BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC142142.4 - phase: 0
(508 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
NP459548 PSII 47KDa protein [Lotus japonicus] 1039 0.0
TC14174 UP|PSBC_LOTJA (Q9BBT1) Photosystem II 44 kDa reaction ce... 94 4e-20
TC11913 weakly similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding p... 32 0.19
BI419379 30 1.2
TC16160 28 2.7
AV410259 28 3.5
AV766583 27 7.9
TC7970 similar to UP|ADK2_ARATH (Q9LZG0) Adenosine kinase 2 (AK... 27 7.9
>NP459548 PSII 47KDa protein [Lotus japonicus]
Length = 1527
Score = 1039 bits (2686), Expect = 0.0
Identities = 493/508 (97%), Positives = 503/508 (98%)
Frame = +1
Query: 1 MGLPWYRVHTVVLNDPGRLLSVHIMHTALVAGWAGSMALYELAVFDPSDPVLDPMWRQGM 60
MGLPWYRVHTVVLNDPGRLL+VHIMHTALVAGWAGSMALYELAVFDPSDPVLDPMWRQGM
Sbjct: 1 MGLPWYRVHTVVLNDPGRLLAVHIMHTALVAGWAGSMALYELAVFDPSDPVLDPMWRQGM 180
Query: 61 FVIPFMTRLGITNSWGGWNITGGTITNPGIWSYEGVAAAHIVFSGLCFLAAIWHWVYWDL 120
FVIPFMTRLGITNSWGGWNITGGTITNPGIWSYEGVA AHIVFSGLCFLAAIWHWVYWDL
Sbjct: 181 FVIPFMTRLGITNSWGGWNITGGTITNPGIWSYEGVAGAHIVFSGLCFLAAIWHWVYWDL 360
Query: 121 EIFCDERTGKPSLDLPKIFGIHLFLAGVACFGFGAFHVTGLFGPGIWVSDPYGLTGRVQS 180
EIF DERTGKPSLDLPKIFGIHLFLAG+ACFGFGAFHVTGL+GPGIWVSDPYGLTGRVQ
Sbjct: 361 EIFSDERTGKPSLDLPKIFGIHLFLAGLACFGFGAFHVTGLYGPGIWVSDPYGLTGRVQP 540
Query: 181 VNPAWGVDGFDPFVPGGIASHHIAAGTLGILAGLFHLSVRPPQRLYKGLRMGNIETVLSS 240
VNPAWGV+GFDPFVPGG+ASHHIAAGTLGILAGLFHLSVRPPQRLYKGLRMGNIETVLSS
Sbjct: 541 VNPAWGVEGFDPFVPGGVASHHIAAGTLGILAGLFHLSVRPPQRLYKGLRMGNIETVLSS 720
Query: 241 SIAAVFFAAFVVAGTMWYGSATTPIELFGPTRYQWDQGYFQQEIYRRVGAGLAENQSLSE 300
SIAAVFFAAFVVAGTMWYGSATTPIELFGPTRYQWDQGYFQQEIYRRVGAGLAENQS SE
Sbjct: 721 SIAAVFFAAFVVAGTMWYGSATTPIELFGPTRYQWDQGYFQQEIYRRVGAGLAENQSFSE 900
Query: 301 AWSKIPEKLAFYDYIGNNPAKGGLFRAGSMDNGDGIAVGWLGHPIFRDKEGRELFVRRMP 360
AWSKIPEKLAFYDYIGNNPAKGGLFRAGSMDNGDGIAVGWLGHPIFRDKEGRELFVRRMP
Sbjct: 901 AWSKIPEKLAFYDYIGNNPAKGGLFRAGSMDNGDGIAVGWLGHPIFRDKEGRELFVRRMP 1080
Query: 361 TFFETFPVVLVDGDGIVRADVPFRRAESKYSVEQVGVTVEFFGGELNGVSYSDPATVKKY 420
TFFETFPVVLVDGDGIVRADVPFRRAESKYSVEQVGVTVEF+GGELNGVSYSDPATVKKY
Sbjct: 1081TFFETFPVVLVDGDGIVRADVPFRRAESKYSVEQVGVTVEFYGGELNGVSYSDPATVKKY 1260
Query: 421 ARRAQLGEIFELDRATLKSDGVFRSSPRGWFTFGHASFALLFFFGHIWHGARTLFRDVFA 480
ARRAQLGEIFELDRATLKSDGVFRSSPRGWFTFGHASFALLFFFGHIWHG+RTLFRDVFA
Sbjct: 1261ARRAQLGEIFELDRATLKSDGVFRSSPRGWFTFGHASFALLFFFGHIWHGSRTLFRDVFA 1440
