BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140916.2 + phase: 0
(413 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19973 homologue to UP|HEM2_SOYBN (P43210) Delta-aminolevulinic... 283 2e-99
TC10021 homologue to UP|HEM2_PEA (P30124) Delta-aminolevulinic a... 209 7e-55
AV408589 183 5e-47
AV765484 92 2e-19
TC14277 similar to UP|Q9SHY3 (Q9SHY3) F1E22.9 (AT1G65720/F1E22_1... 38 0.003
BP036228 35 0.030
BI420376 34 0.039
AV409862 33 0.11
TC14969 similar to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase (cl... 33 0.11
AV419448 32 0.19
TC14545 similar to UP|Q9FT78 (Q9FT78) P23 co-chaperone, partial ... 32 0.19
TC7947 similar to UP|Q9ST69 (Q9ST69) Ribosomal protein S5, parti... 32 0.19
BP039014 31 0.33
AV426647 31 0.33
AV407543 31 0.43
TC9845 31 0.43
BP044699 30 0.56
TC10662 homologue to UP|CWP1_YEAST (P28319) Cell wall protein CW... 30 0.56
AV427009 30 0.56
AV409241 30 0.56
>TC19973 homologue to UP|HEM2_SOYBN (P43210) Delta-aminolevulinic acid
dehydratase, chloroplast precursor (Porphobilinogen
synthase) (ALADH) , partial (46%)
Length = 580
Score = 283 bits (725), Expect(2) = 2e-99
Identities = 137/146 (93%), Positives = 142/146 (96%)
Frame = +2
Query: 125 EDTPIGAMPGCYRLGWRHGLIEEVAKARDVGVNSVVLFPKIPDALKTPTGDEAYNENGLV 184
+DTPIGAMPGCYRLGWRHGL++EVAKARDVGVNSVVLFPKIPDALKTPTGDEAY+ENGLV
Sbjct: 11 KDTPIGAMPGCYRLGWRHGLVQEVAKARDVGVNSVVLFPKIPDALKTPTGDEAYSENGLV 190
Query: 185 PRTIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGVIMNDETVHQLCKQAVAQARAG 244
PR IRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGVIMNDETVHQLCKQAVAQARAG
Sbjct: 191 PRAIRLLKDKYPDLVIYTDVALDPYSSDGHDGIVREDGVIMNDETVHQLCKQAVAQARAG 370
Query: 245 ADVVSPSDMMDGRVGAMRAALDAEGF 270
ADVVSP DMMDGRV A+RAALDAE F
Sbjct: 371 ADVVSPXDMMDGRVXAIRAALDAEAF 448
Score = 95.9 bits (237), Expect(2) = 2e-99
Identities = 44/45 (97%), Positives = 44/45 (97%)
Frame = +1
Query: 270 FQHVSIMSYTAKYASSFYGPFREALDSNPRFGDKKTYQMNPANYR 314
FQHVSIMSYTAKYASSFYGPF EALDSNPRFGDKKTYQMNPANYR
Sbjct: 445 FQHVSIMSYTAKYASSFYGPFXEALDSNPRFGDKKTYQMNPANYR 579
>TC10021 homologue to UP|HEM2_PEA (P30124) Delta-aminolevulinic acid
dehydratase, chloroplast precursor (Porphobilinogen
synthase) (ALADH) (Fragment) , partial (27%)
Length = 562
Score = 209 bits (532), Expect = 7e-55
Identities = 104/109 (95%), Positives = 107/109 (97%)
Frame = +3
Query: 305 TYQMNPANYREALTEMREDETEGADILLVKPGLPYLDIIRLLRDNSPLPIAAYQVSGEYS 364
TYQMNPANYREALTEMREDE+EGADILLVKP LPYLD+IRLLRDNSPLPIAAYQVSGEYS
Sbjct: 3 TYQMNPANYREALTEMREDESEGADILLVKPALPYLDVIRLLRDNSPLPIAAYQVSGEYS 182
Query: 365 MIKAGGALKMIDEEKVMMESLLCLRRAGADIILTYFALQAARCLCGEKR 413
MIKAGGALKMIDEEKVMMESLLCLRRAGAD+ILTYFALQAAR LCGEKR
