BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138130.5 + phase: 0 /pseudo
(152 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AW720237 182 2e-47
TC18168 homologue to UP|Q8GSN4 (Q8GSN4) Non-cell-autonomous heat... 175 3e-45
BP041494 94 1e-39
TC9795 homologue to UP|Q9ATB8 (Q9ATB8) BiP-isoform D (Fragment),... 156 2e-39
BP083571 104 8e-24
BP044919 97 2e-21
AV769455 66 2e-12
BP064898 52 3e-08
BP040870 46 3e-06
BP058318 45 7e-06
AV406566 26 2.6
BE122619 26 3.4
BP038410 25 4.5
AV768665 25 4.5
NP840539 hypothetical protein [Lotus corniculatus var. japonicus] 24 10.0
NP566648 hypothetical protein [Lotus japonicus] 24 10.0
>AW720237
Length = 459
Score = 182 bits (463), Expect = 2e-47
Identities = 90/140 (64%), Positives = 109/140 (77%), Gaps = 1/140 (0%)
Frame = +3
Query: 5 SVVGIDLGTTYSCIAVWLDENRRVEIIHNDQGNRTTPSFVSFTKDQRLIGDAAKGQATSD 64
+ +GIDLGTTYSC+ VW+ N RVEII NDQGNRTTPS+V FT+ +RLIGDAAK Q +
Sbjct: 39 TAIGIDLGTTYSCVGVWM--NDRVEIIANDQGNRTTPSYVGFTESERLIGDAAKNQVARN 212
Query: 65 PENTVFDAKRLIGRKFSDSVVQNDIMLWPFRV-IGVDDKPMIIVKYKGQEKRFCPEEISS 123
P NTVFDAKRLIGRKF D +VQ DI LWPF+V G D KP+I VKYKG+ K+F E++SS
Sbjct: 213 PINTVFDAKRLIGRKFDDPIVQKDIKLWPFKVEAGPDGKPLIAVKYKGETKKFHAEQVSS 392
Query: 124 MILTKMREVAEKFLMSPRKE 143
M+LTKM+E AE +L K+
Sbjct: 393 MVLTKMKETAEAYLNKTIKD 452
>TC18168 homologue to UP|Q8GSN4 (Q8GSN4) Non-cell-autonomous heat shock
cognate protein 70, partial (23%)
Length = 508
Score = 175 bits (444), Expect = 3e-45
Identities = 89/137 (64%), Positives = 109/137 (78%), Gaps = 1/137 (0%)
Frame = +2
Query: 7 VGIDLGTTYSCIAVWLDENRRVEIIHNDQGNRTTPSFVSFTKDQRLIGDAAKGQATSDPE 66
+GIDLGTTYSC+ VW ++ RVEII NDQGNRTTPS+V+FT +RLIGDAAK Q +P
Sbjct: 86 IGIDLGTTYSCVGVW--QHDRVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPI 259
Query: 67 NTVFDAKRLIGRKFSDSVVQNDIMLWPFRV-IGVDDKPMIIVKYKGQEKRFCPEEISSMI 125
NTVFDAKRLIGR+ SD+ VQ+D+ LWPF+V G +KPMI V YKG++K+F EEISSM+
Sbjct: 260 NTVFDAKRLIGRRVSDASVQSDMKLWPFKVNSGPAEKPMIQVSYKGEDKQFAAEEISSMV 439
Query: 126 LTKMREVAEKFLMSPRK 142
L KMRE+AE +L S K
Sbjct: 440 LMKMREIAEAYLGSTIK 490
>BP041494
Length = 572
Score = 93.6 bits (231), Expect(2) = 1e-39
Identities = 47/73 (64%), Positives = 58/73 (79%), Gaps = 1/73 (1%)
Frame = +3
Query: 71 DAKRLIGRKFSDSVVQNDIMLWPFRVI-GVDDKPMIIVKYKGQEKRFCPEEISSMILTKM 129
DAKRLIGRKFSD V++D+ LWPF+VI G D+PMI+V YK +EK+F EEISSM+L KM
Sbjct: 243 DAKRLIGRKFSDDSVESDMKLWPFKVISGPVDRPMIVVNYKNEEKQFTAEEISSMVLIKM 422
Query: 130 REVAEKFLMSPRK 142
RE+AE +L S K
Sbjct: 423 REIAEAYLGSTVK 461
Score = 84.3 bits (207), Expect(2) = 1e-39
Identities = 41/57 (71%), Positives = 47/57 (81%)
Frame = +1
Query: 7 VGIDLGTTYSCIAVWLDENRRVEIIHNDQGNRTTPSFVSFTKDQRLIGDAAKGQATS 63
+GIDLGTTYSC+ VW +N RVEII NDQGNRTTPS+V+FT +RLIGDAAK Q S
Sbjct: 88 IGIDLGTTYSCVGVW--QNDRVEIIPNDQGNRTTPSYVAFTDFERLIGDAAKNQMRS 252
>TC9795 homologue to UP|Q9ATB8 (Q9ATB8) BiP-isoform D (Fragment), partial
