BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140721.4 + phase: 0
(101 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI417524 31 0.039
AW720177 31 0.039
BP081080 29 0.11
TC8330 weakly similar to GB|AAM61257.1|21536925|AY084696 calmodu... 29 0.15
TC13228 homologue to UP|Q9LNN8 (Q9LNN8) F8L10.1 protein, partial... 29 0.15
TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding p... 28 0.33
TC15655 similar to UP|Q8LE99 (Q8LE99) Transketolase-like protein... 27 0.43
TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase ... 27 0.43
BP034485 27 0.43
CB828183 27 0.56
TC9175 weakly similar to UP|INAD_DROME (Q24008) Inactivation-no-... 27 0.73
BP030058 27 0.73
TC12771 similar to UP|Q945N7 (Q945N7) AT5g04420/T32M21_20, parti... 27 0.73
TC15082 similar to GB|AAM16172.1|20334832|AY094016 At2g39720/T5I... 26 1.2
TC9308 weakly similar to UP|Q9SQ55 (Q9SQ55) Nuclear RNA binding ... 25 1.6
TC17395 similar to UP|Q9LN83 (Q9LN83) T12C24.17, partial (37%) 25 1.6
TC14308 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial ... 25 1.6
TC12507 25 2.1
TC12341 similar to GB|AAP37848.1|30725652|BT008489 At2g29650 {Ar... 25 2.8
AV419982 25 2.8
>BI417524
Length = 462
Score = 30.8 bits (68), Expect = 0.039
Identities = 17/66 (25%), Positives = 33/66 (49%)
Frame = -1
Query: 23 PGGTGGKSLEAQEHLAEGRSKGGQTRKEQLGTEGYQEMGRKGGLSTMEKSGGERAEEEGI 82
P G ++ A EH+ G GG+ +G G E+ +GG++ G+R ++G+
Sbjct: 405 PAHEGAEADIAAEHI--GDDDGGKVDVGDVGVAGVIEVFGEGGVAATGDEQGKRRGKKGL 232
Query: 83 DIDESK 88
++ E +
Sbjct: 231 EVGEER 214
>AW720177
Length = 505
Score = 30.8 bits (68), Expect = 0.039
Identities = 28/84 (33%), Positives = 44/84 (52%), Gaps = 7/84 (8%)
Frame = -3
Query: 21 VVPGGTGGKSLEAQEH----LAEGRSKGGQTRKE-QLGTE-GYQEMGRKGGLSTM-EKSG 73
V GG+GG E+ + E +GG+ E LG E +E+G GG++ + E G
Sbjct: 485 VALGGSGGVEEESTTE*YVAVGEVAVEGGEEGVEVALGLELXAREVG--GGVALLREGDG 312
Query: 74 GERAEEEGIDIDESKFKTGGGGGR 97
G + EG++ E ++ GGGGG+
Sbjct: 311 GVESGGEGVEEVEREWVGGGGGGK 240
>BP081080
Length = 458
Score = 29.3 bits (64), Expect = 0.11
Identities = 23/79 (29%), Positives = 35/79 (44%), Gaps = 1/79 (1%)
Frame = +1
Query: 23 PGGTGGKSLEAQEHLAEGRSKGGQTRKEQLGTEGYQEMGRKGGLSTMEKSGGERAEEEGI 82
P GG S ++ AEG GG+T + G+ + +K G GGE+A++ I
Sbjct: 148 PNSDGGGSPSKEDTQAEGDGDGGETTSDGDGS----DRRKKNGFLL----GGEKAKDASI 303
Query: 83 DI-DESKFKTGGGGGRSQN 100
+ E K G RS +
Sbjct: 304 QVRREEKVAACSGFSRSDD 360
