BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC140031.2 + phase: 0 /pseudo
(484 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP038017 50 1e-06
AV425218 44 6e-05
AV417141 29 1.5
CB828712 29 2.0
TC11884 29 2.0
AV415215 29 2.0
TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) prec... 29 2.0
TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (... 28 2.6
TC14312 similar to UP|Q8J174 (Q8J174) RfeF, partial (7%) 28 3.3
TC14577 similar to GB|AAB97843.1|2811224|AF042668 fimbrin 1 {Ara... 28 3.3
BP028899 28 3.3
TC12467 UP|CAE02597 (CAE02597) Nod-factor receptor 5, complete 28 4.4
TC16846 weakly similar to UP|AAQ63465 (AAQ63465) Fatty acid desa... 28 4.4
BP040501 27 5.7
TC14611 similar to UP|Q8LPB2 (Q8LPB2) Glycine-rich RNA binding p... 27 7.4
TC8286 homologue to GB|AAD14482.1|4249385|T2K10 T2K10.11 {Arabid... 27 7.4
TC16449 similar to UP|Q940R4 (Q940R4) AT4g16560/dl4305c, partial... 27 7.4
BP031976 27 9.7
AV426815 27 9.7
TC18892 similar to UP|Q9M9U5 (Q9M9U5) F6A14.15 protein (At1g1874... 27 9.7
>BP038017
Length = 570
Score = 49.7 bits (117), Expect = 1e-06
Identities = 25/93 (26%), Positives = 42/93 (44%)
Frame = +2
Query: 26 SFFNNFPTVLVMDSTYQTNMYKMPMFEIVGVTSTDLTYSVGFEFVTHEKEENFVWVLQMM 85
S +N F ++ D+ Y+ N Y++P GV G + E E +F W+ +
Sbjct: 293 SAYNYFGDAVIFDTMYRPNQYQVPFAPFTGVNHHGQNVLFGCALLLDESESSFTWLFRTW 472
Query: 86 CKLLTSRMNVPKVVVTDKDTTLMNAVSNVLPES 118
+ R P + TD+D + AV+ V PE+
Sbjct: 473 LSAMNDR--PPVSITTDQDRAIQAAVAQVFPET 565
>AV425218
Length = 419
Score = 43.9 bits (102), Expect = 6e-05
Identities = 31/141 (21%), Positives = 65/141 (45%), Gaps = 1/141 (0%)
Frame = +2
Query: 49 PMFEIVGVTSTDLTYSVGFEFVTHEKEENFVWVLQMMCKLLTSRMNVPKVVVTDKDTTLM 108
P+ +VGV + G ++ E E+FVW+ Q + ++ P+ ++TD +
Sbjct: 5 PLATLVGVNHHGQSVLFGCALLSSEDSESFVWLFQSLLHCMSGVP--PQGIITDHSEAMK 178
Query: 109 NAVSNVLPESFAMNCYFHVQKNVKVRCILDCRYPMAKKDGKEVKHGDVV-KKIMRVW*VI 167
A+ VLP + C ++ K + + + +Y + + V + VV + R W I
Sbjct: 179 KAIETVLPSTRHRWCLSYIMKKLPQKLLGYAQYESIRHHLQNVVYDAVVIDEFERNWKKI 358
Query: 168 IESHPLKNYIQMH*WSSKMFV 188
+E L++ + W +++F+
Sbjct: 359 VEDFGLED----NEWLNELFL 409
>AV417141
Length = 418
Score = 29.3 bits (64), Expect = 1.5
Identities = 15/47 (31%), Positives = 23/47 (48%)
Frame = -1
Query: 415 QLVESHLFGSVLIPKILIVYRHRHQQRHHFLKGNVLVLVKHHVPHFH 461
+ +HL G P++L+ + H H +F N LV H+P FH
Sbjct: 415 EFTPNHLIG----PQVLL-HEHLHHDPRYFGDHNTLVTRAEHLPRFH 290
>CB828712
Length = 532
Score = 28.9 bits (63), Expect = 2.0
Identities = 20/62 (32%), Positives = 29/62 (46%), Gaps = 20/62 (32%)
Frame = -1
Query: 427 IPKILIVYRHRHQQRHH----FLKGNVLVLVK----------------HHVPHFHHLLGF 466
IP I+ + RHRH+ RH+ L+ +L+L K HH+ HHLL +
Sbjct: 250 IPLIISLLRHRHRLRHNLLLIILQARLLLLQKIAQ*LQKKS*TLHYFLHHLYFQHHLLIW 71
Query: 467 QI 468
I
Sbjct: 70 SI 65
>TC11884
Length = 557
Score = 28.9 bits (63), Expect = 2.0
Identities = 21/73 (28%), Positives = 34/73 (45%)
Frame = +1
