BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140546.3 + phase: 0
(266 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16527 weakly similar to UP|IF3_SYNY3 (P72874) Translation init... 145 9e-36
TC16978 UP|R11A_LOTJA (Q40191) Ras-related protein Rab11A, complete 33 0.065
TC14123 similar to UP|Q93X25 (Q93X25) 2-Cys peroxiredoxin, parti... 29 0.72
TC8917 similar to UP|AAH64262 (AAH64262) MGC76273 protein, parti... 29 0.72
TC13164 similar to UP|Q8H5W9 (Q8H5W9) OJ1123_B01.7 protein, part... 27 2.7
AU089311 27 4.7
TC13064 similar to UP|Q9LUC0 (Q9LUC0) Gb|AAD10662.1, partial (20%) 26 6.1
TC17515 similar to UP|Q8WP00 (Q8WP00) Protamine P1, partial (36%) 26 6.1
TC19422 weakly similar to UP|Q945L7 (Q945L7) AT4g35240/F23E12_20... 26 6.1
TC16761 similar to GB|AAL32703.1|17065098|AY062625 nucleotide su... 26 6.1
TC18869 similar to UP|Q9SIR1 (Q9SIR1) At2g25320 protein, partial... 26 8.0
>TC16527 weakly similar to UP|IF3_SYNY3 (P72874) Translation initiation
factor IF-3, partial (24%)
Length = 1105
Score = 145 bits (365), Expect = 9e-36
Identities = 73/99 (73%), Positives = 83/99 (83%), Gaps = 1/99 (1%)
Frame = +3
Query: 157 MDLKELKMGYNIDQHDYSVRLKAARKFLSDGDKVKVIVNLKGREKEFRNNAIELIRRFQN 216
MDLKELKMGYNIDQHDYSVRL+AARKFL+DGDKVKVIVNLKGRE EFRN AIELI+RF
Sbjct: 3 MDLKELKMGYNIDQHDYSVRLRAARKFLTDGDKVKVIVNLKGRENEFRNIAIELIKRFPT 182
Query: 217 DVGKLATEEAKNFRDKNIFITLIPN-KTEVQKPQETPTK 254
DVG+L TEEA++FRD+NIFI L+P + + TP K
Sbjct: 183 DVGELGTEEAESFRDRNIFILLVPE*NCYTENSRTTPEK 299
>TC16978 UP|R11A_LOTJA (Q40191) Ras-related protein Rab11A, complete
Length = 984
Score = 32.7 bits (73), Expect = 0.065
Identities = 15/35 (42%), Positives = 22/35 (62%)
Frame = +2
Query: 224 EEAKNFRDKNIFITLIPNKTEVQKPQETPTKAATD 258
EE +N DKNI I LI NK ++ ++ PT+ A +
Sbjct: 398 EELRNHADKNIVIILIGNKCDLVNQRDVPTEDAKE 502
>TC14123 similar to UP|Q93X25 (Q93X25) 2-Cys peroxiredoxin, partial (89%)
Length = 1130
Score = 29.3 bits (64), Expect = 0.72
Identities = 18/47 (38%), Positives = 28/47 (59%), Gaps = 4/47 (8%)
Frame = +2
Query: 5 TSTTVVPVKLKASHFFSNK----LSIPHSLNLQSSPSFTLPSHATVR 47
++ ++ +KL + FS K LSIP+SLN S+ F+LPS + R
Sbjct: 68 SAASLFSLKLNPTPLFSPKPTTSLSIPNSLNPLSTKPFSLPSVSLTR 208
>TC8917 similar to UP|AAH64262 (AAH64262) MGC76273 protein, partial (3%)
Length = 676
Score = 29.3 bits (64), Expect = 0.72
Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 6/64 (9%)
Frame = +2
Query: 208 IELIRRFQNDVGKLATEEAKNFRDKNIFITLIPNKTEVQKPQE------TPTKAATDEVS 261
+E ++ + +V K TEE N +K++ L N+ E +KP+E KA T+EV
Sbjct: 203 VEQVQESEANVEK-KTEETPNEDNKSLSTLLNENEEEAKKPEEEVQAEPKEEKATTEEVK 379
Query: 262 VSVE 265
V +
Sbjct: 380 VETK 391
>TC13164 similar to UP|Q8H5W9 (Q8H5W9) OJ1123_B01.7 protein, partial (21%)
Length = 496
Score = 27.3 bits (59), Expect = 2.7
Identities = 17/41 (41%), Positives = 24/41 (58%), Gaps = 1/41 (2%)
Frame = +1
Query: 2 AGITSTTVVPVKLKAS-HFFSNKLSIPHSLNLQSSPSFTLP 41
+ +T T +P+ + H S SIP SL LQS+PS+ LP
Sbjct: 199 SNLTRTLSLPISAHSI*HILS---SIPSSLPLQSNPSWPLP 312
>AU089311
Length = 344