>TC8330 weakly similar to GB|AAM61257.1|21536925|AY084696 calmodulin-like
protein {Arabidopsis thaliana;} , partial (72%)
Length = 1174
Score = 28.9 bits (63), Expect = 0.15
Identities = 15/49 (30%), Positives = 22/49 (44%)
Frame = -3
Query: 53 GTEGYQEMGRKGGLSTMEKSGGERAEEEGIDIDESKFKTGGGGGRSQNK 101
G G+ G E++ G E G+D+DE + GGGG R +
Sbjct: 185 GERGWVGSGEVVLFGRSEEAFGAEFEGFGVDVDELHWNNGGGG*RESGE 39
>TC13228 homologue to UP|Q9LNN8 (Q9LNN8) F8L10.1 protein, partial (11%)
Length = 421
Score = 28.9 bits (63), Expect = 0.15
Identities = 20/75 (26%), Positives = 32/75 (42%)
Frame = -3
Query: 22 VPGGTGGKSLEAQEHLAEGRSKGGQTRKEQLGTEGYQEMGRKGGLSTMEKSGGERAEEEG 81
+P G+G + L ++H A G GG+ G G +GG S + G R G
Sbjct: 398 LPPGSGSEVLRRRDHGAFGG*GGGECPSRPRGH*SGTRGGLRGG-SFCHRIGHRRRRSPG 222
Query: 82 IDIDESKFKTGGGGG 96
+ + + + GGG
Sbjct: 221 LGVFRRRLRRRLGGG 177
>TC14758 similar to UP|O24601 (O24601) Glycine-rich RNA binding protein 2,
partial (93%)
Length = 725
Score = 27.7 bits (60), Expect = 0.33
Identities = 24/73 (32%), Positives = 28/73 (37%)
Frame = +1
Query: 24 GGTGGKSLEAQEHLAEGRSKGGQTRKEQLGTEGYQEMGRKGGLSTMEKSGGERAEEEGID 83
GG GG EG GG + G G G GG GG R G D
Sbjct: 340 GGRGGGGYGGGGGRREGGYGGGGGSRGYGGGGGGYGGGGGGGY------GGRREGGYGGD 501
Query: 84 IDESKFKTGGGGG 96
S++ +GGGGG
Sbjct: 502 GGGSRYGSGGGGG 540
>TC15655 similar to UP|Q8LE99 (Q8LE99) Transketolase-like protein, partial
(17%)
Length = 575
Score = 27.3 bits (59), Expect = 0.43
Identities = 25/72 (34%), Positives = 31/72 (42%)
Frame = -2
Query: 27 GGKSLEAQEHLAEGRSKGGQTRKEQLGTEGYQEMGRKGGLSTMEKSGGERAEEEGIDIDE 86
GGK+ + + EG +GG + G EG R GG G A EEG+ E
Sbjct: 208 GGKAAVSGWSVGEGEGEGGGLAEAGEGGEG---EARGGG-------EGRGAREEGLGERE 59
Query: 87 SKFKTGGGGGRS 98
GGG GRS
Sbjct: 58 G----GGG*GRS 35
>TC11304 similar to PIR|T04669|T04669 serine O-acetyltransferase F8D20.150
- Arabidopsis thaliana {Arabidopsis
thaliana;} , partial (24%)
Length = 775
Score = 27.3 bits (59), Expect = 0.43
Identities = 21/59 (35%), Positives = 27/59 (45%), Gaps = 2/59 (3%)
Frame = -1
Query: 41 RSKGGQTRKEQLGTEGYQEMGRKGGLSTMEKSGGERAEE--EGIDIDESKFKTGGGGGR 97
R G+ R ++ G Q RK G + GG R E G + E +F GGGGGR
Sbjct: 574 RPISGRVRPSRVLVPGRQLPWRKQGRLSR---GGRRRRE*GGGGRVQERRFGGGGGGGR 407
>BP034485
Length = 485
Score = 27.3 bits (59), Expect = 0.43
Identities = 23/85 (27%), Positives = 39/85 (45%), Gaps = 1/85 (1%)
Frame = +3
Query: 14 KAKQGETVVPGGTGGKSLEAQEHLAEGRSKGGQTRKEQ-LGTEGYQEMGRKGGLSTMEKS 72
