BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC142224.1 - phase: 0
(309 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15328 weakly similar to UP|Q9LP71 (Q9LP71) T1N15.15, partial (... 495 e-141
TC11354 similar to UP|CAE54480 (CAE54480) Alpha glucosidase II ... 37 0.004
TC16065 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich... 36 0.009
BP069129 35 0.016
AU240151 35 0.016
AW719960 35 0.016
TC19891 homologue to PIR|S14182|S14182 DNA-directed RNA polymera... 34 0.027
TC16883 similar to UP|Q9STI6 (Q9STI6) Nucleotide sugar epimerase... 34 0.036
AV767357 34 0.036
TC16568 similar to UP|AAR82759 (AAR82759) RE40656p (Fragment), p... 34 0.036
TC19121 similar to UP|ENAH_MOUSE (Q03173) Enabled protein homolo... 32 0.10
TC9801 homologue to UP|Q8LAC6 (Q8LAC6) Glycine rich protein-like... 32 0.10
AV410198 32 0.10
TC8795 similar to UP|GMD1_ARATH (Q9SNY3) GDP-mannose 4,6 dehydra... 31 0.23
AV409241 31 0.23
TC7927 similar to UP|Q93YR3 (Q93YR3) HSP associated protein like... 31 0.23
AV411518 31 0.23
TC14463 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein (At2g... 31 0.23
TC14193 similar to UP|Q8S2S7 (Q8S2S7) Ethylene responsive elemen... 31 0.30
TC8210 homologue to PIR|T10586|T10586 small nuclear ribonucleopr... 30 0.40
>TC15328 weakly similar to UP|Q9LP71 (Q9LP71) T1N15.15, partial (50%)
Length = 1325
Score = 495 bits (1275), Expect = e-141
Identities = 250/313 (79%), Positives = 267/313 (84%), Gaps = 4/313 (1%)
Frame = +3
Query: 1 MATDKSVLAVIRAARPTFRNQNDKIAFVVHSSFLASGYILTATGPQALSDTALSNPSNDE 60
MA+DKSVLAVIRAARPTFRN NDKIAF VHSSFLASGY+LTATGPQALSDTALSNPSNDE
Sbjct: 42 MASDKSVLAVIRAARPTFRNHNDKIAFAVHSSFLASGYVLTATGPQALSDTALSNPSNDE 221
Query: 61 VSVDHWNELNDEYAFVYANSEKEGLK-KVLVKCLVMNGKLLVHALSEGFKEPLHLEINVA 119
VS+D WNEL DEYAFVYAN EK K KVLVKCLV+N KLLVHALS GF PLHLEINV
Sbjct: 222 VSIDQWNELIDEYAFVYANPEKPSNKNKVLVKCLVINDKLLVHALSHGFSNPLHLEINVG 401
Query: 120 DYAGEDGGSNFSQQFKNLDKLVKKIDADILSKLDG-TAHASSSTKSSKTSDSTRPETSEP 178
DYAGEDGGSN+SQ FKN +KLVK+ID++ILSKLDG +A+ASSSTKSS+TSD TR E S+P
Sbjct: 402 DYAGEDGGSNYSQHFKNFEKLVKRIDSEILSKLDGSSANASSSTKSSETSDRTRQEISDP 581
Query: 179 VPGFGEPAGPNDYHPGFIVPPVPAGSGSDLFPGPGAGMYPSRGDHDFGGSMLVGPNDPRW 238
V GFGEPAGP YHP + PPVP GSGSDL PGP AG++PS G GGSM VGPNDPRW
Sbjct: 582 VAGFGEPAGP-PYHPSGVFPPVPIGSGSDLVPGPPAGVFPSSGP-SIGGSMYVGPNDPRW 755
