BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141923.5 - phase: 0 /pseudo
(648 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CN825325 145 3e-35
AV428893 131 3e-31
BP075094 85 6e-20
CB828503 58 5e-09
AV410332 54 1e-07
AV408482 53 1e-07
AV412541 46 2e-05
BP063796 44 8e-05
TC10231 similar to UP|O23110 (O23110) AP2 domain containing prot... 43 2e-04
AV412439 33 0.19
AV767296 32 0.42
TC10150 similar to UP|BAC98494 (BAC98494) AG-motif binding prote... 30 0.93
TC7947 similar to UP|Q9ST69 (Q9ST69) Ribosomal protein S5, parti... 30 0.93
BP067721 29 2.1
TC9242 similar to PIR|B86320|B86320 3-phosphoserine phosphatase ... 29 2.7
AV769823 28 3.5
AV422062 28 4.6
BP069475 28 4.6
BP040430 28 6.0
TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,... 28 6.0
>CN825325
Length = 541
Score = 145 bits (365), Expect = 3e-35
Identities = 90/211 (42%), Positives = 118/211 (55%), Gaps = 4/211 (1%)
Frame = +1
Query: 372 QFRFWPNNNSRMYVLEGVTPCIQSLQLNAGDTVTFSRIDPGEKFLFGFRRSLTSIVTQDA 431
QFRFWPNNNSRMYVLEGVTPCIQS+QL AGDTVTFSR++P + + GFR++ +S + D
Sbjct: 4 QFRFWPNNNSRMYVLEGVTPCIQSMQLQAGDTVTFSRLEPEGRLVMGFRKA-SSAMPSDQ 180
Query: 432 STSSHSNGILIKDTNFSGAPQNLNSLSSFSNLLQSMKGNGEPYLNGHSEHLRLGNGTADW 491
++ NG NG S H G +
Sbjct: 181 DNDTNKNG------------------------------------NGFSAH-----GEVE- 234
Query: 492 LKTANSEEEMNNG---PLQRLVSVSEKKRSRNI-GTKTKRLHIHSEDAMELRLTWEEAQE 547
L NS +++ + L S S KKR +I G+K+KRL I SED +EL++TW+EAQ
Sbjct: 235 LADPNSWSKVDKSGYIAKEALGSKSLKKRKTSILGSKSKRLKIESEDMIELKITWQEAQG 414
Query: 548 FLCPPPSVEPNFVTIEDQVFEEYDEPPVFGK 578
L PPP++ P+ V IE FEEY++ PV GK
Sbjct: 415 LLRPPPNLIPSIVVIEGFEFEEYEDAPVLGK 507
>AV428893
Length = 249
Score = 131 bits (330), Expect = 3e-31
Identities = 62/79 (78%), Positives = 68/79 (85%)
Frame = +2
Query: 362 QDIMGNEWTFQFRFWPNNNSRMYVLEGVTPCIQSLQLNAGDTVTFSRIDPGEKFLFGFRR 421
QD+ GNEWTFQFRFWPNNNSRMYVLEGVTPCIQS+QL AGDTVTFSRIDPG K + GFR+
Sbjct: 5 QDVKGNEWTFQFRFWPNNNSRMYVLEGVTPCIQSMQLCAGDTVTFSRIDPGGKLVMGFRK 184
Query: 422 SLTSIVTQDASTSSHSNGI 440
+ SI QDAS S+ SNGI
Sbjct: 185 ASISIDAQDASISALSNGI 241
>BP075094
Length = 521
Score = 84.7 bits (208), Expect(2) = 6e-20
Identities = 34/68 (50%), Positives = 45/68 (66%)
Frame = -3
Query: 30 FAKLCNKCGLAYENSLFCDKFHRHETGWRKCSNCSKPIHSGCIVSKSSFEYLDFGGITCV 89
FA LC+K G AYE S +CD FH ++GWR+C++C K +HSGC+ KS + LD GG+ C
Sbjct: 357 FADLCDKYGSAYEQSPYCDMFHSQDSGWRECTSCGKRLHSGCVAFKSQLQLLDAGGVCCK 178
Query: 90 TCVKPSQL 97
C S L
Sbjct: 177 NCTSNSGL 154
Score = 30.0 bits (66), Expect(2) = 6e-20
Identities = 10/23 (43%), Positives = 14/23 (60%)
Frame = -2
Query: 6 CMNNYCKEGSTGEWKKGWSLISG 28
CMN C ++ W+K W+L SG
Sbjct: 430 CMNVACASATSARWRKAWALRSG 362
>CB828503
Length = 487
Score = 57.8 bits (138), Expect = 5e-09
Identities = 28/68 (41%), Positives = 45/68 (66%), Gaps = 2/68 (2%)
Frame = -1
Query: 321 LFEKVLSPSDAGRIGRLVLPKACAEAFLPRILQS--EGVPLQFQDIMGNEWTFQFRFWPN 378
+F+KV++PSD G++ RLV+PK AE + P S +G+ L F+D G W F++ +W
Sbjct: 217 MFDKVVTPSDVGKLNRLVIPKQHAEKYFPLDSSSNEKGLLLNFEDRNGKLWRFRYSYW-- 44
Query: 379 NNSRMYVL 386
N+S+ YV+
Sbjct: 43 NSSQSYVM 20
>AV410332
