BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139525.1 - phase: 0
(395 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC11362 similar to UP|Q8LGG5 (Q8LGG5) Serine protein kinase-like... 267 2e-72
AU252052 74 6e-14
BP062213 70 8e-13
AV422334 62 1e-10
BG662003 61 3e-10
BE122539 54 6e-08
TC16554 homologue to UP|AAN71719 (AAN71719) Glycogen synthase ki... 49 2e-06
TC11248 homologue to UP|P93323 (P93323) Cdc2MsF protein, partial... 49 2e-06
AW719670 47 7e-06
TC8079 homologue to UP|Q9M6S1 (Q9M6S1) MAP kinase 3, complete 47 7e-06
TC9391 homologue to UP|MSK1_MEDSA (P51137) Glycogen synthase kin... 45 2e-05
TC8547 homologue to UP|MMK1_MEDSA (Q07176) Mitogen-activated pro... 45 2e-05
TC17660 homologue to UP|Q9M6R8 (Q9M6R8) MAP kinase PsMAPK2, part... 44 4e-05
BP065971 43 8e-05
TC9773 homologue to UP|KSGI_ARATH (Q39012) Shaggy-related protei... 43 8e-05
AV416253 43 1e-04
TC15636 similar to UP|Q93WR7 (Q93WR7) MAP kinase kinase, partial... 40 5e-04
TC14602 UP|Q8GS56 (Q8GS56) Phosphoenolpyruvate carboxylase kinas... 40 5e-04
BI418434 39 0.002
AV422094 38 0.003
>TC11362 similar to UP|Q8LGG5 (Q8LGG5) Serine protein kinase-like protein,
partial (38%)
Length = 828
Score = 267 bits (683), Expect = 2e-72
Identities = 127/141 (90%), Positives = 137/141 (97%)
Frame = +2
Query: 248 KEASEGVDGNHETSGAVESSPNASSAREQASSSAGTSRLSDADGMKLKEQGNRRGSRTVR 307
KEASEGV+GN ETSGAV SSPNASSAREQASS+AGTSRLSDADG K+K+QGN+RGSR++R
Sbjct: 2 KEASEGVEGNPETSGAVASSPNASSAREQASSAAGTSRLSDADGTKVKDQGNKRGSRSMR 181
Query: 308 QKLLASADVKCKLVDFGNACWTYKQFTNDIQTRQYRCPEVILGSKYSTSADLWSFACICF 367
QKL+AS D+KCKLVDFGNACWTYKQFTNDIQTRQYRCPEV+LGSKYSTSADLWSFACICF
Sbjct: 182 QKLMASIDLKCKLVDFGNACWTYKQFTNDIQTRQYRCPEVLLGSKYSTSADLWSFACICF 361
Query: 368 ELATGDVLFDPHSGDNFDRDE 388
ELATGDVLFDPHSGDNFDRDE
Sbjct: 362 ELATGDVLFDPHSGDNFDRDE 424
>AU252052
Length = 324
Score = 73.6 bits (179), Expect = 6e-14
Identities = 40/98 (40%), Positives = 64/98 (64%)
Frame = +1
Query: 88 EITILQQIAEGDTDDKKCVVKLLDHFKHSGPNGQHVCMVFEYLGDNLLTLIKYSDYRGMP 147
EI +LQQ+ + D +CV ++ + F + H+C+VFE LG +L ++ ++YR P
Sbjct: 1 EIEMLQQLGKHDKGGSRCV-QIRNWFDYRN----HICIVFEKLGPSLYDFLRKNNYRSFP 165
Query: 148 INMVKEICFHILVGLDYLHKQLSIIHTDLKPENILLLS 185
I++V+EI +L + ++H L +IHTDLKPENILL+S