K+K+G+ GG GG S ++ + ++ GG K LG + + G GG + +
Sbjct: 15 KSKEGKQ--KGGGGGDSSSDKKKKTKDKNGGGFLVKFLGLGKKSKKGGGGGGGETANKNK 188
Query: 73 GGERAEEEGIDIDESKFKTGGGGGR 97
+ G + + K GGGGG+
Sbjct: 189NKNKNNGGGNNKGKEGKKGGGGGGK 263
>CB828183
Length = 555
Score = 26.9 bits (58), Expect = 0.56
Identities = 14/51 (27%), Positives = 24/51 (46%)
Frame = +2
Query: 3 SKQQNRQELEEKAKQGETVVPGGTGGKSLEAQEHLAEGRSKGGQTRKEQLG 53
S Q++ + + + VVP G KS E + +G + QTR+ +G
Sbjct: 332 SAVQSQGSISSASVTNQAVVPHGVSSKSSNPTEFVNQGLNLWNQTRQRWVG 484
>TC9175 weakly similar to UP|INAD_DROME (Q24008)
Inactivation-no-after-potential D protein, partial (5%)
Length = 692
Score = 26.6 bits (57), Expect = 0.73
Identities = 21/76 (27%), Positives = 30/76 (38%)
Frame = +2
Query: 6 QNRQELEEKAKQGETVVPGGTGGKSLEAQEHLAEGRSKGGQTRKEQLGTEGYQEMGRKGG 65
Q E E+K + E P GK E +E +E+ E +E G +
Sbjct: 56 QPEPEPEKKEEGKEEEKPAAEEGKPEEKKE-------------EEKAAEEAKKEEGGEEK 196
Query: 66 LSTMEKSGGERAEEEG 81
E+ GGE +EEG
Sbjct: 197 NDNKEEKGGEEGKEEG 244
>BP030058
Length = 455
Score = 26.6 bits (57), Expect = 0.73
Identities = 18/56 (32%), Positives = 22/56 (39%)
Frame = +1
Query: 44 GGQTRKEQLGTEGYQEMGRKGGLSTMEKSGGERAEEEGIDIDESKFKTGGGGGRSQ 99
GG + G EGY G +GG GG + G + GGGGG Q
Sbjct: 253 GGDKGLQFCGQEGYFGGGGEGGGGGGGDGGGGGGDGHGSGGGGGEGDGGGGGGSPQ 420
>TC12771 similar to UP|Q945N7 (Q945N7) AT5g04420/T32M21_20, partial (35%)
Length = 881
Score = 26.6 bits (57), Expect = 0.73
Identities = 13/23 (56%), Positives = 14/23 (60%)
Frame = +2
Query: 76 RAEEEGIDIDESKFKTGGGGGRS 98
RA GI IDES F GGG +S
Sbjct: 263 RAGHAGITIDESWFIVGGGDNKS 331
>TC15082 similar to GB|AAM16172.1|20334832|AY094016 At2g39720/T5I7.2
{Arabidopsis thaliana;}, partial (5%)
Length = 534
Score = 25.8 bits (55), Expect = 1.2
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = +2
Query: 72 SGGERAEEEGIDIDESKFKTGGGGGRSQ 99
SGG RA E + ++ GGGGG S+
Sbjct: 5 SGGRRAGESHFPVVYTEVDGGGGGGSSR 88
>TC9308 weakly similar to UP|Q9SQ55 (Q9SQ55) Nuclear RNA binding protein A,
partial (9%)
Length = 562
Score = 25.4 bits (54), Expect = 1.6
Identities = 16/60 (26%), Positives = 24/60 (39%)
Frame = +2
Query: 40 GRSKGGQTRKEQLGTEGYQEMGRKGGLSTMEKSGGERAEEEGIDIDESKFKTGGGGGRSQ 99
G+ +GG+ R E EG G +GG ++ G R + G G GR +
Sbjct: 311 GKGRGGRARGEGAPAEGAPAEGARGGRGRGGRARGPRG------------RRGAGRGRGE 454
>TC17395 similar to UP|Q9LN83 (Q9LN83) T12C24.17, partial (37%)
Length = 570
Score = 25.4 bits (54), Expect = 1.6
