BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC139343.2 + phase: 0 /pseudo
(443 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC9039 60 9e-10
TC8302 32 0.16
TC8301 32 0.27
BP065763 31 0.47
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 30 1.0
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 30 1.0
BP054557 29 1.4
AV769820 29 1.8
TC18421 similar to UP|Q9ZV23 (Q9ZV23) At2g28910 protein (At2g289... 29 1.8
TC14520 similar to UP|Q8W194 (Q8W194) Zinc finger protein LSD2, ... 28 2.3
TC12447 weakly similar to GB|AAM63773.1|21555085|AY086719 phytoc... 28 3.0
TC11916 similar to UP|P94078 (P94078) Alpha-mannosidase (AT3G267... 28 4.0
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 28 4.0
AV408988 28 4.0
AV773553 28 4.0
AV421607 27 5.2
BI418821 27 6.8
TC17929 27 6.8
AW164042 27 6.8
>TC9039
Length = 1218
Score = 59.7 bits (143), Expect = 9e-10
Identities = 35/85 (41%), Positives = 46/85 (53%)
Frame = +3
Query: 349 SYISVGDDEDQVYLGDSRTTAVNGKGKVILKLTSGKTLALSDVLNVPTIRTNLISVALLN 408
SY + D E +Y+GD +T V G L L +G L L D VP+ R NLISV+ L+
Sbjct: 927 SYRKLNDVERYIYVGDGKTVEVEAIGHFRLLLCTGFYLDLKDTFVVPSFRRNLISVSNLD 1106
Query: 409 KVGVKVSFESDKMVMSKNNVFVGNG 433
K G SF + K +S N+ VG G
Sbjct: 1107 KSGYSCSFGNSKAELSFNSNIVGTG 1181
Score = 45.4 bits (106), Expect(2) = 5e-06
Identities = 44/232 (18%), Positives = 89/232 (37%), Gaps = 5/232 (2%)
Frame = +2
Query: 72 VKPDEAKSDAKQIEKWNNANKVCRHTILSTISDALFDVYCS-YKVAKDIWDNMNVKYTAE 130
++ K EKW+ +N++ I I + K AKD + ++
Sbjct: 260 LRKSSTSEQRKDYEKWDRSNRMSLMIIKRGIPEVFRGTISEEIKGAKDFLAEIEKRFAKS 439
Query: 131 EATKQKFVVGNYL*WQMKEDKEIKVQINEYHQLIEELKAENIILPDVFVAAALVEKLRSS 190
+ + ++ N + + + I+ I + +LKA + L D + ++ L +
Sbjct: 440 DKAETSTLLQNLISMKYQGKGNIREYIMGMSNIASKLKALKLELSDDLLIHLVLLSLPAQ 619
Query: 191 WDDYKQQLKHKHKQMSLNDLIRHIIIEDTSRKECGAARAKALESRANLIQNNARKQQRYE 250
+ +K ++ SLN+LI + E+ K+ K + ++ ++++ E
Sbjct: 620 FSQFKISYNCPKEKWSLNELISFCVQEEERLKQ----ERKESAHFVSTSKDKGKRKKTVE 787
Query: 251 NKSDHALKVTNPNFKANLGECYVCGKKGHKAYHC----RYRKVNGQPPKPKA 298
K++ A P + CY C GH C +R G P P+A
Sbjct: 788 PKNE-AADAPAPKKQKEDDTCYFCNVSGHMKKKCTKYHAWRARKGLPKLPEA 940
Score = 21.2 bits (43), Expect(2) = 5e-06
Identities = 9/38 (23%), Positives = 18/38 (46%)
Frame = +1
Query: 37 DVSRIEVFKGQNFRRWNERVFTLLDVHGVASVLTDVKP 74
+++ + + G NF+ W E + +L + L KP
Sbjct: 139 NLNSVPILDGTNFKDWKENMEIVLGCMDLDLALRVEKP 252
>TC8302
Length = 494
Score = 32.3 bits (72), Expect = 0.16
Identities = 36/147 (24%), Positives = 60/147 (40%), Gaps = 4/147 (2%)
Frame = +3
Query: 146 QMKEDKEIKVQINEYHQLIEELKAENIILPDVFVAAALVEKLRSSWDDYKQQLKHKH--K 203
+M E + I+ + L +L A N + + A L+E L S+D +K+ +
Sbjct: 21 RMSESTSMSDHIDHMNTLFAQLSASNFTIGENERAELLLESLPDSYDQLVINIKNNNIVN 200
Query: 204 QMSLNDLIRHIIIEDTSR--KECGAARAKALESRANLIQNNARKQQRYENKSDHALKVTN 261
+ L L ++ ED+ R KE +K +E+ L R + R
Sbjct: 201 HLPLMMLSEVLLEEDSQR*NKEDR*DSSKPMEA---LTMTRGRSKSRK------------ 335
Query: 262 PNFKANLGECYVCGKKGHKAYHCRYRK 288
K NL +CY CG++ H C + K
Sbjct: 336 ---KINL-KCYHCGQR*HLKKDCWFNK 404