Sbjct: 166 IDLVREIGRQLLECIAFMH-DLCMIHTDLKPENILLVS 276
>BP062213
Length = 393
Score = 69.7 bits (169), Expect = 8e-13
Identities = 29/48 (60%), Positives = 35/48 (72%)
Frame = +1
Query: 10 TESSDFTSEDEGTEDYRRGGYHAVRIGDTFSSGRYVVQSKLGWGHFST 57
+ SS +DEG E YR+GGYHAVR+ D F+ GRY+ Q KLGWG FST
Sbjct: 220 SSSSGSEEDDEGLESYRKGGYHAVRVADQFAGGRYIAQRKLGWGQFST 363
>AV422334
Length = 493
Score = 62.4 bits (150), Expect = 1e-10
Identities = 26/61 (42%), Positives = 38/61 (61%)
Frame = +1
Query: 319 KLVDFGNACWTYKQFTNDIQTRQYRCPEVILGSKYSTSADLWSFACICFELATGDVLFDP 378
K++DFG+ + ++ + TR YR PEVILG +S+ D+WS CI EL +G+ LF
Sbjct: 199 KVIDFGSTAYEHQGHNYIVSTRHYRAPEVILGLGWSSPCDIWSVGCILMELCSGEALFQT 378
Query: 379 H 379
H
Sbjct: 379 H 381
Score = 35.8 bits (81), Expect = 0.013
Identities = 17/38 (44%), Positives = 28/38 (72%)
Frame = +1
Query: 149 NMVKEICFHILVGLDYLHKQLSIIHTDLKPENILLLST 186
++V+E+ +L + ++H L +IHTDLKPENIL +S+
Sbjct: 1 DLVRELGRQLLECVAFMH-DLRLIHTDLKPENILFISS 111
>BG662003
Length = 448
Score = 61.2 bits (147), Expect = 3e-10
Identities = 36/93 (38%), Positives = 49/93 (51%), Gaps = 2/93 (2%)
Frame = +1
Query: 258 HETSGAVESSPNASSAREQASSSAGTSRLSDADGMKLKEQGNRRGSRTVRQKLLASAD-- 315
HE G+ +SSP + ++ +A + G+ L + + S R+ A A
Sbjct: 148 HEGKGSNDSSPKRDADQKTREKTADLLT-KEVSGISLDKTSSSPSSGEKRKAEDAHAFDI 324
Query: 316 VKCKLVDFGNACWTYKQFTNDIQTRQYRCPEVI 348
+ K+ D GNACWT FTNDIQTRQYR PEVI
Sbjct: 325 ISVKIADLGNACWTNHHFTNDIQTRQYRSPEVI 423
>BE122539
Length = 268
Score = 53.5 bits (127), Expect = 6e-08
Identities = 27/60 (45%), Positives = 33/60 (55%), Gaps = 2/60 (3%)
Frame = +1
Query: 322 DFGNACWTYKQFTND--IQTRQYRCPEVILGSKYSTSADLWSFACICFELATGDVLFDPH 379
+F + TYK+ + TR YR PEVILG +S D WS CI EL TG+ LF H
Sbjct: 1 EFCTSSTTYKREDQSYIVSTRHYRAPEVILGLGWSYPCDTWSVGCILVELCTGEALFQTH 180
>TC16554 homologue to UP|AAN71719 (AAN71719) Glycogen synthase kinase 3 beta
protein kinase DWARF12 , partial (71%)
Length = 927
Score = 48.5 bits (114), Expect = 2e-06
Identities = 29/61 (47%), Positives = 36/61 (58%), Gaps = 3/61 (4%)
Frame = +1
Query: 319 KLVDFGNACWTYKQFTND--IQTRQYRCPEVILGS-KYSTSADLWSFACICFELATGDVL 375
KL DFG+A K N I +R YR PE+I G+ +YSTS D+WS C+ EL G L
Sbjct: 661 KLCDFGSAKMLVKGEANISYICSRFYRAPELIFGATEYSTSIDIWSAGCVLAELLLGQPL 840
