BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137603.8 - phase: 0 /pseudo
(676 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BP065754 50 9e-07
TC11175 weakly similar to GB|AAL06796.1|15809736|AY054135 AT3g60... 31 0.75
TC15216 similar to UP|O64851 (O64851) At2g26190 protein (At2g261... 30 1.3
TC17411 similar to UP|Q8LDG6 (Q8LDG6) Protein kinase, partial (33%) 29 2.2
TC11531 similar to AAQ56832 (AAQ56832) At2g34040, partial (32%) 29 2.2
BP069580 29 2.8
BP036724 28 4.8
BI419832 28 4.8
TC11843 28 6.3
BP067822 28 6.3
TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-bi... 27 8.2
>BP065754
Length = 319
Score = 50.4 bits (119), Expect = 9e-07
Identities = 27/66 (40%), Positives = 42/66 (62%), Gaps = 3/66 (4%)
Frame = +2
Query: 41 VPSSLV---EIAPILRVANEVEKTHPRVAYLCRFYAFEKAHRLDPTSSGRGVRQFKTALL 97
VPS+L +I ILRVA+E++ P V+ + +A+ + LDP S GRGV QFKT L+
Sbjct: 119 VPSALAKNRDIDEILRVADEIQDDDPTVSRILCEHAYSLSQNLDPNSEGRGVLQFKTGLM 298
Query: 98 QRLERE 103
++++
Sbjct: 299 SVIKQK 316
>TC11175 weakly similar to GB|AAL06796.1|15809736|AY054135
AT3g60690/T4C21_100 {Arabidopsis thaliana;}, partial
(42%)
Length = 692
Score = 30.8 bits (68), Expect = 0.75
Identities = 19/63 (30%), Positives = 28/63 (44%)
Frame = +1
Query: 333 LLIWGEAANLRFMPECLCYIYHHMAFELYGMLAGNVSPMTGENIKPAYGGEEEAFLRKVV 392
LL WG L L + HH + Y L N+ P + P Y GEE+ L +++
Sbjct: 34 LLKWGR--KLTACATSLLTVRHHGSG--YAQLGSNLDPKVPKGHLPVYVGEEDGELHRLL 201
Query: 393 TPI 395
P+
Sbjct: 202 VPV 210
>TC15216 similar to UP|O64851 (O64851) At2g26190 protein
(At2g26190/T1D16.17), partial (39%)
Length = 1255
Score = 30.0 bits (66), Expect = 1.3
Identities = 19/52 (36%), Positives = 26/52 (49%), Gaps = 2/52 (3%)
Frame = +3
Query: 271 IRQFPKPDQQPKLDERALTEVMKKLFKNYKKW--CKYLGRKSSLWLPTIQQE 320
I Q KP+ KL+E +LT+ +KKL KW + GR LW + E
Sbjct: 807 IAQPKKPNSSNKLNETSLTKKIKKLHS*P*KWKLWSWQGRGHFLWRQQVNDE 962
>TC17411 similar to UP|Q8LDG6 (Q8LDG6) Protein kinase, partial (33%)
Length = 632
Score = 29.3 bits (64), Expect = 2.2
Identities = 15/55 (27%), Positives = 28/55 (50%)
Frame = +2
Query: 105 DPTLKGRIKKSDAREMQSFYQHYYKKYIQALQNAADKADRAQLTKAYQTANVLFE 159
D ++ + + A + F +++YK Y+Q LQ+ D+ R L + Q + V E
Sbjct: 152 DVSVSSPVTRQKAAAAKQFIENHYKNYLQGLQDRKDR--RRALQRKVQESQVSAE 310
>TC11531 similar to AAQ56832 (AAQ56832) At2g34040, partial (32%)
Length = 535
Score = 29.3 bits (64), Expect = 2.2
Identities = 16/53 (30%), Positives = 25/53 (46%), Gaps = 9/53 (16%)
Frame = +1
Query: 337 GEAANLRFMPECLCYIYHHMAFEL---------YGMLAGNVSPMTGENIKPAY 380
GE N ++ ECL Y +HH+A ++ Y ++ G S GE+ Y
