BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137702.2 + phase: 0
(257 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC17523 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alph... 253 2e-68
TC11925 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alph... 211 1e-55
TC9187 similar to UP|Q8LAN3 (Q8LAN3) Prolyl 4-hydroxylase alpha ... 205 5e-54
TC15863 199 3e-52
TC12024 160 2e-40
TC13277 similar to UP|Q9LSI6 (Q9LSI6) Prolyl 4-hydroxylase alpha... 138 1e-33
AV423632 95 1e-20
BP069281 84 3e-17
AV415701 70 5e-13
TC17036 similar to UP|Q8VZD7 (Q8VZD7) AT3g28480/MFJ20_16, partia... 64 2e-11
TC15896 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, ... 64 2e-11
AV766573 53 6e-08
TC16912 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, ... 52 1e-07
AW720438 43 6e-05
TC19415 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, ... 37 0.004
BP061999 34 0.022
TC16820 similar to UP|Q8L8T9 (Q8L8T9) Prolyl 4-hydroxylase alpha... 32 0.083
TC13069 27 2.7
AW719680 27 3.5
TC11371 similar to UP|Q8S9K1 (Q8S9K1) At1g23880/T23E23_8, partia... 26 7.7
>TC17523 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alpha
subunit-like protein, partial (55%)
Length = 448
Score = 253 bits (646), Expect = 2e-68
Identities = 116/147 (78%), Positives = 134/147 (90%)
Frame = +1
Query: 103 KSSVVDSKTGKSTESRVRTSSGMFLKRGKDKIIQNIERRIADFTFIPVENGEGLQVLHYG 162
+S+VVDS+TGKS +SRVRTSSG FL RG+DKI++NIE++IADFTFIPVE+GEGLQVLHY
Sbjct: 7 RSTVVDSETGKSKDSRVRTSSGTFLPRGRDKIVRNIEKKIADFTFIPVEHGEGLQVLHYE 186
Query: 163 VGEKYEPHYDYFLDEFNTKNGGQRVATVLMYLSDVEEGGETVFPAAKANFSSVPWWNDLS 222
VG+KYEPHYDYFLDEFNTKNGGQR+ATVLMYL+DVE GGETVFP+AK NF SVPWWN+LS
Sbjct: 187 VGQKYEPHYDYFLDEFNTKNGGQRIATVLMYLTDVEXGGETVFPSAKGNFXSVPWWNELS 366
Query: 223 ECARKGLSLKPKMGDALLFWSMRPDAT 249
+C +KGLS+ P GDALLFWSM P AT
Sbjct: 367 DCGKKGLSINPXXGDALLFWSMNPXAT 447
>TC11925 similar to UP|Q9FKX6 (Q9FKX6) Prolyl 4-hydroxylase, alpha
subunit-like protein, partial (43%)
Length = 613
Score = 211 bits (536), Expect = 1e-55
Identities = 105/193 (54%), Positives = 142/193 (73%), Gaps = 13/193 (6%)
Frame = +1
Query: 1 MAKGKHTHPRSQVKK-LSTLILLTLFM-LTLVIIVLLALGILYLPNTTDDSLITDRRKIY 58
M K +H+ S+ +S + LTLF+ ++++LL LG+L PN + DS + +
Sbjct: 37 MVKLRHSRMVSRKSSPMSWTLTLTLFVSFIFLVLILLILGVLSFPNASSDSGVPKPNDL- 213
Query: 59 ESLAEK-----------KEQWTEILSWEPRAFVYHNFLSKEECEHLINLAKPFLAKSSVV 107
S+A +E+W E++SWEPRAF+YHNFL+KEECE+LIN+AKP + KS+VV
Sbjct: 214 NSIARNAIRNEGDDNGPREKWVELISWEPRAFLYHNFLTKEECEYLINIAKPHMRKSTVV 393
Query: 108 DSKTGKSTESRVRTSSGMFLKRGKDKIIQNIERRIADFTFIPVENGEGLQVLHYGVGEKY 167
DS+TGKS +SR RTSSG FL RG+DKI++NIE+RIAD+TFIP+E+GEGLQVLHY VG+KY
Sbjct: 394 DSETGKSKDSRARTSSGTFLARGRDKIVRNIEKRIADYTFIPIEHGEGLQVLHYEVGQKY 573
