BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140721.1 - phase: 0
(313 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15921 weakly similar to GB|AAB64701.1|603415|SCE9163 Yer174cp ... 249 3e-67
TC19556 238 7e-64
BP038900 99 7e-22
TC11207 weakly similar to GB|AAB64701.1|603415|SCE9163 Yer174cp ... 90 6e-19
AV418998 64 4e-11
AV777936 59 1e-09
TC12498 similar to GB|AAP21204.1|30102572|BT006396 At3g15660 {Ar... 47 1e-09
AI967451 44 5e-05
TC10225 similar to UP|Q8LFQ6 (Q8LFQ6) Glutaredoxin, partial (69%) 35 0.021
TC9884 weakly similar to GB|AAO63337.1|28950827|BT005273 At5g585... 33 0.062
TC14293 similar to UP|Q9FYE4 (Q9FYE4) EF-hand calcium binding pr... 32 0.11
TC18562 homologue to UP|Q9FVA6 (Q9FVA6) Glu-tRNA(Gln) amidotrans... 32 0.11
TC7933 weakly similar to UP|AAQ56728 (AAQ56728) Polygalacturonas... 32 0.14
TC14876 weakly similar to UP|Q9ZVB8 (Q9ZVB8) At2g41330 protein (... 31 0.24
AV409090 29 1.2
TC14946 similar to UP|Q9SXS8 (Q9SXS8) Ethylene responsive elemen... 29 1.2
TC12033 29 1.2
BI420843 29 1.2
AV408970 28 1.5
TC15366 similar to GB|AAO63329.1|28950811|BT005265 At5g21920 {Ar... 28 1.5
>TC15921 weakly similar to GB|AAB64701.1|603415|SCE9163 Yer174cp
{Saccharomyces cerevisiae;} , partial (10%)
Length = 580
Score = 249 bits (637), Expect = 3e-67
Identities = 119/135 (88%), Positives = 128/135 (94%)
Frame = +1
Query: 179 GNESGNATWVRPQPKKKADLRLTPGRHVQLTVPLEELVDKLVKENKVVAFIKGSRSAPLC 238
GNESG TWVR QPKKK DLRLTPGRHVQLTVPLE+L+DKLVK+NKVVAFIKGSRSAP+C
Sbjct: 1 GNESGTTTWVRQQPKKKPDLRLTPGRHVQLTVPLEDLIDKLVKDNKVVAFIKGSRSAPMC 180
Query: 239 GFSQKVIGILEKEGVDYESVDVLDEDYNYGLRETLKKYSNWPTFPQIFLNGELVGGCDIL 298
GFSQ+VIGILE EGVDYESV+VLDEDYNYGLRETLKKYSNWPTFPQIF++GELVGGCDIL
Sbjct: 181 GFSQRVIGILESEGVDYESVNVLDEDYNYGLRETLKKYSNWPTFPQIFVDGELVGGCDIL 360
Query: 299 TSMNEKGEVAGLFKK 313
TSM EKGEVA +FKK
Sbjct: 361 TSMYEKGEVASVFKK 405
>TC19556
Length = 495
Score = 238 bits (608), Expect = 7e-64
Identities = 117/164 (71%), Positives = 131/164 (79%)
Frame = +1
Query: 21 INLSSTQLLSYHHFPSFSPQNTPTLSLNFHPNSSTNLRPISLKPYHPRKPQSWLVAMAVK 80
INLSS Q LS+H S PQNT TLS N H S N RPISLKPY+ P+ W V++AVK
Sbjct: 7 INLSSMQALSFHRLSSSHPQNTRTLSFNLHSKPSFNPRPISLKPYNSDNPRKWTVSLAVK 186
Query: 81 NLADTSPVTVSPENDGLTGEELPSGAGVYAVYDKNGELQFIGLSRNIAATVLAHRKSVPE 140
L +T V VSPENDG GE P GAGVYAVYDK+GELQFIG+SRN+AA+V HRKSVPE