(63%)
Length = 981
Score = 156 bits (394), Expect = 2e-39
Identities = 80/134 (59%), Positives = 99/134 (73%), Gaps = 1/134 (0%)
Frame = +1
Query: 5 SVVGIDLGTTYSCIAVWLDENRRVEIIHNDQGNRTTPSFVSFTKDQRLIGDAAKGQATSD 64
+V+GIDLGTTYSC+ V+ N VEII NDQGNR TPS+V+F +RLIG+AAK Q +
Sbjct: 148 TVIGIDLGTTYSCVGVY--RNGHVEIIANDQGNRITPSWVAFNDGERLIGEAAKNQFAVN 321
Query: 65 PENTVFDAKRLIGRKFSDSVVQNDIMLWPFRVIGVDDKPMIIVKYK-GQEKRFCPEEISS 123
PE T+FD KRLIGRKF D VQ D+ L P++++ D KP I VK K G+ K F PEE+S+
Sbjct: 322 PERTIFDVKRLIGRKFEDKEVQRDMKLVPYKIVNKDGKPYIQVKIKDGETKVFSPEEVSA 501
Query: 124 MILTKMREVAEKFL 137
MILTKM+E AE FL
Sbjct: 502 MILTKMKETAEAFL 543
>BP083571
Length = 423
Score = 104 bits (259), Expect = 8e-24
Identities = 47/63 (74%), Positives = 55/63 (86%)
Frame = +1
Query: 7 VGIDLGTTYSCIAVWLDENRRVEIIHNDQGNRTTPSFVSFTKDQRLIGDAAKGQATSDPE 66
+GIDLGTTYSC+AVW ++N R EIIHN+QGNRTTPSFV+FT QRLIGDAAK QA ++P
Sbjct: 172 IGIDLGTTYSCVAVWQEQNDRAEIIHNEQGNRTTPSFVAFTDTQRLIGDAAKNQAATNPT 351
Query: 67 NTV 69
NTV
Sbjct: 352 NTV 360
>BP044919
Length = 519
Score = 96.7 bits (239), Expect = 2e-21
Identities = 45/67 (67%), Positives = 57/67 (84%), Gaps = 1/67 (1%)
Frame = +3
Query: 77 GRKFSDSVVQNDIMLWPFRVI-GVDDKPMIIVKYKGQEKRFCPEEISSMILTKMREVAEK 135
GRK+SDSV+Q D+ LWPF+VI G DDKPMI+VK+KG+EK+ PEEISSM+L+KM+E+AE
Sbjct: 27 GRKYSDSVIQEDVKLWPFKVIAGTDDKPMIVVKHKGEEKKLVPEEISSMVLSKMKEIAEA 206
Query: 136 FLMSPRK 142
FL S K
Sbjct: 207 FLESSVK 227
>AV769455
Length = 556
Score = 66.2 bits (160), Expect = 2e-12
Identities = 32/93 (34%), Positives = 55/93 (58%), Gaps = 1/93 (1%)
Frame = +1
Query: 53 IGDAAKGQATSDPENTVFDAKRLIGRKFSDSVVQNDIMLWPFRVI-GVDDKPMIIVKYKG 111
+G A +P+N++ KRLIGR+F+D +Q D+ PF V G D P+I +Y G
Sbjct: 4 LGTAGASSTMMNPKNSISQLKRLIGRQFADPELQRDLKSLPFAVTEGPDGFPLIHARYLG 183
Query: 112 QEKRFCPEEISSMILTKMREVAEKFLMSPRKEC 144
+ + F P ++ M+L+ ++E+A+K L + +C
Sbjct: 184 ETRTFTPTQVFGMMLSNLKEIAQKNLNAAVVDC 282
>BP064898
Length = 542
Score = 52.4 bits (124), Expect = 3e-08
Identities = 26/66 (39%), Positives = 42/66 (63%), Gaps = 1/66 (1%)
Frame = +1
Query: 73 KRLIGRKFSDSVVQNDIMLWPFRVI-GVDDKPMIIVKYKGQEKRFCPEEISSMILTKMRE 131
KRLIG +FSD +Q D+ PF V G D P+I +Y G+ + F P ++ +M+L+ ++E
Sbjct: 337 KRLIGIQFSDPQLQRDLKSLPFLVTEGPDGYPLIHARYLGETRTFTPTQVFAMMLSNLKE 516
Query: 132 VAEKFL 137
+ +K L
Sbjct: 517 IXQKNL 534
>BP040870
Length = 505
Score = 45.8 bits (107), Expect = 3e-06
Identities = 20/31 (64%), Positives = 26/31 (83%)
Frame = +2
Query: 107 VKYKGQEKRFCPEEISSMILTKMREVAEKFL 137
V YKG+EK+F EEISSM+L KM+E+AE +L
Sbjct: 5 VNYKGEEKQFSAEEISSMVLMKMKEIAEAYL 97
>BP058318
Length = 555
Score = 44.7 bits (104), Expect = 7e-06
Identities = 34/132 (25%), Positives = 57/132 (42%), Gaps = 2/132 (1%)
Frame = +3
Query: 5 SVVGIDLGTTYSCIAV--WLDENRRVEIIHNDQGNRTTPSFVSFTKDQRLIGDAAKGQAT 62
+V +DLG+ +AV + I N+ R +P+ VSF RL+G+ A G
Sbjct: 147 AVFSVDLGSESVKVAVVNLKPGQSPISIAINEMSKRKSPALVSFHDGDRLLGEEAAGLVA 326