Query: 168 EPHYDYFLDEFNT 180
+ HYDYF+DEFNT
Sbjct: 574 DSHYDYFMDEFNT 612
>TC9187 similar to UP|Q8LAN3 (Q8LAN3) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (89%)
Length = 1198
Score = 205 bits (522), Expect = 5e-54
Identities = 102/187 (54%), Positives = 136/187 (72%), Gaps = 2/187 (1%)
Frame = +2
Query: 72 LSWEPRAFVYHNFLSKEECEHLINLAKPFLAKSSVVDSKTGKSTESRVRTSSGMFLKRGK 131
+SW+PRAFVY FL+ EC+HLI+LAK L +S+V D+ +G S S VRTSSGMF+ + K
Sbjct: 179 VSWKPRAFVYEGFLTGLECDHLISLAKSELKRSAVADNLSGDSKLSEVRTSSGMFISKKK 358
Query: 132 DKIIQNIERRIADFTFIPVENGEGLQVLHYGVGEKYEPHYDYFLDEFNTKNGGQRVATVL 191
D I+ IE +I+ +TF+P ENGE +QVL Y G+KY+PHYDYF D+ N GG R+ATVL
Sbjct: 359 DPIVAGIEDKISAWTFLPKENGEDMQVLRYEHGQKYDPHYDYFTDKVNIVRGGHRMATVL 538
Query: 192 MYLSDVEEGGETVFPAAK--ANFSSVPWWNDLSECARKGLSLKPKMGDALLFWSMRPDAT 249
+YL++V GGETVFP A+ + +DLSECA+KG+++KP+ GDALLF+S+ AT
Sbjct: 539 LYLTNVTRGGETVFPVAEEPPRRRGLETNSDLSECAKKGIAVKPRRGDALLFFSLHTTAT 718
Query: 250 LDASSLH 256
D SLH
Sbjct: 719 PDPDSLH 739
>TC15863
Length = 607
Score = 199 bits (507), Expect = 3e-52
Identities = 92/103 (89%), Positives = 99/103 (95%)
Frame = +2
Query: 155 GLQVLHYGVGEKYEPHYDYFLDEFNTKNGGQRVATVLMYLSDVEEGGETVFPAAKANFSS 214
GLQVLHY VG+KYEPHYDYFLDEFNTKNGGQR+ATVLMYLSDVEEGGET+FPAAKANFSS
Sbjct: 2 GLQVLHYEVGQKYEPHYDYFLDEFNTKNGGQRIATVLMYLSDVEEGGETIFPAAKANFSS 181
Query: 215 VPWWNDLSECARKGLSLKPKMGDALLFWSMRPDATLDASSLHG 257
VPW+NDLS CA+KGLS+KPK GDALLFWS+RPDATLD SSLHG
Sbjct: 182 VPWYNDLSVCAKKGLSVKPKRGDALLFWSIRPDATLDPSSLHG 310
>TC12024
Length = 469
Score = 160 bits (406), Expect = 2e-40
Identities = 84/120 (70%), Positives = 99/120 (82%), Gaps = 6/120 (5%)
Frame = +1
Query: 1 MAKGKHTHPRSQVKKLSTLILLT--LFMLTLVIIVLLALGILYLPNTTDDSLITD----R 54
MAKGK TH RSQ K+ ST+ L+ LFMLTLVI++LLALGI+YLP T DDS ITD R
Sbjct: 109 MAKGKFTHTRSQGKRWSTVTLVASMLFMLTLVILLLLALGIVYLPTTDDDSPITDLSSFR 288
Query: 55 RKIYESLAEKKEQWTEILSWEPRAFVYHNFLSKEECEHLINLAKPFLAKSSVVDSKTGKS 114
R+ +SL E +EQWTEILSWEPRAF++HNFLSK ECE++INLAKP +AKSSVVDS+TGKS
Sbjct: 289 RRTSQSLEENQEQWTEILSWEPRAFIFHNFLSKVECEYMINLAKPHMAKSSVVDSQTGKS 468
>TC13277 similar to UP|Q9LSI6 (Q9LSI6) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (34%)
Length = 582
Score = 138 bits (347), Expect = 1e-33
Identities = 61/102 (59%), Positives = 82/102 (79%)
Frame = +1
Query: 72 LSWEPRAFVYHNFLSKEECEHLINLAKPFLAKSSVVDSKTGKSTESRVRTSSGMFLKRGK 131
LSW PRAF++ FLS +EC+HL+NLAK L S V D+++GKS +S VRTSSGMFL + +
Sbjct: 277 LSWSPRAFLHTGFLSDKECDHLVNLAKDKLEVSMVADNESGKSIKSEVRTSSGMFLNKAQ 456
Query: 132 DKIIQNIERRIADFTFIPVENGEGLQVLHYGVGEKYEPHYDY 173
D+++ +IE RIA +TF+P+ENGE +Q+LHY G+KYEPHYDY
Sbjct: 457 DEVVADIEARIATWTFLPIENGESIQILHYENGQKYEPHYDY 582
>AV423632
Length = 484
Score = 94.7 bits (234), Expect = 1e-20
