Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC142222.6 - phase: 0 
         (123 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BP074735                                                               28  0.34
TC10904 weakly similar to UP|Q62258 (Q62258) Spi2 proteinase inh...    28  0.59
TC13356 weakly similar to UP|Q9SVG3 (Q9SVG3) Reticuline oxidase-...    26  2.2
BP066896                                                               25  2.9
TC10209 similar to UP|Q9SJQ5 (Q9SJQ5) At2g36500 protein, partial...    25  5.0
TC12325 similar to GB|AAP37767.1|30725490|BT008408 At2g43680 {Ar...    24  8.5

>BP074735 
          Length = 472

 Score = 28.5 bits (62), Expect = 0.34
 Identities = 16/47 (34%), Positives = 21/47 (44%)
 Frame = -3

Query: 63  MKKTKKQRDNELNDGVWQKEILMGGKCEPLDFSGVIHYDINGRQTGE 109
           +K  ++Q +  LN G W  EI   G CE L    + H  I  R   E
Sbjct: 470 IKAWQRQSNKFLNKGDWSMEIEEVGNCEALPLLSLNHVSILCRSVWE 330


>TC10904 weakly similar to UP|Q62258 (Q62258) Spi2 proteinase inhibitor,
           partial (8%)
          Length = 576

 Score = 27.7 bits (60), Expect = 0.59
 Identities = 12/42 (28%), Positives = 24/42 (56%)
 Frame = -3

Query: 74  LNDGVWQKEILMGGKCEPLDFSGVIHYDINGRQTGEVPLRSP 115
           +N+G  + +I + G+C  LD + ++   ++G +    PL SP
Sbjct: 460 INNGARKIKIKINGRCSGLDEAFILKSRLSGEEHSVFPLLSP 335


>TC13356 weakly similar to UP|Q9SVG3 (Q9SVG3) Reticuline oxidase-like
           protein, partial (16%)
          Length = 433

 Score = 25.8 bits (55), Expect = 2.2
 Identities = 16/35 (45%), Positives = 21/35 (59%)
 Frame = +1

Query: 3   NLTSSPESLSPKRQSNSSFFITISAILAMLSRQAR 37
           NLTS P S +PK   +SS     SA  AM +R++R
Sbjct: 295 NLTSKPPSSAPKPLKSSS----KSAAAAMTTRESR 387


>BP066896 
          Length = 440

 Score = 25.4 bits (54), Expect = 2.9
 Identities = 13/32 (40%), Positives = 17/32 (52%)
 Frame = -1

Query: 48  LLSNMSNKALSQFGKMKKTKKQRDNELNDGVW 79
           LLS  SN  LS  GK  +T +   +E   G+W
Sbjct: 272 LLSTGSNLGLSGTGKTSETTRNLGDENGRGLW 177


>TC10209 similar to UP|Q9SJQ5 (Q9SJQ5) At2g36500 protein, partial (26%)
          Length = 659

 Score = 24.6 bits (52), Expect = 5.0
 Identities = 10/24 (41%), Positives = 17/24 (70%)
 Frame = +1

Query: 50  SNMSNKALSQFGKMKKTKKQRDNE 73
           +N ++K    + KMKKTK+QR ++
Sbjct: 124 NNTNSKNYRDYYKMKKTKQQRSSK 195


>TC12325 similar to GB|AAP37767.1|30725490|BT008408 At2g43680 {Arabidopsis
          thaliana;}, partial (13%)
          Length = 845

 Score = 23.9 bits (50), Expect = 8.5
 Identities = 13/49 (26%), Positives = 26/49 (52%)
 Frame = +2

Query: 2  ANLTSSPESLSPKRQSNSSFFITISAILAMLSRQARRLKGKAKTKKLLS 50
          AN    P  LS K  + + FF+ +S+  +   ++ + +    KTKK+++
Sbjct: 2  ANGYGPPCKLSEKVANQAIFFLFLSSSSSWNQKKVKLIDKPHKTKKIIN 148


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.315    0.130    0.363 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,670,678
Number of Sequences: 28460
Number of extensions: 16552
Number of successful extensions: 100
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 99
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 100
length of query: 123
length of database: 4,897,600
effective HSP length: 79
effective length of query: 44
effective length of database: 2,649,260
effective search space: 116567440
effective search space used: 116567440
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 49 (23.5 bits)


Medicago: description of AC142222.6