BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141862.23 + phase: 0
(295 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium t... 145 8e-36
BP068691 81 2e-16
CB827992 66 6e-12
TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4... 66 8e-12
TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding pro... 65 1e-11
BP078908 64 4e-11
TC10885 63 7e-11
BP076661 60 6e-10
CB827320 58 2e-09
BP034811 53 5e-08
TC16664 similar to UP|BAD09830 (BAD09830) DNA-binding protein-li... 50 6e-07
BP039904 49 1e-06
BP036382 48 2e-06
TC18373 46 9e-06
CB828026 46 9e-06
TC12218 45 1e-05
BP036451 45 2e-05
TC9193 similar to GB|AAN28890.1|23506061|AY143951 At4g02590/T10P... 44 4e-05
TC15321 similar to UP|BAD09664 (BAD09664) BHLH protein family-li... 40 4e-04
TC10393 40 4e-04
>TC8661 weakly similar to UP|O81348 (O81348) Symbiotic ammonium
transporter, partial (34%)
Length = 1007
Score = 145 bits (366), Expect = 8e-36
Identities = 81/157 (51%), Positives = 115/157 (72%), Gaps = 5/157 (3%)
Frame = +1
Query: 139 QDHVMAERRRREKLSQRFISLSSLLPGLKKMDKATILEDAIKHLKQLNERVKTLEEHVAD 198
QDH++AERRRREKLSQ I+L++L+PGLKKMDKA++L DAIK++K L ER++ LEE +
Sbjct: 25 QDHIIAERRRREKLSQSLIALAALIPGLKKMDKASVLGDAIKYVKVLKERLRLLEEQNKN 204
Query: 199 KKVESAVFM-KRSILFEEDDRSSCDE----NSDQSLSKIEARVSGKDMLIRIHGDKHCGR 253
+ +ES V + K I +++ SSCD+ S+++L +EARVS KD+L+R+H K G
Sbjct: 205 RAMESVVVVNKPQISNDDNSSSSCDDGTIIGSEEALPHVEARVSEKDVLLRLHCKKQKG- 381
Query: 254 TATAILNELEKHHLSVQSSSILPFGNNYLDITIVAQV 290
IL E++ HL V +SS+LPFG++ LDITIVAQ+
Sbjct: 382 LLLKILFEIQNLHLFVVNSSVLPFGDSILDITIVAQM 492
>BP068691
Length = 500
Score = 80.9 bits (198), Expect = 2e-16
Identities = 50/117 (42%), Positives = 74/117 (62%), Gaps = 5/117 (4%)
Frame = -1
Query: 182 LKQLNERVKTLEEHVADKKVESAVFMKRSILFEEDDRSS-CDEN----SDQSLSKIEARV 236
+K L ER++ LEE + +ES + + + +D+ SS CD+ S+++L +EARV
Sbjct: 500 VKVLKERLRLLEEQNQHRAMESVLVVNNHQISNDDNSSSSCDDGTIIGSEEALPHLEARV 321
Query: 237 SGKDMLIRIHGDKHCGRTATAILNELEKHHLSVQSSSILPFGNNYLDITIVAQVKFI 293
S KD+L+R+H K G IL E++ HL V +SS+LPFG++ LDITIVAQV I
Sbjct: 320 SEKDVLLRLHCQKQKG-LLLKILFEIQNLHLFVVNSSVLPFGDSILDITIVAQVNNI 153
>CB827992
Length = 545
Score = 66.2 bits (160), Expect = 6e-12
Identities = 53/137 (38%), Positives = 71/137 (51%), Gaps = 23/137 (16%)
Frame = +3
Query: 1 MEDTTLFSDHEYQMDSYAFQFDDMAYFKSFSESPQESTYSSHTNINNKRIHSESTQNS-- 58