Query: 239 FSGGTGGGIG--GGPGFIGGLPGAPPGARFDPIGPPGVPGFEPNRFARNPRRPGFDAHPD 296
F GG GGG G GGPGF GGLPG PPGARFDP GPPG+PGFEPNRFARNPRRPG D HPD
Sbjct: 756 F-GGVGGGPGFPGGPGFPGGLPGVPPGARFDPYGPPGIPGFEPNRFARNPRRPGNDTHPD 932
Query: 297 LQHFRRDTDSDYI 309
LQHFRRD DSDYI
Sbjct: 933 LQHFRRDADSDYI 971
Score = 31.6 bits (70), Expect = 0.18
Identities = 19/42 (45%), Positives = 21/42 (49%), Gaps = 4/42 (9%)
Frame = -2
Query: 242 GTGGGIGGGP----GFIGGLPGAPPGARFDPIGPPGVPGFEP 279
G+ G+ GGP GG PG PPG GPPG PG P
Sbjct: 880 GSNPGMPGGP*GSNRAPGGTPGNPPGKP----GPPGKPGPPP 767
Score = 26.2 bits (56), Expect = 7.5
Identities = 15/44 (34%), Positives = 17/44 (38%)
Frame = -2
Query: 252 GFIGGLPGAPPGARFDPIGPPGVPGFEPNRFARNPRRPGFDAHP 295
GF+ L G+ PG P G PG P P PG P
Sbjct: 901 GFLANLLGSNPGMPGGP*GSNRAPGGTPGNPPGKPGPPGKPGPP 770
>TC11354 similar to UP|CAE54480 (CAE54480) Alpha glucosidase II , partial
(6%)
Length = 583
Score = 37.0 bits (84), Expect = 0.004
Identities = 24/70 (34%), Positives = 31/70 (44%), Gaps = 5/70 (7%)
Frame = -1
Query: 155 TAHASSSTKSSKTSDSTRPETSEPVPGFGEPAGP-----NDYHPGFIVPPVPAGSGSDLF 209
T ASS++ S+KT STRP + P P+ P + P I PP P S S
Sbjct: 226 TTTASSASPSTKTLPSTRPRPASESPTSSSPSSPPPSSGSSVSPPRISPPPPPPSNSPTA 47
Query: 210 PGPGAGMYPS 219
P + PS
Sbjct: 46 TPPSSATNPS 17
>TC16065 weakly similar to UP|Q9SBM1 (Q9SBM1) Hydroxyproline-rich
glycoprotein DZ-HRGP precursor, partial (20%)
Length = 608
Score = 35.8 bits (81), Expect = 0.009
Identities = 42/136 (30%), Positives = 53/136 (38%), Gaps = 17/136 (12%)
Frame = -1
Query: 157 HASSSTKSSKTSDSTRPETSEPVPGFGEPAGPNDYHPGFIVPPVPA---GSGSDLFPGPG 213
H+ S+ +S+ P+ + PG G P GP G+ PP G G PG G
Sbjct: 428 HSHSTERSAPP-----PQQAAAAPGGGGP-GPQGGR-GYGGPPQGGRGGGYGGGGGPGYG 270
Query: 214 AGMYPSRGDHDFGGSMLVGPNDPRW--------------FSGGTGGGIGGGPGFIGGLPG 259
G RG H GP + + +SGG GGG GGG G GG G
Sbjct: 269 GG---GRGGHGVPQQQYGGPPEYQGRGRGGPSQQGGRGGYSGG-GGGYGGGGGRGGGGMG 102
Query: 260 APPGARFDPIGPPGVP 275
+ G GPP P
Sbjct: 101 SGRGVSPSHGGPPSRP 54
>BP069129
Length = 414
Score = 35.0 bits (79), Expect = 0.016
Identities = 24/59 (40%), Positives = 29/59 (48%), Gaps = 7/59 (11%)
Frame = +3
Query: 225 FGGSMLVGPNDPRWFSGGTGGGIGGGPGFIGGLPGAP-----PGARFDPIGP--PGVPG 276
FG ++ GP DP +G + + G PG GG P AP P F P GP PG PG
Sbjct: 228 FGPAVPAGPRDP---AGPSLPSLPGAPGSPGG-PAAPAWPGCPSLPFGPFGPF*PGTPG 392
>AU240151
Length = 286
Score = 35.0 bits (79), Expect = 0.016
