BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137986.3 + phase: 0
(571 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC12893 similar to GB|AAP37728.1|30725412|BT008369 At3g03720 {Ar... 272 1e-73
TC18277 weakly similar to UP|Q9FIV7 (Q9FIV7) Cationic amino acid... 219 7e-58
TC19724 similar to UP|Q9ASS7 (Q9ASS7) AT5g36940/MLF18_60, partia... 155 1e-38
AW720091 150 7e-37
AV409908 102 1e-22
AV766986 37 0.011
TC12470 similar to UP|Q9LJH9 (Q9LJH9) Gb|AAD25781.1, partial (5%) 30 1.1
BP071340 29 1.8
TC16451 similar to UP|AAR20751 (AAR20751) At3g24570, partial (51%) 28 3.1
TC20077 28 3.1
AV779445 28 3.1
TC17939 weakly similar to UP|Q945P2 (Q945P2) AT5g49210/K21P3_8, ... 28 5.2
TC9431 weakly similar to UP|Q84UI1 (Q84UI1) Allergen len c 3.02 ... 27 9.0
TC17368 27 9.0
>TC12893 similar to GB|AAP37728.1|30725412|BT008369 At3g03720 {Arabidopsis
thaliana;}, partial (30%)
Length = 564
Score = 272 bits (695), Expect = 1e-73
Identities = 132/187 (70%), Positives = 155/187 (82%)
Frame = +3
Query: 19 KQVDSPRQNTKPLLAKELSVYHLIAIGVGSTIGAGVYVLVGTVAREHSGPALAISFLIAG 78
KQVDS LA++LS L+ IGVG+TIGAGVY+L+GTVARE +GPAL IS LIAG
Sbjct: 3 KQVDSVHVRGHAQLARKLSAVDLVGIGVGATIGAGVYILIGTVAREQAGPALVISLLIAG 182
Query: 79 LAAGLSAFCYAELACRCPSAGSAYHYSYICLGEGVAWLIGWALLLEYTIGSAAVARGITP 138
+AA LSA CYAELACRCPSAGSAYHY+YIC+GEGVAWL+GW+L+LEYTIG++AVARGITP
Sbjct: 183 IAAALSALCYAELACRCPSAGSAYHYTYICIGEGVAWLVGWSLILEYTIGASAVARGITP 362
Query: 139 NLAPLFGGLDNLPFFLSRQHIPGIDIVVDPCAAILVLLVTGLLCLGIKESTVVQGIVTSV 198
NLA FGG +NLP FL+R +PG+ IVVDPCAA L+L VT LLCLGIKES + Q IVT+V
Sbjct: 363 NLALFFGGQENLPVFLARHTLPGLGIVVDPCAAALILFVTLLLCLGIKESAMAQSIVTTV 542
Query: 199 NVCALLF 205
NV +LF
Sbjct: 543 NVIVMLF 563
>TC18277 weakly similar to UP|Q9FIV7 (Q9FIV7) Cationic amino acid
transporter-like protein, partial (19%)
Length = 557
Score = 219 bits (559), Expect = 7e-58
Identities = 117/179 (65%), Positives = 137/179 (76%), Gaps = 9/179 (5%)
Frame = +2
Query: 373 TGLAAAVLAFSMEVSDLAGMVSVGTLLAFTMVAISVLILRYIPPNKVPVPPSLQDSIV-- 430
TGL AAVLAF+MEVS+LAGMVSVGTLLAFT VAISVLILRYIPP+ VP+PPSLQ+ I
Sbjct: 5 TGLGAAVLAFTMEVSELAGMVSVGTLLAFTTVAISVLILRYIPPDVVPLPPSLQEPIEYS 184
Query: 431 ----EIKVEYEEENIGTSEVSKPLDVTRDFSIDYPLISKHRSKGNHL---NRRRVVGWTI 483
E + E N TS KPL V D +IDYP K+ + N++ NRR+ +GW I
Sbjct: 185 WSHSETNEKDTEANGATSGNKKPLLVKEDVTIDYPFNCKYLAVENYIHNGNRRKFIGWVI 364
Query: 484 AIICLGAFVLTYAASCLTFLSSVRFALCGVGGTLLVSGFVFLTCMDQDDARHDFGHSGG 542
A CLG FVLT+AAS +T LSS+RFALCG+GGTLL+SGFVFLTCM+QDDARH+FGHSGG
Sbjct: 365 AFTCLGVFVLTFAASNITLLSSLRFALCGIGGTLLLSGFVFLTCMEQDDARHNFGHSGG 541
>TC19724 similar to UP|Q9ASS7 (Q9ASS7) AT5g36940/MLF18_60, partial (8%)
Length = 428
