BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC138087.10 + phase: 0
(242 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV768281 275 4e-75
TC18926 similar to AAQ63968 (AAQ63968) VAP27-1, partial (36%) 162 5e-41
TC16822 127 2e-30
TC16491 similar to GB|AAM62506.1|21553413|AY085274 VAMP (vesicle... 114 2e-26
TC15794 weakly similar to UP|Q9SC83 (Q9SC83) VAP27, partial (28%) 107 2e-24
TC8473 similar to UP|BAD11591 (BAD11591) 27k vesicle-associated ... 59 8e-10
AV767728 55 1e-08
TC10356 similar to GB|AAM62506.1|21553413|AY085274 VAMP (vesicle... 45 1e-05
AV770492 44 2e-05
TC11426 similar to UP|Q8LAZ8 (Q8LAZ8) Membrane associated protei... 43 4e-05
TC10996 similar to UP|Q9SKU1 (Q9SKU1) At2g20760 protein (At2g207... 31 0.22
BI418438 30 0.29
TC14223 similar to UP|Q8GZV0 (Q8GZV0) Obtusifoliol-14-demethylas... 30 0.29
TC17133 28 1.4
TC13531 similar to UP|O43989 (O43989) Homeobox-containing protei... 27 2.4
BP053203 27 2.4
TC12859 weakly similar to UP|CAE55354 (CAE55354) PE-PGRS FAMILY ... 27 2.4
TC17544 similar to PIR|T04919|T04919 DNA-binding protein homolog... 27 3.2
AW720051 27 4.1
TC14545 similar to UP|Q9FT78 (Q9FT78) P23 co-chaperone, partial ... 27 4.1
>AV768281
Length = 575
Score = 275 bits (704), Expect = 4e-75
Identities = 133/151 (88%), Positives = 141/151 (93%)
Frame = +3
Query: 5 GDLLSIEPLELKFPFELKKQISCSLQLSNKTDSYVAFKVKTTNPRKYCVRPNTGIVLPRS 64
GDLL I+PLELKF FELKKQISCSLQLSN+TD YVAFKVKTTNP+KYCVRPNTG+VLPRS
Sbjct: 123 GDLLHIDPLELKFIFELKKQISCSLQLSNQTDEYVAFKVKTTNPKKYCVRPNTGVVLPRS 302
Query: 65 TCDVMVTMQAQKEAPADMQCKDKFLLQSVKTNDGVSPKDISAEMFNKEAGHVVEECKLRV 124
TCDV VTMQAQKEAP DMQCKDKFLLQSVK DGV+ KDI+AEMFNKEAGH+VEECKLRV
Sbjct: 303 TCDVTVTMQAQKEAPPDMQCKDKFLLQSVKVEDGVTAKDITAEMFNKEAGHIVEECKLRV 482
Query: 125 VYVSPPQPPSPVPEGSEEGSSPRGSFSENGN 155
VYV+PPQPPSPV EGSEEGSSPRGS SENGN
Sbjct: 483 VYVAPPQPPSPVAEGSEEGSSPRGSVSENGN 575
>TC18926 similar to AAQ63968 (AAQ63968) VAP27-1, partial (36%)
Length = 419
Score = 162 bits (410), Expect = 5e-41
Identities = 78/90 (86%), Positives = 83/90 (91%)
Frame = +1
Query: 5 GDLLSIEPLELKFPFELKKQISCSLQLSNKTDSYVAFKVKTTNPRKYCVRPNTGIVLPRS 64
G LL I+PLELKF F+LKKQISCSLQL+NKTD YVAFKVKTTNP+KYCVRPNTG+VLPRS
Sbjct: 145 GYLLRIDPLELKFTFQLKKQISCSLQLTNKTDEYVAFKVKTTNPKKYCVRPNTGVVLPRS 324
Query: 65 TCDVMVTMQAQKEAPADMQCKDKFLLQSVK 94
TCDV VTMQAQKEAP DMQCKDKFLLQ VK
Sbjct: 325 TCDVKVTMQAQKEAPPDMQCKDKFLLQCVK 414
>TC16822
Length = 802
Score = 127 bits (319), Expect = 2e-30
Identities = 63/88 (71%), Positives = 76/88 (85%)
Frame = +2
Query: 154 GNANGPEFAQVTRGSAERPEAQDKSAEARALISRLTEEKNNAIQQTSRLRQELELLKREG 213
GN N + A + RG ER EA DKSAEA+ALISRLTEEK+NAIQQT++LRQEL+LL+RE
Sbjct: 2 GNVNVSDSATIARGFIERHEATDKSAEAKALISRLTEEKSNAIQQTNKLRQELDLLRRES 181
