BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139841.8 + phase: 0
(422 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP034539 135 2e-32
TC11202 114 2e-26
TC9351 similar to UP|Q9ZUV7 (Q9ZUV7) At2g28150 protein, partial ... 51 4e-07
TC9350 similar to UP|Q9ZUV7 (Q9ZUV7) At2g28150 protein, partial ... 47 5e-06
BP044172 41 3e-04
BP034655 37 0.006
TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, ... 37 0.008
TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) prec... 36 0.014
BP031757 35 0.030
TC14994 similar to UP|O82469 (O82469) Protein phosphatase-2C , p... 34 0.052
TC9623 homologue to UP|Q8H1K8 (Q8H1K8) Stromal ascorbate peroxid... 33 0.089
BP036655 32 0.15
TC10832 weakly similar to UP|Q9SEE9 (Q9SEE9) Arginine/serine-ric... 32 0.15
TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly pr... 32 0.15
AI967639 32 0.26
TC18517 weakly similar to UP|AAQ82688 (AAQ82688) Epa4p, partial ... 32 0.26
TC16003 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcri... 31 0.34
AV410716 31 0.44
TC12137 similar to UP|Q8L7W3 (Q8L7W3) At2g29210/F16P2.41, partia... 31 0.44
AV424050 30 0.57
>BP034539
Length = 423
Score = 135 bits (339), Expect = 2e-32
Identities = 65/77 (84%), Positives = 71/77 (91%)
Frame = -2
Query: 290 SGASSGKDQRFSLVGHYKSRLPRGGGNHVGKEVGTLVEIPDLNRVRLEDKEYFSGSLIET 349
SGA +GKDQ FSLVGHYKSRLPRG GNH GKEVGTL+EIPDL+RVRLEDKEYFSGSLIET
Sbjct: 419 SGAGAGKDQGFSLVGHYKSRLPRGAGNHGGKEVGTLMEIPDLSRVRLEDKEYFSGSLIET 240
Query: 350 KKVEAPAFKRSSSYNAD 366
+KVE P+ KRS+SYNAD
Sbjct: 239 QKVETPSLKRSNSYNAD 189
>TC11202
Length = 537
Score = 114 bits (286), Expect = 2e-26
Identities = 57/72 (79%), Positives = 65/72 (90%)
Frame = +1
Query: 349 TKKVEAPAFKRSSSYNADSSSRLQIMEHEGDMVRAKCIPRKSKTLLTKKEEGTSSSMDSV 408
T+KVE P+ KRS+SYNADS SRLQ++EHEG++VRAKCIPRKSKTL TKKEEGT SMD V
Sbjct: 1 TQKVETPSLKRSNSYNADSGSRLQMIEHEGEVVRAKCIPRKSKTLTTKKEEGT--SMDIV 174
Query: 409 SRSQHGSKRFEG 420
SRSQHGSKRF+G
Sbjct: 175 SRSQHGSKRFDG 210
>TC9351 similar to UP|Q9ZUV7 (Q9ZUV7) At2g28150 protein, partial (7%)
Length = 568
Score = 50.8 bits (120), Expect = 4e-07
Identities = 21/41 (51%), Positives = 27/41 (65%)
Frame = +3
Query: 90 RSYKNGFVWHDLTESDFIYPTQGHDYILKGSEILEHETTAR 130
RSYKNGFVWHDL E D P G+ +LKGSE+ + + +
Sbjct: 51 RSYKNGFVWHDLCEDDKSLPAHGNKNVLKGSELFDKSNSVK 173
>TC9350 similar to UP|Q9ZUV7 (Q9ZUV7) At2g28150 protein, partial (6%)
Length = 577
Score = 47.4 bits (111), Expect = 5e-06
Identities = 19/38 (50%), Positives = 25/38 (65%)
Frame = +3
Query: 93 KNGFVWHDLTESDFIYPTQGHDYILKGSEILEHETTAR 130
KNGFVWHDL E D P+ GH +LKGSE+ + + +
Sbjct: 3 KNGFVWHDLCEDDKSLPSPGHKNVLKGSELFDKSNSVK 116
>BP044172
Length = 235