Identities = 24/48 (50%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
Frame = +2
Query: 231 VGPNDPRWFSGGTGGGIGGGPGFIGGLPGAPPGARFDPIGP-PGVPGF 277
+GP P GG GGG G GPG GG G PG GP PG PGF
Sbjct: 110 MGPGGPG--GGGPGGGWGPGPGGPGG--GWGPGPGGPGWGPGPGGPGF 241
Score = 34.3 bits (77), Expect = 0.027
Identities = 28/73 (38%), Positives = 29/73 (39%)
Frame = +2
Query: 185 PAGPNDYHPGFIVPPVPAGSGSDLFPGPGAGMYPSRGDHDFGGSMLVGPNDPRWFSGGTG 244
P GP PG P P G G PGPG P G GP P +F G G
Sbjct: 116 PGGPGGGGPGGGWGPGPGGPGGGWGPGPGG---PGWGP---------GPGGPGFF--GPG 253
Query: 245 GGIGGGPGFIGGL 257
GG G GF GL
Sbjct: 254 GGFFG--GFANGL 286
Score = 30.4 bits (67), Expect = 0.40
Identities = 36/94 (38%), Positives = 38/94 (40%), Gaps = 8/94 (8%)
Frame = +2
Query: 188 PNDYHPGF------IVPPVPAGS--GSDLFPGPGAGMYPSRGDHDFGGSMLVGPNDPRWF 239
PN + P F I+P P S L GPG P G GG GP
Sbjct: 20 PNFHSPWFHHCPEAILPRFPCSVVLAS*LNMGPGG---PGGGGP--GGGWGPGP------ 166
Query: 240 SGGTGGGIGGGPGFIGGLPGAPPGARFDPIGPPG 273
GG GGG G GPG G PG P G F GP G
Sbjct: 167 -GGPGGGWGPGPGGPGWGPG-PGGPGF--FGPGG 256
>AW719960
Length = 449
Score = 35.0 bits (79), Expect = 0.016
Identities = 27/77 (35%), Positives = 30/77 (38%), Gaps = 1/77 (1%)
Frame = +3
Query: 204 SGSDLFPG-PGAGMYPSRGDHDFGGSMLVGPNDPRWFSGGTGGGIGGGPGFIGGLPGAPP 262
+G PG P G YP G + P P + G G G P G PGA P
Sbjct: 108 AGHAAAPGYPAPGQYPPPGGYP---GQYGAPGAPGAYPGAPPAGYPGAP---AGYPGAYP 269
Query: 263 GARFDPIGPPGVPGFEP 279
GA G PG PG P
Sbjct: 270 GA--PAAGYPGAPGGAP 314
Score = 30.8 bits (68), Expect = 0.30
Identities = 19/43 (44%), Positives = 19/43 (44%), Gaps = 1/43 (2%)
Frame = +3
Query: 249 GGPGFIGGLPGAPPGARFDPIGPPGVPGFEPNRFARN-PRRPG 290
G PG G PGAPP P P G PG P A P PG
Sbjct: 183 GAPGAPGAYPGAPPAGY--PGAPAGYPGAYPGAPAAGYPGAPG 305
Score = 27.3 bits (59), Expect = 3.4
Identities = 23/70 (32%), Positives = 25/70 (34%), Gaps = 17/70 (24%)
Frame = -2
Query: 240 SGGTGGGIGGGPGFIGGLPGAP-----------------PGARFDPIGPPGVPGFEPNRF 282
+G G GG PG G PGAP PGA P P +P F F
Sbjct: 247 AGAPG*PAGGAPG*APGAPGAPYWPG*PPGGGYCPGAG*PGAAACPACLPSLP-FPSLPF 71
Query: 283 ARNPRRPGFD 292
P P FD
Sbjct: 70 PSLPFLPSFD 41
>TC19891 homologue to PIR|S14182|S14182 DNA-directed RNA polymerase largest
chain (isoform B2) - soybean (fragment)
{Glycine max;} , partial (34%)
Length = 498
Score = 34.3 bits (77), Expect = 0.027
Identities = 27/107 (25%), Positives = 36/107 (33%)