Query: 214 NRNRGGVSFIIVILIGLLGLIMGYLMKK 241
N+NRGGVS +IVIL+GLLG++MGYLMKK
Sbjct: 182 NKNRGGVSMLIVILVGLLGIVMGYLMKK 265
>TC16491 similar to GB|AAM62506.1|21553413|AY085274 VAMP (vesicle-associated
membrane protein)-associated protein-like {Arabidopsis
thaliana;}, partial (31%)
Length = 569
Score = 114 bits (285), Expect = 2e-26
Identities = 69/174 (39%), Positives = 98/174 (55%), Gaps = 3/174 (1%)
Frame = +3
Query: 70 VTMQAQKEAPADMQCKDKFLLQSVKTNDGVSPKDISAEMFNKEAGHVVEECKLRVVYVSP 129
VT+QAQ+E P DMQCKDKFLLQS N D+ + F+K++G+ +EE KLRV Y++P
Sbjct: 6 VTLQAQREFPPDMQCKDKFLLQSTIVNPNTDVDDLPPDTFSKDSGNSIEELKLRVAYITP 185
Query: 130 PQPPSPVPEGSEEGSSPRGSFSENGNANGPEFAQVTRGSAERPEAQDKSAEARALISRLT 189
+SP GS E+G N + K + AL +L
Sbjct: 186 --------------TSPDGSSEEDGLKNSAQ----------------KLGTSSAL-EQLK 272
Query: 190 EEKNNAIQQTSRLRQELELLKREGNRNRG---GVSFIIVILIGLLGLIMGYLMK 240
EE++ A +QT L+QEL++LKR+ NR G G SF + +GLLGL++G+L+K
Sbjct: 273 EERDVAARQTRMLQQELDMLKRQRNRRGGGDPGFSFTFAVFVGLLGLLLGFLLK 434
>TC15794 weakly similar to UP|Q9SC83 (Q9SC83) VAP27, partial (28%)
Length = 616
Score = 107 bits (266), Expect = 2e-24
Identities = 51/69 (73%), Positives = 64/69 (91%)
Frame = +1
Query: 174 AQDKSAEARALISRLTEEKNNAIQQTSRLRQELELLKREGNRNRGGVSFIIVILIGLLGL 233
A DKSAEA+ALI+RLTEEKNNAIQQ ++L QEL++L+RE N++RGG+S +IVILIGLLG+
Sbjct: 4 ASDKSAEAKALIARLTEEKNNAIQQNNKLSQELDMLRRESNKSRGGISMLIVILIGLLGI 183
Query: 234 IMGYLMKKA 242
IMGY+MKKA
Sbjct: 184 IMGYIMKKA 210
>TC8473 similar to UP|BAD11591 (BAD11591) 27k vesicle-associated membrane
protein-associated protein-like, partial (81%)
Length = 1232
Score = 58.9 bits (141), Expect = 8e-10
Identities = 41/136 (30%), Positives = 74/136 (54%), Gaps = 5/136 (3%)
Frame = +1
Query: 8 LSIEPL-ELKFPFELKKQISCSLQLSNKTDSYVAFKVKTTNPRKYCVRPNTGIVLPRSTC 66
L ++P +L FP+E KQ+ ++++ N + S+VAFK +TT P+ +RP I+ P +
Sbjct: 388 LKLDPSSKLYFPYEPGKQVRSAIRIKNTSKSHVAFKFQTTAPKSCFMRPPGAILAPGES- 564
Query: 67 DVMVTMQAQKEAPADMQCKD----KFLLQSVKTNDGVSPKDISAEMFNKEAGHVVEECKL 122
++ T+ E P + + + KF + S+K + D E+F+++ V E L
Sbjct: 565 -IIATVFKFVEQPENNEKPEKTGLKFKIMSLKVKGAM---DYVPELFDEQKDQVAVEQIL 732
Query: 123 RVVYVSPPQPPSPVPE 138
RV+++ P+ PSP E
Sbjct: 733 RVIFLD-PERPSPALE 777
>AV767728
Length = 332
Score = 55.1 bits (131), Expect = 1e-08
Identities = 33/98 (33%), Positives = 51/98 (51%), Gaps = 3/98 (3%)
Frame = -2
Query: 146 PRGSFSENGNANGPEFAQVTRGSAERPEAQDKSAEARALISRLTEEKNNAIQQTSRLRQE 205
P GSF ++G N AQ + + +L ++KN +QT L+QE
Sbjct: 331 PNGSFEKDGLKNS---------------AQKLGTFSSQALEQLKKKKNVGARQTRMLQQE 197
Query: 206 LELLKREGNRNRG---GVSFIIVILIGLLGLIMGYLMK 240
L++LKR+ NR G G SF + +G LGL++G+L+K
Sbjct: 196 LDMLKRQRNRRGGGDPGFSFTFAVFVGPLGLLLGFLLK 83
>TC10356 similar to GB|AAM62506.1|21553413|AY085274 VAMP (vesicle-associated
