BP068806 33 0.084
>BP080173
Length = 382
Score = 63.5 bits (153), Expect = 4e-11
Identities = 37/108 (34%), Positives = 54/108 (49%), Gaps = 1/108 (0%)
Frame = -2
Query: 176 VGWKEPAIRWVKINTDGACKDGS-IAGCGGLIRGSEGEWLAGFSKFLGKCDAFIAELWGV 234
V W P W+KIN DG+ S A CGG++ EG ++ G S LG AELWG+
Sbjct: 351 VRWPVPMEGWIKINIDGSFAPSSNCAACGGVMWDHEGTFITGASVRLGCYFINHAELWGI 172
Query: 235 LEGLRCAKRMGFTAVELNVDSLVVVNIITSERESNASGRSLVQKIRKL 282
G + A+ GF + + DS VV+++ + LV+ I+ L
Sbjct: 171 FHGAKIAQARGFKKILVESDSSFVVSLVNQGCSPSKPATPLVKDIKSL 28
>BP036240
Length = 567
Score = 63.2 bits (152), Expect = 6e-11
Identities = 35/125 (28%), Positives = 59/125 (47%), Gaps = 3/125 (2%)
Frame = -3
Query: 178 WKEPAIRWVKINTDGACKDGSIAGCGGLIRGSEGEWLAGFSKFLGKC---DAFIAELWGV 234
W P W K + DGA +A CGG++R S W+ GF + +G + F+ EL +
Sbjct: 562 WTAPDEGWTKFDVDGALSRDLLASCGGVLRDSRRHWIKGFCRNMGDLTYHNVFLVELSAI 383
Query: 235 LEGLRCAKRMGFTAVELNVDSLVVVNIITSERESNASGRSLVQKIRKLLQMEWKVKVKHS 294
+ A M V + D+L +++++SE SL I ++ + + +H+
Sbjct: 382 QSAVEIALSMDLQHVIVESDALEAIHLLSSENVHAHPFGSLAASILRMQRDHGSIVFQHT 203
Query: 295 YREAN 299
REAN
Sbjct: 202 PREAN 188
>BP056414
Length = 606
Score = 62.8 bits (151), Expect = 8e-11
Identities = 40/136 (29%), Positives = 61/136 (44%), Gaps = 4/136 (2%)
Frame = -3
Query: 178 WKEPAIRWVKINTDGACKDGSIAGCGGLIRGSEGEWLAGFSKFLGKC---DAFIAELWGV 234
W P W K+N DGA A CGG++R + G W GF++ LG AF EL V
Sbjct: 562 WTRPPEGWFKVNVDGALSGDLQASCGGVVRDAAGSWSKGFARSLGVLRWHXAFFVELMAV 383
Query: 235 LEGLRCAKRMGFTAVELNVDSLVVVNIITSERESNA-SGRSLVQKIRKLLQMEWKVKVKH 293
+ V + DS VV ++ + S+ + I++ + + + +
Sbjct: 382 QTAVDFIMSWDIPQVIIESDSQQVVELLQNFNASSPHQYGEIAWDIQQKISVHGSIVITS 203
Query: 294 SYREANRCADALANIG 309
+ REAN AD LA +G
Sbjct: 202 TPREANFLADYLAKVG 155
>TC19596
Length = 499
Score = 61.6 bits (148), Expect = 2e-10
Identities = 40/124 (32%), Positives = 61/124 (48%), Gaps = 1/124 (0%)
Frame = +1
Query: 187 KINTDGACK-DGSIAGCGGLIRGSEGEWLAGFSKFLGKCDAFIAELWGVLEGLRCAKRMG 245
++++DGA K D G GG++R + G W++GF DA AE+ + GL
Sbjct: 4 RMDSDGAFKHDDDRMGMGGIVRDAHGAWISGFYAGSLGGDALRAEIAALKHGLTLLWNAH 183
Query: 246 FTAVELNVDSLVVVNIITSERESNASGRSLVQKIRKLLQMEWKVKVKHSYREANRCADAL 305
VD L +V + ++R + S + IR LL +W V + + REAN AD L
Sbjct: 184 VRRATCEVDCLDIVEALENDRYQFHALASELLDIRLLLDRDWTVTLAYVPREANAAADCL 363
Query: 306 ANIG 309
A +G
Sbjct: 364 AGLG 375
>TC13560
Length = 571
Score = 58.5 bits (140), Expect = 1e-09
Identities = 32/90 (35%), Positives = 45/90 (49%), Gaps = 3/90 (3%)
Frame = +2
Query: 178 WKEPAIRWVKINTDGACKDGSIAGCGGLIRGSEGEWLAGFSKFLG---KCDAFIAELWGV 234