Sbjct: 183 MIKAGGALKMIDEEKVMMESLLCLRRAGADVILTYFALQAARSLCGEKR 329
>AV408589
Length = 424
Score = 183 bits (464), Expect = 5e-47
Identities = 93/131 (70%), Positives = 102/131 (76%)
Frame = +3
Query: 4 SSTIPNTPLTLNSHTYVDLKPALPLKNYLSFSSSKRRPPCLFTVRASDADFEAAVVAGNV 63
+STI N P TLN+ TYV L+P N+ S RPP LF VRASD+D EAAVVAG V
Sbjct: 45 ASTISNAPCTLNTQTYVALRPPHGTPNFAS-----PRPPRLFAVRASDSDCEAAVVAGRV 209
Query: 64 PDAPPVPPTPAAPAGTPVVTSLPIQRRPRRNRRSATHRSAFQETTLSPANFVYPLFIHEG 123
PDAPPVPP PAAPAGTPVV LP+ RRPRRNRR+ RSAFQETT+SP +FVYPLFIHEG
Sbjct: 210 PDAPPVPPKPAAPAGTPVVPPLPLHRRPRRNRRTPALRSAFQETTISPGHFVYPLFIHEG 389
Query: 124 EEDTPIGAMPG 134
EEDTPIGAMPG
Sbjct: 390 EEDTPIGAMPG 422
>AV765484
Length = 532
Score = 91.7 bits (226), Expect = 2e-19
Identities = 52/93 (55%), Positives = 56/93 (59%), Gaps = 36/93 (38%)
Frame = +2
Query: 303 KKTYQMNPANYREALTEMREDETEGADILL------------------------------ 332
K++YQMNPANYREALTEMREDE+EGADILL
Sbjct: 38 KQSYQMNPANYREALTEMREDESEGADILLVGLALSLC*SWLRMNFISCSFLNSISTQTI 217
Query: 333 ------VKPGLPYLDIIRLLRDNSPLPIAAYQV 359
VKP LPYLD+IRLLRDNSPLPIAAYQV
Sbjct: 218 EKLDLQVKPALPYLDVIRLLRDNSPLPIAAYQV 316
>TC14277 similar to UP|Q9SHY3 (Q9SHY3) F1E22.9 (AT1G65720/F1E22_13), partial
(33%)
Length = 941
Score = 37.7 bits (86), Expect = 0.003
Identities = 32/107 (29%), Positives = 47/107 (43%), Gaps = 7/107 (6%)
Frame = +3
Query: 3 SSSTIPNTPLTLNSHTYVDLKPALPLKNYLSFSSSKRRPPCLFTVRASDADFEAAVVAGN 62
SSST P T ++ L A P + +++ PP + R S A + A +
Sbjct: 267 SSSTTPKTTTNKSNPNTARLSHAAP*GSPPLLTTTS--PPSVTAPRTSSASRLPSSSALD 440
Query: 63 VPDAPPVPPTPAAPAGTPVVTSL-------PIQRRPRRNRRSATHRS 102
+PP+P T + P+ PV T+ P+ RR RR AT RS
Sbjct: 441 AALSPPLPCTSSGPSSPPVTTTAPPCTAISPMTRRSTAPRRPATSRS 581
>BP036228
Length = 626
Score = 34.7 bits (78), Expect = 0.030
Identities = 30/80 (37%), Positives = 41/80 (50%), Gaps = 2/80 (2%)
Frame = +3
Query: 24 PALPLKNYLSFSSSKRRPPCLFTVRASDADFEAAV--VAGNVPDAPPVPPTPAAPAGTPV 81
PALP F +S+ PP L T A A + + + +P P+ P+AP+ TP+
Sbjct: 135 PALPRG---IFPASQPLPPLLSTGTALTAPPPSPISMLPLLLPPPAPISSPPSAPS-TPM 302
Query: 82 VTSLPIQRRPRRNRRSATHR 101
SL RRPRRNR S+ R
Sbjct: 303 PPSLT-NRRPRRNRNSSAAR 359
>BI420376
Length = 562
Score = 34.3 bits (77), Expect = 0.039
Identities = 29/92 (31%), Positives = 43/92 (46%)
Frame = +2
Query: 10 TPLTLNSHTYVDLKPALPLKNYLSFSSSKRRPPCLFTVRASDADFEAAVVAGNVPDAPPV 69
T L+L S T + P + ++++ PP S A + ++P PP+
Sbjct: 80 TSLSLGSTTLPSPSFSAPSSTSSASGNTQQTPP----PPPSAATSPPSTAPTSIPPPPPL 247
Query: 70 PPTPAAPAGTPVVTSLPIQRRPRRNRRSATHR 101
PPTP+ +P T+ P RRPRR R S T R
Sbjct: 248 PPTPSI---SPPATTTP-TRRPRR-RVSPTRR 328
>AV409862
Length = 420
Score = 32.7 bits (73), Expect = 0.11
Identities = 25/83 (30%), Positives = 36/83 (43%), Gaps = 7/83 (8%)
