BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140033.2 - phase: 0
(352 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC16724 similar to GB|BAB20599.1|12060424|AB049070 AtNAC3 {Arabi... 167 2e-42
BP050825 159 5e-40
TC16322 homologue to UP|Q9SQL0 (Q9SQL0) Jasmonic acid 2, partial... 155 1e-38
BP038107 155 1e-38
TC16783 similar to UP|AAR88435 (AAR88435) NAC domain protein, pa... 150 4e-37
TC18158 similar to UP|Q84TD6 (Q84TD6) At3g04070, partial (34%) 150 4e-37
TC19684 similar to UP|AAR88435 (AAR88435) NAC domain protein, pa... 108 6e-33
TC13201 similar to UP|Q8LRL6 (Q8LRL6) Nam-like protein 9, partia... 117 3e-27
AV428075 100 5e-22
TC12159 similar to UP|Q9M4U3 (Q9M4U3) NAC1, partial (21%) 82 1e-16
TC20162 similar to UP|Q8LKN6 (Q8LKN6) Nam-like protein 18, parti... 72 2e-13
TC9181 similar to UP|Q9FY93 (Q9FY93) NAM-like protein (AT5g13180... 67 3e-12
CB826743 65 2e-11
TC10464 weakly similar to UP|Q8L8G0 (Q8L8G0) Nam-like protein 1,... 64 3e-11
TC14586 similar to UP|Q9FY93 (Q9FY93) NAM-like protein (AT5g1318... 61 2e-10
AU252221 50 7e-07
CB829660 47 4e-06
TC9163 similar to UP|Q9AT45 (Q9AT45) ADP-glucose pyrophosphoryla... 40 1e-05
CB827481 39 0.001
AV428176 39 0.001
>TC16724 similar to GB|BAB20599.1|12060424|AB049070 AtNAC3 {Arabidopsis
thaliana;}, partial (53%)
Length = 615
Score = 167 bits (423), Expect = 2e-42
Identities = 81/167 (48%), Positives = 105/167 (62%)
Frame = +2
Query: 22 GVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMNKCEPWDLPEMAKMGETEWYFFCVR 81
G RFFPTDEEL+ QYL +KV F IAE+D+ K +PW LP A GE EWYFF R
Sbjct: 143 GFRFFPTDEELLVQYLCRKVAGYHFSLPIIAEIDLYKFDPWVLPGKAIFGEKEWYFFSPR 322
Query: 82 DKKYPTGQRTNRATNAGYWKATGKDKEIYKGNSLIGMKKTLVFYKGRAPRGEKSNWVMHE 141
D+KYP G R NR +GYWKATG DK I +G+KK LVFY G+AP+G K+NW+MHE
Sbjct: 323 DRKYPNGSRPNRVAGSGYWKATGTDKVITTEGRKVGIKKALVFYIGKAPKGTKTNWIMHE 502
Query: 142 YRLEGNSLSKHNIFPEHNLFPEHNLSTHGMSEWVVTRVFEKRNCGKK 188
YRL +S +H L + + EWV+ R+++K++ +K
Sbjct: 503 YRLLDSS-------------RKHKLGSSRLDEWVLCRIYKKKSSTQK 604
>BP050825
Length = 539
Score = 159 bits (403), Expect = 5e-40
Identities = 81/165 (49%), Positives = 103/165 (62%), Gaps = 2/165 (1%)
Frame = +2
Query: 22 GVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMNKCEPWDLPEMAKMGETEWYFFCVR 81
G RF PTDEEL+ YL KK IAEVD+ K +PW+LP A GE EWYFF R
Sbjct: 47 GFRFHPTDEELVVHYLKKKAASVPLPVAIIAEVDLYKFDPWELPAKATFGEQEWYFFSPR 226
Query: 82 DKKYPTGQRTNRATNAGYWKATGKDKEIY--KGNSLIGMKKTLVFYKGRAPRGEKSNWVM 139
D+KYP G R NRA +GYWKATG DK + G +G+KK LVFY G+ PRG K+NW+M
Sbjct: 227 DRKYPNGARPNRAATSGYWKATGTDKPVLTSNGTQKVGVKKALVFYGGKPPRGIKTNWIM 406
Query: 140 HEYRLEGNSLSKHNIFPEHNLFPEHNLSTHGMSEWVVTRVFEKRN 184