Sbjct: 187 TLGETEAVAVSPENDGPAGEFHP-GAGVYAVYDKDGELQFIGISRNVAASVSTHRKSVPE 363
Query: 141 LCGSFKVGVVDEPDRESLTQAWKSWMEEHIKITGKVPPGNESGN 184
LCGSFKVG+VDEPDRE+LTQAWKSWMEE+IK+ GKVPPGNESGN
Sbjct: 364 LCGSFKVGIVDEPDREALTQAWKSWMEEYIKVNGKVPPGNESGN 495
>BP038900
Length = 471
Score = 99.4 bits (246), Expect = 7e-22
Identities = 45/98 (45%), Positives = 68/98 (68%)
Frame = -1
Query: 216 VDKLVKENKVVAFIKGSRSAPLCGFSQKVIGILEKEGVDYESVDVLDEDYNYGLRETLKK 275
+DK+V NKVV F+KG++ P CGFS V+ IL+ V +E++++L+ D LR+ LK+
Sbjct: 468 LDKVVTSNKVVLFMKGTKDFPQCGFSNTVVQILKSLNVPFETLNILENDI---LRQGLKE 298
Query: 276 YSNWPTFPQIFLNGELVGGCDILTSMNEKGEVAGLFKK 313
Y+NWPTFPQ++++GE GGCDI + GE+ L +K
Sbjct: 297 YTNWPTFPQLYIDGEFFGGCDITVEAYKNGELQELLEK 184
>TC11207 weakly similar to GB|AAB64701.1|603415|SCE9163 Yer174cp
{Saccharomyces cerevisiae;} , partial (29%)
Length = 476
Score = 89.7 bits (221), Expect = 6e-19
Identities = 39/91 (42%), Positives = 62/91 (67%)
Frame = +2
Query: 217 DKLVKENKVVAFIKGSRSAPLCGFSQKVIGILEKEGVDYESVDVLDEDYNYGLRETLKKY 276
+++ + V+ F+KG+ AP CGFS +V+G L++EGV + D+L +D +R+ LK +
Sbjct: 8 EEIDASSPVMLFMKGTPDAPRCGFSSRVVGALQEEGVVFGHFDILTDDE---VRQGLKTF 178
Query: 277 SNWPTFPQIFLNGELVGGCDILTSMNEKGEV 307
SNWPTFPQ++ EL+GGCDI+ + GE+
Sbjct: 179 SNWPTFPQLYYKSELIGGCDIVMELRNNGEL 271
>AV418998
Length = 428
Score = 63.5 bits (153), Expect = 4e-11
Identities = 25/44 (56%), Positives = 36/44 (81%)
Frame = +3
Query: 269 LRETLKKYSNWPTFPQIFLNGELVGGCDILTSMNEKGEVAGLFK 312
+R+ +KK+SNWPTFPQ++ GEL+GGCDI +M+E GE+ +FK
Sbjct: 9 VRDGIKKFSNWPTFPQLYCKGELLGGCDIAIAMHESGELHEVFK 140
Score = 59.7 bits (143), Expect = 6e-10
Identities = 29/76 (38%), Positives = 46/76 (60%)
Frame = +3
Query: 204 RHVQLTVPLEELVDKLVKENKVVAFIKGSRSAPLCGFSQKVIGILEKEGVDYESVDVLDE 263
+ L+ L ++ LV + V+ F+KG P CGFS+KV+ IL++E V + S D+L +
Sbjct: 210 KSTDLSTTLTSRLESLVNSSPVMLFMKGKPDEPKCGFSRKVVEILQQESVPFNSFDILTD 389
Query: 264 DYNYGLRETLKKYSNW 279
+ +R+ LK YSNW
Sbjct: 390 E---EVRQGLKVYSNW 428
>AV777936
Length = 436
Score = 58.9 bits (141), Expect = 1e-09
Identities = 34/105 (32%), Positives = 58/105 (54%), Gaps = 1/105 (0%)
Frame = +3
Query: 184 NATWVRPQPKKKADLRLTPGRHVQLT-VPLEELVDKLVKENKVVAFIKGSRSAPLCGFSQ 242
N T V P D + P ++ T + L +V++ VK+N V+ ++KG P CGFS
Sbjct: 135 NFTKVSDDPDTHDDFK--PANKLEGTAISLSNVVEQDVKDNPVMIYMKGVPEFPQCGFSS 308