Frame = +3
Query: 185 PAGPNDYHPGFIVPPVPAGSGSDLFPGPGAGMYPSRGDHDFGGSMLVGPNDPRWFSGGTG 244
P YH G + P L P A P + G M P +S +
Sbjct: 147 PISGTPYHEGMMSPSYMLSPNLRLSPTNDAQFSP------YVGGMAFSPTSSPGYSPSSP 308
Query: 245 GGIGGGPGFIGGLPGAPPGARFDPIGPPGVPGFEPNRFARNPRRPGF 291
G PG+ PG P + P P PG+ P +P PG+
Sbjct: 309 GYSPSSPGYSPTSPGYSPTS---PGYSPTSPGYSPTSPTYSPSSPGY 440
>TC16883 similar to UP|Q9STI6 (Q9STI6) Nucleotide sugar epimerase-like
protein, partial (24%)
Length = 602
Score = 33.9 bits (76), Expect = 0.036
Identities = 24/65 (36%), Positives = 30/65 (45%), Gaps = 3/65 (4%)
Frame = +1
Query: 158 ASSSTKSSKTSDSTRPETSEPVPGF---GEPAGPNDYHPGFIVPPVPAGSGSDLFPGPGA 214
+SSS+ S P ++P PG G+ A PN P VPP P+ S S P A
Sbjct: 319 SSSSSSSPLPHPLPPPPPTKPAPGAAPTGKNASPN---PPAAVPPPPSPSSSPAPPASSA 489
Query: 215 GMYPS 219
M PS
Sbjct: 490 AMSPS 504
>AV767357
Length = 458
Score = 33.9 bits (76), Expect = 0.036
Identities = 22/53 (41%), Positives = 27/53 (50%), Gaps = 2/53 (3%)
Frame = -2
Query: 230 LVGPNDPRWFSGGTGGGIGGGPGFI-GGLPGAPPGARFDPIGPPGVP-GFEPN 280
+V P R+ GGG GG PG + GG+PG PG G P P G +PN
Sbjct: 352 VVNPIMTRFHQSSGGGGPGGIPGGMPGGMPGGMPGGMPGGGGAPMDPSGSDPN 194
>TC16568 similar to UP|AAR82759 (AAR82759) RE40656p (Fragment), partial
(13%)
Length = 421
Score = 33.9 bits (76), Expect = 0.036
Identities = 25/62 (40%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = +3
Query: 199 PVPAGSGSDLFPGPGAGMYPSRGDHDFG-GSMLVGPNDPRWFSGGTGGGIGGGPGFIGGL 257
PVP GAG Y S +++ G GS L G + + GG GGG+GGG G GG+
Sbjct: 252 PVPTRPWEQNTTYGGAGGYGSTMNYNSGYGSGLYGSS----YGGGLGGGLGGGLG--GGM 413
Query: 258 PG 259
G
Sbjct: 414 YG 419
>TC19121 similar to UP|ENAH_MOUSE (Q03173) Enabled protein homolog (NPC
derived proline-rich protein 1) (NDPP-1), partial (3%)
Length = 414
Score = 32.3 bits (72), Expect = 0.10
Identities = 27/69 (39%), Positives = 29/69 (41%), Gaps = 4/69 (5%)
Frame = -2
Query: 198 PPVPAGSG-SDLFPGPGAGMYPSRGDHDFGGSMLVGPNDPRWFSGGTGGG---IGGGPGF 253
P V G G S G G GM P G GS+ SGG GG +G GF
Sbjct: 203 PEVAQGHGNSHGHHGDGMGMAPGGGAGSGAGSL----------SGGQYGGPVGLGSYGGF 54
Query: 254 IGGLPGAPP 262
GGL G PP
Sbjct: 53 GGGLQGQPP 27
>TC9801 homologue to UP|Q8LAC6 (Q8LAC6) Glycine rich protein-like, complete
Length = 981
Score = 32.3 bits (72), Expect = 0.10
Identities = 19/53 (35%), Positives = 21/53 (38%)
Frame = +2
Query: 212 PGAGMYPSRGDHDFGGSMLVGPNDPRWFSGGTGGGIGGGPGFIGGLPGAPPGA 264