Query: 376 F 376
F
Sbjct: 841 F 843
Score = 48.1 bits (113), Expect = 2e-06
Identities = 39/143 (27%), Positives = 65/143 (45%), Gaps = 3/143 (2%)
Frame = +1
Query: 44 YVVQSKLGWGHFSTVWLAWDSHHSRYVALKVQKSAQHYTEAALDEITILQQIAEGDTDDK 103
Y+ + +G G F V+ A VA+K + Y L + ++ D
Sbjct: 238 YMAERVVGTGSFGIVFQAKCLESGEAVAIKKVLQDRRYKNRELQLMRVM---------DH 390
Query: 104 KCVVKLLD-HFKHSGPNGQHVCMVFEYLGDNLLTLIKY--SDYRGMPINMVKEICFHILV 160
VV L F + + + +V EY+ +++ +IK+ + + MPI VK + I
Sbjct: 391 PNVVSLKHCFFSTTSTDELFLNLVMEYVPESMYRVIKHYSNANQRMPIIYVKLYMYQIFR 570
Query: 161 GLDYLHKQLSIIHTDLKPENILL 183
GL Y+H + H DLKP+NIL+
Sbjct: 571 GLAYIHTVPGVCHRDLKPQNILV 639
>TC11248 homologue to UP|P93323 (P93323) Cdc2MsF protein, partial (72%)
Length = 747
Score = 48.5 bits (114), Expect = 2e-06
Identities = 39/141 (27%), Positives = 69/141 (48%), Gaps = 6/141 (4%)
Frame = +1
Query: 49 KLGWGHFSTVWLAWDSHHSRYVALK---VQKSAQHYTEAALDEITILQQIAEGDTDDKKC 105
K+G G + V+ A + + VALK + + + L E++IL+ ++
Sbjct: 64 KVGEGTYGKVYRAREKATGKIVALKKTRLHEDDEGVPPTTLREVSILRMLSRDPH----- 228
Query: 106 VVKLLDHFKHSGPNGQHVC-MVFEYLGDNLLTLIKYSDYRG--MPINMVKEICFHILVGL 162
VV+L+D + G+ V +VFEY+ +L I+ G +P VK + + + G+
Sbjct: 229 VVRLMDVKQGQSKEGKTVLYLVFEYMDTDLKKFIRTFRQTGQNVPPKTVKSLMYQLRKGV 408
Query: 163 DYLHKQLSIIHTDLKPENILL 183
+ H I+H DLKP N+L+
Sbjct: 409 AFCHGH-GILHRDLKPHNLLM 468
Score = 32.0 bits (71), Expect = 0.18
Identities = 16/31 (51%), Positives = 20/31 (63%), Gaps = 1/31 (3%)
Frame = +2
Query: 346 EVILGSK-YSTSADLWSFACICFELATGDVL 375
EV+LG+ YS + D+WS ACI EL T L
Sbjct: 581 EVLLGATHYSMAVDMWSVACIFAELVTKQAL 673
>AW719670
Length = 597
Score = 46.6 bits (109), Expect = 7e-06
Identities = 35/147 (23%), Positives = 72/147 (48%)
Frame = +2
Query: 34 RIGDTFSSGRYVVQSKLGWGHFSTVWLAWDSHHSRYVALKVQKSAQHYTEAALDEITILQ 93
R+G+ F GR K+G G F ++L + + VA+K++ + + + + L
Sbjct: 203 RVGNKFRLGR-----KIGSGSFGEIYLGTNIQTNEEVAVKLENIKTKHPQLLYE--SKLY 361
Query: 94 QIAEGDTDDKKCVVKLLDHFKHSGPNGQHVCMVFEYLGDNLLTLIKYSDYRGMPINMVKE 153
+I +G T + + K G G++ +V + LG +L L + + R + + V
Sbjct: 362 KILQGGTG--------IPNVKWYGVEGEYNVLVMDLLGPSLEDLFNFCN-RKLSLKTVLM 514
Query: 154 ICFHILVGLDYLHKQLSIIHTDLKPEN 180
+ ++ ++Y+H + S +H D+KP+N
Sbjct: 515 LADQMINRVEYIHSK-SFLHRDIKPDN 592
