BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140916.14 - phase: 0 /pseudo
(541 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12443 similar to UP|Q84LR1 (Q84LR1) Root-specific metal transp... 425 e-119
BP061751 239 6e-64
TC19464 similar to UP|Q84LR1 (Q84LR1) Root-specific metal transp... 185 2e-47
BP072596 174 3e-44
TC11148 homologue to UP|Q7X9B8 (Q7X9B8) Ferrous ion membrane tra... 123 7e-29
AV408147 100 1e-21
TC13552 59 2e-09
BP066580 39 0.002
AV408212 35 0.024
AV411486 32 0.34
AV415922 31 0.58
TC10871 similar to UP|Q7X9B8 (Q7X9B8) Ferrous ion membrane trans... 31 0.58
CB826726 30 1.3
AW719943 29 1.7
AV426390 29 1.7
TC16562 similar to GB|AAM78073.1|21928089|AY125563 AT4g24660/F22... 29 1.7
AV780096 29 2.2
BP049929 29 2.2
TC8703 similar to UP|BAC97801 (BAC97801) RelA-SpoT like protein ... 28 2.9
BP059696 28 2.9
>TC12443 similar to UP|Q84LR1 (Q84LR1) Root-specific metal transporter,
partial (42%)
Length = 790
Score = 425 bits (1092), Expect = e-119
Identities = 215/258 (83%), Positives = 235/258 (90%)
Frame = +2
Query: 274 GFALFVAFLINVAMISVSGTVCSANDLSGDNVERCNDLTLNSASFLLKNVLGRSSSTIYA 333
GFALFVAFLINVAMISV+GTVC AN+LS +N +RCNDLTLNSASFLLKN LGRSSST+YA
Sbjct: 17 GFALFVAFLINVAMISVTGTVCFANNLSAENTDRCNDLTLNSASFLLKNALGRSSSTVYA 196
Query: 334 IALLASGQSSTITGTYAGQYIMQGFLDIRMKRWKRNLMTRCIAIAPSLAVAIIGGSSGSS 393
IALLASGQSSTITGTYAGQYIMQGFL+I+MKRWKRNLMTRCIAIAPSL V+IIGGSSGSS
Sbjct: 197 IALLASGQSSTITGTYAGQYIMQGFLEIKMKRWKRNLMTRCIAIAPSLIVSIIGGSSGSS 376
Query: 394 RLIIIASMILSFELPFALIPLLKFSSSSTKMGPHKNSMIIITISWILGFGIISINVYYLS 453
RLIIIASMILSFELPFALIPLLKFS SSTKMGPHK+S+III ISWILG GII NVY+LS
Sbjct: 377 RLIIIASMILSFELPFALIPLLKFSCSSTKMGPHKSSLIIIIISWILGLGIIGANVYFLS 556
Query: 454 TAFVKWIIHSSLPKVANVFIGIIVFPLMAIYIASVIYLTFRKDTVEMFIETKNDTVMQNQ 513
T+FVKW+IHSSLPKVANVFIG IVFPLMA+YI S IYLTFRKDTV+++ ETKN +Q
Sbjct: 557 TSFVKWLIHSSLPKVANVFIGTIVFPLMAVYIISTIYLTFRKDTVKIYTETKNIDTVQRH 736
Query: 514 VEKGIVDNGQLELSHVPY 531
VEKG +DN QLEL + PY
Sbjct: 737 VEKGTLDNDQLELRNAPY 790
>BP061751
Length = 501
Score = 239 bits (611), Expect = 6e-64
Identities = 120/145 (82%), Positives = 134/145 (91%)
Frame = -2
Query: 289 SVSGTVCSANDLSGDNVERCNDLTLNSASFLLKNVLGRSSSTIYAIALLASGQSSTITGT 348
SV+GTVC + +LS ++ + CNDL LNSASFLLKN LGRSSST+YAIALLASGQSSTITGT
Sbjct: 500 SVTGTVCFSKNLSAEDTDLCNDLNLNSASFLLKNTLGRSSSTVYAIALLASGQSSTITGT 321
Query: 349 YAGQYIMQGFLDIRMKRWKRNLMTRCIAIAPSLAVAIIGGSSGSSRLIIIASMILSFELP 408
YAGQYIMQGFL+I+MKRWKRNLMTRCI IAPSL V+IIGGSSGS RLIIIASMILSFELP