Query: 63 SDPENTVFDAKRLIGRKFSDSVVQNDIMLWPFRVIGVDDKPMIIVKYKGQEKRFCPEEIS 122
P+ + LIG+ +S + D M PF D + + + + EE+
Sbjct: 327 RYPQKVYSQIRDLIGKPYSSAKSFLDSMYLPFDT-KEDSRGTVSFRIDENGTEYSSEELV 503
Query: 123 SMILTKMREVAE 134
+M+L +AE
Sbjct: 504 AMLLGYTAALAE 539
>AV406566
Length = 376
Score = 26.2 bits (56), Expect = 2.6
Identities = 8/23 (34%), Positives = 17/23 (73%)
Frame = +3
Query: 2 MTKSVVGIDLGTTYSCIAVWLDE 24
++ + G+D+GTT++ I WL++
Sbjct: 153 ISTTFTGMDIGTTFTAITGWLED 221
>BE122619
Length = 353
Score = 25.8 bits (55), Expect = 3.4
Identities = 17/54 (31%), Positives = 28/54 (51%), Gaps = 1/54 (1%)
Frame = -1
Query: 100 DDKPMIIVKYKGQEKRFCPEEISSMILTKMREVAEKFLMSPRKECSCY-RACLL 152
+D+P+I +K G R CP S + K ++ +FL+ S + +ACLL
Sbjct: 347 NDRPVISIKQLGLRPRSCPWATS--LPFKKIDLLPRFLIMKEPFASIFIKACLL 192
>BP038410
Length = 468
Score = 25.4 bits (54), Expect = 4.5
Identities = 9/16 (56%), Positives = 12/16 (74%)
Frame = -2
Query: 77 GRKFSDSVVQNDIMLW 92
GRK DS+V D++LW
Sbjct: 173 GRKIFDSLVDKDVVLW 126
>AV768665
Length = 258
Score = 25.4 bits (54), Expect = 4.5
Identities = 10/19 (52%), Positives = 14/19 (73%)
Frame = +3
Query: 27 RVEIIHNDQGNRTTPSFVS 45
R++ I+ND GNR PS +S
Sbjct: 99 RLQNIYNDSGNRIIPSLLS 155
>NP840539 hypothetical protein [Lotus corniculatus var. japonicus]
Length = 3654
Score = 24.3 bits (51), Expect = 10.0
Identities = 26/98 (26%), Positives = 42/98 (42%), Gaps = 1/98 (1%)
Frame = +1
Query: 47 TKDQRLIGDAAKGQATSDPENTVFDAKRLIGRKFSDSVVQNDIMLWPFRVIGVDDKPMII 106
T+ + ++GDA K A +DPE ++ + + + W GV +P I
Sbjct: 619 TEVEFIVGDATKFDAVTDPEPEQ-------TKEEESELYKPTVSTW-----GVFPRPGNI 762
Query: 107 VKYKGQEKRFCPEEISSMILTK-MREVAEKFLMSPRKE 143
K G + P E+ K MREV K L++ K+
Sbjct: 763 SKTFGGGRVIRPGEVLETDEEKAMREVRTKQLLAAYKK 876
>NP566648 hypothetical protein [Lotus japonicus]
Length = 1101
Score = 24.3 bits (51), Expect = 10.0
Identities = 26/98 (26%), Positives = 42/98 (42%), Gaps = 1/98 (1%)
Frame = +1
Query: 47 TKDQRLIGDAAKGQATSDPENTVFDAKRLIGRKFSDSVVQNDIMLWPFRVIGVDDKPMII 106
T+ + ++GDA K A +DPE ++ + + + W GV +P I
Sbjct: 451 TEVEFIVGDATKFDAVTDPEPEQ-------TKEEESELYKPTVSTW-----GVFPRPGNI 594
Query: 107 VKYKGQEKRFCPEEISSMILTK-MREVAEKFLMSPRKE 143
K G + P E+ K MREV K L++ K+
Sbjct: 595 SKTFGGGRVIRPGEVLETDEEKAMREVRTKQLLAAYKK 708
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.137 0.408
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,395,404
Number of Sequences: 28460
Number of extensions: 24400
Number of successful extensions: 142
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 133
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of query: 152
length of database: 4,897,600
effective HSP length: 82
effective length of query: 70
effective length of database: 2,563,880
effective search space: 179471600
effective search space used: 179471600
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC138130.5