BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137825.12 - phase: 0 /pseudo
(451 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17183 weakly similar to UP|Q95RC5 (Q95RC5) LD44506p (CG5745-PA... 160 4e-57
AV408566 120 5e-28
BP057230 46 1e-05
TC15464 46 1e-05
BG662230 40 8e-04
TC19635 40 8e-04
BP043290 31 0.48
TC20159 similar to GB|AAP68272.1|31711832|BT008833 At5g15880 {Ar... 30 0.62
TC14010 similar to UP|Q9SK61 (Q9SK61) Expressed protein, partial... 30 0.62
TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPas... 30 1.1
TC15953 similar to UP|B2_DAUCA (P37707) B2 protein, partial (71%) 28 4.0
BP058107 28 4.0
TC11088 27 6.9
BP043859 27 6.9
AV426390 27 6.9
BP075115 27 6.9
BP055837 27 9.0
TC8928 similar to UP|AAQ84307 (AAQ84307) Fiber protein Fb4 (Frag... 27 9.0
TC14070 homologue to UP|G3PC_PEA (P34922) Glyceraldehyde 3-phosp... 27 9.0
>TC17183 weakly similar to UP|Q95RC5 (Q95RC5) LD44506p (CG5745-PA), partial
(6%)
Length = 678
Score = 160 bits (404), Expect(2) = 4e-57
Identities = 74/86 (86%), Positives = 83/86 (96%)
Frame = +1
Query: 321 QPGIQRLVFKLKELVRRIDEPISQHIEDQGLEFLQFAFRWFNCLLIREIPFDLITRLWDT 380
QPGIQRLVFKLKELVRRID+P+S H+E+QGLEFLQFAFRWFNCLLIREIPF+++TRLWDT
Sbjct: 1 QPGIQRLVFKLKELVRRIDDPVSTHMENQGLEFLQFAFRWFNCLLIREIPFNMVTRLWDT 180
Query: 381 YLAEGDALPDFLVYIFASFLLTTNSR 406
YLAEGDALPDFLVYIFASFLLT + +
Sbjct: 181 YLAEGDALPDFLVYIFASFLLTWSDK 258
Score = 78.6 bits (192), Expect(2) = 4e-57
Identities = 37/48 (77%), Positives = 39/48 (81%)
Frame = +2
Query: 403 TNSRGWNSRSW*CSFNTFQLRTGLTRTSRWCFPGHLCGTACSTTLQAI 450
TN R S+ W*CSFNTFQLRTGLTR+SRW F HLCGTACSTTLQ I
Sbjct: 254 TNYRSLISKIW*CSFNTFQLRTGLTRSSRWYFRVHLCGTACSTTLQTI 397
>AV408566
Length = 423
Score = 120 bits (301), Expect = 5e-28
Identities = 68/121 (56%), Positives = 85/121 (70%)
Frame = +3
Query: 1 MNKPPRENHERNNNNNNTVTTLDSRFSQTLRNVQGLLKGRSMPGKVLMSRRSEPSDNLNS 60
+N+ +N++ +NNNN + DSRFSQTLR+VQGLLKGRSMPGK+L+S+R +P D NS
Sbjct: 72 INQSTMKNNDEDNNNNPNIGIFDSRFSQTLRSVQGLLKGRSMPGKILLSQRVDPID--NS 245
Query: 61 KVSSTFYKRSFSHNDAGTSDQISGAVEEEVQSTSKSVSAANVSKLKSSTSLGENLSEDIR 120
+SS Y RS S+NDAGTSD S VE EV S+S +KLK STS EN SED+R
Sbjct: 246 NLSSPTYTRSNSYNDAGTSDHASETVEVEVHSSS---GIPGENKLKISTSHVENPSEDVR 416
Query: 121 K 121
K
Sbjct: 417 K 419
>BP057230
Length = 541
Score = 46.2 bits (108), Expect = 1e-05
Identities = 27/72 (37%), Positives = 37/72 (50%), Gaps = 3/72 (4%)
Frame = -2
Query: 204 PDS--ERSDDEVNMLRQIAVDCPRTVPDV-TFFQQQQVQKSLERILYAWAIRHPASGYVQ 260
PDS R + R + D R P+ ++FQ Q L RIL W +RHP GY Q
Sbjct: 405 PDSTWSRFFRNAELERMVDQDLSRLYPEHGSYFQTPGCQGMLRRILLLWCLRHPECGYRQ 226
Query: 261 GINDLVTPFLVV 272
G+++L+ P L V
Sbjct: 225 GMHELLAPLLYV 190
