
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138464.6 + phase: 0
(250 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC15755 similar to UP|AAP86661 (AAP86661) 26S proteasome subunit... 33 0.046
TC8654 similar to UP|AAP86661 (AAP86661) 26S proteasome subunit ... 31 0.18
TC12487 similar to UP|Q9SAL0 (Q9SAL0) T8K14.20 protein (At1g7938... 31 0.23
TC10170 similar to UP|PSD3_DAUCA (Q06364) Probable 26S proteasom... 30 0.39
AV777154 29 0.87
BP071543 27 4.3
TC8174 similar to GB|AAO24559.1|27808558|BT003127 At5g39890 {Ara... 27 4.3
>TC15755 similar to UP|AAP86661 (AAP86661) 26S proteasome subunit RPN6a,
partial (30%)
Length = 540
Score = 33.1 bits (74), Expect = 0.046
Identities = 23/101 (22%), Positives = 47/101 (45%), Gaps = 4/101 (3%)
Frame = +2
Query: 109 KQVVSSMYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQKD 168
+ + ++ ++N+ RL + + + + IA + L + E + QMI D + T++Q
Sbjct: 80 QSLYGTLMEQNLCRLIEPFSRVEIAHIAELIEL-PIEHVERKLSQMILDKKFAGTLDQGA 256
Query: 169 GMVRFLEDPE----QYKTCEMIEHIDSSIQRIMALSKKLTA 205
G + +DP+ T E I +I + + S K+ A
Sbjct: 257 GCLIIFDDPKTDAIYPATLETISNIGKVVDSLYVRSAKIMA 379
>TC8654 similar to UP|AAP86661 (AAP86661) 26S proteasome subunit RPN6a,
partial (31%)
Length = 615
Score = 31.2 bits (69), Expect = 0.18
Identities = 22/101 (21%), Positives = 46/101 (44%), Gaps = 4/101 (3%)
Frame = +3
Query: 109 KQVVSSMYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDGEIYATINQKD 168
+ + ++ ++N+ RL + + + + IA + L E + QMI D + T++Q
Sbjct: 93 QSLYGTLMEQNLCRLIEPFSRVEIAHIAELIEL-PIDHVERKLSQMILDKKFAGTLDQGA 269
Query: 169 GMVRFLEDPE----QYKTCEMIEHIDSSIQRIMALSKKLTA 205
G + +DP+ T E I +I + + S ++ A
Sbjct: 270 GCLIIFDDPQTDAIYPATLETISNIGKVVDSLYVRSAQIMA 392
>TC12487 similar to UP|Q9SAL0 (Q9SAL0) T8K14.20 protein
(At1g79380/T8K14_20), partial (57%)
Length = 1091
Score = 30.8 bits (68), Expect = 0.23
Identities = 22/94 (23%), Positives = 46/94 (48%)
Frame = +2
Query: 99 FEADNNLGLAKQVVSSMYKRNIQRLTQTYLTLSLEDIANTVHLNSAKEAEMHVLQMIQDG 158
F +D++ Q V + Y++ + L + T I + + + HVL +I DG
Sbjct: 17 FHSDHSPCHGFQEVLACYQKIVPNLKLSGPTSYAPVIEAAIDIVEKSRGQFHVLVVIADG 196
Query: 159 EIYATINQKDGMVRFLEDPEQYKTCEMIEHIDSS 192
++ +++ +DG + P++ KT + I +D+S
Sbjct: 197 QVTRSVDTEDGEL----SPQEEKTIKAI--VDAS 280
>TC10170 similar to UP|PSD3_DAUCA (Q06364) Probable 26S proteasome non-ATPase
regulatory subunit 3 (26S proteasome subunit S3) (Nuclear
antigen 21D7), partial (79%)
Length = 1197
Score = 30.0 bits (66), Expect = 0.39
Identities = 18/73 (24%), Positives = 32/73 (43%)
Frame = +3
Query: 72 PCIELAQTYNNGKVAEVEAFVNANAGRFEADNNLGLAKQVVSSMYKRNIQRLTQTYLTLS 131
P EL G + + A F AD L ++ + + ++ ++ +Y +S
Sbjct: 933 PYFELTNAVRIGDLELFRNIADKFATTFNADGTHNLIVRLRHIVIRTGLRNISISYSRIS 1112
Query: 132 LEDIANTVHLNSA 144
L D+A + LNSA
Sbjct: 1113 LADVARKLRLNSA 1151
>AV777154
Length = 373
Score = 28.9 bits (63), Expect = 0.87
Identities = 14/45 (31%), Positives = 27/45 (59%), Gaps = 4/45 (8%)
Frame = +2
Query: 53 LPKYASPLAQRNLKLFCQN--PCIELAQTYNNGKVA--EVEAFVN 93
LP+Y++PL++RN+ C N C + A ++ K+ ++ F+N
Sbjct: 17 LPEYSNPLSKRNIYTPCLNS*TCTDKANKQHDNKIE*FKISRFIN 151
>BP071543
Length = 408
Score = 26.6 bits (57), Expect = 4.3
Identities = 13/37 (35%), Positives = 17/37 (45%)
Frame = +3
Query: 33 EAYKKYILVSLIRHGQFSTSLPKYASPLAQRNLKLFC 69
+ + Y L I H TS P+Y P +RNL C
Sbjct: 195 QLHATYTLYCSIYHTHCRTSPPQYPLPACRRNLGSLC 305
>TC8174 similar to GB|AAO24559.1|27808558|BT003127 At5g39890 {Arabidopsis
thaliana;}, partial (24%)
Length = 1091
Score = 26.6 bits (57), Expect = 4.3
Identities = 10/24 (41%), Positives = 13/24 (53%)
Frame = +1
Query: 206 TDEQISCDQLYLSKVSVQSFSFGY 229
T SCD + LS + FSFG+
Sbjct: 283 TQSSCSCDHMLLSDIPTDPFSFGF 354
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.133 0.370
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,475,401
Number of Sequences: 28460
Number of extensions: 37217
Number of successful extensions: 177
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 177
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 177
length of query: 250
length of database: 4,897,600
effective HSP length: 88
effective length of query: 162
effective length of database: 2,393,120
effective search space: 387685440
effective search space used: 387685440
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC138464.6