Identities = 50/121 (41%), Positives = 78/121 (64%), Gaps = 11/121 (9%)
Frame = +2
Query: 1 MAKGKHTHPRSQVKKLSTLILLTLFM-LTLVIIVLLALGILYLPNTTDDSLI---TDRRK 56
MAK +++ S+ S+ ++ LF+ T ++++L ALGIL +P+++ D
Sbjct: 122 MAKPRYSRLPSRKSSSSSTLIFALFLAFTFLLLILFALGILSIPSSSSRDKFPKPNDLTS 301
Query: 57 IYESLAEKK-------EQWTEILSWEPRAFVYHNFLSKEECEHLINLAKPFLAKSSVVDS 109
I + ++ EQW E++SWEPRAFVYHNFL+KEECE+LI++AKP + KS+VVDS
Sbjct: 302 IAHNTLDRTDDDDGRGEQWVEVISWEPRAFVYHNFLTKEECEYLIDIAKPNMHKSTVVDS 481
Query: 110 K 110
+
Sbjct: 482 E 484
>BP069281
Length = 468
Score = 83.6 bits (205), Expect = 3e-17
Identities = 34/45 (75%), Positives = 40/45 (88%)
Frame = -3
Query: 213 SSVPWWNDLSECARKGLSLKPKMGDALLFWSMRPDATLDASSLHG 257
S+VPWWN+LS+C +KGLS+KPK GDALLFWSM+PD T D SSLHG
Sbjct: 466 SAVPWWNELSDCGKKGLSIKPKRGDALLFWSMKPDTTADTSSLHG 332
>AV415701
Length = 418
Score = 69.7 bits (169), Expect = 5e-13
Identities = 38/55 (69%), Positives = 44/55 (79%), Gaps = 2/55 (3%)
Frame = +1
Query: 1 MAKGKHTHPRSQVKKLSTLILLT--LFMLTLVIIVLLALGILYLPNTTDDSLITD 53
MAKGK TH RSQ K+ ST+ L+ LFMLTLVI++LLALGI+YLP T DDS ITD
Sbjct: 226 MAKGKFTHTRSQGKRWSTVTLVASMLFMLTLVILLLLALGIVYLPTTDDDSPITD 390
>TC17036 similar to UP|Q8VZD7 (Q8VZD7) AT3g28480/MFJ20_16, partial (21%)
Length = 697
Score = 64.3 bits (155), Expect = 2e-11
Identities = 32/61 (52%), Positives = 44/61 (71%)
Frame = +2
Query: 197 VEEGGETVFPAAKANFSSVPWWNDLSECARKGLSLKPKMGDALLFWSMRPDATLDASSLH 256
V +GGET+FP A++ S P SECA KG ++KP+ GDALLF+S+ +AT D++SLH
Sbjct: 2 VGKGGETIFPHAESKLSQ-PKDESWSECAHKGYAVKPRKGDALLFFSLHLNATTDSNSLH 178
Query: 257 G 257
G
Sbjct: 179 G 181
>TC15896 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, partial
(45%)
Length = 613
Score = 64.3 bits (155), Expect = 2e-11
Identities = 38/94 (40%), Positives = 57/94 (60%), Gaps = 4/94 (4%)
Frame = +3
Query: 70 EILSWEPRAFVYHNFLSKEECEHLINLAKPFLAKSSVVDSKTGKSTESR--VRTSSGMFL 127
++LSW P A + NF + E+CE +I AK L S++V + G++ ES +RTSSG+F+
Sbjct: 333 QVLSWNPHALYFPNFATAEQCESIIETAKEGLKPSTLV-LRVGETDESTTGIRTSSGVFI 509
Query: 128 KRGKDK--IIQNIERRIADFTFIPVENGEGLQVL 159
+DK ++ IE +IA T IP +GE VL
Sbjct: 510 SAFEDKTGVLDVIEEKIARATKIPRTHGEAFNVL 611
>AV766573
Length = 338
Score = 52.8 bits (125), Expect = 6e-08
Identities = 23/27 (85%), Positives = 25/27 (92%)
Frame = -3
Query: 231 LKPKMGDALLFWSMRPDATLDASSLHG 257
+KPK GDALLFWS+RPDATLD SSLHG
Sbjct: 336 VKPKRGDALLFWSIRPDATLDPSSLHG 256
>TC16912 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, partial
(30%)
Length = 518
Score = 51.6 bits (122), Expect = 1e-07
Identities = 24/66 (36%), Positives = 45/66 (67%), Gaps = 2/66 (3%)
Frame = +1
Query: 70 EILSWEPRAFVYHNFLSKEECEHLINLAKPFLAKSSVV--DSKTGKSTESRVRTSSGMFL 127
