BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140546.1 - phase: 0
(720 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15351 similar to UP|Q8H179 (Q8H179) Eukaryotic initiation fact... 538 e-153
AV409928 230 7e-61
TC17593 similar to UP|Q8H0T8 (Q8H0T8) Eukaryotic initiation fact... 200 6e-52
TC15269 weakly similar to UP|Q8H179 (Q8H179) Eukaryotic initiati... 167 5e-42
TC15268 similar to UP|Q8H0T8 (Q8H0T8) Eukaryotic initiation fact... 155 2e-38
TC8668 similar to UP|Q8W4Q4 (Q8W4Q4) AT4g24800/F6I7_10, partial ... 61 5e-10
TC8815 weakly similar to UP|BAC98352 (BAC98352) Eukaryotic trans... 53 1e-07
TC11306 similar to UP|Q8H0T8 (Q8H0T8) Eukaryotic initiation fact... 46 2e-05
AI967624 42 3e-04
TC17467 similar to UP|Q8LDN5 (Q8LDN5) Topoisomerase-like protein... 39 0.003
TC11571 similar to UP|Q940U9 (Q940U9) AT5g17440/K3M16_10, partia... 34 0.071
TC7927 similar to UP|Q93YR3 (Q93YR3) HSP associated protein like... 33 0.12
TC16308 similar to GB|AAM16172.1|20334832|AY094016 At2g39720/T5I... 32 0.46
TC9626 weakly similar to UP|Q8LEE9 (Q8LEE9) Arabinogalactan prot... 30 1.0
BI420016 30 1.3
AV425945 29 2.3
TC16875 weakly similar to UP|Q7SEE8 (Q7SEE8) Predicted protein, ... 29 2.3
TC18762 similar to UP|Q9VVG2 (Q9VVG2) CG13731-PA, partial (3%) 26 2.6
BP064327 29 3.0
BP062727 28 3.9
>TC15351 similar to UP|Q8H179 (Q8H179) Eukaryotic initiation factor 4,
eIF4-like protein, partial (26%)
Length = 1451
Score = 538 bits (1385), Expect = e-153
Identities = 273/353 (77%), Positives = 304/353 (85%), Gaps = 3/353 (0%)
Frame = +3
Query: 371 WVPRREEIKAKTISEIHDEAEKNLGLRPGATAGMRNTR--VTGVQGNTGAGGFPIARPGT 428
W+PRREEIKAKTI+EIH EAEKNLGLRPGATA MRN R V+G GNTG GGFP RPGT
Sbjct: 3 WIPRREEIKAKTITEIHSEAEKNLGLRPGATASMRNLRGVVSGAPGNTGPGGFP--RPGT 176
Query: 429 GGLMPGMPGARKMPGMPGIDNDNWEVPRTRSMPRGDMSGAQTGGRGQSPYLSKPSVINSK 488
GGLMPGMPG RKMPGMPGIDNDNWEVPRTRSM RGD+SG Q GGRGQ+P++SK + +N+K
Sbjct: 177 GGLMPGMPGTRKMPGMPGIDNDNWEVPRTRSMSRGDVSGMQVGGRGQAPFVSKSTALNTK 356
Query: 489 LLPQGSSGLISGKNSALVLGGGTPSALPSNIVSGTEPAPQIPSPVKPVSAASPEKPQAPA 548
LLPQGS GLISG+NSALV GGG PSA PSNI EPAP+IPSPVKP+ A PQAPA
Sbjct: 357 LLPQGSGGLISGRNSALVHGGGAPSARPSNIGLSAEPAPEIPSPVKPIPAVP--SPQAPA 530