W +P WVKIN DGA + A C G++R ++G WL GF G + F+ EL V
Sbjct: 146 WIKPDAGWVKINVDGAVSNCLQASCRGVLRNADGSWLKGFRWNFGTFSSANVFLTELMAV 325
Query: 235 LEGLRCAKRMGFTAVELNVDSLVVVNIITS 264
+ A + V + DSL VV+ + S
Sbjct: 326 KTAVEAAMSLDLARVIVESDSLEVVDFLHS 415
>TC18883
Length = 821
Score = 52.4 bits (124), Expect = 1e-07
Identities = 39/127 (30%), Positives = 62/127 (48%), Gaps = 6/127 (4%)
Frame = +1
Query: 201 GCGGLIRGSEGEWLAGFSKFLGKCDAFIAELWGVLEGLRCAKRMGFTAVELNVDSLVVVN 260
G GG+ R S+ E L FSK +G+ AF AE+W + E L + + DS + V
Sbjct: 10 GIGGIFRNSDNEILGFFSKHVGEGFAFEAEVWAIFEALTICVNRQLRNLTIESDSSLAVG 189
Query: 261 IITSERESNASGRSLVQKIRKLLQME-WK-----VKVKHSYREANRCADALANIGCIMGN 314
+ +N S R + + KL Q++ W + VKH +REAN D LA G
Sbjct: 190 WV-----NNRSXRPW-KLLNKLNQIDLWVAEVNCLSVKHIFREANGEVDKLAKRGADHPR 351
Query: 315 EMMFYES 321
++ ++++
Sbjct: 352 DLYYFKT 372
>TC11323
Length = 657
Score = 50.1 bits (118), Expect = 5e-07
Identities = 30/88 (34%), Positives = 43/88 (48%), Gaps = 1/88 (1%)
Frame = +2
Query: 178 WKEPAIRWVKINTDGACKDG-SIAGCGGLIRGSEGEWLAGFSKFLGKCDAFIAELWGVLE 236
W +P K+N DG+ AG GGL+R ++ GFS FL L +LE
Sbjct: 5 WLKPVHGRFKLNFDGSSLGNRGNAGGGGLLRDGSSNFIFGFSIFLAVAQIMKLSLCAILE 184
Query: 237 GLRCAKRMGFTAVELNVDSLVVVNIITS 264
GL K +G+ + + DS +VV+ I S
Sbjct: 185 GLLVCKPLGYDGIGIECDSNIVVSWIRS 268
>BP043532
Length = 465
Score = 48.1 bits (113), Expect = 2e-06
Identities = 28/89 (31%), Positives = 41/89 (45%)
Frame = -2
Query: 221 LGKCDAFIAELWGVLEGLRCAKRMGFTAVELNVDSLVVVNIITSERESNASGRSLVQKIR 280
+G C AELWG++ GLR A GF+ + + DS + + LV++
Sbjct: 464 VGVCSILHAELWGLVHGLRFALGRGFSKILIEADSAGTIEFLNKGCPVVHPCFPLVREFH 285
Query: 281 KLLQMEWKVKVKHSYREANRCADALANIG 309
L+ + KH YRE N AD+ A G
Sbjct: 284 HLVGQNCYIHWKHVYREVNFVADSFAKNG 198
>BP062688
Length = 536
Score = 46.2 bits (108), Expect = 7e-06
Identities = 26/74 (35%), Positives = 39/74 (52%), Gaps = 1/74 (1%)
Frame = -1
Query: 236 EGLRCAKRMGFTAVELNVDSLVVVNIITSERESNASG-RSLVQKIRKLLQMEWKVKVKHS 294
+GL +GF V VD L + ++TS R+ R +++ IR +L W V + H
Sbjct: 383 KGLLHTWELGFLHVVCYVDCLELQEVLTSTRDVQTYWHRDVIEMIRAVLARSWNVTINHV 204
Query: 295 YREANRCADALANI 308
R+ N ADALAN+
Sbjct: 203 TRDGNAVADALANL 162
>AV780445
Length = 504
Score = 36.2 bits (82), Expect(2) = 1e-05
Identities = 24/90 (26%), Positives = 36/90 (39%), Gaps = 1/90 (1%)
Frame = +1
Query: 126 IWNWRNKETHNDNYQRPLHAKNIIMIYVNDYHNAIAKFVIVTSQPRHLEEVGWKEPAIRW 185
+W WRN D+ P +++ I P W+ P
Sbjct: 244 VWKWRNNMVFEDS---PWSVDEAWRKLGHEHDEFITFSDGDAGDPGSWLSSRWQPPRQGT 414
Query: 186 VKINTDGACKDGS-IAGCGGLIRGSEGEWL 214
VK+N DG+ ++ G GGLIR +G+WL
