BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140721.11 + phase: 0
(607 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC7922 UP|Q84V95 (Q84V95) Pyruvate decarboxylase 1 , partial (81%) 833 0.0
TC8485 UP|Q84V95 (Q84V95) Pyruvate decarboxylase 1 , partial (20%) 239 9e-64
AW719918 215 1e-56
BP059119 168 2e-42
AV427800 132 2e-31
TC16607 similar to UP|DCP1_PEA (P51850) Pyruvate decarboxylase i... 121 3e-28
BP074862 94 6e-20
TC12845 similar to UP|ILV1_BRANA (P27818) Acetolactate synthase ... 67 1e-11
TC17596 similar to UP|Q42768 (Q42768) Acetohydroxyacid synthase ... 54 6e-08
TC11402 similar to UP|Q9LJZ9 (Q9LJZ9) Protein arginine N-methylt... 30 0.86
BP058840 29 2.5
AV768941 27 7.3
AV417442 27 7.3
AV417531 27 9.5
TC18823 similar to UP|Q9AYT7 (Q9AYT7) IDI2, partial (57%) 27 9.5
>TC7922 UP|Q84V95 (Q84V95) Pyruvate decarboxylase 1 , partial (81%)
Length = 1547
Score = 833 bits (2152), Expect = 0.0
Identities = 407/491 (82%), Positives = 443/491 (89%)
Frame = +3
Query: 1 MDTNLGSLEACKSPCNDIITTPTSNGTVSTIQKSPSTQSLASSESTLGSHLARRLVEVGI 60
MD LGSL+ K ND+++ T+N T + PST ++++ ++TLG HLARRL ++G+
Sbjct: 78 MDVKLGSLDTTKPASNDVVSCATANHTTAIQSSIPST-AVSTCDATLGRHLARRLAQIGV 254
Query: 61 TDIFTVPGDFNLTLLDHLIAEPKLKNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGL 120
TD+F+VPGDFNLTLLDHLIAEP+L +GCCNELNAGYAADGYAR+RGVGACVVTFTVGGL
Sbjct: 255 TDVFSVPGDFNLTLLDHLIAEPQLNLVGCCNELNAGYAADGYARARGVGACVVTFTVGGL 434
Query: 121 SVINAIAGAYSENLPVICIVGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVV 180
SV+NAIAGAYSENLP+ICIVGGPNSND+GT+RILHHTIGLPDF+QELRCFQTVTC+QAVV
Sbjct: 435 SVLNAIAGAYSENLPLICIVGGPNSNDYGTSRILHHTIGLPDFTQELRCFQTVTCFQAVV 614
Query: 181 NNLEDAHEMIDTAISTALKESKPVYISISCNLASIPHPTFSREPVPFSLSPKLSNPMGLE 240
NNLEDAHE IDTAISTALKESKPVYISISCNL IPHPTFSR+PVPFSLS KLSN MGLE
Sbjct: 615 NNLEDAHEQIDTAISTALKESKPVYISISCNLPGIPHPTFSRDPVPFSLSAKLSNQMGLE 794
Query: 241 AAVEAAAEVLNKAVKPVLVAGPKLRVAKACEAFIELADKSAYPYSVMPSAKGLIPEDHKH 300
AAVEA AE LNKAVKPVLV GPKLRVAKA +AF+ELAD S Y +VMPSAKG +PE H H
Sbjct: 795 AAVEATAEFLNKAVKPVLVGGPKLRVAKASDAFVELADASGYALAVMPSAKGQVPEHHPH 974
Query: 301 FIGTFWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVVIGN 360
FIGT+WGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVVI N
Sbjct: 975 FIGTYWGAVSTAFCAEIVESADAYLFAGPIFNDYSSVGYSLLLKKEKAIIVQPDRVVIAN 1154
Query: 361 GPTFGCVLMKDFLSALAKRLKRNNTAYENYFRIFVPEGLPVKSEPREPLRVNVLFQHIQN 420
GP FGCVLMKDFL ALAKRLK NN AYENY RIFVPEG P+KS P+EPLRVNV+FQHIQ
Sbjct: 1155GPAFGCVLMKDFLKALAKRLKHNNAAYENYHRIFVPEGKPLKSAPKEPLRVNVMFQHIQK 1334
