BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138580.5 + phase: 0 /pseudo
(448 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV767121 133 7e-32
AV780221 120 5e-28
AV780705 62 3e-10
TC13572 53 8e-10
BP060613 49 1e-06
BP078534 40 6e-04
TC12829 37 0.009
BP041113 33 0.12
TC16035 similar to UP|O04919 (O04919) Lipoxygenase , partial (32%) 30 1.1
AV410239 30 1.1
TC12790 similar to PIR|H96696|H96696 protein F1N21.16 [imported]... 29 1.4
TC11422 UP|NIA_LOTJA (P39869) Nitrate reductase [NADH] (NR) , c... 25 1.4
TC19130 similar to UP|Q7YSZ8 (Q7YSZ8) GE-rich protein precursor,... 28 2.4
TC12500 similar to GB|AAH45354.1|28279536|BC045354 FNBP4 protein... 24 2.7
BI418643 28 3.1
TC16618 28 4.0
AV419106 27 6.8
TC11796 similar to GB|AAP12870.1|30017273|BT006221 At4g35840 {Ar... 27 6.8
TC17332 similar to UP|MTD_FRAAN (Q9ZRF1) Probable mannitol dehyd... 27 8.9
BP079877 27 8.9
>AV767121
Length = 525
Score = 133 bits (334), Expect = 7e-32
Identities = 75/188 (39%), Positives = 106/188 (55%), Gaps = 3/188 (1%)
Frame = +2
Query: 182 WLAKSVPFFR--ILLKSVLTALPVYALSFFKAPSGIISSIKSLLNKFFWGEGEDKRKIS* 239
W K+V F L++SVLTALP++ LSFFK P G+ S L+ F WG E++ KI+*
Sbjct: 11 WKQKTVSFGGRIFLIQSVLTALPLFFLSFFKLPIGVGKSCVRLMRNFLWGGSENENKIA* 190
Query: 240 IRWDTLSLRKEYGGLGVTRLREFNVALLGKWCWRLLLDREALWRKVLVARYGVADGGLED 299
++W + KE GGLG+ L FN ALLGK WR L + ++LWR+V+ A+
Sbjct: 191 VKWTDVCKPKELGGLGIKDLFTFNKALLGK*RWRYLTEPDSLWRRVIEAQ---------- 340
Query: 300 GDLEDGGRSCSSWWREIVRIRDGIC-EGGEGWFGACVRRRVGDGAKIDFWRDRWCGDVPL 358
D SSWW +I+ +C E +GWF + +++ VG+G + FW + W G L
Sbjct: 341 ---PDHYSCGSSWWNDIL----SLCPEDADGWFSSGLKKLVGEGDQTKFWSEDWLGSGLL 499
Query: 359 CDRFSRLY 366
RF RLY
Sbjct: 500 SARFRRLY 523
>AV780221
Length = 461
Score = 120 bits (301), Expect = 5e-28
Identities = 65/165 (39%), Positives = 96/165 (57%), Gaps = 3/165 (1%)
Frame = +3
Query: 193 LLKSVLTALPVYALSFFKAPSG-IISSIKSLLNKFFWGEGEDKRKIS*IRWDTLSLRKEY 251
L++SVL ALP++ L FFK P+G +++ + + FF E E +KI+ ++W T+ K+
Sbjct: 9 LIRSVLIALPLFHLPFFKLPTGGFYTNVNN**DPFFGREVEGGKKIAWVKWSTVCRPKDE 188
Query: 252 GGLGVTRLREFNVALLGKWCWRLLLDREALWRKVLVARYGVADGGLEDGDLEDGGRSCSS 311
GGLGV L FN ALLGKW WR+L +R+ LW KVL+ +Y A +S SS
Sbjct: 189 GGLGVQNLGLFNKALLGKWRWRMLKERDGLWYKVLLIKYKNAI-----------PQSASS 335
Query: 312 WWREIVRIRDGIC--EGGEGWFGACVRRRVGDGAKIDFWRDRWCG 354
WW ++ C +GG GW + RR+G+G ++ FW + W G
Sbjct: 336 WWNDL----HSTCFEDGGGGWMQRGLCRRIGEGTEVKFWNENWLG 458
>AV780705
Length = 524
Score = 61.6 bits (148), Expect = 3e-10