>TC15464
Length = 672
Score = 45.8 bits (107), Expect = 1e-05
Identities = 29/102 (28%), Positives = 51/102 (49%), Gaps = 5/102 (4%)
Frame = +2
Query: 310 LDGMQDHYTF----AQPGIQRLVFKLKELVRRIDEPISQHIEDQGLEFLQF-AFRWFNCL 364
L G +D++ + GI+ + +L +L++ DE + +H+E QF AFRW L
Sbjct: 2 LSGFRDNFVQQLDNSPVGIRATITRLSQLLKEHDEELWRHLEITSKVNPQFYAFRWITLL 181
Query: 365 LIREIPFDLITRLWDTYLAEGDALPDFLVYIFASFLLTTNSR 406
L +E F +WDT L++ + + L+ I + L+ R
Sbjct: 182 LTQEFNFADSIHIWDTILSDPEGPQETLLRICCAMLILIRKR 307
>BG662230
Length = 340
Score = 40.0 bits (92), Expect = 8e-04
Identities = 23/67 (34%), Positives = 36/67 (53%), Gaps = 5/67 (7%)
Frame = +3
Query: 212 EVNMLRQIAVDCPRTVPDVTFFQQQQV-----QKSLERILYAWAIRHPASGYVQGINDLV 266
+ ++ QI D RT PD+ FF Q++L+ IL +A +P YVQG+N+++
Sbjct: 120 DTEIIDQIDRDVMRTHPDMHFFSGDSQFAKLNQEALKNILIIFAKLNPGVRYVQGMNEVL 299
Query: 267 TPFLVVF 273
P VF
Sbjct: 300 APLFYVF 320
>TC19635
Length = 566
Score = 40.0 bits (92), Expect = 8e-04
Identities = 23/75 (30%), Positives = 39/75 (51%), Gaps = 1/75 (1%)
Frame = +3
Query: 333 ELVRRIDEPISQHIEDQGLEFLQF-AFRWFNCLLIREIPFDLITRLWDTYLAEGDALPDF 391
+L++ DE + +H+E QF +FRW LL +E F I +WDT L++ + +
Sbjct: 3 QLLKEHDEELWRHLEVTTKVNPQFYSFRWITLLLTQEFNFHDILHIWDTLLSDPEGPQET 182
Query: 392 LVYIFASFLLTTNSR 406
L+ I + L+ R
Sbjct: 183 LLRICCAMLILVRRR 227
>BP043290
Length = 439
Score = 30.8 bits (68), Expect = 0.48
Identities = 31/95 (32%), Positives = 46/95 (47%), Gaps = 7/95 (7%)
Frame = +1
Query: 34 QGLLKGRSMPGKVLMSRRSEPSDNLNSKVSSTFYKRSFSHN-DAGTSDQI---SGAVEEE 89
Q L G+S P + S+R+ PS NL+ K + K ++ TS + S + +
Sbjct: 106 QSKLLGKSTPSSTISSKRASPS-NLHVKSENDKAKGLIGGKVNSMTSTPVFKGSQVIVPK 282
Query: 90 VQSTSKS---VSAANVSKLKSSTSLGENLSEDIRK 121
+SKS +S A +K + STS G NLS I K
Sbjct: 283 PTISSKSPSGLSGATKTKPRISTSSGSNLSGSIGK 387
>TC20159 similar to GB|AAP68272.1|31711832|BT008833 At5g15880 {Arabidopsis
thaliana;}, partial (36%)
Length = 655
Score = 30.4 bits (67), Expect = 0.62
Identities = 26/87 (29%), Positives = 44/87 (49%), Gaps = 2/87 (2%)
Frame = +1
Query: 18 TVTTLDSRFSQTLRNVQGLLKGRSMPGKVLMSRRSEPSDNLNSKVSST--FYKRSFSHND 75
T+ ++ F + N + +K + + K L+ RR E D K+ + ++ + D
Sbjct: 370 TLKEENAAFENAISNCENKIKEK-LQEKDLLQRRLEEMDETEKKLRTDQDSVQQLQASQD 546
Query: 76 AGTSDQISGAVEEEVQSTSKSVSAANV 102
AG S SGA EEE ++ SK+ S A+V
Sbjct: 547 AGKSWN-SGAWEEETKTNSKAGSDADV 624
>TC14010 similar to UP|Q9SK61 (Q9SK61) Expressed protein, partial (53%)
Length = 730
Score = 30.4 bits (67), Expect = 0.62
Identities = 19/77 (24%), Positives = 35/77 (44%), Gaps = 4/77 (5%)
Frame = +1
Query: 2 NKPPRENHERNNNNNNTVTTLDSRFSQTLRNVQGLLKGRSMP----GKVLMSRRSEPSDN 57
+KP R N+ R+ N N T+ ++ L + ++GRS P G++ + + D