PG G RG + G R F G G G G G +GG PG GA
Sbjct: 371 PGVGRGRGRGGREEGAGGRPAKGPGRGFDDGAKGPGGRGRGGLGGKPGGSRGA 529
>AV410198
Length = 337
Score = 32.3 bits (72), Expect = 0.10
Identities = 19/29 (65%), Positives = 20/29 (68%), Gaps = 1/29 (3%)
Frame = -2
Query: 239 FSGGTGGGIG-GGPGFIGGLPGAPPGARF 266
FSGG GGGIG GG G+ GGL G GA F
Sbjct: 288 FSGG-GGGIGSGGGGWGGGLVGEAEGAEF 205
>TC8795 similar to UP|GMD1_ARATH (Q9SNY3) GDP-mannose 4,6 dehydratase
(GDP-D-mannose dehydratase) (GMD) , partial (43%)
Length = 674
Score = 31.2 bits (69), Expect = 0.23
Identities = 17/42 (40%), Positives = 25/42 (59%)
Frame = +1
Query: 159 SSSTKSSKTSDSTRPETSEPVPGFGEPAGPNDYHPGFIVPPV 200
+S T ++ TSD+TRP +P+ G+P PN P FI P+
Sbjct: 556 TSPTPAALTSDTTRP---DPLRCSGQPTRPNRKPPRFIRDPL 672
>AV409241
Length = 282
Score = 31.2 bits (69), Expect = 0.23
Identities = 20/60 (33%), Positives = 26/60 (43%)
Frame = -1
Query: 200 VPAGSGSDLFPGPGAGMYPSRGDHDFGGSMLVGPNDPRWFSGGTGGGIGGGPGFIGGLPG 259
V G + F G G+ + GD GG + G GG GGG+GGG +G G
Sbjct: 234 VDVGIFREGFAESGEGVAAAGGDGVGGGETVAG--------GGGGGGVGGGGVLVGECEG 79
>TC7927 similar to UP|Q93YR3 (Q93YR3) HSP associated protein like, partial
(56%)
Length = 1116
Score = 31.2 bits (69), Expect = 0.23
Identities = 37/118 (31%), Positives = 43/118 (36%), Gaps = 6/118 (5%)
Frame = -2
Query: 152 LDGTAHASSSTKSSKTS-DSTRPETSEPVPGFGEPAG--PNDYHPGFIVPPVPAGSGSDL 208
+ G+ +A+ + S K ST P T +P G P G P PG P P G
Sbjct: 722 ISGSLNAAIRSGSFKILLKSTFPGTPPGMPPPGNPPGMPPPGNPPGMPPPGNPPGMPP-- 549
Query: 209 FPGPGAGMYPSRGDHDFGGSMLVGPNDPRWFSGGTGGGI---GGGPGFIGGLPGAPPG 263
PG GM P N P G GI G PG G PG PPG
Sbjct: 548 -PGKPPGMPPPG-------------NPPGIPPPGNPPGIPPPGNPPGIPGNSPGIPPG 417
>AV411518
Length = 360
Score = 31.2 bits (69), Expect = 0.23
Identities = 20/64 (31%), Positives = 24/64 (37%), Gaps = 6/64 (9%)
Frame = +3
Query: 155 TAHASSSTKSSKTSDSTRPETSE------PVPGFGEPAGPNDYHPGFIVPPVPAGSGSDL 208
T+ +S S T+ S RP S P P P P P +PP A S S
Sbjct: 168 TSSGPASPSPSSTAPSPRPNPSSTSGPTSPTPHTPSPPPPTSSSPSSAIPPTSAPSSSTP 347
Query: 209 FPGP 212
P P
Sbjct: 348 PPAP 359
>TC14463 similar to UP|Q9SLF1 (Q9SLF1) Nodulin-like protein
(At2g16660/T24I21.7), partial (40%)
Length = 1122
Score = 31.2 bits (69), Expect = 0.23
Identities = 20/55 (36%), Positives = 24/55 (43%), Gaps = 1/55 (1%)
Frame = +2
Query: 155 TAHASSSTKSSKTSDSTRPETSEPV-PGFGEPAGPNDYHPGFIVPPVPAGSGSDL 208