HEYRL N +N P +L + N + + +WV+ R+++K N
Sbjct: 407 HEYRLADN--KPNNRPPGCDLGNKKN--SLRLDDWVLCRIYKKSN 529
>TC16322 homologue to UP|Q9SQL0 (Q9SQL0) Jasmonic acid 2, partial (40%)
Length = 510
Score = 155 bits (392), Expect = 1e-38
Identities = 70/122 (57%), Positives = 86/122 (70%)
Frame = +1
Query: 22 GVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMNKCEPWDLPEMAKMGETEWYFFCVR 81
G RF+PTDEEL+ QYL +KV ++F IAE+D+ K +PW LP A GE EWYFF R
Sbjct: 145 GFRFYPTDEELLVQYLCRKVAGHNFTLPIIAEIDLYKFDPWVLPSKAIFGEKEWYFFSPR 324
Query: 82 DKKYPTGQRTNRATNAGYWKATGKDKEIYKGNSLIGMKKTLVFYKGRAPRGEKSNWVMHE 141
D+KYP G R NR +GYWKATG DK I +G+KK LVFY G+AP+G K+NW+MHE
Sbjct: 325 DRKYPNGSRPNRVAGSGYWKATGTDKIITTEGRKVGIKKALVFYVGKAPKGTKTNWIMHE 504
Query: 142 YR 143
YR
Sbjct: 505 YR 510
>BP038107
Length = 469
Score = 155 bits (391), Expect = 1e-38
Identities = 73/134 (54%), Positives = 91/134 (67%)
Frame = +3
Query: 11 KMENEKVKFDAGVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMNKCEPWDLPEMAKM 70
KM +E +++ G RF PTDEEL+ YL +K IAE+D+ K +PWDLP +A
Sbjct: 42 KMASE-LQWPPGFRFHPTDEELVLHYLCRKCASQPIAVPIIAEIDLYKYDPWDLPGLASY 218
Query: 71 GETEWYFFCVRDKKYPTGQRTNRATNAGYWKATGKDKEIYKGNSLIGMKKTLVFYKGRAP 130
GE EWYFF RD+KYP G R NRA GYWKATG DK I +G+KK LVFY G+AP
Sbjct: 219 GEKEWYFFSPRDRKYPNGSRPNRAAGTGYWKATGADKPIGHPKP-VGIKKALVFYAGKAP 395
Query: 131 RGEKSNWVMHEYRL 144
+G+K+NW+MHEYRL
Sbjct: 396 KGDKTNWIMHEYRL 437
>TC16783 similar to UP|AAR88435 (AAR88435) NAC domain protein, partial (46%)
Length = 511
Score = 150 bits (378), Expect = 4e-37
Identities = 70/123 (56%), Positives = 83/123 (66%)
Frame = +1
Query: 22 GVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMNKCEPWDLPEMAKMGETEWYFFCVR 81
G RF PTD+EL+ YL K I E+D+ K +PW LP+MA GE EWYFF R
Sbjct: 106 GFRFHPTDDELVKHYLCAKCASQPINVPIIKELDLYKFDPWQLPDMALYGEKEWYFFTPR 285
Query: 82 DKKYPTGQRTNRATNAGYWKATGKDKEIYKGNSLIGMKKTLVFYKGRAPRGEKSNWVMHE 141
D+KYP G R NRA GYWKATG DK I K +L G+KK LVFY G+AP+G K+NW+MHE
Sbjct: 286 DRKYPNGSRPNRAAGTGYWKATGADKPIGKPKTL-GIKKALVFYAGKAPKGVKTNWIMHE 462
Query: 142 YRL 144
YRL
Sbjct: 463 YRL 471
>TC18158 similar to UP|Q84TD6 (Q84TD6) At3g04070, partial (34%)
Length = 476
Score = 150 bits (378), Expect = 4e-37
Identities = 74/130 (56%), Positives = 85/130 (64%), Gaps = 7/130 (5%)
Frame = +3
Query: 22 GVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMNKCEPWDLPEMAKMGETEWYFFCVR 81
G RF PTDEELI YL KKV IAEVD+ K +PW+LP A GE EWYFF R
Sbjct: 87 GFRFHPTDEELILHYLRKKVASIPLPVSIIAEVDIYKLDPWELPGKALFGEKEWYFFSPR 266
Query: 82 DKKYPTGQRTNRATNAGYWKATGKDKEIY-------KGNSLIGMKKTLVFYKGRAPRGEK 134
D+KYP G R NRA +GYWKATG DK I + IG+KK LVFYKG+ P+G K
Sbjct: 267 DRKYPNGARPNRAAASGYWKATGTDKTIVASLPGGGRAQDSIGVKKALVFYKGKPPKGVK 446