Sbjct: 67 SKPMRANNSRSGNYRNPCLTMHQPWASLLVHGIKRIEGRSWPAPITGRLWIHAAGKVPDE 246
Query: 58 LNSKVSSTFYKRSFSHN 74
+ FYK ++ N
Sbjct: 247 STIQAMEYFYKEIYALN 297
>TC10924 homologue to UP|Q8H9D4 (Q8H9D4) 26S proteasome AAA-ATPase subunit
RPT4a, partial (49%)
Length = 648
Score = 29.6 bits (65), Expect = 1.1
Identities = 29/110 (26%), Positives = 50/110 (45%), Gaps = 15/110 (13%)
Frame = +2
Query: 85 AVEEEVQSTSKSVSAANVSKLKSSTSLGENLSEDIR---------------KYTIGARAT 129
+V E +Q++ K + LKS S+G+ + E +R +Y +G R+
Sbjct: 143 SVRENLQASKKEYNKTE-DDLKSLQSVGQIIGEVLRPLDNERLIVKASSGPRYVVGCRS- 316
Query: 130 DSARVTKFNKVLSGTVVILDNLRELAWSGVPDYMRPKVWRLLLGYEPPNS 179
+V K K+ SGT V+LD +P + P V+ +L +E P +
Sbjct: 317 ---KVDK-EKLTSGTRVVLDMTTLTIMRALPREVDPVVYNML--HEDPGN 448
>TC15953 similar to UP|B2_DAUCA (P37707) B2 protein, partial (71%)
Length = 919
Score = 27.7 bits (60), Expect = 4.0
Identities = 15/62 (24%), Positives = 32/62 (51%), Gaps = 3/62 (4%)
Frame = +1
Query: 4 PPRENHERNNNNNNTVT---TLDSRFSQTLRNVQGLLKGRSMPGKVLMSRRSEPSDNLNS 60
P ++ E NNNNNN + + D++ +TL + L + ++ G + + ++NL
Sbjct: 82 PNKKQGESNNNNNNNDSNNKSKDAKKFKTLPASESLPRNETIGGYIFVCNNDTMAENLKR 261
Query: 61 KV 62
++
Sbjct: 262 QL 267
>BP058107
Length = 496
Score = 27.7 bits (60), Expect = 4.0
Identities = 16/60 (26%), Positives = 28/60 (46%)
Frame = +3
Query: 55 SDNLNSKVSSTFYKRSFSHNDAGTSDQISGAVEEEVQSTSKSVSAANVSKLKSSTSLGEN 114
S + +S ++T + + S + A TS +G+ STS + + S + SLG N
Sbjct: 132 SGSTSSTSTNTLSRITLSTSSASTSSASTGSASTSSASTSSASRSCGSSNSRKGNSLGTN 311
>TC11088
Length = 534
Score = 26.9 bits (58), Expect = 6.9
Identities = 10/18 (55%), Positives = 15/18 (82%)
Frame = -2
Query: 262 INDLVTPFLVVFISEHLE 279
+NDL+T L VF+SEH++
Sbjct: 395 LNDLLTNHLTVFLSEHIQ 342
>BP043859
Length = 551
Score = 26.9 bits (58), Expect = 6.9
Identities = 18/67 (26%), Positives = 30/67 (43%)
Frame = +1
Query: 12 NNNNNNTVTTLDSRFSQTLRNVQGLLKGRSMPGKVLMSRRSEPSDNLNSKVSSTFYKRSF 71
NNNNNN +R S + L + R + + + R S D + V++ ++S
Sbjct: 49 NNNNNNDAIDFTARNSSLTESFYALSRFRIIHHSIRIVRSSLQIDETSVAVTTKPPQKSA 228
Query: 72 SHNDAGT 78
+H A T
Sbjct: 229 THAHAHT 249
>AV426390
Length = 399
Score = 26.9 bits (58), Expect = 6.9
Identities = 16/49 (32%), Positives = 22/49 (44%), Gaps = 3/49 (6%)
Frame = +1
Query: 4 PPRENHERNNNNNNTV---TTLDSRFSQTLRNVQGLLKGRSMPGKVLMS 49
P N+ NNNNNNT+ L S + TL + G + +L S
Sbjct: 64 PTNSNNHNNNNNNNTIPIPIALPSSLTPTLLPSPTSVTGNLITPPILFS 210
>BP075115
Length = 413
Score = 26.9 bits (58), Expect = 6.9
Identities = 10/18 (55%), Positives = 15/18 (82%)
Frame = +3
Query: 262 INDLVTPFLVVFISEHLE 279
+NDL+T L VF+SEH++
Sbjct: 195 LNDLLTNHLTVFLSEHIQ 248
>BP055837
Length = 528
Score = 26.6 bits (57), Expect = 9.0
Identities = 26/94 (27%), Positives = 42/94 (44%)