ME+ T F H++ MDS +F D+ F+SF+ SP ES YSS NKR +SES S
Sbjct: 159 MEEPTYF--HQFPMDSSYAEFQDLE-FESFTASPDES-YSS----GNKRFNSESPDYSFA 314
Query: 59 -SFPTQSPDQSVASATPPTKLL-------KASPKIISFDYSNNDS-------------KV 97
+ PT+ P + T L+ +SPKIISF++SN S V
Sbjct: 315 PARPTKQPKTETTWSAYGTDLIASKASSSSSSPKIISFEHSNVSSVTSSQPFHNMGAANV 494
Query: 98 KKPKTEIGYGENLNFGS 114
KPK E G+GENL+F +
Sbjct: 495 VKPKMESGFGENLDFAA 545
>TC18919 similar to GB|AAN73298.1|25141207|BT002301 At4g16430/dl4240w
{Arabidopsis thaliana;}, partial (29%)
Length = 604
Score = 65.9 bits (159), Expect = 8e-12
Identities = 33/71 (46%), Positives = 49/71 (68%)
Frame = +3
Query: 123 KRENKVSAVNRNPIQAQDHVMAERRRREKLSQRFISLSSLLPGLKKMDKATILEDAIKHL 182
KR K + P+ +HV AER+RREKL+QRF +L +++P + KMDKA++L DAI H+
Sbjct: 369 KRGRKPANGREEPL---NHVEAERQRREKLNQRFYALRAVVPNISKMDKASLLGDAITHI 539
Query: 183 KQLNERVKTLE 193
L +++K LE
Sbjct: 540 TDLQKKIKLLE 572
>TC9411 similar to UP|Q41102 (Q41102) Phaseolin G-box binding protein PG2
(Fragment), partial (30%)
Length = 705
Score = 65.1 bits (157), Expect = 1e-11
Identities = 52/165 (31%), Positives = 81/165 (48%), Gaps = 18/165 (10%)
Frame = +2
Query: 123 KRENKVSAVNRNPIQAQDHVMAERRRREKLSQRFISLSSLLPGLKKMDKATILEDAIKHL 182
KR K + P+ +HV AER+RREKL+QRF +L +++P + KMDKA++L DAI ++
Sbjct: 146 KRGRKPANGREEPL---NHVEAERQRREKLNQRFYALRAVVPNVSKMDKASLLGDAISYI 316
Query: 183 KQLNERVKTLEEHVADKKVESAVFMKRSILFEEDDR-------------SSCDENSDQSL 229
+L ++++ E + + K E SS +S+Q L
Sbjct: 317 TELKTKLQSSESDKTGLQKQFDAMKKELEKTSEQSSSPTPPPPNKNKSFSSSSSSSNQIL 496
Query: 230 -SKIEARVSGKDMLIRIHGDK--HCGRTATAILNE--LEKHHLSV 269
I+ ++ G D +IR+ K H A L E LE +H SV
Sbjct: 497 VEDIDVKIIGWDAMIRVQCSKKNHPAAILMAALMELDLEVNHASV 631
>BP078908
Length = 443
Score = 63.5 bits (153), Expect = 4e-11
Identities = 34/53 (64%), Positives = 43/53 (80%), Gaps = 1/53 (1%)
Frame = -1
Query: 239 KDMLIRIHGDKHCGRTATAILNELEKH-HLSVQSSSILPFGNNYLDITIVAQV 290
+D+LI+IH DK G AT IL ELEKH +L+VQSSSILPFGNN D+TI+A++
Sbjct: 443 EDLLIKIHCDKQSGCAAT-ILRELEKHDYLTVQSSSILPFGNNITDVTIIAKM 288
>TC10885
Length = 573
Score = 62.8 bits (151), Expect = 7e-11
Identities = 39/99 (39%), Positives = 56/99 (56%), Gaps = 9/99 (9%)
Frame = +2
Query: 201 VESAVFMKRSILFEEDDR---------SSCDENSDQSLSKIEARVSGKDMLIRIHGDKHC 251
VE + +K+ L +D SS ++ L +I AR GK++LI IH +K
Sbjct: 26 VEKVIVIKKPDLRGNEDTTNSGDIITTSSDHDDCSTILPEIAARALGKEVLIEIHCEKEN 205
