Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC140850.3 + phase: 0 /pseudo
         (650 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

BG662087                                                               82  2e-16
AV410603                                                               52  2e-07
AV771268                                                               29  2.7
TC18870 similar to UP|Q9SGU7 (Q9SGU7) F1N19.25 (At1g64680), part...    28  4.6
TC16653 similar to UP|Q8W1A1 (Q8W1A1) Adenosine 5'-phosphosulfat...    27  7.9

>BG662087 
          Length = 373

 Score = 82.4 bits (202), Expect = 2e-16
 Identities = 37/65 (56%), Positives = 49/65 (74%)
 Frame = +1

Query: 116 GKVRMCVDFRDLNKASPKDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPEDREK 175
           GK RM VD+ DLNKA PKD++PLP ID LVD  + +++ S MD +SGY+ IKM P D +K
Sbjct: 16  GKWRMWVDYTDLNKACPKDSYPLPSIDKLVDGASDNELLSLMDAYSGYHQIKMHPSDEDK 195

Query: 176 TSFIT 180
           T+F+T
Sbjct: 196 TAFMT 210


>AV410603 
          Length = 162

 Score = 52.4 bits (124), Expect = 2e-07
 Identities = 25/49 (51%), Positives = 33/49 (67%)
 Frame = +1

Query: 133 KDNFPLPHIDVLVDNTAQSKVFSFMDGFSGYNLIKMSPEDREKTSFITH 181
           KD+FP+P +D L+D    S+ FS +D  SGY+ I + PEDR KT F TH
Sbjct: 10  KDSFPMPTVDELLDELRGSQFFSKLDLRSGYHQILVKPEDRHKTVFRTH 156


>AV771268 
          Length = 514

 Score = 28.9 bits (63), Expect = 2.7
 Identities = 29/94 (30%), Positives = 42/94 (43%), Gaps = 8/94 (8%)
 Frame = -3

Query: 306 ITSPDSYLT*PQPAGRSSSYSGRISLLYGTMNAKKLLIASRITCCNHLSLS---HPWKEG 362
           IT   SY T P   G+SS +S ++ L+Y  +    L  A  I    +L L    + W E 
Sbjct: 368 ITHVLSYPTMPPS*GKSSPHSNKLILMY--ILRSFLASAFPIHYAFNLRLFVSIYFWFER 195

Query: 363 L*LCIC-----QCLMNLWDAYLVNKMRLERKNML 391
           L + +C       L+N W  Y+   +   R NML
Sbjct: 194 LMIWLCPPNEENMLINHWQHYVARPLVTSRMNML 93


>TC18870 similar to UP|Q9SGU7 (Q9SGU7) F1N19.25 (At1g64680), partial (51%)
          Length = 747

 Score = 28.1 bits (61), Expect = 4.6
 Identities = 11/25 (44%), Positives = 17/25 (68%)
 Frame = +3

Query: 547 PRRGITSLLPPGFCSNVQTIWPNTK 571
           P RG+ S+LPPG  +  + ++P TK
Sbjct: 12  PSRGLLSMLPPGAPAQFRKLFPPTK 86


>TC16653 similar to UP|Q8W1A1 (Q8W1A1) Adenosine 5'-phosphosulfate
           reductase, partial (69%)
          Length = 1242

 Score = 27.3 bits (59), Expect = 7.9
 Identities = 12/31 (38%), Positives = 18/31 (57%)
 Frame = +2

Query: 74  GLIQIWLSRLRVRFKSRLMRVFLMTVEYPEW 104
           G + I+L RLR   +S L   FLM + + +W
Sbjct: 485 GNLSIFLMRLRSTMESTLSTCFLMLLRFKDW 577


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.347    0.151    0.514 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,141,000
Number of Sequences: 28460
Number of extensions: 180784
Number of successful extensions: 1598
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1583
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1598
length of query: 650
length of database: 4,897,600
effective HSP length: 96
effective length of query: 554
effective length of database: 2,165,440
effective search space: 1199653760
effective search space used: 1199653760
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 15 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (21.7 bits)
S2: 58 (26.9 bits)


Medicago: description of AC140850.3