>TC8079 homologue to UP|Q9M6S1 (Q9M6S1) MAP kinase 3, complete
Length = 1550
Score = 46.6 bits (109), Expect = 7e-06
Identities = 27/70 (38%), Positives = 40/70 (56%), Gaps = 3/70 (4%)
Frame = +3
Query: 310 LLASADVKCKLVDFGNACWTYKQ--FTNDIQTRQYRCPEVILGSK-YSTSADLWSFACIC 366
LL +++ K++DFG A T + T + TR YR PE++L S Y+++ D+WS CI
Sbjct: 687 LLLNSNCDLKIIDFGLARPTVENDFMTEYVVTRWYRAPELLLNSSDYTSAIDVWSVGCIF 866
Query: 367 FELATGDVLF 376
EL LF
Sbjct: 867 MELMNKKPLF 896
Score = 35.4 bits (80), Expect = 0.017
Identities = 17/33 (51%), Positives = 22/33 (66%)
Frame = +3
Query: 156 FHILVGLDYLHKQLSIIHTDLKPENILLLSTID 188
+ +L GL Y+H +IIH DLKP N+LL S D
Sbjct: 615 YQVLRGLKYIHSA-NIIHRDLKPSNLLLNSNCD 710
>TC9391 homologue to UP|MSK1_MEDSA (P51137) Glycogen synthase kinase-3
homolog MsK-1 , partial (48%)
Length = 841
Score = 45.1 bits (105), Expect = 2e-05
Identities = 33/142 (23%), Positives = 64/142 (44%), Gaps = 2/142 (1%)
Frame = +1
Query: 44 YVVQSKLGWGHFSTVWLAWDSHHSRYVALKVQKSAQHYTEAALDEITILQQIAEGDTDDK 103
Y+ + +G G F V+ A VA+K + Y L + +L D
Sbjct: 439 YMAERIVGHGSFGVVFQAKCLETGETVAIKKVLQDKRYKNRELQTMRLL--------DHP 594
Query: 104 KCVVKLLDHFKHSGPNGQHVCMVFEYLGDNLLTLIKYSDY--RGMPINMVKEICFHILVG 161
V F + + ++ +V EY+ + + +IK+ + + +P+ VK + I
Sbjct: 595 NVVALKHCFFSTTEKDELYLNLVLEYVPETVHRVIKHYNKLNQRLPLIYVKLYTYQIFRA 774
Query: 162 LDYLHKQLSIIHTDLKPENILL 183
L Y+H+ + + H D+KP+N+L+
Sbjct: 775 LSYIHRCIRVCHRDIKPQNLLV 840
>TC8547 homologue to UP|MMK1_MEDSA (Q07176) Mitogen-activated protein
kinase homolog MMK1 (MAP kinase MSK7) (MAP kinase ERK1)
, partial (71%)
Length = 1098
Score = 45.1 bits (105), Expect = 2e-05
Identities = 28/70 (40%), Positives = 38/70 (54%), Gaps = 3/70 (4%)
Frame = +1
Query: 310 LLASADVKCKLVDFGNACWTYKQ--FTNDIQTRQYRCPEVILGSK-YSTSADLWSFACIC 366
LL +A+ K+ DFG A T + T + TR YR PE++L S Y+ + D+WS CI
Sbjct: 229 LLLNANCDLKICDFGLARVTSETDFMTEYVVTRWYRAPELLLNSSDYTAAIDVWSVGCIF 408
Query: 367 FELATGDVLF 376
EL LF
Sbjct: 409 MELMDRKPLF 438
Score = 35.4 bits (80), Expect = 0.017
Identities = 20/66 (30%), Positives = 37/66 (55%)
Frame = +1
Query: 123 VCMVFEYLGDNLLTLIKYSDYRGMPINMVKEICFHILVGLDYLHKQLSIIHTDLKPENIL 182
V + +E + +L +I+ + +G+ + + IL GL Y+H +++H DLKP N+L