Query: 283 FYSWLGGYMSRYALGNIVLEEERCRGTLCMDKEICGCV*RKSYGLPCACFVATKIRDDKP 342
FY+ + G++S L + E R + C C+ R+++ LPC C +A R
Sbjct: 193 FYTNIRGFVSNKCLQLLDAEHIRMKSY-----GRCDCLLRETHELPCGCELAGYER---- 345
Query: 343 ILLDEIHLHWNRL 355
I + I+ W RL
Sbjct: 346 ISYEAIYPFWKRL 384
>AV415215
Length = 430
Score = 28.9 bits (63), Expect = 2.0
Identities = 17/59 (28%), Positives = 24/59 (39%)
Frame = +3
Query: 426 LIPKILIVYRHRHQQRHHFLKGNVLVLVKHHVPHFHHLLGFQISLPRFQSLFWIQLLFQ 484
L+P + H H HH HH+ H HH + Q+ L + W LL+Q
Sbjct: 147 LLPPFQTHHHHHHHHHHH-----------HHLHHIHHPIILQL-LRIGELT*WAPLLYQ 287
>TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) precursor,
partial (51%)
Length = 1566
Score = 28.9 bits (63), Expect = 2.0
Identities = 13/30 (43%), Positives = 19/30 (63%)
Frame = -1
Query: 442 HHFLKGNVLVLVKHHVPHFHHLLGFQISLP 471
HH L+ +L L++HH +HH Q+SLP
Sbjct: 1203 HHILESFILCLLRHHEVLWHHQ---QLSLP 1123
>TC13318 similar to UP|CAA20314 (CAA20314) SPBC30B4.01c protein (SPBC3D6.14C
protein) (Fragment), partial (15%)
Length = 543
Score = 28.5 bits (62), Expect = 2.6
Identities = 16/49 (32%), Positives = 21/49 (42%)
Frame = -1
Query: 416 LVESHLFGSVLIPKILIVYRHRHQQRHHFLKGNVLVLVKHHVPHFHHLL 464
L+ H +L+P + H H HH L HH+P HHLL
Sbjct: 372 LLPHHHHHHLLLPP----HHHHHHHHHHHLH-------LHHLPLHHHLL 259
>TC14312 similar to UP|Q8J174 (Q8J174) RfeF, partial (7%)
Length = 622
Score = 28.1 bits (61), Expect = 3.3
Identities = 12/47 (25%), Positives = 23/47 (48%)
Frame = -1
Query: 410 YRKGHQLVESHLFGSVLIPKILIVYRHRHQQRHHFLKGNVLVLVKHH 456
Y H+ V H +++ V H + + HH ++ + LV ++HH
Sbjct: 301 YHH*HKQVSEHNQHHKNCCRMMAVPDHNYPEEHHMIEDHTLVALQHH 161
>TC14577 similar to GB|AAB97843.1|2811224|AF042668 fimbrin 1 {Arabidopsis
thaliana;} , partial (9%)
Length = 704
Score = 28.1 bits (61), Expect = 3.3
Identities = 13/30 (43%), Positives = 17/30 (56%)
Frame = -2
Query: 442 HHFLKGNVLVLVKHHVPHFHHLLGFQISLP 471
H +L G L+ + HFHHLL Q+ LP
Sbjct: 244 HQWLNGKPHCLLNQY--HFHHLLRLQVMLP 161
>BP028899
Length = 474
Score = 28.1 bits (61), Expect = 3.3
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = -3
Query: 90 TSRMNVPKVVVTDKDTTLMNAVSNVLPESFAMNCYFHVQKNVKV 133
T R+NV VVVT K + N ++ + F ++ Y + VKV
Sbjct: 385 TMRLNVTSVVVTKKLVGIPNWAIDIRDDVFNISSYTRIDGKVKV 254
>TC12467 UP|CAE02597 (CAE02597) Nod-factor receptor 5, complete
Length = 2292
Score = 27.7 bits (60), Expect = 4.4
Identities = 17/48 (35%), Positives = 24/48 (49%), Gaps = 11/48 (22%)
Frame = -1
Query: 434 YRHRHQQR---HHFLKGNVLVLVKHH--------VPHFHHLLGFQISL 470
++H H+ R HH L+ + LV H VPH+HHL F + L
Sbjct: 1290 FQHIHKPRNSFHHSLRRHPLVSPDFHDLPSAEFSVPHWHHLP*FSLPL 1147
>TC16846 weakly similar to UP|AAQ63465 (AAQ63465) Fatty acid desaturase,
partial (30%)
Length = 600
Score = 27.7 bits (60), Expect = 4.4
Identities = 12/34 (35%), Positives = 20/34 (58%)
Frame = -1
Query: 433 VYRHRHQQRHHFLKGNVLVLVKHHVPHFHHLLGF 466
VY+ ++R H+ +GN+ ++V HHLL F
Sbjct: 522 VYKEYPRRRRHYKEGNIKARKTNNVNGNHHLLCF 421