Query: 252 GRTATAILNELEKHHLSVQSSSILPFGNNYLDITIVAQV 290
G T IL+ LE HLSV SS+LPFGN+ L +T++AQ+
Sbjct: 206 G-TELKILDHLENLHLSVNGSSVLPFGNSALSVTVIAQM 319
>BP076661
Length = 532
Score = 59.7 bits (143), Expect = 6e-10
Identities = 29/36 (80%), Positives = 33/36 (91%)
Frame = -3
Query: 256 TAILNELEKHHLSVQSSSILPFGNNYLDITIVAQVK 291
+AIL ELEKHHL+VQS+SILPFGNN LDITIV Q+K
Sbjct: 530 SAILVELEKHHLTVQSTSILPFGNNTLDITIVTQMK 423
>CB827320
Length = 522
Score = 58.2 bits (139), Expect = 2e-09
Identities = 23/61 (37%), Positives = 47/61 (76%)
Frame = +1
Query: 138 AQDHVMAERRRREKLSQRFISLSSLLPGLKKMDKATILEDAIKHLKQLNERVKTLEEHVA 197
A ++++ER RR+KL++R +L S++P + KMDKA+I++DAI++++ L+E+ K ++ +
Sbjct: 223 ASKNIVSERNRRKKLNERLFALRSVVPNISKMDKASIIKDAIEYIEHLHEQEKIIQAEIM 402
Query: 198 D 198
+
Sbjct: 403 E 405
>BP034811
Length = 578
Score = 53.1 bits (126), Expect = 5e-08
Identities = 49/145 (33%), Positives = 75/145 (50%), Gaps = 22/145 (15%)
Frame = +3
Query: 31 SESPQESTYSSHTNINNKRIHSESTQNSSFPTQSPDQSVASATP----------PTKLLK 80
SE+ +++ S T +N HS ST S+F T+ ++++++ P+K LK
Sbjct: 156 SEAQEQNLQRSFTIDSN---HSHSTTTSTFKTEHCSSTMSNSSSGEDDNTTSERPSKTLK 326
Query: 81 ---ASPK-IISFDYSN-----NDSKVKKPKTEIGYGENLNFG---SVISQGDYYKRENKV 128
ASP I+SF N N KPK++ N+N S+ SQ KR+ +
Sbjct: 327 MSNASPSYILSFSNENPAHILNFDSTLKPKSK-ALTVNVNHHGNRSLESQKKETKRKVQE 503
Query: 129 SAVNRNPIQAQDHVMAERRRREKLS 153
R+P QAQDH++AER+RREK+S
Sbjct: 504 GEKARSPHQAQDHIIAERKRREKIS 578
>TC16664 similar to UP|BAD09830 (BAD09830) DNA-binding protein-like, partial
(22%)
Length = 1067
Score = 49.7 bits (117), Expect = 6e-07
Identities = 25/70 (35%), Positives = 40/70 (56%)
Frame = +1
Query: 125 ENKVSAVNRNPIQAQDHVMAERRRREKLSQRFISLSSLLPGLKKMDKATILEDAIKHLKQ 184
E K + R+ ++H AERRRR +++ +L +++PG KMDKA++L + I HLK+
Sbjct: 244 ERKGVSPERSIEALRNHSEAERRRRARINAHLDTLRTVIPGANKMDKASLLAEVITHLKE 423
Query: 185 LNERVKTLEE 194
L E
Sbjct: 424 LKTNAAQASE 453
>BP039904
Length = 544
Score = 48.9 bits (115), Expect = 1e-06
Identities = 31/84 (36%), Positives = 48/84 (56%), Gaps = 7/84 (8%)
Frame = -2
Query: 214 EEDDRSSCDENSDQSLSK-------IEARVSGKDMLIRIHGDKHCGRTATAILNELEKHH 266
E+ +S + N+ + L + ++ARV ++LI IH ++ G IL+ LE
Sbjct: 531 EDTPTTSSETNNSEDLCRPRELFPDVKARVLENEVLIVIHCERENG-IELKILDLLENLR 355
Query: 267 LSVQSSSILPFGNNYLDITIVAQV 290
L V SS+LPFGN+ L ITI+AQ+
Sbjct: 354 LCVTGSSVLPFGNSTLSITIIAQI 283