Query: 243 KVIGILEKEGVDYESVDVLDEDYNYGLRETLKKYSNWPTFPQIFL 287
+ +L+ V + ++L++ L+ +K +S+WPTFPQIF+
Sbjct: 309 LAVRVLKHYDVPLSARNILED---AELKNAVKAFSHWPTFPQIFI 434
>TC12498 similar to GB|AAP21204.1|30102572|BT006396 At3g15660 {Arabidopsis
thaliana;}, partial (70%)
Length = 605
Score = 47.4 bits (111), Expect(2) = 1e-09
Identities = 20/34 (58%), Positives = 24/34 (69%)
Frame = +3
Query: 274 KKYSNWPTFPQIFLNGELVGGCDILTSMNEKGEV 307
K SNWPTFP IF+ GE VGG DI+ M+ GE+
Sbjct: 321 KPSSNWPTFPPIFIKGEFVGGSDIILHMHPTGEL 422
Score = 31.2 bits (69), Expect(2) = 1e-09
Identities = 15/52 (28%), Positives = 29/52 (54%)
Frame = +2
Query: 212 LEELVDKLVKENKVVAFIKGSRSAPLCGFSQKVIGILEKEGVDYESVDVLDE 263
L +V++ VK+N V+ ++KG P GFS + +L V + ++L++
Sbjct: 143 LANIVEQDVKDNPVMIYMKGVPEYPPSGFSSLPVRVLNLYDVSISASNILED 298
>AI967451
Length = 425
Score = 43.5 bits (101), Expect = 5e-05
Identities = 25/106 (23%), Positives = 52/106 (48%)
Frame = +3
Query: 205 HVQLTVPLEELVDKLVKENKVVAFIKGSRSAPLCGFSQKVIGILEKEGVDYESVDVLDED 264
H ++ +P + ++V N VV F K C F V + GV+Y+ +++ E
Sbjct: 60 HSKMALPKAK---EIVSSNAVVVFSK-----TYCPFCVDVKKLFANLGVNYKVIELDSEA 215
Query: 265 YNYGLRETLKKYSNWPTFPQIFLNGELVGGCDILTSMNEKGEVAGL 310
++ L +++ + P +F+ G +GGCD +++ +G++ L
Sbjct: 216 DGSDIQAALAEWTGQRSVPNVFIGGNHIGGCDSTKALHNQGKLVPL 353
>TC10225 similar to UP|Q8LFQ6 (Q8LFQ6) Glutaredoxin, partial (69%)
Length = 725
Score = 34.7 bits (78), Expect = 0.021
Identities = 28/97 (28%), Positives = 45/97 (45%)
Frame = +3
Query: 214 ELVDKLVKENKVVAFIKGSRSAPLCGFSQKVIGILEKEGVDYESVDVLDEDYNYGLRETL 273
E V + +K+V F K P C ++ V L + V Y V++ + D +++ L
Sbjct: 144 EFVHNTISSHKIVIFSK--TYCPYCNRAKAVFKELNQ--VPYV-VELDERDDGSKIQDYL 308
Query: 274 KKYSNWPTFPQIFLNGELVGGCDILTSMNEKGEVAGL 310
T PQ+F+NG+ +GG D E G +A L
Sbjct: 309 INIVGKRTVPQVFINGKHLGGSDDTVEAYESGLLAKL 419
>TC9884 weakly similar to GB|AAO63337.1|28950827|BT005273 At5g58530
{Arabidopsis thaliana;}, partial (31%)
Length = 557
Score = 33.1 bits (74), Expect = 0.062
Identities = 13/32 (40%), Positives = 20/32 (61%)
Frame = +2
Query: 281 TFPQIFLNGELVGGCDILTSMNEKGEVAGLFK 312
T P++F+ G VGG + + MNE GE+ + K
Sbjct: 2 TLPRVFIGGRYVGGAEEVRQMNEVGELKKILK 97
>TC14293 similar to UP|Q9FYE4 (Q9FYE4) EF-hand calcium binding protein-like
(At5g04170), partial (18%)
Length = 540
Score = 32.3 bits (72), Expect = 0.11