Query: 549 VKLNIDDLHRKTVSLLEEYFNVRLLDEALQCVEELKAPTYHPEVVKEAISLGLDKSPPRV 608
KLN DLHRKTVSLLEEYF+VR+LDEALQCVEELKAP YH EVVKEAI+L LDKSPP V
Sbjct: 531 AKLNTADLHRKTVSLLEEYFSVRILDEALQCVEELKAPAYHHEVVKEAIALALDKSPPCV 710
Query: 609 EPVANLIEYLFTKKILTARDIGTGCLLFASLLDDIGIDLPKAPNNFGEIIGKLVLSAGLD 668
EPVA L+E+LF KKIL+A DIGTGCL F S LDDIGIDLPKAP+NFGEIIGKL+L+ GLD
Sbjct: 711 EPVAKLLEHLFKKKILSASDIGTGCLSFGSTLDDIGIDLPKAPSNFGEIIGKLILAGGLD 890
Query: 669 FKVVKEILKKVGDDYFQKAIFNSAVQVI-SSASGQAVLDSQASDIEACQALFE 720
FKVV EILKKV DD+FQKA F+SA+QVI SSASGQAV+DSQASD+EAC+++ +
Sbjct: 891 FKVVSEILKKVEDDWFQKAFFDSALQVITSSASGQAVVDSQASDVEACRSVLK 1049
>AV409928
Length = 414
Score = 230 bits (586), Expect = 7e-61
Identities = 113/135 (83%), Positives = 126/135 (92%)
Frame = +2
Query: 235 AFEGAGKLREELAQMTSPEQETERRDKDRLVKIRTLGNIRLIGELLKQKMVPERIVHHIV 294
AFEGA KLREEL QMT+PEQE ERRDK+RL+K+RTLGNIRLIGELLKQKMVPE+IVHHIV
Sbjct: 5 AFEGAEKLREELRQMTAPEQEMERRDKERLLKLRTLGNIRLIGELLKQKMVPEKIVHHIV 184
Query: 295 QELLGAADSNVCPAEENVEAICHFFNTIGKQLDESPKSRRINDMYFGRLKELSTNPQLAP 354
QELLG +DS CPAEENVEAIC FFNTIGKQLDESPKSR IND+YF RLK+L++NPQLAP
Sbjct: 185 QELLGTSDSKECPAEENVEAICQFFNTIGKQLDESPKSRHINDVYFIRLKDLASNPQLAP 364
Query: 355 RMKFMVRDVIDLRAS 369
R++FMVRDVIDLRA+
Sbjct: 365 RLRFMVRDVIDLRAN 409
>TC17593 similar to UP|Q8H0T8 (Q8H0T8) Eukaryotic initiation factor 4,
eIF4-like protein, partial (19%)
Length = 704
Score = 200 bits (509), Expect = 6e-52
Identities = 103/164 (62%), Positives = 125/164 (75%), Gaps = 9/164 (5%)
Frame = +1
Query: 2 GDSQFESRERVRYTKEELLHIRETLEETPEDILKLRHDIDAELFGEDQSWGRVENNPPTQ 61
GDS+FE RERVRYT+++LL +RE +E P+ ILK + I+AEL GEDQSW R E+NPP Q
Sbjct: 229 GDSRFEGRERVRYTRDQLLELREVVE-VPDAILKAKQHIEAELLGEDQSWSRTESNPPPQ 405
Query: 62 IQNRYSEPDNRDWRGRSAQPPANADERSWDNIKENREFGNTSQVNRQDQPR--------- 112
QNRYSEPD+RDWRGR+ Q P N D+RSW+N++++ + SQVNRQDQ
Sbjct: 406 SQNRYSEPDSRDWRGRTGQLPPNPDDRSWENLRKH----DASQVNRQDQLNSQFARAQIS 573
Query: 113 TNQGGGPAPTLVKAEVPWSARRGTLSDKDRVLKTVKGILNKLTP 156
+NQGGG + TL KAEVPWS RR TLS+KDRVLKTVKGILNKLTP
Sbjct: 574 SNQGGG-SNTLAKAEVPWSVRRETLSEKDRVLKTVKGILNKLTP 702
>TC15269 weakly similar to UP|Q8H179 (Q8H179) Eukaryotic initiation factor
4, eIF4-like protein, partial (10%)
Length = 733
Score = 167 bits (423), Expect = 5e-42