>BP036382
Length = 511
Score = 47.8 bits (112), Expect = 2e-06
Identities = 25/57 (43%), Positives = 39/57 (67%), Gaps = 1/57 (1%)
Frame = +3
Query: 137 QAQD-HVMAERRRREKLSQRFISLSSLLPGLKKMDKATILEDAIKHLKQLNERVKTL 192
QA D H +AER RRE++++R +L L+P K DKA++L++ I ++K L +VK L
Sbjct: 120 QATDPHSIAERLRRERIAERMKALQELVPNANKTDKASMLDEIIDYVKFLQLQVKVL 290
>TC18373
Length = 437
Score = 45.8 bits (107), Expect = 9e-06
Identities = 27/52 (51%), Positives = 36/52 (68%), Gaps = 2/52 (3%)
Frame = +1
Query: 145 ERRRREKLSQRFISLSSLL-PGLK-KMDKATILEDAIKHLKQLNERVKTLEE 194
E++RREKL++RF LSS+L PG K DK IL+DAI+ L QL + L+E
Sbjct: 262 EKQRREKLNERFCELSSVLEPGRPVKTDKPAILDDAIRVLSQLKTEAQELKE 417
>CB828026
Length = 542
Score = 45.8 bits (107), Expect = 9e-06
Identities = 34/110 (30%), Positives = 52/110 (46%), Gaps = 3/110 (2%)
Frame = +2
Query: 91 SNNDSKVKKPKTEIGY--GENLNFGSVISQGDYYKRENKVSAVNRNPIQAQDHVMAERRR 148
S + K K T G+N GS S +E + R H +AER R
Sbjct: 2 SESQQKADKQTTSAAKASGKNAKHGSQASDP---AKEEYIHVRARRGQATNSHSLAERVR 172
Query: 149 REKLSQRFISLSSLLPGLKKM-DKATILEDAIKHLKQLNERVKTLEEHVA 197
REK+S+R L L+PG K+ KA +L++ I +++ L +V+ L +A
Sbjct: 173 REKISERMKFLQDLVPGCSKVTGKAVMLDEIINYVQSLQRQVEFLSMKLA 322
>TC12218
Length = 737
Score = 45.4 bits (106), Expect = 1e-05
Identities = 31/95 (32%), Positives = 55/95 (57%), Gaps = 4/95 (4%)
Frame = +2
Query: 145 ERRRREKLSQRFISLSSLL-PGLK-KMDKATILEDAIKHLKQLNERVKTLEEHVAD--KK 200
E+ RR+KL++RF+ LSS+L PG + K DKA I+ DA++ + QL + L+E D +K
Sbjct: 290 EKLRRDKLNERFLELSSILEPGRQPKTDKAAIISDAVRVVTQLRNEAEKLKEMNNDLQEK 469
Query: 201 VESAVFMKRSILFEEDDRSSCDENSDQSLSKIEAR 235
++ K I D+++ + ++ K++ R
Sbjct: 470 IKELKAEKNEI---RDEKNKLKLDKEKLEKKVKLR 565
>BP036451
Length = 511
Score = 44.7 bits (104), Expect = 2e-05
Identities = 36/125 (28%), Positives = 69/125 (54%), Gaps = 4/125 (3%)
Frame = +2
Query: 74 PPT---KLLKASPKIISFDYSNNDSKVKKPKTEIGYGENLNFGSVISQGDYYKRE-NKVS 129
PPT +L ++ P+ SF S+ + + G G+ ++ G SQ D E N ++
Sbjct: 128 PPTAFRELFQSLPRGYSFPMSSQNGSIFG-----GEGDEID-GDGGSQLDMGVLEFNTIN 289
Query: 130 AVNRNPIQAQDHVMAERRRREKLSQRFISLSSLLPGLKKMDKATILEDAIKHLKQLNERV 189
V + + ++ E+ RRE+L+ ++ L SL+P K+D+A+++ DAI ++++L +
Sbjct: 290 RVTASVGKGREGKGTEKERREQLNGKYKILRSLIPSPTKIDRASVVGDAIDYIREL---I 460
Query: 190 KTLEE 194
+TL E
Sbjct: 461 RTLNE 475
>TC9193 similar to GB|AAN28890.1|23506061|AY143951 At4g02590/T10P11_13