Sbjct: 320 YAGQYIMQGFLEIKMKRWKRNLMTRCICIAPSLIVSIIGGSSGSGRLIIIASMILSFELP 141
Query: 409 FALIPLLKFSSSSTKMGPHKNSMII 433
FALIPLLKFS SSTKMGPH++S+I+
Sbjct: 140 FALIPLLKFSCSSTKMGPHRSSLIV 66
>TC19464 similar to UP|Q84LR1 (Q84LR1) Root-specific metal transporter,
partial (22%)
Length = 503
Score = 185 bits (469), Expect = 2e-47
Identities = 91/111 (81%), Positives = 101/111 (90%)
Frame = +3
Query: 60 LFVISQFIVETDLQAGANHGYELLWLVLIGLIFALIIQSLAANLGVTTGKHLSEVCEVEY 119
+F F VETDLQAGA+H YELLWLVL+GLIFALIIQSLAANLGV TGKHLSEVC++EY
Sbjct: 171 IFDFRLFAVETDLQAGASHRYELLWLVLVGLIFALIIQSLAANLGVITGKHLSEVCKIEY 350
Query: 120 PLFVKYCLWLLAEVAVIAADIPEVIGTAFALNILFNIPLWAGVLLTGFSTL 170
PL VKYCLWLLAEVAVIA+DIPEVIGTAF+LNILF+IPLW GVL++G STL
Sbjct: 351 PLSVKYCLWLLAEVAVIASDIPEVIGTAFSLNILFHIPLWVGVLMSGCSTL 503
>BP072596
Length = 382
Score = 174 bits (441), Expect = 3e-44
Identities = 84/97 (86%), Positives = 93/97 (95%)
Frame = +3
Query: 68 VETDLQAGANHGYELLWLVLIGLIFALIIQSLAANLGVTTGKHLSEVCEVEYPLFVKYCL 127
+ETDLQAGA+H YELLWLVL+GLIFALIIQSLAANLGV TGKHLSEVC++EYPL VKYCL
Sbjct: 90 METDLQAGASHRYELLWLVLVGLIFALIIQSLAANLGVITGKHLSEVCKIEYPLSVKYCL 269
Query: 128 WLLAEVAVIAADIPEVIGTAFALNILFNIPLWAGVLL 164
WLLAEVAVIA+DIPEVIGTAF+LNILF+IPLW GVL+
Sbjct: 270 WLLAEVAVIASDIPEVIGTAFSLNILFHIPLWVGVLM 380
>TC11148 homologue to UP|Q7X9B8 (Q7X9B8) Ferrous ion membrane transport
protein DMT1, partial (33%)
Length = 622
Score = 123 bits (309), Expect = 7e-29
Identities = 62/119 (52%), Positives = 83/119 (69%), Gaps = 2/119 (1%)
Frame = +1
Query: 68 VETDLQAGANHGYELLWLVLIGLIFALIIQSLAANLGVTTGKHLSEVCEVEYPLFVKYCL 127
+E DLQAGA GY LLWL++ L+IQ L+A LGV TGKHL+E+C EYP + + L
Sbjct: 259 LEGDLQAGAIAGYSLLWLLMWATGMGLLIQLLSARLGVATGKHLAELCREEYPTWARMVL 438
Query: 128 WLLAEVAVIAADIPEVIGTAFALNILFN--IPLWAGVLLTGFSTLLLLSLQRFGVRKLE 184
W++AEVA+I +DI EVIG+A A+ IL N +PLWA V++T + L L+ +GVR LE
Sbjct: 439 WVMAEVALIGSDIQEVIGSAIAIRILSNGVVPLWAEVVITALDWFIFLFLENYGVRSLE 615
>AV408147
Length = 427
Score = 99.8 bits (247), Expect = 1e-21
Identities = 64/149 (42%), Positives = 91/149 (60%), Gaps = 4/149 (2%)
Frame = +1
Query: 218 VPKLAGEGAVADAIALLGALIMPHNLFLHSALVLSRKVPKSVRG-INEACRYFLYESGFA 276
VPKL+ + A+ ++G LIMP+N+FLHSALV SRK+ S +G + EA Y+ ES A
Sbjct: 4 VPKLSSR-TIQQAVGVVGCLIMPYNVFLHSALVQSRKIDHSNKGRVQEALNYYSIESTLA 180
Query: 277 LFVAFLINVAMISVSGTVCSANDLSGDNVERCNDLTLNSASFLLKNVLGRSSSTI---YA 333
L V+F+IN+ + +V A G V + + L +A L++ G I +
Sbjct: 181 LVVSFVINIFVTTVF-----AKGFYGSEV--ADSIGLVNAGQYLQDAYGGGVVPILYIWG 339
Query: 334 IALLASGQSSTITGTYAGQYIMQGFLDIR 362
I LLA+GQSSTI GTYAGQ+IM GFL+++