Query: 421 MLSSETAVIAETGDSWFNCQKLKLPKGCGYEFQMQYGSIGWSVGATLGYAQAVPEKRVIA 480
MLS ETAVIAETGDSWFNCQKLKLP+GCGYEFQMQYGSIGWSVGATLGYAQAVPEKRVIA
Sbjct: 1335MLSGETAVIAETGDSWFNCQKLKLPRGCGYEFQMQYGSIGWSVGATLGYAQAVPEKRVIA 1514
Query: 481 CIGDGSFQVTA 491
CIGDGSFQVTA
Sbjct: 1515CIGDGSFQVTA 1547
>TC8485 UP|Q84V95 (Q84V95) Pyruvate decarboxylase 1 , partial (20%)
Length = 703
Score = 239 bits (610), Expect = 9e-64
Identities = 110/119 (92%), Positives = 115/119 (96%)
Frame = +1
Query: 489 VTAQDVSTMLRCGQKTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLVDAIHNGQGHCW 548
VTAQDVSTMLRCGQ TIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGL++AIHNG+G CW
Sbjct: 1 VTAQDVSTMLRCGQNTIIFLINNGGYTIEVEIHDGPYNVIKNWNYTGLIEAIHNGEGKCW 180
Query: 549 TTKVFCEEELVEAIETATGPKKDCLCFIEVIVHKDDTSKELLEWGSRVSSANSRPPNPQ 607
T+KVFCEEELVEAI TATGPKKDCLCFIEVIVHKDDTSKELLEWGSRVS+AN RPPNPQ
Sbjct: 181 TSKVFCEEELVEAIATATGPKKDCLCFIEVIVHKDDTSKELLEWGSRVSAANGRPPNPQ 357
>AW719918
Length = 506
Score = 215 bits (548), Expect = 1e-56
Identities = 105/137 (76%), Positives = 118/137 (85%)
Frame = +3
Query: 22 PTSNGTVSTIQKSPSTQSLASSESTLGSHLARRLVEVGITDIFTVPGDFNLTLLDHLIAE 81
P S+ +T SP S + + TLG HLARRLVE+G+ D+F+VPGDFNLTLLDHLI E
Sbjct: 102 PQSSMETATQLTSPPPPS--AFDGTLGHHLARRLVEIGVRDVFSVPGDFNLTLLDHLIGE 275
Query: 82 PKLKNIGCCNELNAGYAADGYARSRGVGACVVTFTVGGLSVINAIAGAYSENLPVICIVG 141
P+L IGCCNELNAGYAADGYAR++GVGACVVTFTVGGLSV+NAIAGAYSENLPVICIVG
Sbjct: 276 PQLNVIGCCNELNAGYAADGYARAKGVGACVVTFTVGGLSVLNAIAGAYSENLPVICIVG 455
Query: 142 GPNSNDFGTNRILHHTI 158
GPNSND+GTNRILHHTI
Sbjct: 456 GPNSNDYGTNRILHHTI 506
>BP059119
Length = 390
Score = 168 bits (426), Expect = 2e-42
Identities = 77/108 (71%), Positives = 92/108 (84%)
Frame = -1
Query: 274 IELADKSAYPYSVMPSAKGLIPEDHKHFIGTFWGAVSTAFCAEIVESADAYLFAGPIFND 333
+E A+ S Y +VMPS KG +PEDH HFIGT+WGAVST +C EIVESADAY+F GPIFND
Sbjct: 390 LEFAEASGYAIAVMPSGKGFVPEDHPHFIGTYWGAVSTGYCGEIVESADAYIFVGPIFND 211
Query: 334 YSSVGYSLLLKKEKAIIVQPDRVVIGNGPTFGCVLMKDFLSALAKRLK 381
YSSVGYSLL+KKEKAI+VQP+RV IGNGP+ G V M DFL+AL+K++K
Sbjct: 210 YSSVGYSLLVKKEKAIMVQPNRVTIGNGPSLGWVFMADFLTALSKKVK 67
>AV427800
Length = 246
Score = 132 bits (331), Expect = 2e-31
Identities = 65/81 (80%), Positives = 72/81 (88%)
Frame = +3
Query: 184 EDAHEMIDTAISTALKESKPVYISISCNLASIPHPTFSREPVPFSLSPKLSNPMGLEAAV 243
EDAHE++DTAISTALKESKPVYISISCNL +I HPTF+R+PVPF LSPK+SN GLEAAV
Sbjct: 3 EDAHELLDTAISTALKESKPVYISISCNLPAIHHPTFARDPVPFFLSPKVSNQEGLEAAV 182
Query: 244 EAAAEVLNKAVKPVLVAGPKL 264
E AA LNKAVKPV+V GPKL
Sbjct: 183 EVAAAFLNKAVKPVIVGGPKL 245
>TC16607 similar to UP|DCP1_PEA (P51850) Pyruvate decarboxylase isozyme 1