Identities = 25/92 (27%), Positives = 37/92 (40%), Gaps = 7/92 (7%)
Frame = -3
Query: 10 ELEEKAKQGETVVPGGTGGKSLEAQEHLAEGRSKGGQTRKE--QLGTEGYQE--MGRKGG 65
E+ E ++ + G +EH E GG+ E + T+G E +G G
Sbjct: 337 EVAETEEERDDAASGAVKEHGSVTEEHGDEEVEIGGEAEGEVGEENTDGGGEDVLGEATG 158
Query: 66 LST---MEKSGGERAEEEGIDIDESKFKTGGG 94
E++GG+ AEEE D D G G
Sbjct: 157 DEAPEEAERNGGDEAEEEAEDGDGDADGCGHG 62
>TC14308 homologue to UP|H2B_GOSHI (O22582) Histone H2B, partial (96%)
Length = 764
Score = 25.4 bits (54), Expect = 1.6
Identities = 21/61 (34%), Positives = 31/61 (50%), Gaps = 4/61 (6%)
Frame = +2
Query: 6 QNRQELE-EKAKQGETVV---PGGTGGKSLEAQEHLAEGRSKGGQTRKEQLGTEGYQEMG 61
Q RQE E+A++GE V G GG+ + +E S+GG++R + E QE
Sbjct: 83 QGRQEARGEEARRGEEVDGG*EGSPGGEEAQGREEA----SQGGRSRCRRQEEEEKQEER 250
Query: 62 R 62
R
Sbjct: 251 R 253
>TC12507
Length = 914
Score = 25.0 bits (53), Expect = 2.1
Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 5/38 (13%)
Frame = -3
Query: 30 SLEAQEHL-----AEGRSKGGQTRKEQLGTEGYQEMGR 62
+L +EHL E RSK G+ R +L TEGY E +
Sbjct: 321 TLTTREHLDRESEIERRSKLGRGRMWRLLTEGYGEFAK 208
>TC12341 similar to GB|AAP37848.1|30725652|BT008489 At2g29650 {Arabidopsis
thaliana;}, partial (14%)
Length = 478
Score = 24.6 bits (52), Expect = 2.8
Identities = 12/31 (38%), Positives = 15/31 (47%)
Frame = -3
Query: 66 LSTMEKSGGERAEEEGIDIDESKFKTGGGGG 96
LST S G +E + + TGGGGG
Sbjct: 185 LSTTAPSAGNTEDEAAPQV*REQ*STGGGGG 93
>AV419982
Length = 415
Score = 24.6 bits (52), Expect = 2.8
Identities = 9/22 (40%), Positives = 14/22 (62%)
Frame = -3
Query: 78 EEEGIDIDESKFKTGGGGGRSQ 99
E+EG+D++ GGGGG +
Sbjct: 356 EDEGLDVEAEGGGGGGGGGERE 291
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.299 0.126 0.331
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 983,285
Number of Sequences: 28460
Number of extensions: 10541
Number of successful extensions: 82
Number of sequences better than 10.0: 63
Number of HSP's better than 10.0 without gapping: 79
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 82
length of query: 101
length of database: 4,897,600
effective HSP length: 77
effective length of query: 24
effective length of database: 2,706,180
effective search space: 64948320
effective search space used: 64948320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 17 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 44 (22.0 bits)
S2: 47 (22.7 bits)
Medicago: description of AC140721.4