BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC141436.3 + phase: 0 /pseudo
(223 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BF177509 65 9e-12
BP030148 48 1e-06
AV766619 35 0.014
TC16386 32 0.088
BP035375 28 0.97
AU252239 28 1.7
TC9726 weakly similar to UP|Q93Y18 (Q93Y18) SNF1 related protein... 27 3.7
TC17881 27 3.7
TC10356 similar to GB|AAM62506.1|21553413|AY085274 VAMP (vesicle... 27 3.7
AV777422 26 6.3
NP459575 NADH dehydrogenase subunit [Lotus japonicus] 25 8.2
>BF177509
Length = 472
Score = 65.1 bits (157), Expect = 9e-12
Identities = 41/162 (25%), Positives = 79/162 (48%)
Frame = +1
Query: 42 WVDNIPKQKWNQSHDDGRRWGHMTSNLVESQNNVYKGIRGLPITAIVKASYYRLAALFAK 101
W+ IP + W ++ +G+R+GH+T+N+VES N GLPI +++ +L F +
Sbjct: 19 WIRRIPPRLWATAYFEGQRFGHLTANIVESLNTWILEASGLPIIQMMECIRRQLMTWFNE 198
Query: 102 RGHEAAARVNSGEPFSENSMKYLRNEVIKSNSHHVTQFDRDRYTFSVRETIEHKEGLPKG 161
R + + P +E + + + ++ ++ V + + + E I H +G
Sbjct: 199 RRETSMQWASILVPSAE---RCVAEALERARTYQVLRANEAEF-----EVISH-----EG 339
Query: 162 EYKVDLQNKWCDCGRFRALHLPCSHVIAACSSFSHDYKTFVD 203
VD++N+ C C ++ LPC+H +AA S + F +
Sbjct: 340 SNIVDIRNRCCLCRGWQLYGLPCAHAVAALLSCRQNVHRFTE 465
>BP030148
Length = 398
Score = 48.1 bits (113), Expect = 1e-06
Identities = 22/72 (30%), Positives = 40/72 (55%)
Frame = +3
Query: 18 YAMNIPTFEYYRSEIDVADRKALAWVDNIPKQKWNQSHDDGRRWGHMTSNLVESQNNVYK 77
+ + + FE EI+ + A W+ IP + W ++ +G+R+GH+T+N+VES +
Sbjct: 177 HVLTVIEFESKILEIEEISQDAAYWIRRIPPRLWATAYFEGQRFGHLTANIVESLDTWIL 356
Query: 78 GIRGLPITAIVK 89
GLPI + +
Sbjct: 357 DASGLPIVQMTE 392
>AV766619
Length = 412
Score = 34.7 bits (78), Expect = 0.014
Identities = 20/71 (28%), Positives = 30/71 (42%)
Frame = +2
Query: 18 YAMNIPTFEYYRSEIDVADRKALAWVDNIPKQKWNQSHDDGRRWGHMTSNLVESQNNVYK 77
YA TFE + +A WV + W + +G R+ H+TSN + N
Sbjct: 197 YAPKQETFERSVENMKGISSEAFNWVMQNEPEHWANAFFNGARYNHLTSNFGQQFYNWMS 376
Query: 78 GIRGLPITAIV 88
LPIT ++
Sbjct: 377 EAHELPITQMI 409
>TC16386
Length = 619
Score = 32.0 bits (71), Expect = 0.088
Identities = 19/60 (31%), Positives = 25/60 (41%), Gaps = 3/60 (5%)
Frame = -1
Query: 167 LQNKWCDCG---RFRALHLPCSHVIAACSSFSHDYKTFVDNKFTNECVYAVYNIHFDVVH 223
L KW C R A+H P SH I +C F K+ N+C + +Y D H
Sbjct: 226 LSIKWN*CTHHFRKEAVHAPFSHKITSCFPEKQQILIFPGQKYQNDCQWNLYMYLQDTHH 47
>BP035375
Length = 543
Score = 28.5 bits (62), Expect = 0.97
Identities = 14/30 (46%), Positives = 18/30 (59%)
Frame = +1
Query: 99 FAKRGHEAAARVNSGEPFSENSMKYLRNEV 128
FA RG+ A V S PF N ++ LRNE+
Sbjct: 100 FASRGYAEVAAVKS--PFESNILRVLRNEI 183
>AU252239
Length = 417
Score = 27.7 bits (60), Expect = 1.7