Score = 41.2 bits (95), Expect = 3e-04
Identities = 21/46 (45%), Positives = 28/46 (60%), Gaps = 2/46 (4%)
Frame = +3
Query: 1 MSVTSSSRGRPGTRETSPERTKVWTEPKPKTPR--KVSVVYYLSRN 44
+ V +R + ++ SPER KVWTE PK + KV V+YYL RN
Sbjct: 96 LHVRMEARMKKYRQQVSPERAKVWTEKSPKYHQSLKVPVIYYLCRN 233
>BP034655
Length = 517
Score = 37.0 bits (84), Expect = 0.006
Identities = 16/38 (42%), Positives = 25/38 (65%)
Frame = +1
Query: 189 LREEDEEQEEEEKEKNDEIENEGETENQNQSTELSREE 226
L ++DEE+EEEE+E+ +E E E E E + + E E+
Sbjct: 130 LSDDDEEEEEEEEEEEEEEEEEEEEEEEEEDEEDDEED 243
Score = 35.8 bits (81), Expect = 0.014
Identities = 22/69 (31%), Positives = 36/69 (51%), Gaps = 6/69 (8%)
Frame = +1
Query: 172 AADASTQTEEKRRRRRVLREEDEEQEEEEKEKNDE-IENEGETENQNQSTELSREEISP- 229
AAD +++ EE+EE+EEEE+E+ +E E++ E E+++ +SP
Sbjct: 115 AADFGLSDDDEEEEEEEEEEEEEEEEEEEEEEEEEDEEDDEEDEDEDYIPVAPSHRVSPN 294
Query: 230 ----PPSDS 234
P SDS
Sbjct: 295 VHQNPTSDS 321
>TC9200 similar to UP|Q8LLE6 (Q8LLE6) RNA-binding protein AKIP1, partial
(31%)
Length = 727
Score = 36.6 bits (83), Expect = 0.008
Identities = 20/65 (30%), Positives = 34/65 (51%)
Frame = +1
Query: 158 EYRVYKSEPLGENIAADASTQTEEKRRRRRVLREEDEEQEEEEKEKNDEIENEGETENQN 217
E V + E + E + + + EE+ EE+ E+EEEE+E+ +E+E E E E+
Sbjct: 178 EAEVQEYEEVEEEVEEEVEEEEEEEEE------EEEGEEEEEEEEEEEEVEAEAEEEDDE 339
Query: 218 QSTEL 222
+L
Sbjct: 340 PIQKL 354
Score = 35.0 bits (79), Expect = 0.023
Identities = 18/58 (31%), Positives = 29/58 (49%)
Frame = +1
Query: 191 EEDEEQEEEEKEKNDEIENEGETENQNQSTELSREEISPPPSDSSPETLGTLMKVDGR 248
EE EE+ EEE E+ +E E E E + + E EE+ + E + L++ G+
Sbjct: 199 EEVEEEVEEEVEEEEEEEEEEEEGEEEEEEEEEEEEVEAEAEEEDDEPIQKLLEPMGK 372
>TC14970 similar to UP|O24294 (O24294) Legumin (Minor small) precursor,
partial (51%)
Length = 1566
Score = 35.8 bits (81), Expect = 0.014
Identities = 19/48 (39%), Positives = 27/48 (55%), Gaps = 1/48 (2%)
Frame = +1
Query: 183 RRRRRVLREEDEEQEEEEKEKNDEIENEGETENQ-NQSTELSREEISP 229
RR RR REEDE+++E+E+E+ GE + + E REE P
Sbjct: 577 RRTRRPSREEDEDEDEDEEERQGGRRGPGERQRHFEREEEEEREERGP 720
>BP031757
Length = 507
Score = 34.7 bits (78), Expect = 0.030
Identities = 20/71 (28%), Positives = 40/71 (56%), Gaps = 4/71 (5%)
Frame = +2
Query: 182 KRRRRRVLREEDEEQE----EEEKEKNDEIENEGETENQNQSTELSREEISPPPSDSSPE 237
KR+R ++L E++E+++ EEE+EK + ++ + E + + E E PP D+ P
Sbjct: 140 KRKRMKLLEEDNEDKDGEDYEEEEEKKKKKKSRKKKEEEEEE-EQEEPESPQPPEDAKP- 313
Query: 238 TLGTLMKVDGR 248
+G ++ G+
Sbjct: 314 -IGDPVRFSGK 343
Score = 28.9 bits (63), Expect = 1.7
Identities = 9/25 (36%), Positives = 22/25 (88%)
Frame = +2
Query: 178 QTEEKRRRRRVLREEDEEQEEEEKE 202
+ EEK+++++ ++++EE+EEE++E