Frame = -1
Query: 210 DDEVNMLRQIAVDCPRTVPDVTFFQQQQVQKSLERILYAWAIRHPASGYVQGINDLVTPF 269
++E+ L QIAV C VPD + V K +E + P V +++ +
Sbjct: 291 EEEMVALLQIAVACTAAVPDQR-PRMSHVVKMIEELRGVQV--SPCHDTVDSVSESPS-- 127
Query: 270 LVVFISEHLEGGIDDWSMSDLSSDKISNVEADCY 303
+SE GG + S+S L +D S ++ CY
Sbjct: 126 ----LSEDACGGGTN*SVSKLGNDLCSACKSICY 37
>TC8928 similar to UP|AAQ84307 (AAQ84307) Fiber protein Fb4 (Fragment),
partial (72%)
Length = 1278
Score = 26.6 bits (57), Expect = 9.0
Identities = 67/328 (20%), Positives = 120/328 (36%), Gaps = 5/328 (1%)
Frame = +1
Query: 12 NNNNNNTVTTLDSRFSQTLRNVQGLLKGRSMPGKVLMSRRSEPSDNLNSKVSSTFYKRSF 71
+NNN+NT ++L +F Q G P L ++P D + +K + + + F
Sbjct: 394 SNNNDNTPSSLHVQFIQC---------GLRTPQCTLQGTLTKPQDQIKAKRLVSLWGKRF 546
Query: 72 SHNDAGTSDQISGAVEEEVQSTSKSVSAANVSKLKSSTSLGENLSEDIRKYTIGARATDS 131
S I AV+ + S V+ L + + L E + +
Sbjct: 547 GEEVDEDSVYII-AVDRVLHLDDFQESGVWVTSLDYKNAQPDPLREFAEELVTEINTNNM 723
Query: 132 ARVTKFNKVLSGTVVILDNLRELAWSGVPDYMRPKVWRLLLGYEPPNSDRKEGVLGRKRG 191
VT+F V V LD P VW LG++ S +G +
Sbjct: 724 EDVTRFCNV----YVDLD---------FPVSQAKMVWVDRLGFDMRLSCPLQGTFEAR-- 858
Query: 192 EYLDCISQYYDIPDSERSDDEVNMLRQIAVDCPRTV--PDVTFFQQQQVQKS--LERILY 247
I ++ D + + N + Q+A + + PD +Q + KS +L+
Sbjct: 859 -----IPFPREVTDEKGAKSSFNCMSQLAWEVEKNFHPPDFQKVKQLKPVKS*CKSCLLF 1023
Query: 248 AWAIRHPASGYVQGIN-DLVTPFLVVFISEHLEGGIDDWSMSDLSSDKISNVEADCYWCL 306
++I + V+ N ++P L+ S + G+D + D + +V++
Sbjct: 1024LFSIVVKYASLVEPCNSSRISPCLLCLYSVMIRLGLDV*IIVKTEHDVVLHVKS------ 1185
Query: 307 SKLLDGMQDHYTFAQPGIQRLVFKLKEL 334
+ F PG+ L F LKE+
Sbjct: 1186----------WPFV*PGLCILNFLLKEM 1239
>TC14070 homologue to UP|G3PC_PEA (P34922) Glyceraldehyde 3-phosphate
dehydrogenase, cytosolic , complete
Length = 1414
Score = 26.6 bits (57), Expect = 9.0
Identities = 13/41 (31%), Positives = 21/41 (50%)
Frame = +2
Query: 76 AGTSDQISGAVEEEVQSTSKSVSAANVSKLKSSTSLGENLS 116
A T DQI A++EE + K + + S+ +G+N S
Sbjct: 809 AATYDQIKAAIKEESEGKLKGILGYTEDDVVSTDFIGDNRS 931
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.320 0.135 0.411
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,832,365
Number of Sequences: 28460
Number of extensions: 105607
Number of successful extensions: 691
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 632
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 655
length of query: 451
length of database: 4,897,600
effective HSP length: 93
effective length of query: 358
effective length of database: 2,250,820
effective search space: 805793560
effective search space used: 805793560
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 57 (26.6 bits)
Medicago: description of AC137825.12