Length = 322
Score = 53.5 bits (127), Expect = 1e-07
Identities = 21/48 (43%), Positives = 31/48 (63%)
Frame = +2
Query: 4 EVCMNNYCKEGSTGEWKKGWSLISGGFAKLCNKCGLAYENSLFCDKFH 51
++C N+ CKE + KK W L +G A+LC++CG A+E FC+ FH
Sbjct: 167 KLCFNSLCKEFKSERPKKAWRLRTGDLAELCDRCGSAFEEGRFCEIFH 310
>AV408482
Length = 274
Score = 53.1 bits (126), Expect = 1e-07
Identities = 33/87 (37%), Positives = 43/87 (48%)
Frame = +2
Query: 434 SSHSNGILIKDTNFSGAPQNLNSLSSFSNLLQSMKGNGEPYLNGHSEHLRLGNGTADWLK 493
S+ NG +T +SG +NL LS +S LLQS KG E +LN S+ G +W
Sbjct: 14 SNMPNGSHSSETTYSGVYENLPILSGYSGLLQSQKGCSETHLNALSKSWNSAGGDMNWHN 193
Query: 494 TANSEEEMNNGPLQRLVSVSEKKRSRN 520
E +G V V EKKR+RN
Sbjct: 194 IDMPEGRKRDGLPVPPVMVPEKKRTRN 274
>AV412541
Length = 304
Score = 45.8 bits (107), Expect = 2e-05
Identities = 27/55 (49%), Positives = 33/55 (59%), Gaps = 5/55 (9%)
Frame = +1
Query: 308 EKLSGDLKSTVV-----PLFEKVLSPSDAGRIGRLVLPKACAEAFLPRILQSEGV 357
E LSG + V PLFEK ++PSD G++ RLV+PK AE P LQS GV
Sbjct: 121 ENLSGSAQGACVRKAREPLFEKAVTPSDVGKLNRLVIPKQHAEKHFP--LQSVGV 279
>BP063796
Length = 497
Score = 43.9 bits (102), Expect = 8e-05
Identities = 18/25 (72%), Positives = 19/25 (76%)
Frame = +1
Query: 18 EWKKGWSLISGGFAKLCNKCGLAYE 42
EWKKGW L SGGFA+LC KCG E
Sbjct: 265 EWKKGWPLRSGGFAQLCCKCGFMEE 339
>TC10231 similar to UP|O23110 (O23110) AP2 domain containing protein RAP2.8
(Fragment), partial (22%)
Length = 707
Score = 42.7 bits (99), Expect = 2e-04
Identities = 22/65 (33%), Positives = 39/65 (59%), Gaps = 1/65 (1%)
Frame = +1
Query: 354 SEGVPLQFQDIMGNEWTFQFRFWPNNNSRMYVL-EGVTPCIQSLQLNAGDTVTFSRIDPG 412
++G+ L F+D+ G W F++ +W N+S+ YVL +G + ++ L AGD V F R
Sbjct: 40 AKGMLLNFEDVGGKVWRFRYSYW--NSSQSYVLTKGWSRFVKEKNLRAGDAVRFHRSTGP 213
Query: 413 EKFLF 417
++ L+
Sbjct: 214 DRQLY 228
>AV412439
Length = 419
Score = 32.7 bits (73), Expect = 0.19
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = +2
Query: 306 ELEKLSGDLKSTVVPLFEKVLSPSDAGRIGRLVL 339
E E D+K P+FEK L+PSD G++ RLV+
Sbjct: 326 ETENDDDDIKE---PMFEKPLTPSDVGKLNRLVI 418
>AV767296
Length = 490
Score = 31.6 bits (70), Expect = 0.42
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = -3
Query: 197 TLENNRPSWETKSIDETLSLKMALGTSSRNSVLPLATEIGEGKLE 241
TLE PS ++ E + K+ G +S +++L ATE+G + E
Sbjct: 215 TLEETAPSRAPAAVSEVVDHKLVRGDTSSDALLAAATEVGLNRAE 81
>TC10150 similar to UP|BAC98494 (BAC98494) AG-motif binding protein-4,
partial (22%)
Length = 552
Score = 30.4 bits (67), Expect = 0.93
Identities = 12/42 (28%), Positives = 22/42 (51%)
Frame = +3
Query: 9 NYCKEGSTGEWKKGWSLISGGFAKLCNKCGLAYENSLFCDKF 50
++C+ T +W+ G G LCN CG+ Y++ C ++
Sbjct: 72 SHCQVQKTPQWRTG----PLGAKTLCNACGVRYKSGRLCSEY 185
>TC7947 similar to UP|Q9ST69 (Q9ST69) Ribosomal protein S5, partial (81%)
Length = 1294
Score = 30.4 bits (67), Expect = 0.93
Identities = 19/62 (30%), Positives = 31/62 (49%), Gaps = 2/62 (3%)
Frame = +1
Query: 544 EAQEFLCPPPSVEPNFVTIEDQVFEEYDEPPVFGKIKTNASPSGPISNVWD--ADRSTLF 601
E E PPPS++ + ED++ Y+E ++G + S G +V D A + T F
Sbjct: 340 EIPEGFVPPPSIDDGPIETEDEIAAAYEE--IYGPAYSGVSVLGNDIHVMDSKAKKDTGF 513
Query: 602 GA 603