Identities = 83/111 (74%), Positives = 98/111 (87%)
Frame = +2
Query: 610 PVANLIEYLFTKKILTARDIGTGCLLFASLLDDIGIDLPKAPNNFGEIIGKLVLSAGLDF 669
PV NL+EYLF KKILT DIG+GCL F S LDDIGIDLP+AP+NFGEIIGK++L+ GLDF
Sbjct: 2 PVXNLLEYLFIKKILTNGDIGSGCLSFGSQLDDIGIDLPQAPSNFGEIIGKVILAGGLDF 181
Query: 670 KVVKEILKKVGDDYFQKAIFNSAVQVISSASGQAVLDSQASDIEACQALFE 720
KVVKEIL+K+ DD FQ+A+F+SAV VISSASGQAVL+SQ SDIEACQ+L +
Sbjct: 182 KVVKEILQKMDDDLFQRAVFDSAVGVISSASGQAVLESQKSDIEACQSLLK 334
>TC15268 similar to UP|Q8H0T8 (Q8H0T8) Eukaryotic initiation factor 4,
eIF4-like protein, partial (7%)
Length = 691
Score = 155 bits (393), Expect = 2e-38
Identities = 78/106 (73%), Positives = 92/106 (86%)
Frame = +3
Query: 615 IEYLFTKKILTARDIGTGCLLFASLLDDIGIDLPKAPNNFGEIIGKLVLSAGLDFKVVKE 674
+EYLF KKILT DIG+ CL F S LDDIGIDLP AP+NFGEIIGK++L+ GLDFKVVKE
Sbjct: 3 LEYLFIKKILTNGDIGSXCLSFGSQLDDIGIDLPXAPSNFGEIIGKVILAGGLDFKVVKE 182
Query: 675 ILKKVGDDYFQKAIFNSAVQVISSASGQAVLDSQASDIEACQALFE 720
IL+K+ DD FQ+A+F+SAV VISSASGQAVL+SQ SDIEACQ+L +
Sbjct: 183 ILQKMDDDLFQRAVFDSAVGVISSASGQAVLESQKSDIEACQSLLK 320
>TC8668 similar to UP|Q8W4Q4 (Q8W4Q4) AT4g24800/F6I7_10, partial (39%)
Length = 1216
Score = 61.2 bits (147), Expect = 5e-10
Identities = 31/103 (30%), Positives = 58/103 (56%)
Frame = +3
Query: 553 IDDLHRKTVSLLEEYFNVRLLDEALQCVEELKAPTYHPEVVKEAISLGLDKSPPRVEPVA 612
++D K + LLEEY + ++ EA QC+ +L ++ EVVK+A+ + ++K R +
Sbjct: 519 VEDAKDKIMKLLEEYESSGVVSEACQCIRDLGMSFFNHEVVKKALVMAMEKQNDR---ML 689
Query: 613 NLIEYLFTKKILTARDIGTGCLLFASLLDDIGIDLPKAPNNFG 655
+L++ F++ ++T + G LDD+ +D+P A FG
Sbjct: 690 DLLQECFSEGLITIHQMTQGFTRVKDSLDDLALDIPNAKEKFG 818
Score = 48.9 bits (115), Expect = 3e-06
Identities = 28/103 (27%), Positives = 53/103 (51%)
Frame = +3
Query: 561 VSLLEEYFNVRLLDEALQCVEELKAPTYHPEVVKEAISLGLDKSPPRVEPVANLIEYLFT 620
VS++ EYF + E ++ +E+L AP Y+P +K+ I+L +D+ E + L+ L
Sbjct: 54 VSIIHEYFLSDDIPELIRSLEDLGAPEYNPIFLKKLITLAMDRKNREKEMASVLLSALHI 233
Query: 621 KKILTARDIGTGCLLFASLLDDIGIDLPKAPNNFGEIIGKLVL 663
+I + DI G ++ +D +D+ A N + + V+
Sbjct: 234 -EIFSTEDIVNGFVMLLESAEDTALDILDASNELALFLARAVI 359
>TC8815 weakly similar to UP|BAC98352 (BAC98352) Eukaryotic translation
initiation factor 4G, partial (11%)
Length = 1039