Query: 135 SNWVMHEYRL 144
+NW+MHEYRL
Sbjct: 447 TNWIMHEYRL 476
>TC19684 similar to UP|AAR88435 (AAR88435) NAC domain protein, partial (48%)
Length = 535
Score = 108 bits (269), Expect(2) = 6e-33
Identities = 52/111 (46%), Positives = 70/111 (62%), Gaps = 3/111 (2%)
Frame = +2
Query: 4 NENVSNQKMENE---KVKFDAGVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMNKCE 60
+ +++ Q+ +++ +++ G RF PTDEEL+N YL K SF I E+D+ K +
Sbjct: 56 SSSINQQEFKHQGKGELELPPGFRFHPTDEELVNHYLCTKGAGQSFNYSVIKEIDLYKFD 235
Query: 61 PWDLPEMAKMGETEWYFFCVRDKKYPTGQRTNRATNAGYWKATGKDKEIYK 111
PW LPEM GE EWYFF RD+KYP G R NRA +GY KATG DK + K
Sbjct: 236 PWQLPEMGLDGEKEWYFFSPRDRKYPNGSRPNRAAGSGYGKATGADKPMGK 388
Score = 49.3 bits (116), Expect(2) = 6e-33
Identities = 19/29 (65%), Positives = 25/29 (85%)
Frame = +3
Query: 116 IGMKKTLVFYKGRAPRGEKSNWVMHEYRL 144
+G+KK LV Y G+AP+G K+NW+MHEYRL
Sbjct: 399 LGIKKALVLYVGKAPKGIKTNWIMHEYRL 485
>TC13201 similar to UP|Q8LRL6 (Q8LRL6) Nam-like protein 9, partial (22%)
Length = 465
Score = 117 bits (293), Expect = 3e-27
Identities = 64/138 (46%), Positives = 79/138 (56%), Gaps = 9/138 (6%)
Frame = +3
Query: 4 NENVSNQKMENEKVK------FDAGVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMN 57
N N N N ++ G RF PTDEEL+ YL ++V F I VD+
Sbjct: 48 NPNFPNSPNSNPPIRKSMSSNLSPGFRFHPTDEELVGFYLKRRVTGRLFHGDPIGIVDVY 227
Query: 58 KCEPWDLPEMAKMG--ETEWYFFCVRDKKYPTGQ-RTNRATNAGYWKATGKDKEIYKGNS 114
K EPWDLP ++KM + EWYF+ DKKY G RTNRAT GYWK TGKD+ + N
Sbjct: 228 KYEPWDLPCLSKMKTRDLEWYFYSGLDKKYGKGSSRTNRATEKGYWKTTGKDRPVNHANR 407
Query: 115 LIGMKKTLVFYKGRAPRG 132
+GMKKTLV++ GRA G
Sbjct: 408 TVGMKKTLVYHFGRASTG 461
>AV428075
Length = 310
Score = 100 bits (248), Expect = 5e-22
Identities = 49/113 (43%), Positives = 68/113 (59%), Gaps = 1/113 (0%)
Frame = +2
Query: 71 GETEWYFFCVRDKKYPTGQRTNRATNAGYWKATGKDKEIYKGN-SLIGMKKTLVFYKGRA 129
GE EWYF+ RD+KY G R NR T +GYWKATG D+ I N IG+KKTLVFY G+A
Sbjct: 2 GEKEWYFYVPRDRKYRNGDRPNRVTTSGYWKATGADRMIRTENFRSIGLKKTLVFYSGKA 181
Query: 130 PRGEKSNWVMHEYRLEGNSLSKHNIFPEHNLFPEHNLSTHGMSEWVVTRVFEK 182
P+G +++W+M+EYRL P+H + +E + RV+++
Sbjct: 182 PKGMRTSWIMNEYRL-----------------PQHETERYQKAEISLCRVYKR 289
>TC12159 similar to UP|Q9M4U3 (Q9M4U3) NAC1, partial (21%)
Length = 630
Score = 82.0 bits (201), Expect = 1e-16
Identities = 41/88 (46%), Positives = 53/88 (59%), Gaps = 2/88 (2%)
Frame = +1
Query: 16 KVKFDAGVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMNKCEPWDLPEMA--KMGET 73
K+K G RF P D EL+ YL +KV IAEV++ K PWDLP+ + + G+
Sbjct: 367 KMKLVPGFRFHPMDVELVKFYLKRKVMGRKLPHDVIAEVNVYKHAPWDLPDKSCLRTGDL 546
Query: 74 EWYFFCVRDKKYPTGQRTNRATNAGYWK 101
EWYFF +KKY +G R NRAT G+WK