Identities = 33/100 (33%), Positives = 57/100 (57%), Gaps = 1/100 (1%)
Frame = -2
Query: 192 ILLKSVLTALPVYALSFFKAPSGIISSIKSLLNKFFW-GEGEDKRKIS*IRWDTLSLRKE 250
+L+K+++ A+P YA++ P +S+ +L+ F+W +GE + + L KE
Sbjct: 361 VLIKAIIQAIPSYAMTIVHFPKTFCNSLYALVADFWWKSQGEGAVEYTGAVGTK*PLSKE 182
Query: 251 YGGLGVTRLREFNVALLGKWCWRLLLDREALWRKVLVARY 290
GG+G LR N+A L + WR+L + EALW +V+ + Y
Sbjct: 181 KGGVGFRDLRTQNLAFLARQAWRVLTNPEALWVRVMKSLY 62
>TC13572
Length = 534
Score = 53.1 bits (126), Expect(2) = 8e-10
Identities = 40/137 (29%), Positives = 63/137 (45%), Gaps = 1/137 (0%)
Frame = -3
Query: 282 WRKVLVARYGVADGGLEDGDLEDGGRSCSSWWREIVRIRDGICEGGE-GWFGACVRRRVG 340
W +V++A+YG DGG + SSWWR++ GGE GWF V + +
Sbjct: 478 WCRVVLAKYG--DGGCTN---------ISSWWRDVQT-----AGGGENGWFEEGVHKVLR 347
Query: 341 DGAKIDFWRDRWCGDVPLCDRFSRLYDLAVNKSITVRNMFLLGVDVGGEALRWRRRLWAW 400
G FW + W G L +++ RLY+L+ K ++ V RWRR L
Sbjct: 346 SGNHTRFWLENWTGVGILREKYYRLYNLSKLKWASIDECGGWSQGVWNWDFRWRRPLAGR 167
Query: 401 EEELVEECRVVLLTVSL 417
E + ++ + ++ V L
Sbjct: 166 ELDWLQALSLDIVRVPL 116
Score = 26.6 bits (57), Expect(2) = 8e-10
Identities = 10/12 (83%), Positives = 10/12 (83%)
Frame = -1
Query: 263 NVALLGKWCWRL 274
N ALLGKW WRL
Sbjct: 534 NKALLGKWLWRL 499
>BP060613
Length = 378
Score = 49.3 bits (116), Expect = 1e-06
Identities = 32/107 (29%), Positives = 52/107 (47%), Gaps = 1/107 (0%)
Frame = +1
Query: 228 WGEGEDKRKIS*IRWDTLSLRKEYGGLGVTRLREFNVALLGKWCWRLLLDREALWRKVLV 287
W + R + +WD L+ K+ GGLG N ALL K WR+L + +ALW ++L
Sbjct: 46 WWSSKGTRGVHWRKWDLLTELKDEGGLGFKDFEIQNQALLAKQAWRILHNPDALWVQILK 225
Query: 288 ARYGVADGGLEDGDLEDGGRSCSSW-WREIVRIRDGICEGGEGWFGA 333
A Y L+ R+ +SW W ++ R+ + + G+ G+
Sbjct: 226 ALYFP-----HHDFLQTTKRTGASWVWSSLLHGRELLLKQGQWQLGS 351
>BP078534
Length = 410
Score = 40.4 bits (93), Expect = 6e-04
Identities = 23/64 (35%), Positives = 32/64 (49%), Gaps = 3/64 (4%)
Frame = -3
Query: 344 KIDFWRDRWCGDVPLCDRFSRLYDLAVNKSITVRNMFLLGVDVGGE---ALRWRRRLWAW 400
+ + W D W + LCD F RL+ L NK V + +G GG L +RR + W
Sbjct: 228 QFNLWLDPWIRERALCDMFPRLFTLCENKGALVAD---VGSYHGGSWHWNLSFRREFFQW 58
Query: 401 EEEL 404
EEE+
Sbjct: 57 EEEI 46
>TC12829
Length = 448
Score = 36.6 bits (83), Expect = 0.009
Identities = 15/36 (41%), Positives = 23/36 (63%)
Frame = +3
Query: 192 ILLKSVLTALPVYALSFFKAPSGIISSIKSLLNKFF 227
+L+KSV A+P Y +S F P I S I+ ++ KF+
Sbjct: 282 VLIKSVTKAIPTYIMSCFALPDSICSQIEGMVGKFY 389
>BP041113
Length = 503
Score = 32.7 bits (73), Expect = 0.12
Identities = 16/55 (29%), Positives = 26/55 (47%)
Frame = +1
