BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139854.7 + phase: 0 /pseudo
(672 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10194 similar to UP|Q8S979 (Q8S979) Auxin response factor 7a (... 30 1.6
BP072692 28 3.7
BP039880 28 4.8
AV422365 28 4.8
BI420757 28 6.3
TC18826 similar to UP|Q9LUW5 (Q9LUW5) DEAD-box RNA helicase-like... 28 6.3
TC11913 weakly similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding p... 25 6.7
TC16562 similar to GB|AAM78073.1|21928089|AY125563 AT4g24660/F22... 27 8.2
AV779201 27 8.2
TC13829 similar to UP|P93321 (P93321) Cdc2MsD protein, partial (... 27 8.2
TC15506 similar to UP|Q9SEE9 (Q9SEE9) Arginine/serine-rich prote... 27 8.2
AV774434 27 8.2
>TC10194 similar to UP|Q8S979 (Q8S979) Auxin response factor 7a (Fragment),
partial (13%)
Length = 1293
Score = 29.6 bits (65), Expect = 1.6
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = -1
Query: 259 IFFGLIQHPLSCLTIFQRFWLWTP 282
I+ G+ HPL + R WLWTP
Sbjct: 177 IYLGICPHPLKLCWWWWRCWLWTP 106
>BP072692
Length = 422
Score = 28.5 bits (62), Expect = 3.7
Identities = 20/56 (35%), Positives = 27/56 (47%)
Frame = +2
Query: 610 PPPRKVPTKGAKKKFDIARSKGKITSTSRIPSSGEVVDSQNPDSQWSPSPTTSSYK 665
PPPR +++ S S+S PS+ + S P S SPSP+TSS K
Sbjct: 167 PPPRP-----HRRQLTATTSSSATPSSSPTPSAAPPLSSP-PSSTPSPSPSTSSSK 316
>BP039880
Length = 576
Score = 28.1 bits (61), Expect = 4.8
Identities = 15/45 (33%), Positives = 25/45 (55%)
Frame = -3
Query: 616 PTKGAKKKFDIARSKGKITSTSRIPSSGEVVDSQNPDSQWSPSPT 660
P+ + KF +IT++S +PSS ++SQ+P + W P T
Sbjct: 415 PSISLRWKFR*QLQAAQITASS-VPSSPAAMNSQHPSTAWPPKLT 284
>AV422365
Length = 489
Score = 28.1 bits (61), Expect = 4.8
Identities = 23/81 (28%), Positives = 38/81 (46%), Gaps = 9/81 (11%)
Frame = -1
Query: 216 MNVNKYGRQIGVTRSRCNI*SRSWRSTTILITQEHNLKVILLKIFFGL---------IQH 266
M++N TR RC I*+ ++ + IT+ ++VIL L I H
Sbjct: 288 MHINHKSATSICTRDRCTI*ACAFFIKLLSITEAIEVEVILWVHIIKLH*FQCSSNNIIH 109
Query: 267 PLSCLTIFQRFWLWTPRTKPT 287
L CL + ++ +L +PR + T
Sbjct: 108 HLHCLALPEKDFLHSPRFRIT 46
>BI420757
Length = 528
Score = 27.7 bits (60), Expect = 6.3
Identities = 24/77 (31%), Positives = 33/77 (42%), Gaps = 9/77 (11%)
Frame = +2
Query: 598 RQLAFPETTLMSPPPRKVP-TKGAKKKFDIARSKGKITSTSRIPSSGE------VVDSQN 650
+QL + PPP V T K F + ++GKIT SR P + V S
Sbjct: 191 QQLQGSVPAVPDPPPVTVKFTDSNLKTFPPSETQGKITGGSRPPRDADDSFSKPVSGSDE 370
Query: 651 PDSQ--WSPSPTTSSYK 665
P Q W + T ++YK
Sbjct: 371 PQQQGGWLHTFTVAAYK 421
>TC18826 similar to UP|Q9LUW5 (Q9LUW5) DEAD-box RNA helicase-like protein,
partial (29%)
Length = 782
Score = 27.7 bits (60), Expect = 6.3
Identities = 21/71 (29%), Positives = 29/71 (40%), Gaps = 1/71 (1%)
Frame = +1
Query: 603 PETTLMSPPPRKVPTKGAKKKFDIARSKGKITSTS-RIPSSGEVVDSQNPDSQWSPSPTT 661
P + +PPP P S G+ TST + P S + +P+S S T
Sbjct: 40 PPSRGAAPPPSSPPQPSPASSVSSPSSPGQDTSTRIQFPCSSAPLSLLSPNSPSLISHTK 219
Query: 662 SSYKRKKGVRS 672
+ K KG RS
Sbjct: 220 KAPKTMKGSRS 252