Score = 53.1 bits (126), Expect = 1e-07
Identities = 41/189 (21%), Positives = 79/189 (41%), Gaps = 17/189 (8%)
Frame = +2
Query: 534 KPVSAASPEKPQAPAVKLNIDD--------LHRKTVSLLEEYFNVRLLDEALQCVEELKA 585
+PV + P + Q V N L ++S + EY++ R ++E CV++L +
Sbjct: 29 RPVPTSPPAQLQGTLVSQNASSDSEAFPERLQGLSLSAIREYYSARDVNEVALCVKDLNS 208
Query: 586 PTYHPEVVKEAISLGLDKSPPRVEPVANLIEYLFTKK--ILTARDIGTGCLLFASLLDDI 643
P++HP +V + ++ + +A L+ L + L + G L+D
Sbjct: 209 PSFHPSMVSLWVIDSFERKDTERDLLAKLLVNLVKSQNGTLNQAQLVKGFESALRTLEDA 388
Query: 644 GIDLPKAPNNFGEIIGKLVLSAGLDFKVVKEILKKVGDD-------YFQKAIFNSAVQVI 696
D PKAP G I K++ + + + +++ G++ + S ++ I
Sbjct: 389 VNDAPKAPEFLGRIFAKVITESVVSLNEIGQLIHDGGEEPESLLQVGLAADVLGSTLEAI 568
Query: 697 SSASGQAVL 705
G AVL
Sbjct: 569 RLEKGDAVL 595
>TC11306 similar to UP|Q8H0T8 (Q8H0T8) Eukaryotic initiation factor 4,
eIF4-like protein, partial (6%)
Length = 550
Score = 45.8 bits (107), Expect = 2e-05
Identities = 20/38 (52%), Positives = 31/38 (80%)
Frame = -1
Query: 2 GDSQFESRERVRYTKEELLHIRETLEETPEDILKLRHD 39
GDS+ E RER+RYT+++LL +RE + ET +D+LK++ D
Sbjct: 112 GDSRLEGRERIRYTRDQLLQLREAV-ETLDDMLKIKQD 2
>AI967624
Length = 221
Score = 42.0 bits (97), Expect = 3e-04
Identities = 21/42 (50%), Positives = 29/42 (69%)
Frame = +3
Query: 275 LIGELLKQKMVPERIVHHIVQELLGAADSNVCPAEENVEAIC 316
LIGEL K++M+ ERI+H ++ LLG P EE+VEA+C
Sbjct: 18 LIGELYKKRMLTERIMHECIKTLLGQYQD---PDEEDVEALC 134
>TC17467 similar to UP|Q8LDN5 (Q8LDN5) Topoisomerase-like protein, partial
(25%)
Length = 533
Score = 38.9 bits (89), Expect = 0.003
Identities = 20/68 (29%), Positives = 35/68 (51%)
Frame = +3
Query: 535 PVSAASPEKPQAPAVKLNIDDLHRKTVSLLEEYFNVRLLDEALQCVEELKAPTYHPEVVK 594
P A E+ + ++++ +K LL+EY + EA +C+ EL +H EVVK
Sbjct: 267 PHHAELVERRWGGTTHITVEEVKKKIADLLKEYVDSGDTLEACRCIRELGVSFFHHEVVK 446
Query: 595 EAISLGLD 602
A+ L ++
Sbjct: 447 RALVLAME 470
>TC11571 similar to UP|Q940U9 (Q940U9) AT5g17440/K3M16_10, partial (67%)
Length = 1011
Score = 34.3 bits (77), Expect = 0.071
Identities = 35/117 (29%), Positives = 52/117 (43%)
Frame = +3
Query: 501 KNSALVLGGGTPSALPSNIVSGTEPAPQIPSPVKPVSAASPEKPQAPAVKLNIDDLHRKT 560
K S L+L A + S +P P P P+ P+ A +P+ P + I++ RK
Sbjct: 642 KQSVLLL-----DAFNKDRASLPQPLPN-PPPLAPLPATAPD----PQTQEMINEKMRKA 791