Score = 26.6 bits (57), Expect = 4.7
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 226 AKNFRDKNIFITLIPNKTEVQKPQETPTKAATD 258
A+ DKNI I LI NK ++ + PT+ A +
Sbjct: 2 ARGHADKNIVIMLIGNKCDLGSLRAVPTEDAEE 100
>TC13064 similar to UP|Q9LUC0 (Q9LUC0) Gb|AAD10662.1, partial (20%)
Length = 423
Score = 26.2 bits (56), Expect = 6.1
Identities = 21/68 (30%), Positives = 29/68 (41%), Gaps = 2/68 (2%)
Frame = +1
Query: 2 AGITSTTVVPVKLKASHFFSNKLSIPHSLNLQSSPSFTLPSHATVRYGVNLRPSAYGG-- 59
A TS +P + + + S S + SSPS + S R V +RP +
Sbjct: 19 ASSTSRRFIPAGPRPNVRGKRRRSYSPSSSSSSSPSPSSVSRRIARVAVRVRPPSPPAPP 198
Query: 60 GGNFRRAP 67
GG RRAP
Sbjct: 199GGRHRRAP 222
>TC17515 similar to UP|Q8WP00 (Q8WP00) Protamine P1, partial (36%)
Length = 546
Score = 26.2 bits (56), Expect = 6.1
Identities = 12/25 (48%), Positives = 16/25 (64%)
Frame = -1
Query: 21 SNKLSIPHSLNLQSSPSFTLPSHAT 45
SNKLS P S L++ PSF++ T
Sbjct: 180 SNKLSNPSSSALENKPSFSIEESVT 106
>TC19422 weakly similar to UP|Q945L7 (Q945L7) AT4g35240/F23E12_200, partial
(39%)
Length = 624
Score = 26.2 bits (56), Expect = 6.1
Identities = 24/87 (27%), Positives = 36/87 (40%)
Frame = +1
Query: 26 IPHSLNLQSSPSFTLPSHATVRYGVNLRPSAYGGGGNFRRAPPEKDADDGQALDLSTLSS 85
+PH L S PS + SH PS+ G APP+ D D G + LSS
Sbjct: 31 LPHCPWLSSQPSTSKTSH----------PSSTLDGV----APPDMDPDVGLSTTSRHLSS 168
Query: 86 NTVRLIDQSQNMVGVVSLDQAIRMAED 112
+L + + V ++ +R+ D
Sbjct: 169 TLQKLYLWEKKLYHEVKAEEKMRVDHD 249
>TC16761 similar to GB|AAL32703.1|17065098|AY062625 nucleotide sugar
epimerase-like protein {Arabidopsis thaliana;}, partial
(40%)
Length = 794
Score = 26.2 bits (56), Expect = 6.1
Identities = 20/46 (43%), Positives = 22/46 (47%), Gaps = 7/46 (15%)
Frame = +1
Query: 7 TTVVPVKLKASHFFS---NKLSIP----HSLNLQSSPSFTLPSHAT 45
TT VK A F + N S P HS + SSPS TLPS T
Sbjct: 217 TTATSVKSTAQSFSTLPPNSSSAPPSSSHSSSSSSSPSTTLPSPPT 354
>TC18869 similar to UP|Q9SIR1 (Q9SIR1) At2g25320 protein, partial (8%)
Length = 381
Score = 25.8 bits (55), Expect = 8.0
Identities = 23/102 (22%), Positives = 42/102 (40%), Gaps = 16/102 (15%)
Frame = +1
Query: 139 YELQKKKRDQQKKSAASRMDLKELKMGYNIDQHDYSVRLKAARKFLSDG----------- 187
+ +KK+ +Q + S+++ ++ D V+L A +K L D
Sbjct: 1 FSREKKELTEQVQEIESQLEW------LRSERDDEKVKLSAEKKVLQDRLHDADTQLSQL 162
Query: 188 -----DKVKVIVNLKGREKEFRNNAIELIRRFQNDVGKLATE 224
D++K +V K E NA +RF ++ + ATE
Sbjct: 163 KSRKRDELKKVVKEKNALAERLKNAEAARKRFDEELKRFATE 288
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.130 0.350
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,156,657
Number of Sequences: 28460
Number of extensions: 33550
Number of successful extensions: 198
Number of sequences better than 10.0: 23
Number of HSP's better than 10.0 without gapping: 197
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of query: 266
length of database: 4,897,600
effective HSP length: 89
effective length of query: 177
effective length of database: 2,364,660
effective search space: 418544820
effective search space used: 418544820
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC140546.3