T+ +SSST S S +P S P P P GP+ V A SGS L
Sbjct: 242 TSPSSSSTTSPSLKTSVKPSVSSPASPPTSSPLGPSSSSAPSKASSVTASSGSSL 406
>TC14193 similar to UP|Q8S2S7 (Q8S2S7) Ethylene responsive element binding
factor 4-like protein, partial (50%)
Length = 1128
Score = 30.8 bits (68), Expect = 0.30
Identities = 12/21 (57%), Positives = 15/21 (71%)
Frame = -2
Query: 244 GGGIGGGPGFIGGLPGAPPGA 264
GGG+ G GF+GG+ GA P A
Sbjct: 308 GGGVVGSSGFLGGVEGAEPDA 246
>TC8210 homologue to PIR|T10586|T10586 small nuclear
ribonucleoprotein-associated protein homolog
F9F13.90 - Arabidopsis thaliana {Arabidopsis
thaliana;}, partial (16%)
Length = 698
Score = 30.4 bits (67), Expect = 0.40
Identities = 32/102 (31%), Positives = 37/102 (35%)
Frame = +2
Query: 198 PPVPAGSGSDLFPGPGAGMYPSRGDHDFGGSMLVGPNDPRWFSGGTGGGIGGGPGFIGGL 257
PP P GS P PG G P G G +GP P G P G+
Sbjct: 5 PPQPMPPGS-FAPRPG-GPPPQYGVPPQFGQRPMGPPPPGQMMRGPP------PPPRPGM 160
Query: 258 PGAPPGARFDPIGPPGVPGFEPNRFARNPRRPGFDAHPDLQH 299
PG PP P P G P + P RPG P+ Q+
Sbjct: 161 PGPPPPRPGMPPPPGGAPVYA-------PPRPGMPPPPNSQN 265
Score = 28.5 bits (62), Expect = 1.5
Identities = 17/34 (50%), Positives = 18/34 (52%), Gaps = 3/34 (8%)
Frame = -3
Query: 246 GIGGGPGFIGGLPGAPPGARFDP---IGPPGVPG 276
G GG PG G GAPPG P G PG+PG
Sbjct: 252 GGGGMPGRGGA*TGAPPGGGGIPGRGGGGPGIPG 151
Score = 27.7 bits (60), Expect = 2.6
Identities = 29/82 (35%), Positives = 32/82 (38%), Gaps = 7/82 (8%)
Frame = -3
Query: 200 VPAGSGSDLFPGPGAGMYPSRGDHDFGGSMLVGPNDPRWFSGGTGGGIGGGPGFI----G 255
+P G+ PG G P RG GG GP P G G GGGP I G
Sbjct: 240 MPGRGGA*TGAPPGGGGIPGRG----GG----GPGIP-------GRGGGGGPLIICPGGG 106
Query: 256 GLPGAPPGARFDPI---GPPGV 274
G G P P GPPG+
Sbjct: 105 GPIGLCPNCGGTPYCGGGPPGL 40
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.138 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 5,151,664
Number of Sequences: 28460
Number of extensions: 84880
Number of successful extensions: 879
Number of sequences better than 10.0: 110
Number of HSP's better than 10.0 without gapping: 690
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 780
length of query: 309
length of database: 4,897,600
effective HSP length: 90
effective length of query: 219
effective length of database: 2,336,200
effective search space: 511627800
effective search space used: 511627800
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC142224.1