>BP040501
Length = 562
Score = 27.3 bits (59), Expect = 5.7
Identities = 9/20 (45%), Positives = 14/20 (70%)
Frame = +1
Query: 238 IGAIVWVIWVLVGRKYLICW 257
+G+ +WV+ VGRK+L W
Sbjct: 379 LGSFIWVLMCKVGRKWLSSW 438
>TC14611 similar to UP|Q8LPB2 (Q8LPB2) Glycine-rich RNA binding protein,
partial (20%)
Length = 760
Score = 26.9 bits (58), Expect = 7.4
Identities = 11/27 (40%), Positives = 13/27 (47%)
Frame = -2
Query: 436 HRHQQRHHFLKGNVLVLVKHHVPHFHH 462
H H Q HH L + H PH+HH
Sbjct: 333 HHHHQSHH------LHISYEHSPHYHH 271
>TC8286 homologue to GB|AAD14482.1|4249385|T2K10 T2K10.11 {Arabidopsis
thaliana;}, partial (6%)
Length = 671
Score = 26.9 bits (58), Expect = 7.4
Identities = 15/37 (40%), Positives = 21/37 (56%)
Frame = +2
Query: 408 EVYRKGHQLVESHLFGSVLIPKILIVYRHRHQQRHHF 444
EVYR ++ ES S +P + H+HQQ+HHF
Sbjct: 260 EVYRPELEIGES----SSSMPAGYHHHHHQHQQQHHF 358
>TC16449 similar to UP|Q940R4 (Q940R4) AT4g16560/dl4305c, partial (18%)
Length = 613
Score = 26.9 bits (58), Expect = 7.4
Identities = 15/53 (28%), Positives = 21/53 (39%)
Frame = +3
Query: 419 SHLFGSVLIPKILIVYRHRHQQRHHFLKGNVLVLVKHHVPHFHHLLGFQISLP 471
SH + VL+P + + + HH LK H H Q+SLP
Sbjct: 114 SHSYQMVLLPLTHSISKSQFNSTHHLLKTTSTRSAARFQHHHHRHQQHQLSLP 272
>BP031976
Length = 292
Score = 26.6 bits (57), Expect = 9.7
Identities = 12/36 (33%), Positives = 22/36 (60%), Gaps = 2/36 (5%)
Frame = -3
Query: 285 SWLGGYMSRYALGNIV--LEEERCRGTLCMDKEICG 318
S + G++ LGN++ L+ ++C+ LCM K + G
Sbjct: 284 SIIKGWLDDQVLGNVMELLQNDKCKLGLCMKKRLHG 177
>AV426815
Length = 391
Score = 26.6 bits (57), Expect = 9.7
Identities = 15/48 (31%), Positives = 25/48 (51%)
Frame = -3
Query: 427 IPKILIVYRHRHQQRHHFLKGNVLVLVKHHVPHFHHLLGFQISLPRFQ 474
IP++ ++ R RH+ RH F V ++ H HH+ ++ PR Q
Sbjct: 212 IPRLTLLRRRRHRSRHRF---KVPTPIRRH----HHI---RLHCPRLQ 99
>TC18892 similar to UP|Q9M9U5 (Q9M9U5) F6A14.15 protein
(At1g18740/F6A14_15), partial (35%)
Length = 547
Score = 26.6 bits (57), Expect = 9.7
Identities = 15/44 (34%), Positives = 21/44 (47%)
Frame = +2
Query: 435 RHRHQQRHHFLKGNVLVLVKHHVPHFHHLLGFQISLPRFQSLFW 478
R+ H Q H+ HH + HHLLG + +F+SL W
Sbjct: 341 RNNHNQIHN----------SHH--NHHHLLGSSSTFGQFRSLSW 436
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.342 0.150 0.517
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,410,205
Number of Sequences: 28460
Number of extensions: 171701
Number of successful extensions: 1676
Number of sequences better than 10.0: 45
Number of HSP's better than 10.0 without gapping: 1611
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1659
length of query: 484
length of database: 4,897,600
effective HSP length: 94
effective length of query: 390
effective length of database: 2,222,360
effective search space: 866720400
effective search space used: 866720400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 39 (22.0 bits)
S2: 57 (26.6 bits)
Medicago: description of AC140031.2