Query: 481 GIDPDLDAQVEFGAFQKLGDPTTKKQAV 508
GIDPDLD+QVEFGAFQKLGDPTT++Q V
Sbjct: 1441GIDPDLDSQVEFGAFQKLGDPTTRRQVV 1524
>TC14174 UP|PSBC_LOTJA (Q9BBT1) Photosystem II 44 kDa reaction center protein
(P6 protein) (CP43), complete
Length = 3406
Score = 94.4 bits (233), Expect = 4e-20
Identities = 121/519 (23%), Positives = 191/519 (36%), Gaps = 29/519 (5%)
Frame = +1
Query: 2 GLPWYRVHTVVLNDPGRLLSVHIMHTALVAGWAGSMALYELAVFDPSDPVLDPMWRQGMF 61
G W+ + ++N G+LL H+ H L+ WAG+M L+E+A F P P M+ QG+
Sbjct: 1606 GFAWWAGNARLINLSGKLLGAHVAHAGLIVFWAGAMNLFEVAHFVPEKP----MYEQGLI 1773
Query: 62 VIPFMTRLGITNSWGGWNI-TGGTITNPGIWSYEGVAAAHIVFSGLCFLAAIWHWVYWDL 120
++P + LG W + GG + + + GV H++ S + I+H +
Sbjct: 1774 LLPHLATLG-------WGVGPGGEVIDTFPYFVSGVL--HLISSAVLGFGGIYHALLGPE 1926
Query: 121 EIFCDER------TGKPSLDLPKIFGIHLFLAGVACF-------GFGAFHVTGLFGPGIW 167
+ +E K + I GIHL L G+ F FG + T G G
Sbjct: 1927 TL--EESFPFFGYVWKDRNKMTTILGIHLILLGIGAFLLVFKASYFGGIYDTWAPGGG-- 2094
Query: 168 VSDPYGLTGRVQSVNPAWGVDGFDPFVPGG----------IASHHIAAGTLGILAGLFHL 217
D +T S + +G PF G I H+ G++ IL G++H+
Sbjct: 2095 --DVRKITNLTLSPSIIFGYLLKSPFGGEGWIVSVDDLEDIIGGHVWLGSICILGGIWHI 2268
Query: 218 SVRPPQRLYKGLRMGNIETVLSSSIAAVFFAAFVVAGTMWYGSATTPIELFGPTRYQWDQ 277
+P + L E LS S+AA+ F+ +W+ + P E +GPT + Q
Sbjct: 2269 LTKPFAWARRALVWSG-EAYLSYSLAALSVFGFIACCFVWFNNTAYPSEFYGPTGPEASQ 2445
Query: 278 GYFQQEIYR--RVGAGLAENQSLSEAWSKIPEKLAFYDYIGNNPAKGGLFRAGSMDNGDG 335
+ R R+GA + Q P L Y+ +P +F +M D
Sbjct: 2446 AQAFTFLVRDQRLGANVGSAQG--------PTGLG--KYLMRSPTGEVIFGGETMRFWD- 2592
Query: 336 IAVGWLGHPIFRDKEGRELFVRRMPTFFETFPVVLVDGDGIVRADVPFRRAESKYSVEQV 395
+ WL P+ R G +L R+ + + R ++Y
Sbjct: 2593 LRAPWL-EPL-RGPNGLDL--SRLKKDIQPWQ----------------ERRSAEYMTHAP 2712
Query: 396 GVTVEFFGG---ELNGVSYSDPATVKKYARRAQLGEIFELDRATLKSDGVFRSSPRGWFT 452
++ GG E+N V+Y P R W
Sbjct: 2713 LGSLNSVGGVATEINAVNYVSP---------------------------------RSWLA 2793
Query: 453 FGHASFALLFFFGHIWHGARTLFRDVFAGIDPDLDAQVE 491
H F GH+WH R R AG + +D E
Sbjct: 2794 TSHFVLGFFLFVGHLWHAGRA--RAAAAGFEKGIDRDFE 2904
>TC11913 weakly similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein
ABP19a precursor, partial (61%)
Length = 530
Score = 32.3 bits (72), Expect = 0.19
Identities = 22/51 (43%), Positives = 25/51 (48%)
Frame = +2
Query: 106 LCFLAAIWHWVYWDLEIFCDERTGKPSLDLPKIFGIHLFLAGVACFGFGAF 156
LC LA W V+W G PSL L K + LFL GVA FGF +