Sbjct: 203 EEEEKKKKKKSRKKKEEEEEEEQEE 277
>TC14994 similar to UP|O82469 (O82469) Protein phosphatase-2C , partial
(89%)
Length = 1672
Score = 33.9 bits (76), Expect = 0.052
Identities = 15/30 (50%), Positives = 21/30 (70%)
Frame = +2
Query: 188 VLREEDEEQEEEEKEKNDEIENEGETENQN 217
+ REE+EE EEEE+E+ +E E E E +N
Sbjct: 152 IAREEEEEVEEEEEEEEEEEEEEEEEAEKN 241
Score = 29.3 bits (64), Expect = 1.3
Identities = 15/34 (44%), Positives = 20/34 (58%)
Frame = +2
Query: 171 IAADASTQTEEKRRRRRVLREEDEEQEEEEKEKN 204
IA + + EE+ EE+EE+EEEE EKN
Sbjct: 152 IAREEEEEVEEEEEEE----EEEEEEEEEEAEKN 241
>TC9623 homologue to UP|Q8H1K8 (Q8H1K8) Stromal ascorbate peroxidase,
partial (42%)
Length = 640
Score = 33.1 bits (74), Expect = 0.089
Identities = 14/30 (46%), Positives = 22/30 (72%)
Frame = -1
Query: 181 EKRRRRRVLREEDEEQEEEEKEKNDEIENE 210
E+R+ + REE+EE+EE EK++ +E E E
Sbjct: 214 EERKHWKEEREEEEEEEENEKKEREEYEEE 125
>BP036655
Length = 556
Score = 32.3 bits (72), Expect = 0.15
Identities = 18/66 (27%), Positives = 33/66 (49%)
Frame = -3
Query: 161 VYKSEPLGENIAADASTQTEEKRRRRRVLREEDEEQEEEEKEKNDEIENEGETENQNQST 220
+++ E L ++ D EE+ V+ E ++ + + E +DE+E E ETE + +
Sbjct: 416 LFEEEGLSDDGDDDEVVDEEEE-----VVEEWSDDDDINDDESDDEVEEESETEIETEKE 252
Query: 221 ELSREE 226
E EE
Sbjct: 251 EEEEEE 234
>TC10832 weakly similar to UP|Q9SEE9 (Q9SEE9) Arginine/serine-rich protein,
partial (30%)
Length = 539
Score = 32.3 bits (72), Expect = 0.15
Identities = 20/50 (40%), Positives = 27/50 (54%)
Frame = -2
Query: 189 LREEDEEQEEEEKEKNDEIENEGETENQNQSTELSREEISPPPSDSSPET 238
LRE E EEEE+EK+ E E+E E +++ REE + PET
Sbjct: 274 LREPPREGEEEEEEKDLERESERERDDE-------REEDGEEEREEEPET 146
Score = 29.6 bits (65), Expect = 0.98
Identities = 16/41 (39%), Positives = 23/41 (56%), Gaps = 3/41 (7%)
Frame = -2
Query: 174 DASTQTEEKRRRRRVLREEDEEQE---EEEKEKNDEIENEG 211
+ + EEK R RE D+E+E EEE+E+ E E +G
Sbjct: 256 EGEEEEEEKDLERESERERDDEREEDGEEEREEEPETEPDG 134
>TC17157 similar to UP|CAD27462 (CAD27462) Nucleosome assembly protein
1-like protein 3, partial (81%)
Length = 1298
Score = 32.3 bits (72), Expect = 0.15
Identities = 14/47 (29%), Positives = 31/47 (65%)
Frame = +2
Query: 191 EEDEEQEEEEKEKNDEIENEGETENQNQSTELSREEISPPPSDSSPE 237
++D+++EEE+ + +DE + EGE +++++S +R P++ PE
Sbjct: 812 DDDDDEEEEDDDDDDEDDEEGEGKSKSKSGSKARPG-KDQPTERPPE 949
>AI967639
Length = 335
Score = 31.6 bits (70), Expect = 0.26
Identities = 18/51 (35%), Positives = 30/51 (58%), Gaps = 3/51 (5%)
Frame = +1
Query: 181 EKRRRRRVLR---EEDEEQEEEEKEKNDEIENEGETENQNQSTELSREEIS 228
+KR++RR + E DEE EEE++ +E+E E ++ + +LS E S
Sbjct: 58 KKRKQRRDISSSSEGDEEDEEEDQGDEYNLEDEEEDDDDDDDDDLSISEDS 210