Frame = +3
Query: 35 SSSKRRPPCLFTVRASDADFEAAVVAG--NVPDAPPVPPTPAAPAGTPV-----VTSLPI 87
S SK PP T A+ + + A + P A PPT P+ T + ++L
Sbjct: 150 SPSKPEPPATSTPSATSSTSASKTAASTPSTPSASSPPPTSPPPSSTTLFAPSLASTLAS 329
Query: 88 QRRPRRNRRSATHRSAFQETTLS 110
+ R R N S + S+ TTLS
Sbjct: 330 RARARSNPSSRAYASSNAPTTLS 398
>TC14969 similar to PIR|PQ0772|PQ0772 4-coumarate-CoA ligase (clone
GM4CL1B) - soybean (fragment) {Glycine
max;} , partial (70%)
Length = 928
Score = 32.7 bits (73), Expect = 0.11
Identities = 34/107 (31%), Positives = 45/107 (41%), Gaps = 4/107 (3%)
Frame = -3
Query: 23 KPALPLKNYLSFSSSKRRPPCLFTVRASDADFEAAVVAGNVPDAPPVPPTP----AAPAG 78
+ A PL +SS + PP SD+ E A+ A APP PPTP +APA
Sbjct: 590 REAQPLTAPTLWSSGQTPPP-------SDSSPEPALAA-----APPWPPTPCASESAPAT 447
Query: 79 TPVVTSLPIQRRPRRNRRSATHRSAFQETTLSPANFVYPLFIHEGEE 125
T TS R + R SA + T +PLF +G +
Sbjct: 446 TLSQTSASEAHRSPLSARREERCSARKCETGEARKAEHPLFPEKGSD 306
>AV419448
Length = 382
Score = 32.0 bits (71), Expect = 0.19
Identities = 29/98 (29%), Positives = 42/98 (42%), Gaps = 2/98 (2%)
Frame = +2
Query: 2 ASSSTIPNTPLTLNSHTYVDLKPALPLKNYLSFSSSKRRPPCLFTVRASDADFEAAVVAG 61
ASS T + P +S T P PL LS +S T +S + + +
Sbjct: 119 ASSWTSASAPPP-SSPTATPSAPTPPLSAALSLAS---------TATSSPSPSPISNPSA 268
Query: 62 NVPDAPPVPPT--PAAPAGTPVVTSLPIQRRPRRNRRS 97
++P PP PPT P++ + +P TS P R RS
Sbjct: 269 SLPPTPPPPPTKTPSSTSSSPAATSSPAARVDPTRARS 382
>TC14545 similar to UP|Q9FT78 (Q9FT78) P23 co-chaperone, partial (46%)
Length = 1127
Score = 32.0 bits (71), Expect = 0.19
Identities = 16/52 (30%), Positives = 24/52 (45%)
Frame = -2
Query: 63 VPDAPPVPPTPAAPAGTPVVTSLPIQRRPRRNRRSATHRSAFQETTLSPANF 114
+P PP+PP+P P P+ ++ SAT S+ T LS + F
Sbjct: 565 IPPIPPIPPSPPRPPSPPIPIPPNFEKSIPPISGSATTSSSSSSTHLSQSTF 410
>TC7947 similar to UP|Q9ST69 (Q9ST69) Ribosomal protein S5, partial (81%)
Length = 1294
Score = 32.0 bits (71), Expect = 0.19
Identities = 14/48 (29%), Positives = 26/48 (54%), Gaps = 2/48 (4%)
Frame = +2
Query: 64 PDAPPVPPTPAAPAGTPVVTSLPIQRRPRRNRRS--ATHRSAFQETTL 109
P +PP PP P+ P P T P P+ +++ +TH+ + + ++L
Sbjct: 221 PSSPPPPPPPSPPNANPPTTLTPPSSTPQTQKKTSPSTHQKSPKASSL 364
>BP039014
Length = 566
Score = 31.2 bits (69), Expect = 0.33
Identities = 14/27 (51%), Positives = 18/27 (65%), Gaps = 1/27 (3%)
Frame = +2
Query: 67 PPVPPTPAAPAGTPVVT-SLPIQRRPR 92
PP PPTPAAP P T + P +R+P+
Sbjct: 92 PPHPPTPAAPIPPPTTTIAAPEKRKPK 172
>AV426647
Length = 430
Score = 31.2 bits (69), Expect = 0.33
Identities = 14/27 (51%), Positives = 18/27 (65%), Gaps = 1/27 (3%)
Frame = +1
Query: 67 PPVPPTPAAPAGTPVVT-SLPIQRRPR 92
PP PPTPAAP P T + P +R+P+
Sbjct: 109 PPHPPTPAAPIPPPTTTIAAPEKRKPK 189
>AV407543
Length = 227