(PDC) , partial (13%)
Length = 517
Score = 121 bits (304), Expect = 3e-28
Identities = 55/76 (72%), Positives = 64/76 (83%)
Frame = +1
Query: 532 NYTGLVDAIHNGQGHCWTTKVFCEEELVEAIETATGPKKDCLCFIEVIVHKDDTSKELLE 591
+YT V+AI+NGQG CWT KV E++L EAI ATGP+KD LCFIEV VHKDDTS+ELLE
Sbjct: 1 DYTRFVEAINNGQGKCWTAKVRTEDDLTEAIAAATGPQKDSLCFIEVFVHKDDTSQELLE 180
Query: 592 WGSRVSSANSRPPNPQ 607
WGSRV++AN RPPNPQ
Sbjct: 181 WGSRVAAANGRPPNPQ 228
>BP074862
Length = 422
Score = 94.0 bits (232), Expect = 6e-20
Identities = 46/73 (63%), Positives = 59/73 (80%), Gaps = 2/73 (2%)
Frame = -3
Query: 537 VDAIHNGQGHCWTTKVFCEEELVEAIETATGPK-KDCLCFIEVIVHKD-DTSKELLEWGS 594
++AI +G+G CWT+KVFC EE+VEAI T P+ DCLCFIEVIVH+ ++ELLEWGS
Sbjct: 420 IEAIPHGEGKCWTSKVFC*EEVVEAIAHCTRPQATDCLCFIEVIVHQG*YHAQELLEWGS 241
Query: 595 RVSSANSRPPNPQ 607
RVS+A+ RPP+PQ
Sbjct: 240 RVSAAHGRPPHPQ 202
>TC12845 similar to UP|ILV1_BRANA (P27818) Acetolactate synthase I,
chloroplast precursor (Acetohydroxy-acid synthase I)
(ALS I) , partial (35%)
Length = 910
Score = 66.6 bits (161), Expect = 1e-11
Identities = 59/246 (23%), Positives = 111/246 (44%), Gaps = 5/246 (2%)
Frame = +2
Query: 21 TPTSNGTVSTIQKSPSTQSLASSESTLGSH-LARRLVEVGITDIFTVPGDFNLTLLDHLI 79
TPT + + T + E G+ L L G+TD+F PG ++ + L
Sbjct: 173 TPTGASASTGVATENFTSRFSLDEPRKGADILVEALERQGVTDVFAYPGGASMEIHQALT 352
Query: 80 AEPKLKNIGCCNELNAGYAADGYARSRGV-GACVVTFTVGGLSVINAIAGAYSENLPVIC 138
++N+ +E +AA+GYARS G+ G C+ T G ++++ +A A +++P+I
Sbjct: 353 RSDTIRNVLPRHEQGGIFAAEGYARSSGLPGVCIATSGPGATNLVSGLADALLDSVPLIA 532
Query: 139 IVGGPNSNDFGTNRILHHTIGLPDFSQELRCFQTVTCYQAVVNNLEDAHEMIDTAISTAL 198
I G GT+ I + +++T + +V +++D +++ A A
Sbjct: 533 ITGQVPRRMIGTDAFQETPI--------VEVTRSITKHNYLVLDIDDIPRIVNEAFFLAT 688
Query: 199 K-ESKPVYISISCNL-ASIPHPTFSRE-PVPFSLSPKLSNPMGLEAAVEAAAEVLNKAVK 255
PV I I ++ + P + + +P ++ +P +A +E +L + K
Sbjct: 689 SGRPGPVLIDIPKDIQQQLAVPNWDQPIKLPGYMARLPRSPS--DALLEQVVRLLLECKK 862
Query: 256 PVLVAG 261
PVL AG
Sbjct: 863 PVLYAG 880
>TC17596 similar to UP|Q42768 (Q42768) Acetohydroxyacid synthase , partial
(16%)
Length = 537
Score = 54.3 bits (129), Expect = 6e-08
Identities = 34/116 (29%), Positives = 58/116 (49%), Gaps = 2/116 (1%)
Frame = +3
Query: 22 PTSNGTVSTIQKSPSTQSLASSESTLGSH-LARRLVEVGITDIFTVPGDFNLTLLDHLIA 80
PT+ V T T + E G+ L L G+T++F PG +L + L
Sbjct: 204 PTAAPAVETF-----TSRFPADEPRKGADILVEALEREGVTNVFAYPGGASLEIHQALTR 368
Query: 81 EPKLKNIGCCNELNAGYAADGYARSRGV-GACVVTFTVGGLSVINAIAGAYSENLP 135
P ++N+ +E +AA+GYARS G+ G C+ T G ++++ +A A +++P
Sbjct: 369 SPTIRNVLPRHEQGGVFAAEGYARSSGLPGVCIATSGPGATNLVSGLADAMLDSIP 536