Sbjct: 64 VYIAYELMDTDLHQIIRSN--QGLSEEHCQYFLYQILRGLKYIHSA-NVLHRDLKPSNLL 234
Query: 183 LLSTID 188
L + D
Sbjct: 235 LNANCD 252
>TC17660 homologue to UP|Q9M6R8 (Q9M6R8) MAP kinase PsMAPK2, partial (64%)
Length = 717
Score = 44.3 bits (103), Expect = 4e-05
Identities = 27/64 (42%), Positives = 35/64 (54%), Gaps = 4/64 (6%)
Frame = +2
Query: 310 LLASADVKCKLVDFGNA---CWTYKQFTNDIQTRQYRCPEVILG-SKYSTSADLWSFACI 365
LL +A+ K+ DFG A C + T + TR YR PE++L Y TS D+WS CI
Sbjct: 326 LLINANCDLKICDFGLARINCSKNQFMTEYVVTRWYRAPELLLCCDNYGTSIDVWSVGCI 505
Query: 366 CFEL 369
EL
Sbjct: 506 FAEL 517
Score = 37.7 bits (86), Expect = 0.003
Identities = 23/66 (34%), Positives = 38/66 (56%)
Frame = +2
Query: 123 VCMVFEYLGDNLLTLIKYSDYRGMPINMVKEICFHILVGLDYLHKQLSIIHTDLKPENIL 182
V +V+E + +L +IK S + + + + F +L GL YLH +I+H DLKP N+L
Sbjct: 161 VYLVYELMDTDLHQIIKSS--QSLSNDHCQYFLFQLLRGLKYLHSA-NILHRDLKPGNLL 331
Query: 183 LLSTID 188
+ + D
Sbjct: 332 INANCD 349
>BP065971
Length = 457
Score = 43.1 bits (100), Expect = 8e-05
Identities = 22/52 (42%), Positives = 30/52 (57%)
Frame = +2
Query: 41 SGRYVVQSKLGWGHFSTVWLAWDSHHSRYVALKVQKSAQHYTEAALDEITIL 92
S RY+V+ LG G F V WD + VA+K+ K+ Y + AL E+TIL
Sbjct: 296 SKRYIVKDVLGQGTFGQVAKCWDPESNSXVAVKIIKNQPAYYQQALXEVTIL 451
>TC9773 homologue to UP|KSGI_ARATH (Q39012) Shaggy-related protein kinase
iota (ASK-iota) , partial (47%)
Length = 774
Score = 43.1 bits (100), Expect = 8e-05
Identities = 35/143 (24%), Positives = 64/143 (44%), Gaps = 3/143 (2%)
Frame = +1
Query: 44 YVVQSKLGWGHFSTVWLAWDSHHSRYVALKVQKSAQHYTEAALDEITILQQIAEGDTDDK 103
Y+ + +G G F V+ A VA+K + Y L + ++ D
Sbjct: 313 YMAERVVGTGSFGVVFQAKCLETGEAVAIKKVLQDRRYKNRELQLMRLM---------DH 465
Query: 104 KCVVKLLD-HFKHSGPNGQHVCMVFEYLGDNLLTLIKY--SDYRGMPINMVKEICFHILV 160
V+ L F + + + +V +Y+ + L ++K+ S + MP+ VK + I
Sbjct: 466 PNVISLKHCFFSTTSRDELFLNLVMDYVPETLYRVLKHYNSMNQRMPLIYVKLYTYQIFR 645
Query: 161 GLDYLHKQLSIIHTDLKPENILL 183
GL Y+H + H D+KP+N+L+
Sbjct: 646 GLAYIHTVPGVSHRDVKPQNLLV 714
>AV416253
Length = 408
Score = 42.7 bits (99), Expect = 1e-04
Identities = 17/19 (89%), Positives = 19/19 (99%)
Frame = +1
Query: 370 ATGDVLFDPHSGDNFDRDE 388
ATGDVLFDPHSGDN++RDE
Sbjct: 4 ATGDVLFDPHSGDNYERDE 60
>TC15636 similar to UP|Q93WR7 (Q93WR7) MAP kinase kinase, partial (46%)
