
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141923.1 + phase: 0 /pseudo
(83 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9704 similar to UP|AAQ10732 (AAQ10732) Delta-8-sphingolipid de... 27 0.86
BP041937 26 1.1
TC10414 similar to GB|AAP13363.1|30023660|BT006255 At5g55710 {Ar... 26 1.5
BP042448 25 2.5
TC18098 homologue to UP|AAR24658 (AAR24658) At1g76260, partial (7%) 25 2.5
TC8540 similar to GB|AAM16209.1|20334906|AY094053 AT5g02020/T7H2... 25 3.3
BP065657 22 4.1
BP075619 24 4.3
TC16376 similar to UP|Q9LVD9 (Q9LVD9) Gb|AAC28507.1, partial (17%) 24 4.3
TC15411 similar to UP|Q8H2B9 (Q8H2B9) 60s acidic ribosomal prote... 24 5.6
TC15740 weakly similar to GB|AAM19935.1|20453393|AY097419 AT5g58... 23 7.3
CB827211 23 7.3
>TC9704 similar to UP|AAQ10732 (AAQ10732) Delta-8-sphingolipid desaturase,
partial (25%)
Length = 848
Score = 26.6 bits (57), Expect = 0.86
Identities = 8/28 (28%), Positives = 17/28 (60%)
Frame = +2
Query: 18 IWFIAAAFLLISCVGTSCIKESEKHIYV 45
+WF + L +GTSC++ +H+++
Sbjct: 548 LWFNIVLYCLCFFLGTSCLRLGSEHMFI 631
>BP041937
Length = 430
Score = 26.2 bits (56), Expect = 1.1
Identities = 11/27 (40%), Positives = 15/27 (54%)
Frame = +1
Query: 8 GNFKEQQWHTIWFIAAAFLLISCVGTS 34
G F + WH F +A FLL + G+S
Sbjct: 40 GPFADIMWHICAFTSAGFLLFNAQGSS 120
>TC10414 similar to GB|AAP13363.1|30023660|BT006255 At5g55710 {Arabidopsis
thaliana;}, partial (34%)
Length = 504
Score = 25.8 bits (55), Expect = 1.5
Identities = 13/35 (37%), Positives = 19/35 (54%), Gaps = 1/35 (2%)
Frame = -2
Query: 14 QWHTIWFIAAAFLLISCVGT-SCIKESEKHIYVRR 47
+W++ WF LISCV I+E EK + +R
Sbjct: 113 EWNSPWFSVLRKGLISCVSNFGRIREDEKRVREKR 9
>BP042448
Length = 483
Score = 25.0 bits (53), Expect = 2.5
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = -1
Query: 9 NFKEQQWHTIWFIAAAFLLISCVGTSCIKESEKH 42
+ ++ ++HT F+A L+ VGT C+ E H
Sbjct: 372 HLQKMKFHTYSFVATICHLLVYVGTFCLSGLEIH 271
>TC18098 homologue to UP|AAR24658 (AAR24658) At1g76260, partial (7%)
Length = 471
Score = 25.0 bits (53), Expect = 2.5
Identities = 11/34 (32%), Positives = 19/34 (55%)
Frame = +2
Query: 9 NFKEQQWHTIWFIAAAFLLISCVGTSCIKESEKH 42
+ ++ ++HT F+A L+ VGT C+ E H
Sbjct: 80 HLQKMKFHTYSFVATICHLLVYVGTFCLSGLEIH 181
>TC8540 similar to GB|AAM16209.1|20334906|AY094053 AT5g02020/T7H20_70
{Arabidopsis thaliana;}, partial (17%)
Length = 607
Score = 24.6 bits (52), Expect = 3.3
Identities = 11/26 (42%), Positives = 16/26 (61%)
Frame = +3
Query: 7 GGNFKEQQWHTIWFIAAAFLLISCVG 32
GG +KE + IWF+ L+SC+G
Sbjct: 240 GGKWKEGK---IWFLLLLLSLLSCLG 308
>BP065657
Length = 250
Score = 21.6 bits (44), Expect(2) = 4.1
Identities = 8/20 (40%), Positives = 13/20 (65%)
Frame = +3
Query: 28 ISCVGTSCIKESEKHIYVRR 47
+SCV +K+ H++VRR
Sbjct: 189 LSCVKKGYMKDDYIHLFVRR 248
Score = 21.2 bits (43), Expect(2) = 4.1
Identities = 7/14 (50%), Positives = 9/14 (64%)
Frame = +1
Query: 11 KEQQWHTIWFIAAA 24
+ +QW T W I AA
Sbjct: 94 RTEQWRTKWLILAA 135
>BP075619
Length = 409
Score = 24.3 bits (51), Expect = 4.3
Identities = 8/21 (38%), Positives = 14/21 (66%)
Frame = -1
Query: 27 LISCVGTSCIKESEKHIYVRR 47
L++C SC+ +S +IY+ R
Sbjct: 70 LVACTFDSCLGQSHSYIYILR 8
>TC16376 similar to UP|Q9LVD9 (Q9LVD9) Gb|AAC28507.1, partial (17%)
Length = 550
Score = 24.3 bits (51), Expect = 4.3
Identities = 9/18 (50%), Positives = 10/18 (55%)
Frame = +1
Query: 15 WHTIWFIAAAFLLISCVG 32
WHTIW + L I C G
Sbjct: 85 WHTIWCSSGFLLQIWCYG 138
>TC15411 similar to UP|Q8H2B9 (Q8H2B9) 60s acidic ribosomal protein,
complete
Length = 595
Score = 23.9 bits (50), Expect = 5.6
Identities = 8/21 (38%), Positives = 12/21 (57%)
Frame = -3
Query: 8 GNFKEQQWHTIWFIAAAFLLI 28
G++ +W IW A FLL+
Sbjct: 71 GDYLHFRWIAIWIFAVGFLLV 9
>TC15740 weakly similar to GB|AAM19935.1|20453393|AY097419 AT5g58640/mzn1_90
{Arabidopsis thaliana;}, partial (65%)
Length = 575
Score = 23.5 bits (49), Expect = 7.3
Identities = 13/36 (36%), Positives = 19/36 (52%)
Frame = +2
Query: 13 QQWHTIWFIAAAFLLISCVGTSCIKESEKHIYVRRN 48
Q W + F + +L S V TSC+ S ++RRN
Sbjct: 74 QPWIALSFCCSVYLSSSSVLTSCL-SSHLRRHLRRN 178
>CB827211
Length = 545
Score = 23.5 bits (49), Expect = 7.3
Identities = 9/26 (34%), Positives = 14/26 (53%)
Frame = -2
Query: 5 LEGGNFKEQQWHTIWFIAAAFLLISC 30
LE +F+ WH F A + ++SC
Sbjct: 349 LEWRSFRNSGWHNRLFCALRWKVVSC 272
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.368 0.161 0.647
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,595,068
Number of Sequences: 28460
Number of extensions: 20091
Number of successful extensions: 336
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 336
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 336
length of query: 83
length of database: 4,897,600
effective HSP length: 59
effective length of query: 24
effective length of database: 3,218,460
effective search space: 77243040
effective search space used: 77243040
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.7 bits)
S2: 48 (23.1 bits)
Medicago: description of AC141923.1