BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC142224.5 - phase: 0 /pseudo
(625 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18710 93 1e-19
TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%) 56 2e-08
AV780705 55 3e-08
TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, p... 45 3e-05
TC12829 32 0.31
TC14793 similar to UP|Q96569 (Q96569) L-lactate dehydrogenase (... 32 0.40
TC12501 similar to UP|Q9LR83 (Q9LR83) F21B7.2, partial (55%) 30 0.89
TC8979 weakly similar to UP|Q8RY59 (Q8RY59) At1g32230/F3C3_1, pa... 28 3.4
BP067760 27 7.5
TC20173 similar to PIR|S20941|S20941 protochlorophyllide reducta... 27 7.5
TC14439 similar to UP|Q9FIC1 (Q9FIC1) Gb|AAF19567.1, partial (49%) 27 9.8
BP036975 27 9.8
TC7915 similar to UP|Q8VYW9 (Q8VYW9) Aminotransferase 1, complete 27 9.8
AV426226 27 9.8
TC10705 27 9.8
>TC18710
Length = 843
Score = 93.2 bits (230), Expect = 1e-19
Identities = 55/188 (29%), Positives = 92/188 (48%), Gaps = 14/188 (7%)
Frame = +2
Query: 249 VSEGSFPPNLIDTNIALIAKIDRPKSMKHLCPVSLCNVIYKILSKVLANRLKQILHKCIS 308
+ P + +T + LI K+ P+S+ P+S C +YK++SK+ RLK + IS
Sbjct: 272 LERAEIPNEINETLVTLIPKVPHPESINQFRPISGCTFLYKVISKIFVARLKDAMPDLIS 451
Query: 309 DSQAAFVPSRFILENALTYFEVLHYMKCKTKGKEGNIALKQDVSKAFDRIKWSYLQAVM- 367
Q+ F+ R I +N L E H + + +K D++KA+DR++W +L++ +
Sbjct: 452 PMQSGFIQGRQIQDNLLIVQEAFHAINRPGALGRNHSIIKLDMNKAYDRVEWKFLESSLL 631
Query: 368 ----EKKWDSQMFGLTGSCN--------V*P-IDPHCGIRQGGPFSPYLYIICSEGLNSY 414
W + L + V P + P G+RQG PFSPYL++ E L+
Sbjct: 632 AFGFSTNWVKMIMILVSGVSYNYKINGVVGPKLLPQRGLRQGDPFSPYLFLFTMEVLSLL 811
Query: 415 IKHHENTG 422
I++ N G
Sbjct: 812 IQNSFNMG 835
>TC18742 similar to UP|Q9FZG5 (Q9FZG5) T2E6.4, partial (3%)
Length = 912
Score = 56.2 bits (134), Expect = 2e-08
Identities = 36/121 (29%), Positives = 61/121 (49%)
Frame = -3
Query: 436 ITQLLFADDSFLFYKAYVSEDTILKNILYTYEAASGQAINYRKSSIAFSRNTDSIGHHNI 495
I+ L FAD+ +F Y+ I+ N+L+ ++ SG N KS + + + I
Sbjct: 373 ISHLCFADNLMVFSNGYLESIAIINNVLHIFQHLSGLTPNPAKSEVFILVKETT--KNQI 200
Query: 496 THLLGVVESMWHGKYLGLPSMVERDKKSIFSFIKERIWKNIQNWSAWSLSRAGKEVLLKS 555
T++LG E + LG+P + + K S + +R +Q+W+ SLS AG+ L+ S
Sbjct: 199 TNMLGYKEGKLPVR*LGVPQLTTKLKASDCKVLVDRKPSKLQHWTGRSLSYAGRLQLINS 20
Query: 556 I 556
I
Sbjct: 19 I 17
>AV780705
Length = 524
Score = 55.1 bits (131), Expect = 3e-08
Identities = 25/60 (41%), Positives = 41/60 (67%)
Frame = -2
Query: 508 GKYLGLPSMVERDKKSIFSFIKERIWKNIQNWSAWSLSRAGKEVLLKSIAQYIPTYSWAI 567
G YLGLP++ R+K +I+E++ + ++ W L++AGKEVL+K+I Q IP+Y+ I
Sbjct: 490 GHYLGLPAIWGRNKSHSLVWIEEKVKEKLEGWKETLLNQAGKEVLIKAIIQAIPSYAMTI 311
>TC18198 weakly similar to UP|Q9XGZ5 (Q9XGZ5) T1N24.20 protein, partial
(14%)
Length = 592
Score = 45.1 bits (105), Expect = 3e-05
Identities = 21/57 (36%), Positives = 34/57 (58%)
Frame = -3
Query: 252 GSFPPNLIDTNIALIAKIDRPKSMKHLCPVSLCNVIYKILSKVLANRLKQILHKCIS 308
G P L +T + LI KI ++++ P+S C+ IYK++SK++ RLK + IS
Sbjct: 179 GELPVELNETLVTLIPKIPHAEAIQQFRPISCCSFIYKVISKIIVARLKPDMVNLIS 9
>TC12829
Length = 448
Score = 32.0 bits (71), Expect = 0.31
Identities = 14/25 (56%), Positives = 19/25 (76%)
Frame = +3
Query: 544 LSRAGKEVLLKSIAQYIPTYSWAIF 568
L RAG+EVL+KS+ + IPTY + F
Sbjct: 261 LYRAGREVLIKSVTKAIPTYIMSCF 335
>TC14793 similar to UP|Q96569 (Q96569) L-lactate dehydrogenase (LDH) ,
partial (27%)
Length = 569
Score = 31.6 bits (70), Expect = 0.40
Identities = 12/37 (32%), Positives = 21/37 (56%)
Frame = -2
Query: 470 SGQAINYRKSSIAFSRNTDSIGHHNITHLLGVVESMW 506
SG+ N+R + I N D I +H++ ++ + SMW
Sbjct: 415 SGKQKNFRTNHIMMGENQDKIKYHSVPKVILIASSMW 305
