Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC141115.10 + phase: 0 /pseudo
         (173 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

AV410430                                                               87  1e-18
TC15901 homologue to UP|AAR20777 (AAR20777) At2g22125, partial (...    47  2e-08
TC17172 homologue to UP|AAR20777 (AAR20777) At2g22125, partial (...    42  2e-07
TC12266 weakly similar to GB|AAB68410.1|500677|YSCH9315 Yhr122wp...    27  2.5
TC15211 similar to UP|Q9ZRD9 (Q9ZRD9) GMFP4 (Fragment), partial ...    25  7.3
TC16306                                                                25  7.3
TC16813 homologue to UP|BPHE_COMTE (Q46373) Biphenyl dioxygenase...    25  9.5
AW164055                                                               25  9.5
TC10911                                                                25  9.5

>AV410430 
          Length = 374

 Score = 87.4 bits (215), Expect = 1e-18
 Identities = 49/75 (65%), Positives = 54/75 (71%), Gaps = 7/75 (9%)
 Frame = +3

Query: 13  MDALFLLIQGWSACPVEVSRDQSNAAAYAIPLLQNLIQFGPVLFFEKAEFI-------LV 65
           +D L LL Q WSACP EVSR QS AAA AIPLLQ LIQ GP  F EKAEF+       LV
Sbjct: 150 LDTLSLLRQAWSACPAEVSRAQSIAAADAIPLLQYLIQSGPPRFQEKAEFLLQCLPGTLV 329

Query: 66  MIVKRGNNMRQCVGN 80
           +I+KRGNNM+Q VGN
Sbjct: 330 VIIKRGNNMKQSVGN 374


>TC15901 homologue to UP|AAR20777 (AAR20777) At2g22125, partial (24%)
          Length = 606

 Score = 47.4 bits (111), Expect(2) = 2e-08
 Identities = 22/41 (53%), Positives = 28/41 (67%)
 Frame = +3

Query: 123 ADEHTLLPTSKSGQPRNLEVELKWSNKPSYAYSIKLSIPSF 163
           A E+TLLP SKSG PRNLE+E +WSNK +    + + I  F
Sbjct: 144 AGEYTLLPASKSGPPRNLEIEFQWSNKTNDTV*LGMEILDF 266



 Score = 25.4 bits (54), Expect(2) = 2e-08
 Identities = 14/25 (56%), Positives = 17/25 (68%)
 Frame = +2

Query: 98  YMVL*ECSSRTEASYLLQKQA*SGK 122
           +MV+*E S R EA+  LQK   SGK
Sbjct: 11  FMVI*ESSKRPEATDFLQK*EQSGK 85


>TC17172 homologue to UP|AAR20777 (AAR20777) At2g22125, partial (28%)
          Length = 553

 Score = 42.0 bits (97), Expect(2) = 2e-07
 Identities = 19/30 (63%), Positives = 23/30 (76%)
 Frame = +3

Query: 123 ADEHTLLPTSKSGQPRNLEVELKWSNKPSY 152
           A E+TL P SKSG  RNLE+E +WSNK S+
Sbjct: 183 AGEYTLSPESKSGPSRNLEIEFQWSNK*SW 272



 Score = 28.1 bits (61), Expect(2) = 2e-07
 Identities = 15/25 (60%), Positives = 18/25 (72%)
 Frame = +2

Query: 98  YMVL*ECSSRTEASYLLQKQA*SGK 122
           +MVL*E S R EAS+ LQK   +GK
Sbjct: 50  FMVL*ESSKRPEASHFLQK*EQNGK 124


>TC12266 weakly similar to GB|AAB68410.1|500677|YSCH9315 Yhr122wp
           {Saccharomyces cerevisiae;} , partial (27%)
          Length = 715

 Score = 26.6 bits (57), Expect = 2.5
 Identities = 14/48 (29%), Positives = 23/48 (47%)
 Frame = -2

Query: 113 LLQKQA*SGKADEHTLLPTSKSGQPRNLEVELKWSNKPSYAYSIKLSI 160
           ++Q Q  S K  +    PTS+ G  +   V L W  K S+ + ++  I
Sbjct: 609 IIQDQCSSNKKHDK*S*PTSRKGTKKTPTVLLHWKEKSSFNHKMEQGI 466


>TC15211 similar to UP|Q9ZRD9 (Q9ZRD9) GMFP4 (Fragment), partial (94%)
          Length = 531

 Score = 25.0 bits (53), Expect = 7.3
 Identities = 11/22 (50%), Positives = 14/22 (63%), Gaps = 1/22 (4%)
 Frame = -3

Query: 4   QWHVKKLSWMDALFL-LIQGWS 24
           QW    L W+D LF+ +I GWS
Sbjct: 118 QWLPSLLPWIDELFITIIIGWS 53


>TC16306 
          Length = 598

 Score = 25.0 bits (53), Expect = 7.3
 Identities = 12/26 (46%), Positives = 16/26 (61%)
 Frame = -3

Query: 148 NKPSYAYSIKLSIPSFKFDYLFILVL 173
           N P     +KL +PS KFD+L +L L
Sbjct: 200 NDPMADKLLKLLLPSLKFDFLDLLGL 123


>TC16813 homologue to UP|BPHE_COMTE (Q46373) Biphenyl dioxygenase beta
           subunit  (Biphenyl 2,3-dioxygenase) , partial (8%)
          Length = 621

 Score = 24.6 bits (52), Expect = 9.5
 Identities = 9/20 (45%), Positives = 13/20 (65%)
 Frame = +2

Query: 132 SKSGQPRNLEVELKWSNKPS 151
           +  G  R LE+E+ WSN+ S
Sbjct: 62  NNDGSSRTLEIEIVWSNRIS 121


>AW164055 
          Length = 361

 Score = 24.6 bits (52), Expect = 9.5
 Identities = 11/24 (45%), Positives = 15/24 (61%)
 Frame = -2

Query: 62  FILVMIVKRGNNMRQCVGNQGKIT 85
           F+L+ I+ RGN +     NQG IT
Sbjct: 282 FLLLSIMYRGNKISSIKENQGTIT 211


>TC10911 
          Length = 498

 Score = 24.6 bits (52), Expect = 9.5
 Identities = 9/28 (32%), Positives = 18/28 (64%)
 Frame = +1

Query: 144 LKWSNKPSYAYSIKLSIPSFKFDYLFIL 171
           + WS+  SYA+ I  + P+ +  Y++I+
Sbjct: 1   ITWSSPKSYAWHILEAYPTHRMPYVWIV 84


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.328    0.139    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,076,736
Number of Sequences: 28460
Number of extensions: 36868
Number of successful extensions: 229
Number of sequences better than 10.0: 18
Number of HSP's better than 10.0 without gapping: 228
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 228
length of query: 173
length of database: 4,897,600
effective HSP length: 84
effective length of query: 89
effective length of database: 2,506,960
effective search space: 223119440
effective search space used: 223119440
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 52 (24.6 bits)


Medicago: description of AC141115.10