Query: 561 VSLLEEYFNVRLLDEALQCVEELKAPTYHPEVVKEAISLGLDKSPPRVEPVANLIEY 617
L E+ L+DEA + +EE +A L K PPR EPV + +Y
Sbjct: 792 EELGEQ----GLVDEAKKAMEEAEA---------------LKKLPPRQEPVLDSSKY 905
>TC7927 similar to UP|Q93YR3 (Q93YR3) HSP associated protein like, partial
(56%)
Length = 1116
Score = 33.5 bits (75), Expect = 0.12
Identities = 30/95 (31%), Positives = 33/95 (34%), Gaps = 2/95 (2%)
Frame = +1
Query: 427 GTGGLMPGMPGARKMPGMPGIDNDNWEVPRTRSMPRGDMSGAQTGG--RGQSPYLSKPSV 484
G G PGMPG GMPG P G M G GG G P P
Sbjct: 424 GMPGEFPGMPGGFPGGGMPG------------GFPGGGMPGGFPGGGMPGGFPGGGMPGG 567
Query: 485 INSKLLPQGSSGLISGKNSALVLGGGTPSALPSNI 519
+P G G G GGG P +P N+
Sbjct: 568 FPGGGMPGGFPG---GGMPGGFPGGGMPGGVPGNV 663
>TC16308 similar to GB|AAM16172.1|20334832|AY094016 At2g39720/T5I7.2
{Arabidopsis thaliana;}, partial (13%)
Length = 640
Score = 31.6 bits (70), Expect = 0.46
Identities = 21/60 (35%), Positives = 26/60 (43%)
Frame = -1
Query: 458 RSMPRGDMSGAQTGGRGQSPYLSKPSVINSKLLPQGSSGLISGKNSALVLGGGTPSALPS 517
RS P S GGRG+ P S S +SKLLP S+ S + G LP+
Sbjct: 460 RSNPEPSKSSDMVGGRGRRPVPSPSS*NSSKLLPSLSAPAASSPGPRSTITGLNGERLPA 281
>TC9626 weakly similar to UP|Q8LEE9 (Q8LEE9) Arabinogalactan protein-like,
partial (24%)
Length = 605
Score = 30.4 bits (67), Expect = 1.0
Identities = 22/77 (28%), Positives = 38/77 (48%), Gaps = 2/77 (2%)
Frame = +1
Query: 511 TPSALPSNIVSGTEPAPQIPSPVKPVSAASPEKPQAPAVKLNIDDLHRKTVSLLEEYFN- 569
+P+A P + PA P+P KP+ + P+ P + + +I + RK S FN
Sbjct: 139 SPAASPKKSPAKPSPASLAPAPAKPLVPSLPQSPSSDSSGQDIIKILRKAKS-----FNT 303
Query: 570 -VRLLDEALQCVEELKA 585
+RLL + Q + ++ A
Sbjct: 304 LIRLL-KTTQIINQVNA 351
>BI420016
Length = 559
Score = 30.0 bits (66), Expect = 1.3
Identities = 17/48 (35%), Positives = 23/48 (47%)
Frame = +2
Query: 500 GKNSALVLGGGTPSALPSNIVSGTEPAPQIPSPVKPVSAASPEKPQAP 547
G S + TPS PS+ P+P P+P P+S +SP P P
Sbjct: 203 GNPSPKPVPASTPSP-PSSPTLTPPPSPPSPTPNAPLSPSSPPSPPTP 343
>AV425945
Length = 415
Score = 29.3 bits (64), Expect = 2.3
Identities = 12/43 (27%), Positives = 20/43 (45%)
Frame = +3
Query: 77 RSAQPPANADERSWDNIKENREFGNTSQVNRQDQPRTNQGGGP 119
R+ P N D+R W +EN+ F + + R +P + P
Sbjct: 9 RNHDGPCNGDQRRWMRWQENQSFSHRRRGTRPPEPALTRRASP 137