++LSW+PRA + NF + E+C+ ++ +AK L S++ + +T ++T+ +RTSSG+F+
Sbjct: 295 QVLSWKPRALYFPNFATAEQCKSIVGVAKAGLKPSALALREGETEQNTKG-IRTSSGVFV 471
Query: 128 KRGKDK 133
+DK
Sbjct: 472 SSSEDK 489
>AW720438
Length = 463
Score = 42.7 bits (99), Expect = 6e-05
Identities = 18/34 (52%), Positives = 25/34 (72%)
Frame = +3
Query: 72 LSWEPRAFVYHNFLSKEECEHLINLAKPFLAKSS 105
+SW+PR F+Y FLS +EC++LI+LA KSS
Sbjct: 357 ISWQPRVFLYKGFLSDKECDYLISLAYAVKEKSS 458
>TC19415 similar to UP|BAD07294 (BAD07294) Prolyl 4-hydroxylase, partial
(24%)
Length = 443
Score = 36.6 bits (83), Expect = 0.004
Identities = 15/30 (50%), Positives = 23/30 (76%)
Frame = +3
Query: 228 GLSLKPKMGDALLFWSMRPDATLDASSLHG 257
GL ++P+ GD LLF+S+ P+ T+D +SL G
Sbjct: 51 GLRVRPRPGDGLLFYSLFPNGTIDPTSLPG 140
>BP061999
Length = 564
Score = 34.3 bits (77), Expect = 0.022
Identities = 13/17 (76%), Positives = 14/17 (81%)
Frame = -2
Query: 241 FWSMRPDATLDASSLHG 257
FWSM+PD T D SSLHG
Sbjct: 563 FWSMKPDTTADTSSLHG 513
>TC16820 similar to UP|Q8L8T9 (Q8L8T9) Prolyl 4-hydroxylase alpha
subunit-like protein, partial (7%)
Length = 571
Score = 32.3 bits (72), Expect = 0.083
Identities = 14/25 (56%), Positives = 18/25 (72%)
Frame = +2
Query: 227 KGLSLKPKMGDALLFWSMRPDATLD 251
+GLS+KP G+A+LFWSM D D
Sbjct: 287 EGLSVKPTKGNAVLFWSMGLDGQSD 361
>TC13069
Length = 450
Score = 27.3 bits (59), Expect = 2.7
Identities = 14/30 (46%), Positives = 16/30 (52%)
Frame = -3
Query: 72 LSWEPRAFVYHNFLSKEECEHLINLAKPFL 101
L W R VYH +SK C HLI + FL
Sbjct: 94 LLWNKRMIVYHQTVSKNGC-HLIIIFNNFL 8
>AW719680
Length = 525
Score = 26.9 bits (58), Expect = 3.5
Identities = 7/17 (41%), Positives = 14/17 (82%)
Frame = +3
Query: 70 EILSWEPRAFVYHNFLS 86
+++SW R F++HNF++
Sbjct: 357 QLISWHTRRFIWHNFIN 407
>TC11371 similar to UP|Q8S9K1 (Q8S9K1) At1g23880/T23E23_8, partial (41%)
Length = 1023
Score = 25.8 bits (55), Expect = 7.7
Identities = 15/41 (36%), Positives = 24/41 (57%)
Frame = +2
Query: 12 QVKKLSTLILLTLFMLTLVIIVLLALGILYLPNTTDDSLIT 52
QVKKL+ L+ L FML V++ L + L ++ +L+T
Sbjct: 686 QVKKLNFLMTLMWFMLEAVVLYSL*IEETELSERSNSTLMT 808
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.135 0.399
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,333,560
Number of Sequences: 28460
Number of extensions: 53510
Number of successful extensions: 305
Number of sequences better than 10.0: 42
Number of HSP's better than 10.0 without gapping: 296
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 296
length of query: 257
length of database: 4,897,600
effective HSP length: 88
effective length of query: 169
effective length of database: 2,393,120
effective search space: 404437280
effective search space used: 404437280
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC137702.2