Identities = 21/76 (27%), Positives = 36/76 (46%), Gaps = 1/76 (1%)
Frame = +3
Query: 14 HSSMATIINLSSTQLLSYHHFPSFSPQNTPTLSLNFHPN-SSTNLRPISLKPYHPRKPQS 72
H+S S L +H P+ P++ +L+ HPN ++ +LR +SL P P +
Sbjct: 153 HTSHTAPPRHPSPTALRHHLNPTAQPRHPNPTALHRHPNPTAVHLRLLSLTPLAPTASRP 332
Query: 73 WLVAMAVKNLADTSPV 88
L K+ T+P+
Sbjct: 333 LLTPRPTKSPPRTNPI 380
>TC18562 homologue to UP|Q9FVA6 (Q9FVA6) Glu-tRNA(Gln) amidotransferase
subunit A, partial (4%)
Length = 239
Score = 32.3 bits (72), Expect = 0.11
Identities = 22/53 (41%), Positives = 26/53 (48%), Gaps = 12/53 (22%)
Frame = +3
Query: 32 HHFPSFSPQNTPTLSLNFHPNSSTNLRPISLK------------PYHPRKPQS 72
H PSF TP+LSL P ST+L P+SLK P H R P+S
Sbjct: 78 HASPSFLQPQTPSLSL---PPPSTSLPPLSLKLPSPTTNPQPPPPLHRRNPRS 227
>TC7933 weakly similar to UP|AAQ56728 (AAQ56728) Polygalacturonase
inhibiting protein, partial (78%)
Length = 1320
Score = 32.0 bits (71), Expect = 0.14
Identities = 43/180 (23%), Positives = 73/180 (39%), Gaps = 13/180 (7%)
Frame = +3
Query: 17 MATIINLSSTQLLSYH---HFPSFSPQNTPTLSLNFHPNSSTNLRPISLKPYHPRKPQSW 73
+A + NL S L + H P+ + LSLN N T P S + P +
Sbjct: 426 LAQLKNLDSLYLSFNNLSGHIPASLSRLPNLLSLNLDRNKLTGPIPASFGFFKKPGPNLF 605
Query: 74 LVAMAVKNLADTSPVTVSPENDGLTGEELPSGAGVYAVYDKNGELQFIGLSRNIAATVLA 133
L + +S + PE + +L A + ++ N + Q + LSRN+ + L+
Sbjct: 606 LSHNQLSGPIPSSLGQLDPERIDFSRNKLTGNASL--LFAHNKKTQLLDLSRNLLSFDLS 779
Query: 134 ---HRKSVP-------ELCGSFKVGVVDEPDRESLTQAWKSWMEEHIKITGKVPPGNESG 183
KS+ ++ GS VG+ S Q + + + ++GK+P G E G
Sbjct: 780 KVEFPKSLTWLDLNHNKIHGSIPVGL-------SAVQNLQQFNVSYNLLSGKIPQGGELG 938
>TC14876 weakly similar to UP|Q9ZVB8 (Q9ZVB8) At2g41330 protein
(At2g41330/F13H10.12), partial (18%)
Length = 822
Score = 31.2 bits (69), Expect = 0.24
Identities = 11/30 (36%), Positives = 19/30 (62%)
Frame = +1
Query: 283 PQIFLNGELVGGCDILTSMNEKGEVAGLFK 312
PQ+F+ G VGG D++ + E GE+ + +
Sbjct: 55 PQVFIRGRCVGGADVIKQLCEVGELGKILE 144
>AV409090
Length = 436
Score = 28.9 bits (63), Expect = 1.2
Identities = 19/70 (27%), Positives = 32/70 (45%), Gaps = 2/70 (2%)
Frame = +1
Query: 6 LLLTHSTDHSSMATIINLSSTQLLSYHHFPSFSPQ--NTPTLSLNFHPNSSTNLRPISLK 63
+++TH+T H + ++L + + +H P + +PT S HP SS N P +
Sbjct: 145 IVITHTTPHMATFATVSLHANPISPSNHLPLNTTHLLQSPTSSTP-HPPSSLN--PTTSP 315
Query: 64 PYHPRKPQSW 73
P P W
Sbjct: 316 TSPPSSPTPW 345