membrane protein)-associated protein-like {Arabidopsis
thaliana;}, partial (16%)
Length = 434
Score = 45.1 bits (105), Expect = 1e-05
Identities = 20/36 (55%), Positives = 27/36 (74%)
Frame = +3
Query: 7 LLSIEPLELKFPFELKKQISCSLQLSNKTDSYVAFK 42
L+S+ P EL+F FEL+KQ C L++ N T +YVAFK
Sbjct: 327 LISVNPDELRFNFELEKQTFCDLKVLNNTQNYVAFK 434
>AV770492
Length = 479
Score = 43.9 bits (102), Expect = 2e-05
Identities = 24/60 (40%), Positives = 39/60 (65%), Gaps = 4/60 (6%)
Frame = -2
Query: 185 ISRLTEEKNNAIQQTSRLRQELELLKREGNRNRG----GVSFIIVILIGLLGLIMGYLMK 240
+ +L EE++ A + T L L++LKR+ +R RG G SF + +GLLGL++G+L+K
Sbjct: 394 LEQLKEERDVAARPTRMLLPALDMLKRQRHR-RGVGDPGFSFTFAVFVGLLGLLLGFLLK 218
>TC11426 similar to UP|Q8LAZ8 (Q8LAZ8) Membrane associated protein-like
protein, partial (33%)
Length = 608
Score = 43.1 bits (100), Expect = 4e-05
Identities = 25/76 (32%), Positives = 39/76 (50%), Gaps = 4/76 (5%)
Frame = +3
Query: 14 ELKFPFELKKQISCSLQLSNKTDSYVAFKVKTTNPRKYCVRPNTGIVLPR----STCDVM 69
+L FP+E KQ+ ++ + N ++VAFK +TT P+ +RP GI+ P +T
Sbjct: 381 KLYFPYEPGKQVRSAIAIKNTCKTHVAFKFQTTAPKSCYMRPPGGILSPGESIIATVFKF 560
Query: 70 VTMQAQKEAPADMQCK 85
V E P D + K
Sbjct: 561 VEPPENNEKPLDQKSK 608
>TC10996 similar to UP|Q9SKU1 (Q9SKU1) At2g20760 protein
(At2g20760/F5H14.27), partial (12%)
Length = 581
Score = 30.8 bits (68), Expect = 0.22
Identities = 15/62 (24%), Positives = 27/62 (43%), Gaps = 5/62 (8%)
Frame = +3
Query: 127 VSPPQPPSPV-----PEGSEEGSSPRGSFSENGNANGPEFAQVTRGSAERPEAQDKSAEA 181
+ PP PP+P +G E S P G+ E + P A + + P+ + + E
Sbjct: 138 IPPPPPPAPAKDSKDKDGKETASKPNGTAPEGAPESQPRDAATNDSTTDLPQKEAPATEE 317
Query: 182 RA 183
++
Sbjct: 318 QS 323
>BI418438
Length = 431
Score = 30.4 bits (67), Expect = 0.29
Identities = 13/31 (41%), Positives = 16/31 (50%)
Frame = +1
Query: 129 PPQPPSPVPEGSEEGSSPRGSFSENGNANGP 159
PP PPSP P ++ S P + NAN P
Sbjct: 241 PPPPPSPTPPPRQKSSKPARPPASPSNANPP 333
>TC14223 similar to UP|Q8GZV0 (Q8GZV0) Obtusifoliol-14-demethylase, complete
Length = 1917
Score = 30.4 bits (67), Expect = 0.29
Identities = 21/60 (35%), Positives = 25/60 (41%), Gaps = 1/60 (1%)
Frame = +1
Query: 128 SPPQPPSPVPEGSEEGSSP-RGSFSENGNANGPEFAQVTRGSAERPEAQDKSAEARALIS 186
S PPSP P S SSP R S +A+ P A + S R +KS A S
Sbjct: 136 SSSSPPSPSPSSSSPSSSPNRRKTSPQFSADFPSSAASSASSKARSSCSEKSTRNSAASS 315
>TC17133
Length = 556
Score = 28.1 bits (61), Expect = 1.4
Identities = 16/65 (24%), Positives = 27/65 (40%), Gaps = 9/65 (13%)
Frame = -2
Query: 129 PPQPPSPVPEGSEEG---------SSPRGSFSENGNANGPEFAQVTRGSAERPEAQDKSA 179
PP PP P P G+ S P + + + P + R SA+ P +++
Sbjct: 426 PPPPPPPAPHGASAA*SKLSHRNPSPPSRTTAAISRNSAPALGRRRR*SAKPPHVPSRTS 247
Query: 180 EARAL 184
AR++
Sbjct: 246 RARSM 232