Score = 155 bits (393), Expect = 1e-38
Identities = 90/143 (62%), Positives = 98/143 (67%), Gaps = 14/143 (9%)
Frame = +3
Query: 399 LAFTMVAISVLILRYIPPNKVPVPPSLQDSI-----------VEIKVEYEEENIGTSEVS 447
LAFT+VAI VLILRYIPPN+VP+PPS+QDSI E VEY E SE S
Sbjct: 3 LAFTIVAICVLILRYIPPNEVPLPPSIQDSITSVSTGYSLSSAESNVEYAEAYASISEDS 182
Query: 448 KPLDVTRDFSIDYPLISKHRSKGNHL---NRRRVVGWTIAIICLGAFVLTYAASCLTFLS 504
KPL V D SI YPLI+KH G +L NRRRVVGW+I ICLGAFVLTYA S + L
Sbjct: 183 KPLVVKEDVSIRYPLIAKHLGIGYYLNDGNRRRVVGWSITFICLGAFVLTYATSYIP-LI 359
Query: 505 SVRFALCGVGGTLLVSGFVFLTC 527
VRF LCGVGG LLVSG VFLTC
Sbjct: 360 FVRFVLCGVGGILLVSGLVFLTC 428
>AW720091
Length = 492
Score = 150 bits (378), Expect = 7e-37
Identities = 75/102 (73%), Positives = 87/102 (84%), Gaps = 3/102 (2%)
Frame = +3
Query: 14 FFTRRKQVDSP---RQNTKPLLAKELSVYHLIAIGVGSTIGAGVYVLVGTVAREHSGPAL 70
F TRRK+VDS +++ LAKEL+V HL+AIGVG+T+GAGVYVLVGTVAREH+GPAL
Sbjct: 186 FVTRRKKVDSAGTTSNDSENQLAKELTVPHLMAIGVGATVGAGVYVLVGTVAREHAGPAL 365
Query: 71 AISFLIAGLAAGLSAFCYAELACRCPSAGSAYHYSYICLGEG 112
+SFL+AG AA LSA CYAELA RCPSAGSAYHYSYIC+GEG
Sbjct: 366 PLSFLVAGFAAALSALCYAELASRCPSAGSAYHYSYICVGEG 491
>AV409908
Length = 427
Score = 102 bits (255), Expect = 1e-22
Identities = 52/143 (36%), Positives = 90/143 (62%), Gaps = 8/143 (5%)
Frame = +1
Query: 186 KESTVVQGIVTSVNVCALLFVIVAGGYMGFKSGWVGY------ELPTGYFPFGVDGMLAG 239
+ES+VV I+T++++ + FVIV G G W + E P+G+FP+G G+ G
Sbjct: 1 RESSVVNMILTALHILFIAFVIVIGFCRG---SWKNFTEPAHRENPSGFFPYGAAGVFKG 171
Query: 240 SATVFFAYVGFDAVASTAEEVKNPQRDLPLGIGASLFLCCGMYMLVSVVVVGLVPYYAID 299
+A V+ +Y+G+DAV++ AEEV++P +D+P+G+ S+ + +Y L++ + L+PY ID
Sbjct: 172 AAAVYMSYIGYDAVSTMAEEVRDPVKDIPIGVSGSVAIVTVLYCLMAASMSKLLPYDLID 351
Query: 300 PDTPISSAFS--DHGMQWAAYII 320
+ P S+AF+ G WA+ +I
Sbjct: 352 AEAPFSAAFNGRSDGWGWASRVI 420
>AV766986
Length = 541
Score = 36.6 bits (83), Expect = 0.011
Identities = 13/22 (59%), Positives = 16/22 (72%)
Frame = -2
Query: 523 VFLTCMDQDDARHDFGHSGGMS 544
+ L C +QDD RH FGHSGG +
Sbjct: 528 IVLACTEQDDTRHSFGHSGGFA 463
>TC12470 similar to UP|Q9LJH9 (Q9LJH9) Gb|AAD25781.1, partial (5%)
Length = 825
Score = 30.0 bits (66), Expect = 1.1
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 544 SYHHILVFVKFIWIVLGFVCIGRFEFIY 571
SY+HIL FV F++I + F C+ F+Y
Sbjct: 368 SYYHILDFVSFVYIGICFTCV----FVY 439
>BP071340
Length = 400
Score = 29.3 bits (64), Expect = 1.8
Identities = 12/18 (66%), Positives = 13/18 (71%)
Frame = -3
Query: 474 NRRRVVGWTIAIICLGAF 491
NR RV GW I+ ICLG F
Sbjct: 365 NRWRVAGWIISSICLGEF 312
>TC16451 similar to UP|AAR20751 (AAR20751) At3g24570, partial (51%)
Length = 654
Score = 28.5 bits (62), Expect = 3.1
Identities = 21/65 (32%), Positives = 30/65 (45%), Gaps = 2/65 (3%)