>TC8301
Length = 1243
Score = 31.6 bits (70), Expect = 0.27
Identities = 19/83 (22%), Positives = 33/83 (38%)
Frame = +2
Query: 41 IEVFKGQNFRRWNERVFTLLDVHGVASVLTDVKPDEAKSDAKQIEKWNNANKVCRHTILS 100
I F G NF W ++ +L + D +P + D KW + +
Sbjct: 518 IPKFSGSNFSLWKLKIKAILRKDNCLPAI-DGRPADITDD-----KWKEMDDNAVANLHL 679
Query: 101 TISDALFDVYCSYKVAKDIWDNM 123
++D++ K AK+IWD +
Sbjct: 680 AVADSVLSSIAEKKTAKEIWDTL 748
>BP065763
Length = 537
Score = 30.8 bits (68), Expect = 0.47
Identities = 19/62 (30%), Positives = 29/62 (46%)
Frame = -2
Query: 342 AKREAFTSYISVGDDEDQVYLGDSRTTAVNGKGKVILKLTSGKTLALSDVLNVPTIRTNL 401
+KR+ F S + E+ +Y+G+ + G G L L +G L L L V NL
Sbjct: 320 SKRKGFLSIHPIRGTENFLYMGNQMKARIEGIGTYRLILDTGHQLDLEKCLYVRGCARNL 141
Query: 402 IS 403
+S
Sbjct: 140 VS 135
Score = 30.8 bits (68), Expect = 0.47
Identities = 18/44 (40%), Positives = 19/44 (42%)
Frame = -1
Query: 245 KQQRYENKSDHALKVTNPNFKANLGECYVCGKKGHKAYHCRYRK 288
K R E K D ALKV CY C K GH +C RK
Sbjct: 456 KPGRKEQKKDQALKVKEGRIHKE-HVCYFCKKAGHFKKYCPKRK 328
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 29.6 bits (65), Expect = 1.0
Identities = 14/39 (35%), Positives = 22/39 (55%), Gaps = 5/39 (12%)
Frame = +1
Query: 270 ECYVCGKKGHKAYHCRYRKVNGQ-----PPKPKANLTQG 303
+CY CG+ GH A CR R +G+ PP+ + + + G
Sbjct: 376 KCYECGEPGHFARECRMRGGSGRRRSRSPPRFRRSPSYG 492
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 29.6 bits (65), Expect = 1.0
Identities = 11/19 (57%), Positives = 13/19 (67%)
Frame = +2
Query: 266 ANLGECYVCGKKGHKAYHC 284
+N GEC+ CGK GH A C
Sbjct: 404 SNGGECFKCGKPGHFAREC 460
>BP054557
Length = 538
Score = 29.3 bits (64), Expect = 1.4
Identities = 25/82 (30%), Positives = 39/82 (47%), Gaps = 3/82 (3%)
Frame = -2
Query: 331 VVDSGATRHICAKREAFTSYISVGDDEDQVYLGDSRTTAVNGKGKVILKLTS---GKTLA 387
++D+GAT H+C + F + V V L + NG+ ++ S +
Sbjct: 537 LLDTGATHHVCHNLDVFQALRQV----HPVLL-----SMPNGQKEIAHMADSVYFSEKFF 385
Query: 388 LSDVLNVPTIRTNLISVALLNK 409
L+ VL VP+ NLISV+ L K
Sbjct: 384 LAXVLYVPSFNFNLISVSALVK 319
>AV769820
Length = 316
Score = 28.9 bits (63), Expect = 1.8
Identities = 13/28 (46%), Positives = 18/28 (63%)
Frame = -2
Query: 271 CYVCGKKGHKAYHCRYRKVNGQPPKPKA 298
C VCGKKGH + +C +R+ Q + KA
Sbjct: 261 CNVCGKKGHLSIYC-WRRCEKQANERKA 181
>TC18421 similar to UP|Q9ZV23 (Q9ZV23) At2g28910 protein
(At2g28910/F8N16.20), partial (28%)
Length = 553
Score = 28.9 bits (63), Expect = 1.8
Identities = 9/28 (32%), Positives = 16/28 (57%)
Frame = +2
Query: 258 KVTNPNFKANLGECYVCGKKGHKAYHCR 285
++TN + + G C CG+ GH + C+
Sbjct: 392 RITNTDVDVSRGACKKCGRVGHLKFQCK 475
>TC14520 similar to UP|Q8W194 (Q8W194) Zinc finger protein LSD2, partial
(84%)
Length = 1112
Score = 28.5 bits (62), Expect = 2.3
Identities = 17/59 (28%), Positives = 26/59 (43%)
Frame = +2
Query: 335 GATRHICAKREAFTSYISVGDDEDQVYLGDSRTTAVNGKGKVILKLTSGKTLALSDVLN 393
GAT CA T+ G D Q+Y G RT + +G ++ + T+ L+ N
Sbjct: 332 GATNVCCALCNTITAVPPPGMDMSQLYCGGCRTLLMYTRGAASVRCSCCHTVNLAPAAN 508