>TC13531 similar to UP|O43989 (O43989) Homeobox-containing protein
(Fragment), partial (5%)
Length = 434
Score = 27.3 bits (59), Expect = 2.4
Identities = 10/16 (62%), Positives = 11/16 (68%)
Frame = -3
Query: 120 CKLRVVYVSPPQPPSP 135
C L V Y+ PPQPP P
Sbjct: 378 CGLHVSYLQPPQPPVP 331
>BP053203
Length = 530
Score = 27.3 bits (59), Expect = 2.4
Identities = 26/76 (34%), Positives = 37/76 (48%), Gaps = 1/76 (1%)
Frame = +2
Query: 133 PSPVPEGSEEGSSPRGSFS-ENGNANGPEFAQVTRGSAERPEAQDKSAEARALISRLTEE 191
PSP+P + GSS + S S NG ++ P F+ T + E E D+S L EE
Sbjct: 314 PSPIPIRAS-GSSVKSSLSGHNGRSHRPNFSSGTSNTYE-DEVADRS---------LGEE 460
Query: 192 KNNAIQQTSRLRQELE 207
I T +R+E+E
Sbjct: 461 HKYEI--TESMRREME 502
>TC12859 weakly similar to UP|CAE55354 (CAE55354) PE-PGRS FAMILY PROTEIN,
partial (5%)
Length = 528
Score = 27.3 bits (59), Expect = 2.4
Identities = 14/44 (31%), Positives = 15/44 (33%)
Frame = -2
Query: 129 PPQPPSPVPEGSEEGSSPRGSFSENGNANGPEFAQVTRGSAERP 172
PP P P P + SSP SF P F G P
Sbjct: 203 PPPPFRPTPPAPPQASSPFSSFGPPVRPGAPSFRPAPPGRFNDP 72
>TC17544 similar to PIR|T04919|T04919 DNA-binding protein homolog T9A21.10 -
Arabidopsis thaliana {Arabidopsis thaliana;}, partial
(14%)
Length = 578
Score = 26.9 bits (58), Expect = 3.2
Identities = 17/50 (34%), Positives = 23/50 (46%)
Frame = -1
Query: 101 PKDISAEMFNKEAGHVVEECKLRVVYVSPPQPPSPVPEGSEEGSSPRGSF 150
P+ SA E + +E ++ V VSPP PP P +SP G F
Sbjct: 419 PESSSAPASEDEEAEI-DELRVVVARVSPPPPPPP-------ATSPSGGF 294
>AW720051
Length = 564
Score = 26.6 bits (57), Expect = 4.1
Identities = 10/18 (55%), Positives = 12/18 (66%)
Frame = +1
Query: 128 SPPQPPSPVPEGSEEGSS 145
+PP PP P P+ S GSS
Sbjct: 328 APPPPPPPEPQSSSGGSS 381
>TC14545 similar to UP|Q9FT78 (Q9FT78) P23 co-chaperone, partial (46%)
Length = 1127
Score = 26.6 bits (57), Expect = 4.1
Identities = 12/38 (31%), Positives = 20/38 (52%), Gaps = 1/38 (2%)
Frame = -2
Query: 129 PPQPPSPVPEGSEEGSSP-RGSFSENGNANGPEFAQVT 165
PP PP P+P E+ P GS + + +++ +Q T
Sbjct: 526 PPSPPIPIPPNFEKSIPPISGSATTSSSSSSTHLSQST 413
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.313 0.132 0.367
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,551,225
Number of Sequences: 28460
Number of extensions: 49430
Number of successful extensions: 600
Number of sequences better than 10.0: 63
Number of HSP's better than 10.0 without gapping: 547
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 585
length of query: 242
length of database: 4,897,600
effective HSP length: 88
effective length of query: 154
effective length of database: 2,393,120
effective search space: 368540480
effective search space used: 368540480
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 54 (25.4 bits)
Medicago: description of AC138087.10