Sbjct: 310 GEEMNFTYV-ECLLYAFHHLAHKVPNATNSLCGYKIVTGQPSDRLGEDFSEQY 465
>BP069580
Length = 483
Score = 28.9 bits (63), Expect = 2.8
Identities = 19/56 (33%), Positives = 30/56 (52%), Gaps = 2/56 (3%)
Frame = -2
Query: 291 VMKKLFKNYKKWCK--YLGRKSSLWLPTIQQEVQQRKLLYMGLYLLIWGEAANLRF 344
+++ F WCK +L SS+ + +Q++V RKL++ LLIWG L F
Sbjct: 338 IVEVFFNKQALWCK*HFLFTMSSI-IQELQEDVVCRKLVH----LLIWGHTI*LEF 186
>BP036724
Length = 499
Score = 28.1 bits (61), Expect = 4.8
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = +2
Query: 602 HSPSMFILAVVVYLSPNMLAAILFLLYIDSNLVVLFPCT*QPRL 645
H P +F+L V YLS N++ A F + S+ + + PRL
Sbjct: 338 HGPLVFMLIAVSYLSSNVVLAAEFTITSTSSAITSDRSSSSPRL 469
>BI419832
Length = 543
Score = 28.1 bits (61), Expect = 4.8
Identities = 12/34 (35%), Positives = 19/34 (55%)
Frame = +2
Query: 577 VTYAYTWDNPPGFAQSIQSWFGSNSHSPSMFILA 610
+T + + PP +AQS+ S+ S+ S F LA
Sbjct: 95 ITSQHNFSAPPSYAQSLPSFSASSGQMASQFFLA 196
>TC11843
Length = 452
Score = 27.7 bits (60), Expect = 6.3
Identities = 13/54 (24%), Positives = 30/54 (55%)
Frame = -1
Query: 543 VILNWKAQRSMSMHAKLRYILKVVSGAAWVIVLSVTYAYTWDNPPGFAQSIQSW 596
++L W+ R+ +H++L+YI K ++ +++ T++ PPG + S+
Sbjct: 230 ILLEWRIGRARFVHSRLKYISK*LNS----VIIL*TWSSRIRMPPGCVSYLSSF 81
>BP067822
Length = 383
Score = 27.7 bits (60), Expect = 6.3
Identities = 14/42 (33%), Positives = 24/42 (56%), Gaps = 4/42 (9%)
Frame = +3
Query: 296 FKNYKKWCKYLGRKSSLWL----PTIQQEVQQRKLLYMGLYL 333
FKN +K+ K+ ++SLWL PT + Q+ Y+ +Y+
Sbjct: 258 FKNNQKYLKHASPQTSLWLKCDRPTSSKGPNQKAHKYIYIYM 383
>TC7956 similar to GB|AAM61419.1|21537078|AY084855 putaive DNA-binding
protein {Arabidopsis thaliana;} , partial (45%)
Length = 1257
Score = 27.3 bits (59), Expect = 8.2
Identities = 15/43 (34%), Positives = 22/43 (50%)
Frame = -3
Query: 3 SRPGPPSSSETQGPPLQRRITRTQTAGNLGEAIFDSEVVPSSL 45
S+PGPP S +GP L +T G A FD + P+++
Sbjct: 961 SQPGPPLPSRNEGPTLTVPMTEFPKR-RRGSAQFDRRLKPTAV 836
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.323 0.137 0.422
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,819,658
Number of Sequences: 28460
Number of extensions: 168818
Number of successful extensions: 862
Number of sequences better than 10.0: 22
Number of HSP's better than 10.0 without gapping: 862
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 862
length of query: 676
length of database: 4,897,600
effective HSP length: 96
effective length of query: 580
effective length of database: 2,165,440
effective search space: 1255955200
effective search space used: 1255955200
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 58 (26.9 bits)
Medicago: description of AC137603.8