Length = 1184
Score = 40.4 bits (93), Expect = 5e-04
Identities = 19/45 (42%), Positives = 26/45 (57%)
Frame = +2
Query: 147 PINMVKEICFHILVGLDYLHKQLSIIHTDLKPENILLLSTIDPSK 191
P + IC +L GL YLH + IIH DLKP N+L+ +P +
Sbjct: 2 PEPYLASICKQVLKGLMYLHHEKHIIHRDLKPSNLLINHRGEPER 136
>TC14602 UP|Q8GS56 (Q8GS56) Phosphoenolpyruvate carboxylase kinase, complete
Length = 1256
Score = 40.4 bits (93), Expect = 5e-04
Identities = 22/67 (32%), Positives = 35/67 (51%), Gaps = 2/67 (2%)
Frame = +2
Query: 319 KLVDFGNACW--TYKQFTNDIQTRQYRCPEVILGSKYSTSADLWSFACICFELATGDVLF 376
KL DFG+A W ++ + + T Y PEV++G +Y D+WS I + + +G
Sbjct: 491 KLADFGSAEWFGDGRRMSGVVGTPYYVAPEVLMGREYGEKVDVWSCGVILYIMLSGT--- 661
Query: 377 DPHSGDN 383
P GD+
Sbjct: 662 PPFYGDS 682
>BI418434
Length = 478
Score = 38.9 bits (89), Expect = 0.002
Identities = 21/64 (32%), Positives = 36/64 (55%), Gaps = 2/64 (3%)
Frame = +2
Query: 122 HVCMVFEYLGDNLLTLIKYSDYRGMPINMVKEICFHILVGLDYLHKQLS--IIHTDLKPE 179
H +V+EY+ + L+ D + ++ ++ G+ YLH++L I+H D+KPE
Sbjct: 218 HRLLVYEYMHNGALSAYLRKDGPCLSWDVRFQVAIGTAKGIAYLHEELRCCILHCDIKPE 397
Query: 180 NILL 183
NILL
Sbjct: 398 NILL 409
>AV422094
Length = 462
Score = 38.1 bits (87), Expect = 0.003
Identities = 22/66 (33%), Positives = 34/66 (51%), Gaps = 2/66 (3%)
Frame = +2
Query: 120 GQHVCMVFEYLGDNLLTLIKYSDYRGMPINMVKEICFHILVGLDYLHKQ--LSIIHTDLK 177
G +V+EYL + L + + + +IC + GL YLH++ L I+H D+K
Sbjct: 248 GSKRLLVYEYLENKSLDQALFGKALSLNWSTRYDICLGVARGLTYLHEESRLRIVHRDVK 427
Query: 178 PENILL 183
NILL
Sbjct: 428 ASNILL 445
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.316 0.133 0.395
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,887,539
Number of Sequences: 28460
Number of extensions: 95233
Number of successful extensions: 698
Number of sequences better than 10.0: 114
Number of HSP's better than 10.0 without gapping: 672
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 690
length of query: 395
length of database: 4,897,600
effective HSP length: 92
effective length of query: 303
effective length of database: 2,279,280
effective search space: 690621840
effective search space used: 690621840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 56 (26.2 bits)
Medicago: description of AC139525.1