>TC11913 weakly similar to UP|ABPA_PRUPE (Q9ZRA4) Auxin-binding protein
ABP19a precursor, partial (61%)
Length = 530
Score = 25.0 bits (53), Expect(2) = 6.7
Identities = 7/16 (43%), Positives = 10/16 (61%)
Frame = +3
Query: 486 WVIWVLVGRKYMTCCC 501
W W+++G M CCC
Sbjct: 393 WRAWLVLGLLIMDCCC 440
Score = 20.8 bits (42), Expect(2) = 6.7
Identities = 6/12 (50%), Positives = 8/12 (66%)
Frame = +2
Query: 475 MLC*RNTWTIVW 486
+LC TW +VW
Sbjct: 302 LLCLATTWILVW 337
>TC16562 similar to GB|AAM78073.1|21928089|AY125563 AT4g24660/F22K18_140
{Arabidopsis thaliana;}, partial (29%)
Length = 621
Score = 27.3 bits (59), Expect = 8.2
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = -3
Query: 616 PTKGAKKKFDIARSKGKITSTSRIPSSGEVVDSQNPDSQWSPSPT 660
P + KF +IT++S +PS +SQ P + W P PT
Sbjct: 511 PPVSLRWKFRWQLQAAQITASS-VPSCPAARNSQQPSTAWPPIPT 380
>AV779201
Length = 531
Score = 27.3 bits (59), Expect = 8.2
Identities = 10/18 (55%), Positives = 11/18 (60%)
Frame = +3
Query: 500 CCFSSPLYKHHLVTVFPC 517
CCF PL HL+ FPC
Sbjct: 207 CCFWLPLLLEHLLQSFPC 260
>TC13829 similar to UP|P93321 (P93321) Cdc2MsD protein, partial (43%)
Length = 515
Score = 27.3 bits (59), Expect = 8.2
Identities = 19/63 (30%), Positives = 32/63 (50%)
Frame = +2
Query: 605 TTLMSPPPRKVPTKGAKKKFDIARSKGKITSTSRIPSSGEVVDSQNPDSQWSPSPTTSSY 664
T + P R P K + + ++R K ++ R PS+ ++P S+ SP+P+TSS
Sbjct: 125 TAKSTKPKRNPPAKSSLSRKPVSRWTKK--ASLRPPSA------KSPSSRCSPNPSTSSA 280
Query: 665 KRK 667
K
Sbjct: 281 SSK 289
>TC15506 similar to UP|Q9SEE9 (Q9SEE9) Arginine/serine-rich protein, partial
(24%)
Length = 677
Score = 27.3 bits (59), Expect = 8.2
Identities = 23/89 (25%), Positives = 33/89 (36%), Gaps = 6/89 (6%)
Frame = +2
Query: 590 KLEVKEGLRQLAFPETTLMSPPPRKVPTKGAKKKFDIARSKGKI------TSTSRIPSSG 643
+L G R+ P SPPPR+ + + RS+ I S S P G
Sbjct: 14 RLRGSPGRRRSPPPPPRRRSPPPRRARSPPRRSPIGRRRSRSPIRRPARSNSRSFSPRRG 193
Query: 644 EVVDSQNPDSQWSPSPTTSSYKRKKGVRS 672
+ S +S SP+ R+ RS
Sbjct: 194 RPPVRRGRSSSFSDSPSPRKVSRRSRSRS 280
>AV774434
Length = 354
Score = 27.3 bits (59), Expect = 8.2
Identities = 13/33 (39%), Positives = 20/33 (60%)
Frame = -1
Query: 236 SRSWRSTTILITQEHNLKVILLKIFFGLIQHPL 268
+RSW I++T +V+ L + FG I+HPL
Sbjct: 159 NRSWGVVDIIVTL*ETHRVVDLLLRFGGIKHPL 61
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.351 0.154 0.553
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 13,311,599
Number of Sequences: 28460
Number of extensions: 215814
Number of successful extensions: 2449
Number of sequences better than 10.0: 24
Number of HSP's better than 10.0 without gapping: 2422
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2447
length of query: 672
length of database: 4,897,600
effective HSP length: 96
effective length of query: 576
effective length of database: 2,165,440
effective search space: 1247293440
effective search space used: 1247293440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 38 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC139854.7