Sbjct: 547 EWYFFSPTEKKYGSGARMNRATEIGFWK 630
>TC20162 similar to UP|Q8LKN6 (Q8LKN6) Nam-like protein 18, partial (17%)
Length = 623
Score = 71.6 bits (174), Expect = 2e-13
Identities = 32/45 (71%), Positives = 40/45 (88%), Gaps = 1/45 (2%)
Frame = +2
Query: 107 KEIYKGN-SLIGMKKTLVFYKGRAPRGEKSNWVMHEYRLEGNSLS 150
+EI+KG +L+GMKKTLVFY+GRAP+GEK+NWVMHE+RLEG S
Sbjct: 23 REIFKGKGNLVGMKKTLVFYRGRAPKGEKTNWVMHEFRLEGKFAS 157
>TC9181 similar to UP|Q9FY93 (Q9FY93) NAM-like protein
(AT5g13180/T19L5_140), partial (30%)
Length = 519
Score = 67.4 bits (163), Expect = 3e-12
Identities = 34/77 (44%), Positives = 48/77 (62%)
Frame = +1
Query: 12 MENEKVKFDAGVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMNKCEPWDLPEMAKMG 71
++N +++ G RF PTDEEL+ QYL +K+ A I EVD+ K +PWDLP +
Sbjct: 295 VKNGELRLPPGFRFHPTDEELVVQYLKRKIFSCPLPASIIPEVDLCKSDPWDLPGDS--- 465
Query: 72 ETEWYFFCVRDKKYPTG 88
E E YFF +++ KYP G
Sbjct: 466 ERERYFFSMKEAKYPNG 516
>CB826743
Length = 513
Score = 65.1 bits (157), Expect = 2e-11
Identities = 41/110 (37%), Positives = 60/110 (54%)
Frame = +2
Query: 106 DKEIYKGNSLIGMKKTLVFYKGRAPRGEKSNWVMHEYRLEGNSLSKHNIFPEHNLFPEHN 165
D++I G++LIGMKKTLVFY GRAP+G++++WVMHEYR L N P N
Sbjct: 2 DRKIKSGSTLIGMKKTLVFYAGRAPKGKRTHWVMHEYRPTLKELDGTN--P*QN------ 157
Query: 166 LSTHGMSEWVVTRVFEKRNCGKKMNGSKLGRSNSSREEPSNTNAASLWAP 215
+V+ R+F+K N L SN E+ ++T + ++P
Sbjct: 158 -------PYVLCRLFKK-------NDESLEGSNEEVEQIASTPTTASFSP 265
>TC10464 weakly similar to UP|Q8L8G0 (Q8L8G0) Nam-like protein 1, partial
(12%)
Length = 935
Score = 64.3 bits (155), Expect = 3e-11
Identities = 38/104 (36%), Positives = 59/104 (56%)
Frame = +1
Query: 112 GNSLIGMKKTLVFYKGRAPRGEKSNWVMHEYRLEGNSLSKHNIFPEHNLFPEHNLSTHGM 171
G+SLIGMKKTLVFY GRAP+G+++NWVMHEYR L N G
Sbjct: 4 GSSLIGMKKTLVFYHGRAPKGKRTNWVMHEYRPTLQELDGTN---------------PGQ 138
Query: 172 SEWVVTRVFEKRNCGKKMNGSKLGRSNSSREEPSNTNAASLWAP 215
+ +V+ R+F+K++ +++ S E+ ++T A+ ++P
Sbjct: 139 NPYVICRLFKKQDESIEVSIS------GEAEQTASTPMAAKYSP 252
>TC14586 similar to UP|Q9FY93 (Q9FY93) NAM-like protein
(AT5g13180/T19L5_140), partial (18%)
Length = 589
Score = 61.2 bits (147), Expect = 2e-10
Identities = 48/146 (32%), Positives = 70/146 (47%), Gaps = 10/146 (6%)
Frame = +3
Query: 116 IGMKKTLVFYKGRAPRGEKSNWVMHEYRLEGNSLSKHNIFPEHNLFPEHNLSTHG----- 170
+GMKKTLVFY G+ P G +++W+MHEYRL N+ + +T G
Sbjct: 3 VGMKKTLVFYTGKPPHGSRTDWIMHEYRLLTNT---------------NTTTTQGHVQVP 137
Query: 171 MSEWVVTRVFEKRNCGKKMNGSKLGRSNSSREEPSNTNAASL---WAPFLEFSPYNSENK 227
M WV+ R+F KR G K NG EE + N SL A + F + ++NK
Sbjct: 138 MENWVLCRIFLKRRGGVK-NG----------EEEHSFNNISLKAGKAGRVVFYDFLAQNK 284
Query: 228 I--TIPDFSNEFNSFTNPNQSEKPKT 251
I S+ + T+ N+S++ T
