BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138464.5 + phase: 0
(235 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV778530 216 4e-57
TC15519 30 0.47
TC12162 similar to AAS09999 (AAS09999) MYB transcription factor,... 29 0.62
TC10784 weakly similar to UP|BAD01556 (BAD01556) ERF-like protei... 29 0.62
AV427800 29 0.81
BP067897 29 0.81
TC8577 similar to UP|Q9XE94 (Q9XE94) Geranylgeranyl hydrogenase,... 28 1.1
TC14217 28 1.1
AV776400 28 1.8
TC9079 weakly similar to UP|Q8W4G1 (Q8W4G1) UDP-glucose glucosyl... 28 1.8
TC16374 similar to UP|Q7LZA6 (Q7LZA6) Protamine II (Fragment), p... 27 2.4
TC15312 UP|RPOD_LOTJA (Q9BBS7) DNA-directed RNA polymerase beta'... 27 4.0
BP052274 27 4.0
TC15930 similar to UP|O82520 (O82520) 3-oxoacyl-[acyl-carrier-pr... 26 5.2
TC8260 similar to UP|Q94ES8 (Q94ES8) Nodule extensin (Fragment),... 26 6.8
BP058029 26 6.8
TC19609 26 6.8
TC15525 UP|Q40209 (Q40209) RAB5A, complete 26 6.8
TC20167 26 6.8
BP069479 26 6.8
>AV778530
Length = 596
Score = 216 bits (549), Expect = 4e-57
Identities = 105/163 (64%), Positives = 136/163 (83%)
Frame = +2
Query: 10 QIQGLSSSSADIARLHTILKHSDETLRSDSSRLYPILQLIDPSIHSLGFLYLLDAFSAAS 69
++QGLSS+S DIARLH ILK +D++LRS+S+RL P+ + PS H+LGFLY+L+AF++ S
Sbjct: 110 KVQGLSSTSGDIARLHIILKQADDSLRSESTRLSPLFSQLHPSHHTLGFLYVLEAFTSNS 289
Query: 70 VSNQQQAEEAVPIIARFINACSVEQIRLAPEKFVSVCKRLKDLVTLLEAPIRGVAPMFTA 129
+S ++QAE +P++ F+ ACSVEQIRLAPEKF+ VCKRLKDLV LLEAP+RGV P+ TA
Sbjct: 290 IS-KEQAETVIPVMTGFVAACSVEQIRLAPEKFIWVCKRLKDLVMLLEAPMRGVGPLLTA 466
Query: 130 LRKLQVSAEHLTPLHAEFLMLCLLAKCYKTGLSILDDDVFEVD 172
+RKLQ+S HLTPLH+EFL LCL AKCYKTG SIL+D V E++
Sbjct: 467 VRKLQLSPAHLTPLHSEFLQLCL*AKCYKTGFSILEDGVTEIE 595
>TC15519
Length = 491
Score = 29.6 bits (65), Expect = 0.47
Identities = 18/54 (33%), Positives = 26/54 (47%), Gaps = 7/54 (12%)
Frame = +2
Query: 147 FLMLCLLAKCYKTGLSILDDDVFEVDHPRDLYLYCYYGCVA-------FFFSFE 193
FL+L L+ T L+IL + +DH RDL C + + F FSF+
Sbjct: 197 FLLLAWLSDLSSTFLTILMHPAYLIDHLRDLLNICIWAYIPHLHHLMNFLFSFD 358
>TC12162 similar to AAS09999 (AAS09999) MYB transcription factor, partial
(56%)
Length = 751
Score = 29.3 bits (64), Expect = 0.62
Identities = 18/52 (34%), Positives = 27/52 (51%)
Frame = -2
Query: 4 LEPLIAQIQGLSSSSADIARLHTILKHSDETLRSDSSRLYPILQLIDPSIHS 55
LEP+I I + S R+ TI ++ D+T +D+ L+ L L D HS
Sbjct: 501 LEPIINIIALMIVSPLQCERISTIPRNWDQTCFNDTHILFKFLVLFDHITHS 346
>TC10784 weakly similar to UP|BAD01556 (BAD01556) ERF-like protein, partial
(11%)
Length = 441
Score = 29.3 bits (64), Expect = 0.62
Identities = 12/43 (27%), Positives = 20/43 (45%)
Frame = +3
Query: 184 GCVAFFFSFELEKRDRCRICSYISVLHFSYITYCNFFNILDLF 226
GC +FFS + C++ + +F + +CNF L F
Sbjct: 255 GCSCYFFSHVILCASVCKLTLLVKCCYFDFQVFCNFHFTLFTF 383
>AV427800
Length = 246
Score = 28.9 bits (63), Expect = 0.81
Identities = 20/65 (30%), Positives = 31/65 (46%), Gaps = 1/65 (1%)
Frame = +3
Query: 25 HTILKHSDETLRSDSSRLYPILQLIDPSIHSLGFLY-LLDAFSAASVSNQQQAEEAVPII 83
H +L + T +S +Y + P+IH F + F + VSNQ+ E AV +