>TC12447 weakly similar to GB|AAM63773.1|21555085|AY086719
phytocyanin-related protein-like {Arabidopsis thaliana;}
, partial (56%)
Length = 735
Score = 28.1 bits (61), Expect = 3.0
Identities = 15/48 (31%), Positives = 27/48 (56%)
Frame = +3
Query: 112 SYKVAKDIWDNMNVKYTAEEATKQKFVVGNYL*WQMKEDKEIKVQINE 159
++KV D++N + A K +F VG+YL W+ K+ +Q+N+
Sbjct: 141 AWKVPSSEADSLN-----QWAEKSRFKVGDYLVWKYDGGKDSVLQVNK 269
>TC11916 similar to UP|P94078 (P94078) Alpha-mannosidase
(AT3G26720/MLJ15_12), partial (14%)
Length = 547
Score = 27.7 bits (60), Expect = 4.0
Identities = 10/23 (43%), Positives = 16/23 (69%)
Frame = +3
Query: 340 ICAKREAFTSYISVGDDEDQVYL 362
+CA+R F ++++G E QVYL
Sbjct: 255 MCAERAGFCDFLALGGHESQVYL 323
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 27.7 bits (60), Expect = 4.0
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +1
Query: 270 ECYVCGKKGHKAYHCRYR 287
+CY CG+ GH A CR R
Sbjct: 163 KCYECGEPGHFARECRSR 216
>AV408988
Length = 365
Score = 27.7 bits (60), Expect = 4.0
Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 2/34 (5%)
Frame = +1
Query: 277 KGHKAYHCRYRKVNGQPP--KPKANLTQGDDKKY 308
+G ++YH Y+ G PP + K NL D KY
Sbjct: 115 EGERSYHIFYQLCAGAPPSLREKLNLQSAQDYKY 216
>AV773553
Length = 344
Score = 27.7 bits (60), Expect = 4.0
Identities = 11/16 (68%), Positives = 11/16 (68%)
Frame = +3
Query: 297 KANLTQGDDKKYDDDD 312
K N GDD KYDDDD
Sbjct: 297 KENEADGDDDKYDDDD 344
>AV421607
Length = 245
Score = 27.3 bits (59), Expect = 5.2
Identities = 12/32 (37%), Positives = 14/32 (43%), Gaps = 1/32 (3%)
Frame = +3
Query: 271 CYVCGKKGHKAYHCRYRKVNGQP-PKPKANLT 301
CY CG+ GH + C N P P P T
Sbjct: 54 CYKCGRPGHWSRDCPSSAPNSNPNPNPNTTTT 149
>BI418821
Length = 614
Score = 26.9 bits (58), Expect = 6.8
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +2
Query: 269 GECYVCGKKGHKAYHCRYRKVNG 291
G CY CG GH A C NG
Sbjct: 386 GGCYNCGDTGHLARDCHRSNNNG 454
>TC17929
Length = 791
Score = 26.9 bits (58), Expect = 6.8
Identities = 11/31 (35%), Positives = 15/31 (47%)
Frame = +2
Query: 262 PNFKANLGECYVCGKKGHKAYHCRYRKVNGQ 292
PN CY CG+ GHK +C +G+
Sbjct: 35 PNDSKFRQTCYRCGESGHKMRNCPKEHSSGE 127
>AW164042
Length = 404
Score = 26.9 bits (58), Expect = 6.8
Identities = 11/24 (45%), Positives = 14/24 (57%)
Frame = -2
Query: 91 NKVCRHTILSTISDALFDVYCSYK 114
+KVC H + LFD+ CSYK
Sbjct: 127 SKVCHHCF*YPLHAVLFDLTCSYK 56
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.132 0.384
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,665,304
Number of Sequences: 28460
Number of extensions: 78416
Number of successful extensions: 399
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 394
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 398
length of query: 443
length of database: 4,897,600
effective HSP length: 93
effective length of query: 350
effective length of database: 2,250,820
effective search space: 787787000
effective search space used: 787787000
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 57 (26.6 bits)
Medicago: description of AC139343.2