Score = 30.8 bits (68), Expect = 0.43
Identities = 19/49 (38%), Positives = 26/49 (52%)
Frame = +1
Query: 2 ASSSTIPNTPLTLNSHTYVDLKPALPLKNYLSFSSSKRRPPCLFTVRAS 50
AS+ST N L+ DL+P++ N+LSF+ RR P F AS
Sbjct: 85 ASTSTAANCKLSS------DLRPSVSFNNFLSFNYIPRRCPSRFRASAS 213
>TC9845
Length = 614
Score = 30.8 bits (68), Expect = 0.43
Identities = 19/70 (27%), Positives = 33/70 (47%)
Frame = +2
Query: 5 STIPNTPLTLNSHTYVDLKPALPLKNYLSFSSSKRRPPCLFTVRASDADFEAAVVAGNVP 64
STI +T L ++H+ P + ++NY+ S + L+ SD E ++
Sbjct: 86 STISSTTLLESAHSV----PPMRIENYMPSSGTS----FLYATDLSDGKSECLFEGWDIV 241
Query: 65 DAPPVPPTPA 74
+ PP PP P+
Sbjct: 242 ERPPFPPPPS 271
>BP044699
Length = 522
Score = 30.4 bits (67), Expect = 0.56
Identities = 18/51 (35%), Positives = 25/51 (48%)
Frame = -3
Query: 68 PVPPTPAAPAGTPVVTSLPIQRRPRRNRRSATHRSAFQETTLSPANFVYPL 118
P+PP P+ A +PV R RR S ++RS T L P + + PL
Sbjct: 277 PLPPDPSRRAASPVAF------RRRRKPPSRSNRSCSNTTCLKPRSHLSPL 143
>TC10662 homologue to UP|CWP1_YEAST (P28319) Cell wall protein CWP1
precursor, partial (6%)
Length = 937
Score = 30.4 bits (67), Expect = 0.56
Identities = 28/92 (30%), Positives = 41/92 (44%), Gaps = 1/92 (1%)
Frame = +2
Query: 3 SSSTIPNTPLTLNSHTYVDLKP-ALPLKNYLSFSSSKRRPPCLFTVRASDADFEAAVVAG 61
S ST LTL+S + P + P SF ++ P L + +S A ++
Sbjct: 98 SESTTSIASLTLSSSSSSTRSPTSKP*VAAASFPAAST-PSSLKSKTSSFASTASSPTTT 274
Query: 62 NVPDAPPVPPTPAAPAGTPVVTSLPIQRRPRR 93
++P PP P PAAP+GT +S P R
Sbjct: 275 HLPPPPP-PTNPAAPSGTSSASSSAASPNPSR 367
>AV427009
Length = 404
Score = 30.4 bits (67), Expect = 0.56
Identities = 13/40 (32%), Positives = 21/40 (52%), Gaps = 1/40 (2%)
Frame = +2
Query: 64 PDAPPVPPTPAAPAGTPVVTSLPIQRRPRRNR-RSATHRS 102
P + P+PP P PA + ++S ++ P R S HR+
Sbjct: 263 PPSSPLPPLPTTPASSSTISSTSSEKTPSSTRWSSCNHRN 382
>AV409241
Length = 282
Score = 30.4 bits (67), Expect = 0.56
Identities = 22/56 (39%), Positives = 27/56 (47%), Gaps = 8/56 (14%)
Frame = +2
Query: 64 PDAPPVPPTPA--APAGTP----VVTSLPIQRRPRRNRRSAT--HRSAFQETTLSP 111
P PP PP PA +P TP T P+ +P R ++T RSA TT SP
Sbjct: 110 PPTPPPPPPPATVSPPPTPSPPAAATPSPLSAKPSRKIPTSTTSPRSAPPPTTSSP 277
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,443,059
Number of Sequences: 28460
Number of extensions: 91837
Number of successful extensions: 1251
Number of sequences better than 10.0: 104
Number of HSP's better than 10.0 without gapping: 1009
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1164
length of query: 413
length of database: 4,897,600
effective HSP length: 93
effective length of query: 320
effective length of database: 2,250,820
effective search space: 720262400
effective search space used: 720262400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC140916.2