Sbjct: 415 VKLNVDGSGREQDRCMGAGGLIRDDQGKWL 504
Score = 28.5 bits (62), Expect(2) = 1e-05
Identities = 15/34 (44%), Positives = 19/34 (55%)
Frame = +2
Query: 66 HVLRDCPKATALWLCVVDNAARTSFFEGDLSSWI 99
H LRDCP + +WL + A T+ GD SWI
Sbjct: 95 HCLRDCPHSQEIWL-RLGAWAWTNLGVGDHRSWI 193
>BP069214
Length = 545
Score = 44.7 bits (104), Expect = 2e-05
Identities = 26/88 (29%), Positives = 38/88 (42%)
Frame = -2
Query: 230 ELWGVLEGLRCAKRMGFTAVELNVDSLVVVNIITSERESNASGRSLVQKIRKLLQMEWKV 289
EL + GL GF V DS + ++ +L+ I+ LL EW+V
Sbjct: 394 ELLAMWHGLDLVWDRGFRQVICQSDSTEALALVLGVPPPRHRYAALIWSIKDLLSREWEV 215
Query: 290 KVKHSYREANRCADALANIGCIMGNEMM 317
+ H RE N CAD LA G + ++
Sbjct: 214 SMTHMLREGNSCADFLAKYGAMQDEPLV 131
>TC11605
Length = 546
Score = 42.7 bits (99), Expect = 8e-05
Identities = 24/66 (36%), Positives = 36/66 (54%), Gaps = 1/66 (1%)
Frame = +3
Query: 178 WKEPAIRWVKINTDGACKDG-SIAGCGGLIRGSEGEWLAGFSKFLGKCDAFIAELWGVLE 236
W P +K N DG+ + I+G GG++R +E L FSK LG A+ AE+ +L
Sbjct: 348 WCPPEANILKFNVDGSARGSPGISGAGGILRNAESAVLGRFSKPLGVLCAYQAEVKAILL 527
Query: 237 GLRCAK 242
L+ +K
Sbjct: 528 ALKFSK 545
>BP053577
Length = 463
Score = 42.4 bits (98), Expect = 1e-04
Identities = 33/111 (29%), Positives = 49/111 (43%), Gaps = 1/111 (0%)
Frame = +3
Query: 197 GSIAGCGGLIRGSEGEWLA-GFSKFLGKCDAFIAELWGVLEGLRCAKRMGFTAVELNVDS 255
G IAG G + R +GE +A S + +AE G+ ++ A +GF + L D
Sbjct: 3 GGIAGLGMVARNCDGEVMASACSSPVSISSPLLAEALGLRWTMQLATDLGFRRITLETDC 182
Query: 256 LVVVNIITSERESNASGRSLVQKIRKLLQMEWKVKVKHSYREANRCADALA 306
L + N S+ E + S++ R L+ V V R N AD LA
Sbjct: 183 LQLFNTWRSQEEGRSYLSSIISDCRILISAFDYVSVSFVRRTGNTVADFLA 335
>TC10933 similar to UP|Q9LNS0 (Q9LNS0) F1L3.4, partial (6%)
Length = 596
Score = 41.2 bits (95), Expect = 2e-04
Identities = 24/74 (32%), Positives = 31/74 (41%), Gaps = 29/74 (39%)
Frame = +2
Query: 178 WKEPAIRWVKINTDGACKDG-SIAGCGG----------------------------LIRG 208
W P++ WVK+NTDG+ G S+ GCGG L++
Sbjct: 107 WTPPSVGWVKLNTDGSFTRGSSLMGCGGLC*K**SDLFM*P*HNNFSF*DNWLFDSLLQD 286
Query: 209 SEGEWLAGFSKFLG 222
G W AGFS F G
Sbjct: 287 RHGNWRAGFSSFEG 328
>BP052637
Length = 473
Score = 38.9 bits (89), Expect = 0.001
Identities = 22/64 (34%), Positives = 33/64 (51%), Gaps = 12/64 (18%)
Frame = -2
Query: 188 INTDGACKDGSIA-----GCG-------GLIRGSEGEWLAGFSKFLGKCDAFIAELWGVL 235
INTD C+ G + GCG G++R W++GF+ G DAF+A+L +
Sbjct: 451 INTDRGCETGRVMR*WQHGCGLKCYWGSGVLRDKVXRWVSGFAMAFGTGDAFMAKLQALH 272
Query: 236 EGLR 239
E L+
Sbjct: 271 ESLQ 260
>TC19127
Length = 588
Score = 38.5 bits (88), Expect = 0.002
Identities = 22/58 (37%), Positives = 33/58 (55%), Gaps = 1/58 (1%)