Query: 347 FWRDRWCGDVPLCDRFSRLYDLAVNKSITVRNMFLLGVDVGGEALRWRRRLWAWE 401
FW + W G L DR+ RL++L+ K + ++ GG W +W W+
Sbjct: 1 FWTEDWIGMGTLRDRYYRLFNLSKEKRCCI-------LECGG----WNHGVWTWK 132
>TC16035 similar to UP|O04919 (O04919) Lipoxygenase , partial (32%)
Length = 1035
Score = 29.6 bits (65), Expect = 1.1
Identities = 19/68 (27%), Positives = 30/68 (43%), Gaps = 1/68 (1%)
Frame = +3
Query: 282 WRKVLVARYGVADGGL-EDGDLEDGGRSCSSWWREIVRIRDGICEGGEGWFGACVRRRVG 340
W + V Y V+D + ED +L+ SWW+E+V + G +G W R+ +
Sbjct: 177 WVQDYVTLYYVSDDAVKEDSELQ-------SWWKEVVEVGHGDKKGEPWWPKMQTRKDLI 335
Query: 341 DGAKIDFW 348
I W
Sbjct: 336 QTCTIVIW 359
>AV410239
Length = 432
Score = 29.6 bits (65), Expect = 1.1
Identities = 13/25 (52%), Positives = 15/25 (60%)
Frame = -3
Query: 336 RRRVGDGAKIDFWRDRWCGDVPLCD 360
RRR G G + R+RWCG VP D
Sbjct: 232 RRREGVGVASEANRERWCGGVPDAD 158
>TC12790 similar to PIR|H96696|H96696 protein F1N21.16 [imported] -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(29%)
Length = 727
Score = 29.3 bits (64), Expect = 1.4
Identities = 22/59 (37%), Positives = 27/59 (45%)
Frame = +3
Query: 296 GLEDGDLEDGGRSCSSWWREIVRIRDGICEGGEGWFGACVRRRVGDGAKIDFWRDRWCG 354
GL +G+L G S + V +DG+C GG RVG G WR RWCG
Sbjct: 336 GLREGELLLAGVSGAGLE---VAAQDGVCAGG----------RVG*GR----WR*RWCG 461
>TC11422 UP|NIA_LOTJA (P39869) Nitrate reductase [NADH] (NR) , complete
Length = 2932
Score = 25.4 bits (54), Expect(2) = 1.4
Identities = 15/43 (34%), Positives = 21/43 (47%), Gaps = 3/43 (6%)
Frame = +3
Query: 305 GGRSCSSWWREIV--RIRDGICEGGEGWFGACVR-RRVGDGAK 344
GGR S WWR V ++G C+G C+ R G G++
Sbjct: 684 GGRGSSRWWRFQVLDEHQEGGCDGSVA*CYTCLHAERGGSGSR 812
Score = 21.9 bits (45), Expect(2) = 1.4
Identities = 7/18 (38%), Positives = 12/18 (65%)
Frame = +1
Query: 386 VGGEALRWRRRLWAWEEE 403
+GG ++W +R+ EEE
Sbjct: 850 IGGRMVKWLKRIVVTEEE 903
>TC19130 similar to UP|Q7YSZ8 (Q7YSZ8) GE-rich protein precursor, partial
(20%)
Length = 603
Score = 28.5 bits (62), Expect = 2.4
Identities = 13/22 (59%), Positives = 15/22 (68%)
Frame = -2
Query: 321 DGICEGGEGWFGACVRRRVGDG 342
D +GGEG GA V+RR GDG
Sbjct: 125 DAQLKGGEGVTGAVVKRREGDG 60
>TC12500 similar to GB|AAH45354.1|28279536|BC045354 FNBP4 protein {Danio
rerio;} , partial (5%)
Length = 678
Score = 23.9 bits (50), Expect(2) = 2.7
Identities = 12/34 (35%), Positives = 13/34 (37%), Gaps = 4/34 (11%)
Frame = -1
Query: 323 ICEGGEGWFGACVR----RRVGDGAKIDFWRDRW 352
+C G W C R RR G WR RW
Sbjct: 423 VC*GRREWSSICCRHE*RRRRRYGCNAILWRRRW 322
Score = 22.7 bits (47), Expect(2) = 2.7