Sbjct: 12 HELLDTAISTALKESKPVYISISCNLPAIHHPTFARDPVPFFLSPKVSNQEGLEAAVEVA 191
Query: 84 ARFIN 88
A F+N
Sbjct: 192AAFLN 206
>BP067897
Length = 500
Score = 28.9 bits (63), Expect = 0.81
Identities = 19/59 (32%), Positives = 28/59 (47%), Gaps = 6/59 (10%)
Frame = -1
Query: 168 VFEVDHPRDLYLYCYYGCVAFFFSFELEK-RDRCRICSYISVLHFSYIT-----YCNFF 220
+F HP L + CY+ + FFF + L K ++ C + VL F IT C +F
Sbjct: 209 LFPQVHPYFLTVTCYFSVLTFFFWYNLSKLKNLCSV*IMPFVLCFVKITINTNMICKYF 33
>TC8577 similar to UP|Q9XE94 (Q9XE94) Geranylgeranyl hydrogenase, partial
(53%)
Length = 790
Score = 28.5 bits (62), Expect = 1.1
Identities = 20/60 (33%), Positives = 28/60 (46%), Gaps = 9/60 (15%)
Frame = -1
Query: 99 PEKFVSVCKRLKDL--------VTLLEAPIRGVAPMFTALRKL-QVSAEHLTPLHAEFLM 149
P F +C +K + + E PI + P+ LR + QVS +HLTP H LM
Sbjct: 631 PTSFAVICGVMKHIRRHLIHRNIHFQEQPIDNLRPIL--LRPVPQVSIQHLTPNHTNILM 458
>TC14217
Length = 985
Score = 28.5 bits (62), Expect = 1.1
Identities = 19/51 (37%), Positives = 22/51 (42%), Gaps = 1/51 (1%)
Frame = -2
Query: 185 CVAFFFSFELEKRDRCRICSYISVLHFSYIT-YCNFFNILDLFFTSFSIKC 234
C F F D +C +S+L F I C FNILDL FS C
Sbjct: 309 CQCFSNRFTYGSGDFIHLC--VSILFFLVINCLCVIFNILDLHTFCFSCSC 163
>AV776400
Length = 543
Score = 27.7 bits (60), Expect = 1.8
Identities = 16/57 (28%), Positives = 27/57 (47%)
Frame = -3
Query: 168 VFEVDHPRDLYLYCYYGCVAFFFSFELEKRDRCRICSYISVLHFSYITYCNFFNILD 224
+F VD + +YL +G + ++ +C+ S+ HFS I FF I+D
Sbjct: 403 LFSVDILKTVYL*VRFGWQIRYHLQLVQTSLEYMVCTLSSISHFSIINSLKFFLIID 233
>TC9079 weakly similar to UP|Q8W4G1 (Q8W4G1) UDP-glucose
glucosyltransferase, partial (65%)
Length = 1585
Score = 27.7 bits (60), Expect = 1.8
Identities = 22/83 (26%), Positives = 35/83 (41%), Gaps = 11/83 (13%)
Frame = +1
Query: 110 KDLVTLLEAPIRG-VAPMFTALRKLQVSAEHLTPLHAEFLMLCLLAKCYKTGLSILDDDV 168
K L +P++G + P+F + L + H+T +H E+ L+ L L D V
Sbjct: 13 KPHAVLTPSPLQGHINPLFQLAKLLHLRGFHITFVHTEYNHKRLIKSRGPNALDGLPDFV 192
Query: 169 FEV----------DHPRDLYLYC 181
FE + +DLY C
Sbjct: 193 FETIPDGLEDGDGEVSQDLYSLC 261
>TC16374 similar to UP|Q7LZA6 (Q7LZA6) Protamine II (Fragment), partial
(63%)
Length = 1130
Score = 27.3 bits (59), Expect = 2.4
Identities = 28/101 (27%), Positives = 44/101 (42%), Gaps = 1/101 (0%)
Frame = -2
Query: 135 VSAEHLTPLHAEFLMLCLLAKCYKTGLSILDDDVFEVDHPRDLYLYCYYGCVAFFFSFEL 194
V +HLT + CLL + D +F + L+L+C+ V FFF F
Sbjct: 820 VENKHLTSHSFRLFLACLLVDRI---FLVFDLSLFFFPN---LFLFCFSPLVFFFFFF-- 665
Query: 195 EKRDRCRICSYISVLHFSYITYCNFFNILDLFFT-SFSIKC 234
C S SV + ++ + F+I L F+ +FS+ C
Sbjct: 664 -----CTFSSVSSV--WISVSESSSFSISPLMFSMAFSLPC 563
>TC15312 UP|RPOD_LOTJA (Q9BBS7) DNA-directed RNA polymerase beta'' chain
(PEP) (Plastid-encoded RNA polymerase beta'' subunit)
(RNA polymerase beta'' subunit) , partial (84%)
Length = 3522
Score = 26.6 bits (57), Expect = 4.0
Identities = 11/41 (26%), Positives = 22/41 (52%)
Frame = -3
Query: 191 SFELEKRDRCRICSYISVLHFSYITYCNFFNILDLFFTSFS 231
S+ + R + + + V+H Y+ + FF+ L+ FF + S
Sbjct: 1654 SYRITGRTKTKYFFLVVVIHCIYLQFVFFFDSLEFFFFTIS 1532
>BP052274
Length = 563