Sbjct: 340 IGLLAAGQSSTIAGTYAGQFIMGGFLNLK 426
>TC13552
Length = 486
Score = 59.3 bits (142), Expect = 2e-09
Identities = 27/35 (77%), Positives = 30/35 (85%)
Frame = +2
Query: 507 DTVMQNQVEKGIVDNGQLELSHVPYREDLADIPLP 541
D +MQ VEKG ++NGQLELS VPYREDLADIPLP
Sbjct: 2 DPMMQTHVEKGFMNNGQLELSQVPYREDLADIPLP 106
>BP066580
Length = 330
Score = 39.3 bits (90), Expect = 0.002
Identities = 17/26 (65%), Positives = 20/26 (76%)
Frame = -1
Query: 516 KGIVDNGQLELSHVPYREDLADIPLP 541
KG +DN L+L + PYREDLA IPLP
Sbjct: 273 KGTLDNDLLDLRNAPYREDLASIPLP 196
>AV408212
Length = 362
Score = 35.4 bits (80), Expect = 0.024
Identities = 16/30 (53%), Positives = 21/30 (69%)
Frame = +3
Query: 5 IQQQQQQQQQQQHQVMETSSNHEEP*SWKQ 34
+QQQQQQQQQQQ Q + S ++P S +Q
Sbjct: 87 LQQQQQQQQQQQQQQQQQQSQQQQPQSQQQ 176
>AV411486
Length = 419
Score = 31.6 bits (70), Expect = 0.34
Identities = 12/24 (50%), Positives = 20/24 (83%)
Frame = +3
Query: 5 IQQQQQQQQQQQHQVMETSSNHEE 28
++QQQQQQQQQQ Q++E ++++
Sbjct: 276 MEQQQQQQQQQQQQLVEAKHHNDD 347
>AV415922
Length = 422
Score = 30.8 bits (68), Expect = 0.58
Identities = 14/24 (58%), Positives = 17/24 (70%)
Frame = +3
Query: 6 QQQQQQQQQQQHQVMETSSNHEEP 29
QQQQQQQQQQQ Q + S++ P
Sbjct: 186 QQQQQQQQQQQQQQQQPSNSQ*SP 257
>TC10871 similar to UP|Q7X9B8 (Q7X9B8) Ferrous ion membrane transport
protein DMT1, partial (14%)
Length = 722
Score = 30.8 bits (68), Expect = 0.58
Identities = 19/63 (30%), Positives = 29/63 (45%)
Frame = +1
Query: 435 TISWILGFGIISINVYYLSTAFVKWIIHSSLPKVANVFIGIIVFPLMAIYIASVIYLTFR 494
TISW++ + IN Y L F +V +G ++ L A Y+ +IYL +R
Sbjct: 7 TISWLVAALVTMINGYLLVEFFSS--------EVNGAIVGAVLGTLTAAYVGFIIYLIWR 162
Query: 495 KDT 497
T
Sbjct: 163 AFT 171
>CB826726
Length = 505
Score = 29.6 bits (65), Expect = 1.3
Identities = 12/25 (48%), Positives = 15/25 (60%)
Frame = +2
Query: 8 QQQQQQQQQHQVMETSSNHEEP*SW 32
QQQQQQQQQH V + ++ W
Sbjct: 383 QQQQQQQQQHYVQRNLAQQQQQQQW 457
Score = 27.7 bits (60), Expect = 4.9
Identities = 13/19 (68%), Positives = 15/19 (78%)
Frame = +1
Query: 6 QQQQQQQQQQQHQVMETSS 24
QQQQQQQ+QQ QV TS+
Sbjct: 241 QQQQQQQRQQGQQVPTTSA 297
Score = 27.3 bits (59), Expect = 6.4
Identities = 12/19 (63%), Positives = 16/19 (84%)
Frame = +1
Query: 6 QQQQQQQQQQQHQVMETSS 24
+QQQQQQQ+QQ Q + T+S
Sbjct: 238 EQQQQQQQRQQGQQVPTTS 294
>AW719943
Length = 508
Score = 29.3 bits (64), Expect = 1.7
Identities = 12/13 (92%), Positives = 13/13 (99%)
Frame = +2
Query: 7 QQQQQQQQQQHQV 19
QQQQQQQQQQHQ+
Sbjct: 188 QQQQQQQQQQHQL 226
>AV426390
Length = 399