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = -1
Query: 392 RWRRRLWAW 400
RWRR LW W
Sbjct: 219 RWRRGLWWW 193
>BI418643
Length = 450
Score = 28.1 bits (61), Expect = 3.1
Identities = 10/22 (45%), Positives = 15/22 (67%)
Frame = +1
Query: 152 VSLFGALDWG*SALIIVLGTCC 173
V LFG WG*S+++ ++ CC
Sbjct: 382 VLLFGVALWG*SSVVSIIAACC 447
>TC16618
Length = 1228
Score = 27.7 bits (60), Expect = 4.0
Identities = 14/34 (41%), Positives = 19/34 (55%)
Frame = -1
Query: 295 GGLEDGDLEDGGRSCSSWWREIVRIRDGICEGGE 328
G L G++E GG+ W R VR+R G+C E
Sbjct: 646 GDLSFGEIEGGGKRKRVWVR--VRVRVGVCNERE 551
>AV419106
Length = 192
Score = 26.9 bits (58), Expect = 6.8
Identities = 14/28 (50%), Positives = 15/28 (53%), Gaps = 2/28 (7%)
Frame = -2
Query: 327 GEGWFGACVRRRVGDGAKIDFW--RDRW 352
G GWFG CV V A + FW R RW
Sbjct: 167 GPGWFGDCVWVCVFS*AFLGFW*LRRRW 84
>TC11796 similar to GB|AAP12870.1|30017273|BT006221 At4g35840 {Arabidopsis
thaliana;}, partial (86%)
Length = 1114
Score = 26.9 bits (58), Expect = 6.8
Identities = 12/34 (35%), Positives = 17/34 (49%), Gaps = 1/34 (2%)
Frame = -2
Query: 376 VRNMFLLGVDVG-GEALRWRRRLWAWEEELVEEC 408
+ N+F L ++ G RW RR W WE+ C
Sbjct: 126 ITNLFNLVNEISEGTEQRWTRRAWIWEKIHERNC 25
>TC17332 similar to UP|MTD_FRAAN (Q9ZRF1) Probable mannitol dehydrogenase
(NAD-dependent mannitol dehydrogenase) , partial (63%)
Length = 736
Score = 26.6 bits (57), Expect = 8.9
Identities = 8/13 (61%), Positives = 9/13 (68%)
Frame = +3
Query: 342 GAKIDFWRDRWCG 354
GAK+ WR WCG
Sbjct: 291 GAKVQSWRQSWCG 329
>BP079877
Length = 381
Score = 26.6 bits (57), Expect = 8.9
Identities = 15/37 (40%), Positives = 23/37 (61%)
Frame = -2
Query: 190 FRILLKSVLTALPVYALSFFKAPSGIISSIKSLLNKF 226
FRI ++ + +P+Y LSFF S +ISS+ S + F
Sbjct: 119 FRICIR--VKTIPLYFLSFFLLISFVISSLFSFKSHF 15
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.353 0.160 0.608
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 9,057,346
Number of Sequences: 28460
Number of extensions: 144471
Number of successful extensions: 2335
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 2279
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2325
length of query: 448
length of database: 4,897,600
effective HSP length: 93
effective length of query: 355
effective length of database: 2,250,820
effective search space: 799041100
effective search space used: 799041100
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 37 (21.5 bits)
S2: 57 (26.6 bits)
Medicago: description of AC138580.5