BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC142394.1 - phase: 0 /pseudo
(426 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV409220 64 5e-11
TC14630 32 0.26
TC9538 similar to UP|RLK5_ARATH (P47735) Receptor-like protein k... 30 0.99
TC14460 29 1.7
AW719473 28 2.2
CB828319 27 4.9
TC10151 similar to UP|HISX_BRAOC (P24226) Histidinol dehydrogena... 27 6.4
AV427815 27 8.4
TC9924 UP|RBL_LOTJA (Q9BBU1) Ribulose bisphosphate carboxylase l... 27 8.4
TC7964 homologue to UP|O81926 (O81926) Thaumatin-like protein PR... 27 8.4
>AV409220
Length = 406
Score = 63.9 bits (154), Expect = 5e-11
Identities = 43/133 (32%), Positives = 72/133 (53%), Gaps = 1/133 (0%)
Frame = +2
Query: 62 LYEVALAFRLLRQGGYYVNAELFDCLKCSKKSLRVKY-GEDVKGLIALYEASQLSIEGED 120
L+E AL+FRLLR+ GY+ +A++F + G+DVKG+++LYEAS LS EGE
Sbjct: 8 LHETALSFRLLREYGYHASADIFMSFMNLDGNFEANLIGKDVKGMLSLYEASFLSYEGEQ 187
Query: 121 GLNDLGYLSCELLQAWLPRHQDHIQAIYVSNTLQYPIHYGLSRFMDKSIFINDLKAKNKW 180
L++ + L++ + + V++ L+ P+H + R + + K KN
Sbjct: 188 ILDEAKTFTILHLKSAMSDDSSNYLLEQVNHALELPLHRRVQRLEARWYIDSYAKRKNAN 367
Query: 181 ICLDELAKMNSSI 193
L E AK++ +I
Sbjct: 368 KVLLEAAKLDFNI 406
>TC14630
Length = 617
Score = 31.6 bits (70), Expect = 0.26
Identities = 13/28 (46%), Positives = 20/28 (71%)
Frame = -2
Query: 33 YFVEEIKVALEKQFLILSSNPIDFVSSH 60
+F ++V LEK +++LSSNP F S+H
Sbjct: 532 FFCLNVEVQLEKIYILLSSNPFCFCSTH 449
>TC9538 similar to UP|RLK5_ARATH (P47735) Receptor-like protein kinase 5
precursor , partial (10%)
Length = 1067
Score = 29.6 bits (65), Expect = 0.99
Identities = 17/58 (29%), Positives = 29/58 (49%), Gaps = 1/58 (1%)
Frame = -2
Query: 175 KAKNK-WICLDELAKMNSSIVKFMNKNESVEVFKWWEDLGLAKEMKFAGYNPLKWYMW 231
+A+ K W+C L K NSS + F+++N KW + L + +G +W +W
Sbjct: 1045 RAREKAWLCTTNLPKPNSSTIHFLHENG----LKWLDSL----TIPISGILMGQWSLW 896
>TC14460
Length = 1520
Score = 28.9 bits (63), Expect = 1.7
Identities = 10/37 (27%), Positives = 19/37 (51%)
Frame = +1
Query: 205 VFKWWEDLGLAKEMKFAGYNPLKWYMWPMACFTDPCF 241
++ W LG+ ++ F ++PL + W CF C+
Sbjct: 541 LYLWPATLGVCVDIFFCKFHPLVCFCWSPYCFMGTCY 651
>AW719473
Length = 555
Score = 28.5 bits (62), Expect = 2.2
Identities = 12/20 (60%), Positives = 15/20 (75%)
Frame = +1
Query: 125 LGYLSCELLQAWLPRHQDHI 144
L +LS LL +WLP+HQ HI
Sbjct: 154 LRFLSHPLLPSWLPKHQLHI 213
>CB828319
Length = 551
Score = 27.3 bits (59), Expect = 4.9
Identities = 24/97 (24%), Positives = 45/97 (45%), Gaps = 1/97 (1%)
Frame = +1
Query: 185 ELAKMNSSIVKFMNKNESVEVFKWWEDLGLAKEMKFAGYNPLKWYMWPMACFTDPCFSNE 244
ELAK++ + + +++ E E+ KW+ + L E+ + + L Y A +P +
Sbjct: 118 ELAKLDYNSCQALHQTEWGEIQKWYSESRLG-ELGLSRRSLLLAYFLAAASIFEPERYHV 294
Query: 245 RVELTKPISLVYIIDD-IFDVHGTLDQLTLFTEAVNR 280
R+ K SL+ I D D + FT+ +N+
Sbjct: 295 RLAWAKTTSLLETIASYATDAEMRKDFMERFTDCINK 405
>TC10151 similar to UP|HISX_BRAOC (P24226) Histidinol dehydrogenase,
chloroplast precursor (HDH) , partial (20%)
Length = 534
Score = 26.9 bits (58), Expect = 6.4
Identities = 14/42 (33%), Positives = 22/42 (52%), Gaps = 3/42 (7%)
Frame = +2
Query: 162 SRFMDKSIFIN---DLKAKNKWICLDELAKMNSSIVKFMNKN 200
S M++SI + + A N W+CL + +N SI+ N N
Sbjct: 293 SEMMNQSIVLYIFCKMLALNYWLCLCSICNLNVSILHLCNHN 418
>AV427815
Length = 421
Score = 26.6 bits (57), Expect = 8.4
Identities = 11/19 (57%), Positives = 15/19 (78%)
Frame = +1
Query: 337 EAHWLNSGILPTTEEYLNN 355
E+HWLN +LPTT YL++
Sbjct: 118 ESHWLNLSLLPTT-NYLSH 171
>TC9924 UP|RBL_LOTJA (Q9BBU1) Ribulose bisphosphate carboxylase large chain
precursor (RuBisCO large subunit) , complete
Length = 1592
Score = 26.6 bits (57), Expect = 8.4
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = +3
Query: 303 VTNNFAEMVYKKHGFNPIDTLKISWVRLLNAFLK 336
VTN F +V GF + L++ +R+ NA++K
Sbjct: 501 VTNMFTSIVGNVFGFKALRALRLEDLRIPNAYVK 602
>TC7964 homologue to UP|O81926 (O81926) Thaumatin-like protein PR-5b
precursor (Fragment), partial (74%)
Length = 778
Score = 26.6 bits (57), Expect = 8.4
Identities = 18/51 (35%), Positives = 27/51 (52%), Gaps = 1/51 (1%)
Frame = -3
Query: 293 MKVSLSSLYKVTNNFA-EMVYKKHGFNPIDTLKISWVRLLNAFLKEAHWLN 342
+++ ++L KV F +V K HG P T++ISW + FL HW N
Sbjct: 695 LELDPAALCKVVVGFILRVVVKNHGTCP*RTVRISWA---HRFL--VHWYN 558
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.139 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,624,569
Number of Sequences: 28460
Number of extensions: 104115
Number of successful extensions: 470
Number of sequences better than 10.0: 20
Number of HSP's better than 10.0 without gapping: 469
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 469
length of query: 426
length of database: 4,897,600
effective HSP length: 93
effective length of query: 333
effective length of database: 2,250,820
effective search space: 749523060
effective search space used: 749523060
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC142394.1