{Arabidopsis thaliana;}, partial (59%)
Length = 1474
Score = 43.5 bits (101), Expect = 4e-05
Identities = 22/57 (38%), Positives = 38/57 (66%), Gaps = 1/57 (1%)
Frame = +1
Query: 137 QAQD-HVMAERRRREKLSQRFISLSSLLPGLKKMDKATILEDAIKHLKQLNERVKTL 192
QA D H +AER RRE++++R + L+P + K D+A +L++ + ++K L +VK L
Sbjct: 451 QATDPHSIAERLRRERIAERIRA*QELVPSVNKTDRAAMLDEIVDYVKFLRLQVKVL 621
>TC15321 similar to UP|BAD09664 (BAD09664) BHLH protein family-like, partial
(23%)
Length = 1593
Score = 40.4 bits (93), Expect = 4e-04
Identities = 39/162 (24%), Positives = 72/162 (44%), Gaps = 32/162 (19%)
Frame = +2
Query: 64 SPDQSVASATPP--TKLLKASPKIISFDYSNNDSKVKKPKTEIGYGENL-NFGSVI---- 116
SP+ A++TPP T L S + ++S+ S + + E + N+G +
Sbjct: 68 SPETLTATSTPPVNTPLSINSSTPFALNFSSGVSPFRSKFSSFEDSELVSNWGGTLLRAT 247
Query: 117 -------SQGDYYKRENKVSAVNRNPIQAQD-----------------HVMAERRRREKL 152
+ D Y+ E+ S+ P A + H + E+RRR K+
Sbjct: 248 MKAGKSNQEEDEYEEEDFGSSQKHGPSSAPNTNKDAKATDKASAIRSKHSVTEQRRRSKI 427
Query: 153 SQRFISLSSLLP-GLKKMDKATILEDAIKHLKQLNERVKTLE 193
++RF L ++P +K D A+ L + I++++ L E+V+ E
Sbjct: 428 NERFQILRDIIPHSEQKRDTASFLLEVIEYVQYLQEKVQKYE 553
>TC10393
Length = 1063
Score = 40.4 bits (93), Expect = 4e-04
Identities = 29/97 (29%), Positives = 51/97 (51%), Gaps = 4/97 (4%)
Frame = +1
Query: 145 ERRRREKLSQRFISLSSLL-PGLK-KMDKATILEDAIKHLKQLNERVKTLEE--HVADKK 200
E+ RR+KL+ +F+ L S+L PG K DKA IL DA++ + QL + +++ +K
Sbjct: 445 EKLRRDKLNDKFVELGSILEPGRPPKTDKAAILIDAVRMVTQLRGEAQKMKDTNMGLQEK 624
Query: 201 VESAVFMKRSILFEEDDRSSCDENSDQSLSKIEARVS 237
++ K + E+ + E +Q L + A+ S
Sbjct: 625 IKELKTEKNELRDEKQRLKTEKERLEQQLKSMNAQPS 735
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.312 0.128 0.346
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,212,304
Number of Sequences: 28460
Number of extensions: 48708
Number of successful extensions: 301
Number of sequences better than 10.0: 68
Number of HSP's better than 10.0 without gapping: 296
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 296
length of query: 295
length of database: 4,897,600
effective HSP length: 90
effective length of query: 205
effective length of database: 2,336,200
effective search space: 478921000
effective search space used: 478921000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 55 (25.8 bits)
Medicago: description of AC141862.23