>TC16875 weakly similar to UP|Q7SEE8 (Q7SEE8) Predicted protein, partial
(3%)
Length = 459
Score = 29.3 bits (64), Expect = 2.3
Identities = 19/47 (40%), Positives = 23/47 (48%)
Frame = +3
Query: 509 GGTPSALPSNIVSGTEPAPQIPSPVKPVSAASPEKPQAPAVKLNIDD 555
G TP+A +NI SG E A SPV E P+A V +DD
Sbjct: 303 GQTPAAAAANIDSGDEEADADSSPV-----PEAEAPEAVPVTAPVDD 428
>TC18762 similar to UP|Q9VVG2 (Q9VVG2) CG13731-PA, partial (3%)
Length = 759
Score = 25.8 bits (55), Expect(2) = 2.6
Identities = 23/83 (27%), Positives = 32/83 (37%), Gaps = 12/83 (14%)
Frame = +1
Query: 540 SPEKPQAPAVKLNIDDLHRKTVSLLEEYFNVRL-----LDEALQCVEELKAPTYHPEVVK 594
SP K + D H S+L+ F + L + LQC L A YH +VK
Sbjct: 319 SPLLNSVAVEKDSSDPYHDFRHSMLQMIFEKEIDSEEDLQDLLQCFLHLNAACYHHVIVK 498
Query: 595 -------EAISLGLDKSPPRVEP 610
+A S + +P EP
Sbjct: 499 AFNEICEDAFSDKVSTAPATTEP 567
Score = 21.6 bits (44), Expect(2) = 2.6
Identities = 9/24 (37%), Positives = 11/24 (45%)
Frame = +2
Query: 512 PSALPSNIVSGTEPAPQIPSPVKP 535
PS PS + P P P+P P
Sbjct: 194 PSPSPSTTKTKNSPPPPSPTPKPP 265
>BP064327
Length = 522
Score = 28.9 bits (63), Expect = 3.0
Identities = 14/27 (51%), Positives = 16/27 (58%)
Frame = +3
Query: 294 VQELLGAADSNVCPAEENVEAICHFFN 320
VQ+L G+A S VC N E HFFN
Sbjct: 411 VQKLKGSAASRVCDESVNCERQNHFFN 491
>BP062727
Length = 478
Score = 28.5 bits (62), Expect = 3.9
Identities = 11/35 (31%), Positives = 17/35 (48%)
Frame = +2
Query: 62 IQNRYSEPDNRDWRGRSAQPPANADERSWDNIKEN 96
I+N+ +N DW +Q A +W+N K N
Sbjct: 86 IKNKLESTNNHDWLNCDSQSCRGARAAAWENFKNN 190
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.314 0.135 0.388
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,796,763
Number of Sequences: 28460
Number of extensions: 143208
Number of successful extensions: 744
Number of sequences better than 10.0: 55
Number of HSP's better than 10.0 without gapping: 683
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 720
length of query: 720
length of database: 4,897,600
effective HSP length: 97
effective length of query: 623
effective length of database: 2,136,980
effective search space: 1331338540
effective search space used: 1331338540
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 59 (27.3 bits)
Medicago: description of AC140546.1