Score = 26.6 bits (57), Expect = 4.0
Identities = 13/34 (38%), Positives = 18/34 (52%), Gaps = 2/34 (5%)
Frame = +3
Query: 160 GLSILDDDVFEVDHPRDLY--LYCYYGCVAFFFS 191
G L DV+ + H D + L CY+ V F+FS
Sbjct: 387 GEQTLSRDVYRLGHRFDFFRMLSCYFTTVGFYFS 488
>TC15930 similar to UP|O82520 (O82520) 3-oxoacyl-[acyl-carrier-protein]
synthase , partial (16%)
Length = 695
Score = 26.2 bits (56), Expect = 5.2
Identities = 15/44 (34%), Positives = 20/44 (45%), Gaps = 11/44 (25%)
Frame = +2
Query: 200 CRICSYISVL-----HFSYITYCNFFN------ILDLFFTSFSI 232
C CS++ + FSY+ C FF IL LFF S +
Sbjct: 392 CTFCSFLKFIWVWWTQFSYLQLCPFFYISNVTFILSLFFLSIFV 523
>TC8260 similar to UP|Q94ES8 (Q94ES8) Nodule extensin (Fragment), partial
(79%)
Length = 1017
Score = 25.8 bits (55), Expect = 6.8
Identities = 12/32 (37%), Positives = 16/32 (49%)
Frame = +3
Query: 187 AFFFSFELEKRDRCRICSYISVLHFSYITYCN 218
A+ F + E R+ C C YIS+ F CN
Sbjct: 807 AYLFDYCSEDREDC--CVYISLFQFQLCVCCN 896
>BP058029
Length = 580
Score = 25.8 bits (55), Expect = 6.8
Identities = 11/28 (39%), Positives = 15/28 (53%)
Frame = +3
Query: 177 LYLYCYYGCVAFFFSFELEKRDRCRICS 204
L + CYYGC F + ++ D C I S
Sbjct: 480 LIISCYYGC-CFNYFLQMNYEDFCHIAS 560
>TC19609
Length = 529
Score = 25.8 bits (55), Expect = 6.8
Identities = 12/48 (25%), Positives = 24/48 (50%)
Frame = +2
Query: 177 LYLYCYYGCVAFFFSFELEKRDRCRICSYISVLHFSYITYCNFFNILD 224
L+ +C + + FF++ R+ C + S++ T+C NIL+
Sbjct: 341 LFFFCCFPALLFFWA---H*RELCSLAFVSSIIFNISFTFCKKINILE 475
>TC15525 UP|Q40209 (Q40209) RAB5A, complete
Length = 1176
Score = 25.8 bits (55), Expect = 6.8
Identities = 11/43 (25%), Positives = 23/43 (52%)
Frame = -3
Query: 180 YCYYGCVAFFFSFELEKRDRCRICSYISVLHFSYITYCNFFNI 222
YC+ V FF +F ++ + + + +L FS++ + FF +
Sbjct: 130 YCFSLTVGFFATFCIKTQSFYMLLLLLCLLSFSFLLHSFFFTL 2
>TC20167
Length = 486
Score = 25.8 bits (55), Expect = 6.8
Identities = 16/39 (41%), Positives = 20/39 (51%), Gaps = 4/39 (10%)
Frame = +3
Query: 156 CYKTGLSIL---DDDVFEVDH-PRDLYLYCYYGCVAFFF 190
C K GL +L + EV H P+ YLY Y C F+F
Sbjct: 330 CKKIGLLLLLMPKLKIGEVGHLPKVYYLYIYVFCFVFWF 446
>BP069479
Length = 544
Score = 25.8 bits (55), Expect = 6.8
Identities = 16/39 (41%), Positives = 20/39 (51%), Gaps = 4/39 (10%)
Frame = -3
Query: 156 CYKTGLSIL---DDDVFEVDH-PRDLYLYCYYGCVAFFF 190
C K GL +L + EV H P+ YLY Y C F+F
Sbjct: 155 CKKIGLLLLLMPKLKIGEVGHLPKVYYLYIYVFCFVFWF 39
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.329 0.142 0.425
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,460,499
Number of Sequences: 28460
Number of extensions: 69052
Number of successful extensions: 583
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 577
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 582
length of query: 235
length of database: 4,897,600
effective HSP length: 87
effective length of query: 148
effective length of database: 2,421,580
effective search space: 358393840
effective search space used: 358393840
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 54 (25.4 bits)
Medicago: description of AC138464.5