>TC12501 similar to UP|Q9LR83 (Q9LR83) F21B7.2, partial (55%)
Length = 1062
Score = 30.4 bits (67), Expect = 0.89
Identities = 20/64 (31%), Positives = 27/64 (41%), Gaps = 7/64 (10%)
Frame = -1
Query: 490 IGHHNITHLLGVVESMWHGKYLGLPS-MVERDKKSIFSFI------KERIWKNIQNWSAW 542
+ H ++ + E WH K+ L M ER+ S F + ER W I S W
Sbjct: 456 VSHQELSPRISKCELPWHRKWCSLQHRMAEREFSSSFGTLAGSYNTSERAWFAIVLLSGW 277
Query: 543 SLSR 546
SL R
Sbjct: 276 SLPR 265
>TC8979 weakly similar to UP|Q8RY59 (Q8RY59) At1g32230/F3C3_1, partial
(12%)
Length = 833
Score = 28.5 bits (62), Expect = 3.4
Identities = 14/43 (32%), Positives = 19/43 (43%), Gaps = 10/43 (23%)
Frame = +2
Query: 390 HCGIRQGGPFSP----------YLYIICSEGLNSYIKHHENTG 422
HC + GGPFSP Y++ S+G + HH G
Sbjct: 620 HCNLSTGGPFSPECVLLDGACARTYLLLSDGAFLILLHHNFIG 748
>BP067760
Length = 535
Score = 27.3 bits (59), Expect = 7.5
Identities = 14/31 (45%), Positives = 20/31 (64%), Gaps = 3/31 (9%)
Frame = -1
Query: 116 LWLSCGVFGALVSHAYGNKNLLML---KHLV 143
+WLSCG++ SH YGN + +L KHL+
Sbjct: 421 VWLSCGIY----SHIYGNISRWILPSWKHLM 341
>TC20173 similar to PIR|S20941|S20941 protochlorophyllide reductase
precursor - garden pea {Pisum sativum;}
, partial (28%)
Length = 361
Score = 27.3 bits (59), Expect = 7.5
Identities = 10/24 (41%), Positives = 13/24 (53%)
Frame = -3
Query: 98 HLLCLPFLQLLMWKTVHDLWLSCG 121
H + LP L+ W + HD W CG
Sbjct: 284 HNIALPQSFLVFWGSSHDSWCCCG 213
>TC14439 similar to UP|Q9FIC1 (Q9FIC1) Gb|AAF19567.1, partial (49%)
Length = 2120
Score = 26.9 bits (58), Expect = 9.8
Identities = 11/38 (28%), Positives = 23/38 (59%)
Frame = -1
Query: 510 YLGLPSMVERDKKSIFSFIKERIWKNIQNWSAWSLSRA 547
+ +P++ D+KS S + R W N Q+ S+++ +R+
Sbjct: 371 HTSIPAVFNTDRKSSKSISEARAWNNQQSTSSFTKNRS 258
>BP036975
Length = 528
Score = 26.9 bits (58), Expect = 9.8
Identities = 24/87 (27%), Positives = 41/87 (46%), Gaps = 6/87 (6%)
Frame = +1
Query: 265 LIAKIDRPKSMKHLCPVSLCNVIYKILSKVLANRLK------QILHKCISDSQAAFVPSR 318
L+ ++ RP S L +S C +K KVL ++ +K +S+ +A +PS+
Sbjct: 127 LMTQLPRPTSCNRL--ISAC--FFKSADKVLEQNIRISPKTLAFSNKNLSN*MSASLPSK 294
Query: 319 FILENALTYFEVLHYMKCKTKGKEGNI 345
F +EN L + K +G E N+
Sbjct: 295 FEIENNLRLY-------LKQQGTEFNL 354
>TC7915 similar to UP|Q8VYW9 (Q8VYW9) Aminotransferase 1, complete
Length = 1622
Score = 26.9 bits (58), Expect = 9.8
Identities = 9/29 (31%), Positives = 18/29 (62%)
Frame = +1
Query: 516 MVERDKKSIFSFIKERIWKNIQNWSAWSL 544
++E + + S K+ W++ QNW++W L
Sbjct: 1117 VMEEIQYELRSGTKQGCWESFQNWTSWKL 1203
>AV426226
Length = 420
Score = 26.9 bits (58), Expect = 9.8
Identities = 12/24 (50%), Positives = 14/24 (58%)
Frame = +3
Query: 98 HLLCLPFLQLLMWKTVHDLWLSCG 121
H L L L +L+W V DL L CG
Sbjct: 240 HFLTLSLLFILLWCMVPDLGLHCG 311
>TC10705
Length = 503
Score = 26.9 bits (58), Expect = 9.8
Identities = 15/36 (41%), Positives = 21/36 (57%)
Frame = +3
Query: 40 GSTCKVMEFNVHQLVLPVIMIWKMRCICLCTGSLLL 75
G+ CK + Q+ L +I+IWK +C TGS LL
Sbjct: 342 GT*CKY----IIQVCLCIIIIWKGNPLCKFTGSALL 437
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.342 0.149 0.502
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,987,552
Number of Sequences: 28460
Number of extensions: 215101
Number of successful extensions: 1727
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 1710
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1724
length of query: 625
length of database: 4,897,600
effective HSP length: 96
effective length of query: 529
effective length of database: 2,165,440
effective search space: 1145517760
effective search space used: 1145517760
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC142224.5