Frame = +1
Query: 203 GGLIRGSEGEWLAGFSKFL-GKCDAFIAELWGVLEGLRCAKRMGFTAVELNVDSLVVV 259
G +IR EG +A +K + D+ +AE GV L +++GFT + L DSLV+V
Sbjct: 67 GAIIRNKEGLTMAAATKKA*AQGDSIMAEAMGVQWALEWTRQLGFTQISLETDSLVLV 240
>AV428268
Length = 429
Score = 36.6 bits (83), Expect = 0.006
Identities = 29/126 (23%), Positives = 48/126 (38%), Gaps = 1/126 (0%)
Frame = +3
Query: 7 NDAQDEIWLKLWNIRAPERIKHFMSMLYHGRLSTNLRKHKMGIG-NPMCRFCHDEIESEI 65
N D I+ LW+++ P + R+ T + ++ G+ + +C C + ES
Sbjct: 48 NAQWDNIFKLLWSVKVPSNAIALSWKVLINRVQTKVNLNRRGVVISNVCPLCSLDEESTD 227
Query: 66 HVLRDCPKATALWLCVVDNAARTSFFEGDLSSWIKFNLFTNVYWNNNVEWRDFWATACHT 125
H+L CP +W + + S F D +L + N W A
Sbjct: 228 HLLFSCPIV*RIWSKISE*FGVFSVFPNDSHGHFLQHLGSCGSLNFRKRGWFVWIAAVVC 407
Query: 126 IWNWRN 131
IW RN
Sbjct: 408 IWQGRN 425
>BP041113
Length = 503
Score = 34.7 bits (78), Expect = 0.022
Identities = 23/62 (37%), Positives = 32/62 (51%), Gaps = 4/62 (6%)
Frame = +1
Query: 11 DEIWLKLWNIRAPERIKHFMSMLYHGRLST--NLRKHKMGIGNPM--CRFCHDEIESEIH 66
D I+ +W AP +K F GRL T NL K ++ + M C+FC E+ES H
Sbjct: 313 DPIFATIWRTVAPS-VKAFAWRCLLGRLPTYDNLIKRQVVVDPAMTVCKFCQGEVESVTH 489
Query: 67 VL 68
+L
Sbjct: 490 LL 495
>BP085925
Length = 334
Score = 34.7 bits (78), Expect = 0.022
Identities = 16/36 (44%), Positives = 20/36 (55%)
Frame = -2
Query: 274 SLVQKIRKLLQMEWKVKVKHSYREANRCADALANIG 309
SLV I+ L+ W V + H+ RE N CAD L G
Sbjct: 297 SLVWDIQDLMSRSWHVVLNHTLREGNACADFLTKHG 190
>BP068806
Length = 418
Score = 32.7 bits (73), Expect = 0.084
Identities = 19/79 (24%), Positives = 37/79 (46%), Gaps = 1/79 (1%)
Frame = +3
Query: 23 PERIKHFMSMLYHGRLSTNLRKHKMGIG-NPMCRFCHDEIESEIHVLRDCPKATALWLCV 81
PE+I+ ++ + G + N + + + +P C + E H LRDC A +W+
Sbjct: 153 PEKIRVWVWFIMQGAIQVNSFRFRCKLATSPAGPRCQHQFEDIWHCLRDCRIARDVWV-R 329
Query: 82 VDNAARTSFFEGDLSSWIK 100
++ FF + +W+K
Sbjct: 330 LNALGWPGFFVSNAQAWVK 386
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.137 0.450
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,397,066
Number of Sequences: 28460
Number of extensions: 123864
Number of successful extensions: 604
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 597
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 599
length of query: 321
length of database: 4,897,600
effective HSP length: 90
effective length of query: 231
effective length of database: 2,336,200
effective search space: 539662200
effective search space used: 539662200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 55 (25.8 bits)
Medicago: description of AC142394.3