Score = 29.3 bits (64), Expect = 1.7
Identities = 18/45 (40%), Positives = 23/45 (51%), Gaps = 8/45 (17%)
Frame = +3
Query: 1 MANLIQQQQQQQQQQQHQVMETSS---NHEEP-----*SWKQDYT 37
++N QQ QQQQQQQ H + +S N P WK DY+
Sbjct: 60 LSNQQQQPQQQQQQQHHSHPDCASLIANSHSPSFPYFRDWKPDYS 194
>TC16562 similar to GB|AAM78073.1|21928089|AY125563 AT4g24660/F22K18_140
{Arabidopsis thaliana;}, partial (29%)
Length = 621
Score = 29.3 bits (64), Expect = 1.7
Identities = 15/48 (31%), Positives = 28/48 (58%)
Frame = -2
Query: 20 METSSNHEEP*SWKQDYTYVFFFHLFLIHFYVFVFVLIDRLFVISQFI 67
+++ H EP D ++ FFF FL+ F++ VFV++ +L + F+
Sbjct: 236 VKSRRRHREP---HLDLSFFFFFFFFLLLFFLEVFVVV-KLHIAIHFL 105
>AV780096
Length = 564
Score = 28.9 bits (63), Expect = 2.2
Identities = 18/76 (23%), Positives = 40/76 (51%), Gaps = 3/76 (3%)
Frame = -1
Query: 42 FHLFLIHFYVFVFVLIDRLFVISQFIVETDLQAGANHGYE---LLWLVLIGLIFALIIQS 98
F + I F + + V+ + FI +GA+HG+E +L+ +++ L+F L++
Sbjct: 240 FRISRITFIILIIVISSHSIFAAGFIRLVVGASGASHGFEFLVVLFDLVVNLLFLLLLLF 61
Query: 99 LAANLGVTTGKHLSEV 114
L + + +H+S++
Sbjct: 60 L-FGI*AVSSRHVSQI 16
>BP049929
Length = 361
Score = 28.9 bits (63), Expect = 2.2
Identities = 9/16 (56%), Positives = 13/16 (81%)
Frame = +3
Query: 40 FFFHLFLIHFYVFVFV 55
FFFH F I F++F+F+
Sbjct: 6 FFFHFFFIKFFIFIFL 53
>TC8703 similar to UP|BAC97801 (BAC97801) RelA-SpoT like protein PsRSH1,
partial (16%)
Length = 604
Score = 28.5 bits (62), Expect = 2.9
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = +1
Query: 32 WKQDYTYVFFFHLFLIHFYVFVFVLIDRLFVISQFIVETDLQAGANH 78
W + T + HLFL+H V + I L++ + ++ +L N+
Sbjct: 373 WFKKLTLAWLSHLFLLHILETVMLYISLLYITTIYVTSLNLLTRFNY 513
>BP059696
Length = 206
Score = 28.5 bits (62), Expect = 2.9
Identities = 10/23 (43%), Positives = 15/23 (64%)
Frame = +1
Query: 7 QQQQQQQQQQHQVMETSSNHEEP 29
QQQ +QQ QQH +++ H+ P
Sbjct: 133 QQQHRQQHQQHAAASSTTQHQSP 201
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.328 0.141 0.415
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,419,754
Number of Sequences: 28460
Number of extensions: 136381
Number of successful extensions: 1718
Number of sequences better than 10.0: 59
Number of HSP's better than 10.0 without gapping: 1280
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1483
length of query: 541
length of database: 4,897,600
effective HSP length: 95
effective length of query: 446
effective length of database: 2,193,900
effective search space: 978479400
effective search space used: 978479400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC140916.14