>TC18517 weakly similar to UP|AAQ82688 (AAQ82688) Epa4p, partial (4%)
Length = 493
Score = 31.6 bits (70), Expect = 0.26
Identities = 15/46 (32%), Positives = 29/46 (62%), Gaps = 3/46 (6%)
Frame = -2
Query: 186 RRVLREEDEEQEEEEKEKNDE---IENEGETENQNQSTELSREEIS 228
R V EE+EE EE+ E++D+ + +EG+ E++ + + EE++
Sbjct: 390 RAVAEEEEEEPGEEDGEEDDDGDGVPDEGDEEDEEEDHGVVAEEVA 253
>TC16003 similar to UP|WR17_ARATH (Q9SJA8) Probable WRKY transcription
factor 17 (WRKY DNA-binding protein 17), partial (20%)
Length = 446
Score = 31.2 bits (69), Expect = 0.34
Identities = 17/35 (48%), Positives = 23/35 (65%), Gaps = 1/35 (2%)
Frame = -3
Query: 169 ENIAADASTQTEEKRRRR-RVLREEDEEQEEEEKE 202
E + + S Q+ R RR R+ EE+EE+EEEEKE
Sbjct: 237 ETVKSVISVQSRLLRSRRWRLWEEEEEEEEEEEKE 133
>AV410716
Length = 424
Score = 30.8 bits (68), Expect = 0.44
Identities = 16/36 (44%), Positives = 22/36 (60%), Gaps = 2/36 (5%)
Frame = +2
Query: 188 VLREEDEE--QEEEEKEKNDEIENEGETENQNQSTE 221
+ REE+EE +EEEE+E+ +E E G STE
Sbjct: 146 IAREEEEEVEEEEEEEEEEEEAEKNGG*SRNRVSTE 253
Score = 28.5 bits (62), Expect = 2.2
Identities = 12/23 (52%), Positives = 15/23 (65%)
Frame = +2
Query: 182 KRRRRRVLREEDEEQEEEEKEKN 204
+ V EE+EE+EEEE EKN
Sbjct: 152 REEEEEVEEEEEEEEEEEEAEKN 220
>TC12137 similar to UP|Q8L7W3 (Q8L7W3) At2g29210/F16P2.41, partial (9%)
Length = 579
Score = 30.8 bits (68), Expect = 0.44
Identities = 21/69 (30%), Positives = 35/69 (50%), Gaps = 9/69 (13%)
Frame = +3
Query: 180 EEKRRRRRVLREEDEEQEEEE---KEKNDEIENEGETE------NQNQSTELSREEISPP 230
EEKRRRR R EE+ E+ K K D I ++ E E + N+ T++ +++
Sbjct: 96 EEKRRRREERRHRREERRAEKLKLKSKTDYISDDEEAERMDDHPSNNEETQVDPKKLEIE 275
Query: 231 PSDSSPETL 239
+ + E+L
Sbjct: 276 LRNKALESL 302
>AV424050
Length = 274
Score = 30.4 bits (67), Expect = 0.57
Identities = 14/34 (41%), Positives = 22/34 (64%)
Frame = -2
Query: 182 KRRRRRVLREEDEEQEEEEKEKNDEIENEGETEN 215
+RRRRR E+EEEE+E+ +E E++ + N
Sbjct: 120 RRRRRRSWVRLRVEEEEEEEEEEEESESQNDKRN 19
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.309 0.128 0.359
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,106,065
Number of Sequences: 28460
Number of extensions: 100655
Number of successful extensions: 830
Number of sequences better than 10.0: 104
Number of HSP's better than 10.0 without gapping: 747
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 799
length of query: 422
length of database: 4,897,600
effective HSP length: 93
effective length of query: 329
effective length of database: 2,250,820
effective search space: 740519780
effective search space used: 740519780
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.7 bits)
S2: 56 (26.2 bits)
Medicago: description of AC139841.8