Identities = 17/37 (45%), Positives = 21/37 (55%), Gaps = 1/37 (2%)
Frame = +1
Query: 136 VTQFDRDRYTFSVRETIEH-KEGLPKGEYKVDLQNKW 171
V F RDR ++ E IEH KEGL G D Q++W
Sbjct: 259 VANFCRDRMHLALAEEIEHVKEGLLIGSIN-DCQDQW 366
>TC9726 weakly similar to UP|Q93Y18 (Q93Y18) SNF1 related protein kinase,
partial (6%)
Length = 641
Score = 26.6 bits (57), Expect = 3.7
Identities = 8/13 (61%), Positives = 10/13 (76%)
Frame = +2
Query: 163 YKVDLQNKWCDCG 175
++ DL NKWC CG
Sbjct: 98 FESDLYNKWCSCG 136
>TC17881
Length = 785
Score = 26.6 bits (57), Expect = 3.7
Identities = 24/106 (22%), Positives = 41/106 (38%)
Frame = +3
Query: 73 NNVYKGIRGLPITAIVKASYYRLAALFAKRGHEAAARVNSGEPFSENSMKYLRNEVIKSN 132
++V G+ G +T + + G AA GEP + +KY+ E+
Sbjct: 183 SDVMIGVHGAAMTHFMFLKSGSVFIQVVPLGTNWAAETYYGEPARKLGLKYIGYEIHPRE 362
Query: 133 SHHVTQFDRDRYTFSVRETIEHKEGLPKGEYKVDLQNKWCDCGRFR 178
S ++D++ +I K + +D QN D RFR
Sbjct: 363 SSLYEKYDKNDPILRDPASINEKGWEYTKKIYLDSQNVKLDLRRFR 500
>TC10356 similar to GB|AAM62506.1|21553413|AY085274 VAMP (vesicle-associated
membrane protein)-associated protein-like {Arabidopsis
thaliana;}, partial (16%)
Length = 434
Score = 26.6 bits (57), Expect = 3.7
Identities = 11/24 (45%), Positives = 15/24 (61%), Gaps = 2/24 (8%)
Frame = -1
Query: 46 IPKQKWNQSHDDGR--RWGHMTSN 67
I + +W+ SHD+GR WG M N
Sbjct: 341 INRNEWSCSHDEGRMGNWGEMGMN 270
>AV777422
Length = 555
Score = 25.8 bits (55), Expect = 6.3
Identities = 14/58 (24%), Positives = 25/58 (42%)
Frame = +3
Query: 127 EVIKSNSHHVTQFDRDRYTFSVRETIEHKEGLPKGEYKVDLQNKWCDCGRFRALHLPC 184
E K ++ F+ + + + I+ KE E+K+ + N W + F L PC
Sbjct: 189 ERYKDVDDYIATFEPLIFEEAKSQIIKDKEDDEVTEWKLGVVNSWNEADDFHFLEFPC 362
>NP459575 NADH dehydrogenase subunit [Lotus japonicus]
Length = 486
Score = 25.4 bits (54), Expect = 8.2
Identities = 9/39 (23%), Positives = 22/39 (56%)
Frame = -3
Query: 33 DVADRKALAWVDNIPKQKWNQSHDDGRRWGHMTSNLVES 71
++ + + L WV+ + + +N HDD + H+ +L+ +
Sbjct: 466 ELYNHQLLTWVNGLKQFDYNSIHDDHKPKVHILPSLINN 350
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.417
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,900,883
Number of Sequences: 28460
Number of extensions: 52683
Number of successful extensions: 256
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 255
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 255
length of query: 223
length of database: 4,897,600
effective HSP length: 87
effective length of query: 136
effective length of database: 2,421,580
effective search space: 329334880
effective search space used: 329334880
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 53 (25.0 bits)
Medicago: description of AC141436.3