Sbjct: 285 INPASSSSSSATSGITDNNESDESST 362
>AU252221
Length = 424
Score = 49.7 bits (117), Expect = 7e-07
Identities = 25/44 (56%), Positives = 28/44 (62%)
Frame = +1
Query: 22 GVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMNKCEPWDLP 65
G RF PTDEELI YL KKV IAEVD+ K +PW+LP
Sbjct: 115 GFRFHPTDEELILHYLRKKVASIPLPVSIIAEVDIYKLDPWELP 246
>CB829660
Length = 517
Score = 47.4 bits (111), Expect = 4e-06
Identities = 23/44 (52%), Positives = 28/44 (63%)
Frame = +1
Query: 22 GVRFFPTDEELINQYLVKKVDDNSFCAIAIAEVDMNKCEPWDLP 65
G RF PTDEELI YL +V + A I EVD+ K +PW+LP
Sbjct: 139 GFRFHPTDEELIVHYLCNQVSSHPSPAPIIPEVDIYKFDPWELP 270
Score = 40.8 bits (94), Expect = 3e-04
Identities = 16/24 (66%), Positives = 18/24 (74%)
Frame = +3
Query: 71 GETEWYFFCVRDKKYPTGQRTNRA 94
GE EWYFF R++KYP G R NRA
Sbjct: 444 GEKEWYFFSPRERKYPNGARPNRA 515
>TC9163 similar to UP|Q9AT45 (Q9AT45) ADP-glucose pyrophosphorylase large
subunit (Glucose-1-phosphate adenylyltransferase)
(ADP-glucose synthase) , partial (5%)
Length = 663
Score = 40.0 bits (92), Expect(2) = 1e-05
Identities = 17/27 (62%), Positives = 22/27 (80%)
Frame = +2
Query: 36 YLVKKVDDNSFCAIAIAEVDMNKCEPW 62
YL +KV D+ F AIAIA+ D++KCEPW
Sbjct: 257 YLPQKVLDSYFYAIAIADADLHKCEPW 337
Score = 25.0 bits (53), Expect(2) = 1e-05
Identities = 11/16 (68%), Positives = 12/16 (74%)
Frame = +3
Query: 18 KFDAGVRFFPTDEELI 33
K D G RF PT+EELI
Sbjct: 204 KKDKGFRFHPTNEELI 251
>CB827481
Length = 556
Score = 39.3 bits (90), Expect = 0.001
Identities = 19/43 (44%), Positives = 28/43 (64%), Gaps = 1/43 (2%)
Frame = +2
Query: 22 GVRFFPTDEELINQYLVKKVDDNSFCAIAIAE-VDMNKCEPWD 63
G RF PTDEEL++ YL +K++ S I E +D+ K +PW+
Sbjct: 227 GFRFHPTDEELVSFYLRRKLEKKSHVFELIKESIDLYKYDPWN 355
>AV428176
Length = 416
Score = 38.9 bits (89), Expect = 0.001
Identities = 19/43 (44%), Positives = 27/43 (62%), Gaps = 1/43 (2%)
Frame = +3
Query: 22 GVRFFPTDEELINQYLVKKVDDNSFCAIAIAE-VDMNKCEPWD 63
G RF PTDEEL+ YL +K++ S I E +D+ K +PW+
Sbjct: 207 GFRFHPTDEELVGFYLRRKLEKKSHVFELIKESIDLYKYDPWN 335
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.130 0.391
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,280,063
Number of Sequences: 28460
Number of extensions: 87399
Number of successful extensions: 373
Number of sequences better than 10.0: 50
Number of HSP's better than 10.0 without gapping: 359
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 363
length of query: 352
length of database: 4,897,600
effective HSP length: 91
effective length of query: 261
effective length of database: 2,307,740
effective search space: 602320140
effective search space used: 602320140
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC140033.2