Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC138464.6 + phase: 0 
         (250 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC15755 similar to UP|AAP86661 (AAP86661) 26S proteasome subunit...    33  0.046
TC8654 similar to UP|AAP86661 (AAP86661) 26S proteasome subunit ...    31  0.18
TC12487 similar to UP|Q9SAL0 (Q9SAL0) T8K14.20 protein (At1g7938...    31  0.23
TC10170 similar to UP|PSD3_DAUCA (Q06364) Probable 26S proteasom...    30  0.39
AV777154                                                               29  0.87
BP071543                                                               27  4.3
TC8174 similar to GB|AAO24559.1|27808558|BT003127 At5g39890 {Ara...    27  4.3

>TC15755 similar to UP|AAP86661 (AAP86661) 26S proteasome subunit RPN6a,
           partial (30%)
          Length = 540

 Score = 33.1 bits (74), Expect = 0.046
 Identities = 23/101 (22%), Positives = 47/101 (45%), Gaps = 4/101 (3%)
 Frame = +2

Query: 109 KQVVSSMYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQKD 168
           + +  ++ ++N+ RL + +  + +  IA  + L   +  E  + QMI D +   T++Q  
Sbjct: 80  QSLYGTLMEQNLCRLIEPFSRVEIAHIAELIEL-PIEHVERKLSQMILDKKFAGTLDQGA 256

Query: 169 GMVRFLEDPE----QYKTCEMIEHIDSSIQRIMALSKKLTA 205
           G +   +DP+       T E I +I   +  +   S K+ A
Sbjct: 257 GCLIIFDDPKTDAIYPATLETISNIGKVVDSLYVRSAKIMA 379


>TC8654 similar to UP|AAP86661 (AAP86661) 26S proteasome subunit RPN6a,
           partial (31%)
          Length = 615

 Score = 31.2 bits (69), Expect = 0.18
 Identities = 22/101 (21%), Positives = 46/101 (44%), Gaps = 4/101 (3%)
 Frame = +3

Query: 109 KQVVSSMYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQKD 168
           + +  ++ ++N+ RL + +  + +  IA  + L      E  + QMI D +   T++Q  
Sbjct: 93  QSLYGTLMEQNLCRLIEPFSRVEIAHIAELIEL-PIDHVERKLSQMILDKKFAGTLDQGA 269

Query: 169 GMVRFLEDPE----QYKTCEMIEHIDSSIQRIMALSKKLTA 205
           G +   +DP+       T E I +I   +  +   S ++ A
Sbjct: 270 GCLIIFDDPQTDAIYPATLETISNIGKVVDSLYVRSAQIMA 392


>TC12487 similar to UP|Q9SAL0 (Q9SAL0) T8K14.20 protein
           (At1g79380/T8K14_20), partial (57%)
          Length = 1091

 Score = 30.8 bits (68), Expect = 0.23
 Identities = 22/94 (23%), Positives = 46/94 (48%)
 Frame = +2

Query: 99  FEADNNLGLAKQVVSSMYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDG 158
           F +D++     Q V + Y++ +  L  +  T     I   + +      + HVL +I DG
Sbjct: 17  FHSDHSPCHGFQEVLACYQKIVPNLKLSGPTSYAPVIEAAIDIVEKSRGQFHVLVVIADG 196

Query: 159 EIYATINQKDGMVRFLEDPEQYKTCEMIEHIDSS 192
           ++  +++ +DG +     P++ KT + I  +D+S
Sbjct: 197 QVTRSVDTEDGEL----SPQEEKTIKAI--VDAS 280


>TC10170 similar to UP|PSD3_DAUCA (Q06364) Probable 26S proteasome non-ATPase
            regulatory subunit 3 (26S proteasome subunit S3) (Nuclear
            antigen 21D7), partial (79%)
          Length = 1197

 Score = 30.0 bits (66), Expect = 0.39
 Identities = 18/73 (24%), Positives = 32/73 (43%)
 Frame = +3

Query: 72   PCIELAQTYNNGKVAEVEAFVNANAGRFEADNNLGLAKQVVSSMYKRNIQRLTQTYLTLS 131
            P  EL      G +       +  A  F AD    L  ++   + +  ++ ++ +Y  +S
Sbjct: 933  PYFELTNAVRIGDLELFRNIADKFATTFNADGTHNLIVRLRHIVIRTGLRNISISYSRIS 1112

Query: 132  LEDIANTVHLNSA 144
            L D+A  + LNSA
Sbjct: 1113 LADVARKLRLNSA 1151


>AV777154 
          Length = 373

 Score = 28.9 bits (63), Expect = 0.87
 Identities = 14/45 (31%), Positives = 27/45 (59%), Gaps = 4/45 (8%)
 Frame = +2

Query: 53 LPKYASPLAQRNLKLFCQN--PCIELAQTYNNGKVA--EVEAFVN 93
          LP+Y++PL++RN+   C N   C + A   ++ K+   ++  F+N
Sbjct: 17 LPEYSNPLSKRNIYTPCLNS*TCTDKANKQHDNKIE*FKISRFIN 151


>BP071543 
          Length = 408

 Score = 26.6 bits (57), Expect = 4.3
 Identities = 13/37 (35%), Positives = 17/37 (45%)
 Frame = +3

Query: 33  EAYKKYILVSLIRHGQFSTSLPKYASPLAQRNLKLFC 69
           + +  Y L   I H    TS P+Y  P  +RNL   C
Sbjct: 195 QLHATYTLYCSIYHTHCRTSPPQYPLPACRRNLGSLC 305


>TC8174 similar to GB|AAO24559.1|27808558|BT003127 At5g39890 {Arabidopsis
           thaliana;}, partial (24%)
          Length = 1091

 Score = 26.6 bits (57), Expect = 4.3
 Identities = 10/24 (41%), Positives = 13/24 (53%)
 Frame = +1

Query: 206 TDEQISCDQLYLSKVSVQSFSFGY 229
           T    SCD + LS +    FSFG+
Sbjct: 283 TQSSCSCDHMLLSDIPTDPFSFGF 354


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.320    0.133    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,475,401
Number of Sequences: 28460
Number of extensions: 37217
Number of successful extensions: 177
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of query: 250
length of database: 4,897,600
effective HSP length: 88
effective length of query: 162
effective length of database: 2,393,120
effective search space: 387685440
effective search space used: 387685440
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)


Medicago: description of AC138464.6