>TC11402 similar to UP|Q9LJZ9 (Q9LJZ9) Protein arginine
N-methyltransferase-like protein, partial (28%)
Length = 536
Score = 30.4 bits (67), Expect = 0.86
Identities = 21/80 (26%), Positives = 37/80 (46%), Gaps = 4/80 (5%)
Frame = -3
Query: 153 ILHHTIGLPDFSQELRCFQTVTCYQAVVNNLEDAHEMIDTAISTALKE----SKPVYISI 208
IL+ ++ D +++ CF C Q V + H + T +ST ++ + + +
Sbjct: 519 ILNSSMQYNDNIRQVICFNNFICLQGNVTRINCIHP-LRTGLSTKYRKDSCTASNINYNF 343
Query: 209 SCNLASIPHPTFSREPVPFS 228
+CN +PH F PV FS
Sbjct: 342 ACNKTLMPHDCF---PVSFS 292
>BP058840
Length = 464
Score = 28.9 bits (63), Expect = 2.5
Identities = 16/38 (42%), Positives = 23/38 (60%)
Frame = +2
Query: 22 PTSNGTVSTIQKSPSTQSLASSESTLGSHLARRLVEVG 59
P +N STI S ++ SLA + STL L R+L+ +G
Sbjct: 119 PHTNPKRSTISLSLTSSSLALTSSTLSIQLRRKLLPMG 232
>AV768941
Length = 242
Score = 27.3 bits (59), Expect = 7.3
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = -2
Query: 592 WGSRVSSANSRPPNPQ 607
WGSRV A PPNPQ
Sbjct: 238 WGSRVFFAKGCPPNPQ 191
>AV417442
Length = 391
Score = 27.3 bits (59), Expect = 7.3
Identities = 12/39 (30%), Positives = 20/39 (50%)
Frame = +3
Query: 4 NLGSLEACKSPCNDIITTPTSNGTVSTIQKSPSTQSLAS 42
N+ ++ +PC +T + NGT T + S +SL S
Sbjct: 177 NVSAISTSFTPCMSFLTNSSGNGTSPTAECCDSIKSLTS 293
>AV417531
Length = 300
Score = 26.9 bits (58), Expect = 9.5
Identities = 13/44 (29%), Positives = 20/44 (44%)
Frame = -2
Query: 179 VVNNLEDAHEMIDTAISTALKESKPVYISISCNLASIPHPTFSR 222
V++ E DT + A+ P Y+ +C L PH FS+
Sbjct: 167 VLSKTNGPEEKSDTLFAIAVAFISP*YLLHTCQLKDFPHTHFSK 36
>TC18823 similar to UP|Q9AYT7 (Q9AYT7) IDI2, partial (57%)
Length = 785
Score = 26.9 bits (58), Expect = 9.5
Identities = 13/41 (31%), Positives = 19/41 (45%)
Frame = +1
Query: 68 GDFNLTLLDHLIAEPKLKNIGCCNELNAGYAADGYARSRGV 108
G + T + H + +PKL + CN A GY + GV
Sbjct: 547 GSYGATFIQHQVEKPKLSVLTHCN--TGSLATAGYGTALGV 663
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.407
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,842,486
Number of Sequences: 28460
Number of extensions: 148458
Number of successful extensions: 768
Number of sequences better than 10.0: 30
Number of HSP's better than 10.0 without gapping: 759
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 765
length of query: 607
length of database: 4,897,600
effective HSP length: 96
effective length of query: 511
effective length of database: 2,165,440
effective search space: 1106539840
effective search space used: 1106539840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 58 (26.9 bits)
Medicago: description of AC140721.11