Sbjct: 305 LC-LATTWILVWW---------RGLPSLILLK*RSLRLFLEGVASFGFANY 427
>BI419379
Length = 376
Score = 29.6 bits (65), Expect = 1.2
Identities = 12/33 (36%), Positives = 21/33 (63%)
Frame = -2
Query: 113 WHWVYWDLEIFCDERTGKPSLDLPKIFGIHLFL 145
W WV + EI C +T PS+ + +I+G+++ L
Sbjct: 363 WCWV*SEREIICPIKTPVPSM*IKEIYGLYISL 265
>TC16160
Length = 1164
Score = 28.5 bits (62), Expect = 2.7
Identities = 16/61 (26%), Positives = 29/61 (47%), Gaps = 11/61 (18%)
Frame = -2
Query: 257 WYGSATTPIELFGPTRYQW-------DQGYFQQ----EIYRRVGAGLAENQSLSEAWSKI 305
++G TTP +LF R+QW +G QQ + + ++ G + + + WS +
Sbjct: 962 YFGLQTTPEDLFQILRHQWLRNHGINRRGLLQQKS*YQHFHQMHCGWLDENEIQQ*WSYV 783
Query: 306 P 306
P
Sbjct: 782 P 780
>AV410259
Length = 418
Score = 28.1 bits (61), Expect = 3.5
Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 2/29 (6%)
Frame = +2
Query: 55 MWRQGMFVIPFMTRLGITNSWGG--WNIT 81
MW+ VI FM +LG+ WG W++T
Sbjct: 281 MWQNMWLVILFMVKLGMFGLWGNAIWSLT 367
>AV766583
Length = 378
Score = 26.9 bits (58), Expect = 7.9
Identities = 19/73 (26%), Positives = 31/73 (42%)
Frame = -1
Query: 75 WGGWNITGGTITNPGIWSYEGVAAAHIVFSGLCFLAAIWHWVYWDLEIFCDERTGKPSLD 134
W W + G + WS V +H+V + C++A W ++ L +R K L
Sbjct: 210 WTQWGLVLGEVLKSI*WS---VNISHLVHTKYCYVALRGQWTWFRLVQM--QRINKYYLH 46
Query: 135 LPKIFGIHLFLAG 147
+ + G L AG
Sbjct: 45 ITRSLGSILDCAG 7
>TC7970 similar to UP|ADK2_ARATH (Q9LZG0) Adenosine kinase 2 (AK 2)
(Adenosine 5'-phosphotransferase 2) , partial (97%)
Length = 1482
Score = 26.9 bits (58), Expect = 7.9
Identities = 16/44 (36%), Positives = 20/44 (45%)
Frame = -1
Query: 72 TNSWGGWNITGGTITNPGIWSYEGVAAAHIVFSGLCFLAAIWHW 115
TN G +ITG +T P ++ G A I LC A HW
Sbjct: 936 TNFSLGNSITGNNVTFPSSATHTGSAPCVITVILLCVPDAFGHW 805
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.325 0.143 0.469
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,534,343
Number of Sequences: 28460
Number of extensions: 145846
Number of successful extensions: 810
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 799
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 808
length of query: 508
length of database: 4,897,600
effective HSP length: 94
effective length of query: 414
effective length of database: 2,222,360
effective search space: 920057040
effective search space used: 920057040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 57 (26.6 bits)
Medicago: description of AC142142.4