G+
Sbjct: 514 GS 519
>BP067721
Length = 410
Score = 29.3 bits (64), Expect = 2.1
Identities = 18/53 (33%), Positives = 22/53 (40%), Gaps = 10/53 (18%)
Frame = +2
Query: 359 LQFQDIMGNEWTFQFR----------FWPNNNSRMYVLEGVTPCIQSLQLNAG 401
L F I W F +R F N S + V+EGV C QS+ N G
Sbjct: 104 LNFYTIFSRAWVFSWRVRILKPNPNQFLNNTCSLVRVVEGVCDCFQSVTRNKG 262
>TC9242 similar to PIR|B86320|B86320 3-phosphoserine phosphatase [imported]
- Arabidopsis thaliana {Arabidopsis
thaliana;}, partial (35%)
Length = 516
Score = 28.9 bits (63), Expect = 2.7
Identities = 13/30 (43%), Positives = 17/30 (56%), Gaps = 2/30 (6%)
Frame = -2
Query: 55 TGWRKCS--NCSKPIHSGCIVSKSSFEYLD 82
T W C +C+ P H C VS S F++LD
Sbjct: 209 TTWSTCLKISCTVPYHR*CFVSTSLFDFLD 120
>AV769823
Length = 306
Score = 28.5 bits (62), Expect = 3.5
Identities = 19/62 (30%), Positives = 26/62 (41%), Gaps = 20/62 (32%)
Frame = +1
Query: 41 YENSLFCDKFHRHETGWRK-----------------CSNCSKPI---HSGCIVSKSSFEY 80
Y+N+LF +FH H +R C N SKPI H ++ S F Y
Sbjct: 97 YKNNLFTSQFHPHPETYRNRVRVRRENGRKWDRR*ICVNISKPIL*AHYQPLLLSSPFSY 276
Query: 81 LD 82
L+
Sbjct: 277 LE 282
>AV422062
Length = 468
Score = 28.1 bits (61), Expect = 4.6
Identities = 14/33 (42%), Positives = 19/33 (57%)
Frame = +3
Query: 616 LFLLCTMVFQVFRFCIRAARSKGTRKSSKNQKR 648
+F C QVF F +G+RKSS+NQ+R
Sbjct: 99 MF*KCLGYKQVFLFYTTGTEIRGSRKSSENQQR 197
>BP069475
Length = 442
Score = 28.1 bits (61), Expect = 4.6
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = +3
Query: 276 MISHPPRRPPADVKGKNQLLSRYWPRIT 303
+I P +R P V+ + LLS YW R T
Sbjct: 66 LIPEPSKRNPLSVEENDLLLSLYWSRFT 149
>BP040430
Length = 516
Score = 27.7 bits (60), Expect = 6.0
Identities = 13/41 (31%), Positives = 23/41 (55%)
Frame = +3
Query: 150 SRWPQAQRDAMVSCIGPKTEEVKCQFNKEDTRFLNVMKHSS 190
SR + +M+SC+ P+T V F+ RF + ++H+S
Sbjct: 369 SRSSFSSSSSMISCVSPRTVMVLKGFHWGSLRFFSHLEHTS 491
>TC8093 homologue to UP|EFTU_TOBAC (P41342) Elongation factor Tu,
chloroplast precursor (EF-Tu), partial (58%)
Length = 1146
Score = 27.7 bits (60), Expect = 6.0
Identities = 25/112 (22%), Positives = 49/112 (43%)
Frame = +1
Query: 185 VMKHSSHLTAFTTLENNRPSWETKSIDETLSLKMALGTSSRNSVLPLATEIGEGKLEGKA 244
+ K S +L F+ L ++ S + SI T+++ A TSS + L +
Sbjct: 4 ISKPSFYLLLFSFLIHSH-SKTSPSIQPTMAISAASATSSSSKFLH--------PHPSPS 156
Query: 245 SSHFQQGQTSQSILAQLSKTGIAMNLETNKGMISHPPRRPPADVKGKNQLLS 296
SSH + QL T ++ + + +++ PP++P A ++ L+
Sbjct: 157 SSHSHSSSPCLFLRPQLPTTHLSSSFNPSTILLNAPPQQPSAPFHRRSSSLT 312
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.133 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,336,478
Number of Sequences: 28460
Number of extensions: 192824
Number of successful extensions: 969
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 959
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 968
length of query: 648
length of database: 4,897,600
effective HSP length: 96
effective length of query: 552
effective length of database: 2,165,440
effective search space: 1195322880
effective search space used: 1195322880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC141923.5