>TC14946 similar to UP|Q9SXS8 (Q9SXS8) Ethylene responsive element binding
factor, partial (26%)
Length = 631
Score = 28.9 bits (63), Expect = 1.2
Identities = 11/33 (33%), Positives = 20/33 (60%)
Frame = +2
Query: 159 TQAWKSWMEEHIKITGKVPPGNESGNATWVRPQ 191
++A+ W E+H ++ + PP SG+A RP+
Sbjct: 62 SEAYLQWHEQHRRVLQRSPPCQTSGSAGCSRPE 160
>TC12033
Length = 1001
Score = 28.9 bits (63), Expect = 1.2
Identities = 19/65 (29%), Positives = 28/65 (42%), Gaps = 2/65 (3%)
Frame = +2
Query: 120 FIGLSRNIAATVLAHRKSVPELCGSFKVGVVDEPDRESLTQAWKSWMEEHIKITGKV--P 177
F GLSR T + PE+ + EP SL+ +++E + TG V P
Sbjct: 584 FYGLSRQFVPTAYVKKTQFPEISPQEFTVCISEPISPSLSIPGTGFIKETVAATGVVRSP 763
Query: 178 PGNES 182
P +S
Sbjct: 764 PEQKS 778
>BI420843
Length = 377
Score = 28.9 bits (63), Expect = 1.2
Identities = 13/34 (38%), Positives = 20/34 (58%)
Frame = +3
Query: 273 LKKYSNWPTFPQIFLNGELVGGCDILTSMNEKGE 306
L++ + T PQIF N +L+GG L ++ GE
Sbjct: 63 LRERTGSATVPQIFFNEKLIGGLVELNTLRNNGE 164
>AV408970
Length = 195
Score = 28.5 bits (62), Expect = 1.5
Identities = 16/46 (34%), Positives = 20/46 (42%), Gaps = 3/46 (6%)
Frame = +3
Query: 29 LSYHHFPSFSPQNTPTLSLNFHPNSSTNLRPISLKPYH---PRKPQ 71
L HH P P L+ HP L P+ L P H P++PQ
Sbjct: 57 LPLHHLSLSPPPPPPRRRLHPHPPPLPPLHPLPLHPRHHLLPQRPQ 194
>TC15366 similar to GB|AAO63329.1|28950811|BT005265 At5g21920 {Arabidopsis
thaliana;}, partial (23%)
Length = 568
Score = 28.5 bits (62), Expect = 1.5
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +3
Query: 27 QLLSYHHFPSFSPQNTPTLSLNFHPNSSTNLRP 59
++L +H FS +PT L+F P +STN P
Sbjct: 153 EILKHHSHSPFSNPQSPTSPLSFLPLNSTNPSP 251
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.315 0.134 0.397
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,039,447
Number of Sequences: 28460
Number of extensions: 93133
Number of successful extensions: 544
Number of sequences better than 10.0: 75
Number of HSP's better than 10.0 without gapping: 536
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 540
length of query: 313
length of database: 4,897,600
effective HSP length: 90
effective length of query: 223
effective length of database: 2,336,200
effective search space: 520972600
effective search space used: 520972600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 55 (25.8 bits)
Medicago: description of AC140721.1