Frame = +2
Query: 232 GVDGMLAG--SATVFFAYVGFDAVASTAEEVKNPQRDLPLGIGASLFLCCGMYMLVSVVV 289
G DG L G VFF Y+GF ST + V + D+ +L L G++ +V V
Sbjct: 80 GADGFLFGPLDLLVFFTYMGF----STGKSVPQIKEDVKRDFLPALILEGGIWPVVQVAN 247
Query: 290 VGLVP 294
+P
Sbjct: 248 FRYIP 262
>TC20077
Length = 516
Score = 28.5 bits (62), Expect = 3.1
Identities = 23/80 (28%), Positives = 37/80 (45%), Gaps = 3/80 (3%)
Frame = +1
Query: 108 CLGEGVAWLIGWALLLEYTIGSAAVARGITPNLAPLFGGLDNLPFFLSRQHIPGIDIVVD 167
C+ + W+IG +L L +G AAV R I+ + GG + L + V+D
Sbjct: 151 CIHSSIFWVIGRSLFLLVWLGFAAVCRFISVVVVSN*GGYWPVLVLLVWHGCVVVCRVID 330
Query: 168 PCAAIL---VLLVTGLLCLG 184
C + + +LLV +C G
Sbjct: 331 TCLSPIKGVMLLVYLTVCCG 390
>AV779445
Length = 581
Score = 28.5 bits (62), Expect = 3.1
Identities = 11/22 (50%), Positives = 16/22 (72%)
Frame = -3
Query: 273 ASLFLCCGMYMLVSVVVVGLVP 294
A+L +CC Y+LV +VVG+ P
Sbjct: 228 AALLICCPAYVLVLAIVVGM*P 163
>TC17939 weakly similar to UP|Q945P2 (Q945P2) AT5g49210/K21P3_8, partial
(61%)
Length = 999
Score = 27.7 bits (60), Expect = 5.2
Identities = 10/30 (33%), Positives = 20/30 (66%)
Frame = +1
Query: 267 LPLGIGASLFLCCGMYMLVSVVVVGLVPYY 296
L +G+ LFLC +++LV +V + +P++
Sbjct: 859 LAMGVRTMLFLCSYLFILVVIVALMQIPFW 948
>TC9431 weakly similar to UP|Q84UI1 (Q84UI1) Allergen len c 3.02
(Fragment), partial (67%)
Length = 1328
Score = 26.9 bits (58), Expect = 9.0
Identities = 17/60 (28%), Positives = 29/60 (48%)
Frame = -1
Query: 366 PVKGTIITGLAAAVLAFSMEVSDLAGMVSVGTLLAFTMVAISVLILRYIPPNKVPVPPSL 425
P+ I+ LA + S DL S+ TLL V ++ +++ ++PP+ P P L
Sbjct: 314 PLSLASISLLALFFPSLSPSHQDLQDGESLSTLLLLLHVQVATMVVVHLPPSHPPGVPFL 135
>TC17368
Length = 563
Score = 26.9 bits (58), Expect = 9.0
Identities = 18/49 (36%), Positives = 27/49 (54%), Gaps = 1/49 (2%)
Frame = +1
Query: 42 IAIGVGSTIGAGVYVLVGTVAREHSGPALAISF-LIAGLAAGLSAFCYA 89
I + G++ AGV G +AR SG LAI+ LI +++ + A YA
Sbjct: 79 INVNFGASFKAGVGAGFGLIARNGSGQVLAIATNLIVDVSSPVVAQAYA 225
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.326 0.143 0.443
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 10,949,624
Number of Sequences: 28460
Number of extensions: 165869
Number of successful extensions: 1072
Number of sequences better than 10.0: 28
Number of HSP's better than 10.0 without gapping: 1052
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1065
length of query: 571
length of database: 4,897,600
effective HSP length: 95
effective length of query: 476
effective length of database: 2,193,900
effective search space: 1044296400
effective search space used: 1044296400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 58 (26.9 bits)
Medicago: description of AC137986.3