BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137553.2 - phase: 0
(264 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AU251673 58 1e-09
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 49 7e-07
BI418821 49 9e-07
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 47 3e-06
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 43 6e-05
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 42 8e-05
TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 37 0.005
TC17929 31 0.19
AV421754 29 0.73
AV420911 28 1.6
TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, ... 28 1.6
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 28 1.6
TC12591 28 2.1
TC10525 homologue to UP|AAB94924 (AAB94924) Histone H4, complete 27 2.8
TC18038 similar to GB|CAC04249.1|9886727|PSA297606 PPF-1 protein... 27 3.6
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 27 4.7
BP036788 27 4.7
BP079571 23 5.3
BP039068 26 6.2
TC18767 26 6.2
>AU251673
Length = 413
Score = 58.2 bits (139), Expect = 1e-09
Identities = 36/121 (29%), Positives = 62/121 (50%), Gaps = 1/121 (0%)
Frame = +2
Query: 6 ADDWWKSLLPVLEQDGVVVTWAVFRREFLNRYFPEDVRGKKEIEFLELKQGD-MSVTEYA 64
A DW+ L TWA F EF+NR+ P+ VR +F L+Q + M+V+EY+
Sbjct: 56 ARDWYNVLTRAKPVGSPPWTWADFSAEFMNRFLPQSVRDGFVRDFERLEQAEGMTVSEYS 235
Query: 65 AKFVELAKFYPHYSAETAEFSKCIKFENGLRADIKRAIGYQKIRNFSELVSSYMIYEEDT 124
A F L+++ P+ E + +F GL+ + +++ K SE++S ++ E+
Sbjct: 236 AHFTHLSRYVPY---PLLEEERVKRFVRGLKEYLFKSVVGSKSSTLSEVLSLALLVEQRQ 406
Query: 125 K 125
K
Sbjct: 407 K 409
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 49.3 bits (116), Expect = 7e-07
Identities = 33/103 (32%), Positives = 44/103 (42%), Gaps = 6/103 (5%)
Frame = -2
Query: 135 RNKGQQGRPKPYSAPVNKGEQRL------NDERRPKRRDDPAEIVCYKCGEKGHKSNVCN 188
RNK Q KP+ P N+G N RP + D +EIVC++C +KGH +N C
Sbjct: 455 RNKSFQ--KKPFQRPQNRGTSSGYSHSFGNFVPRPTQ-SDTSEIVCHRCSKKGHFANRCP 285
Query: 189 GEEKKCFRCGKKWHTIAECKRGDIVCFNCDEEGHLTSQCKKPK 231
D+VC+NC + GH C PK
Sbjct: 284 ----------------------DLVCWNCQKTGHSGKDCTNPK 222
>BI418821
Length = 614
Score = 48.9 bits (115), Expect = 9e-07
Identities = 22/71 (30%), Positives = 30/71 (41%), Gaps = 15/71 (21%)
Frame = +2
Query: 174 CYKCGEKGHKSNVCN--------GEEKKCFRCGKKWHTIAECKR-------GDIVCFNCD 218
CY CG+ GH + C+ G C+ CG H +C R G C+NC
Sbjct: 392 CYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGGGAGCYNCG 571
Query: 219 EEGHLTSQCKK 229
+ GHL C +
Sbjct: 572 DTGHLARDCNR 604
Score = 36.6 bits (83), Expect = 0.005
Identities = 15/45 (33%), Positives = 20/45 (44%), Gaps = 7/45 (15%)
Frame = +2
Query: 174 CYKCGEKGHKSNVCN-------GEEKKCFRCGKKWHTIAECKRGD 211
CY CG+ GH + CN G C+ CG H +C R +
Sbjct: 476 CYNCGDAGHLARDCNRSNNNSGGGGAGCYNCGDTGHLARDCNRSN 610
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 47.4 bits (111), Expect = 3e-06
Identities = 27/101 (26%), Positives = 41/101 (39%), Gaps = 2/101 (1%)
Frame = +2
Query: 129 KIRNERRNKGQQGRPKPYSAPVNKGEQRLNDE--RRPKRRDDPAEIVCYKCGEKGHKSNV 186
K+ ++ R++ + P + D RR RR + +C C GH +
Sbjct: 203 KMSSDSRSRSRSRSRSPMDRKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKRPGHFARE 382
Query: 187 CNGEEKKCFRCGKKWHTIAECKRGDIVCFNCDEEGHLTSQC 227
C C CG H +EC + C+NC E GH+ S C
Sbjct: 383 CPNVAI-CHNCGLPGHIASECTTKSL-CWNCKEPGHMASSC 499
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 42.7 bits (99), Expect = 6e-05
Identities = 22/65 (33%), Positives = 32/65 (48%), Gaps = 6/65 (9%)
Frame = +2
Query: 176 KCGEKGHKSNVCNGEEK---KCFRCGKKWHTIAECKRGD--IVCFNCDEEGHLTSQCK-K 229
K G +G++ + G +CF CG H +CK GD C+ C + GH+ CK
Sbjct: 308 KGGPRGNREYLGRGPPPGSGRCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNS 487
Query: 230 PKKGQ 234
PKK +
Sbjct: 488 PKKNE 502
Score = 36.6 bits (83), Expect = 0.005
Identities = 16/46 (34%), Positives = 21/46 (44%), Gaps = 2/46 (4%)
Frame = +2
Query: 165 RRDDPAEIVCYKCGEKGHKSNVCNGEE--KKCFRCGKKWHTIAECK 208
R P C+ CG GH + C + KC+RCG + H CK
Sbjct: 344 RGPPPGSGRCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCK 481
Score = 30.0 bits (66), Expect = 0.43
Identities = 12/33 (36%), Positives = 16/33 (48%)
Frame = +2
Query: 174 CYKCGEKGHKSNVCNGEEKKCFRCGKKWHTIAE 206
CY+CG++GH C KK +W T E
Sbjct: 437 CYRCGDRGHVERNCKNSPKK-----NEWQTWKE 520
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 42.4 bits (98), Expect = 8e-05
Identities = 19/43 (44%), Positives = 23/43 (53%), Gaps = 3/43 (6%)
Frame = +1
Query: 193 KCFRCGKKWHTIAECKRGD--IVCFNCDEEGHLTSQCK-KPKK 232
+CF CG H +CK GD C+ C E GH+ CK PKK
Sbjct: 388 RCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSPKK 516
Score = 38.1 bits (87), Expect = 0.002
Identities = 19/58 (32%), Positives = 25/58 (42%), Gaps = 2/58 (3%)
Frame = +1
Query: 153 GEQRLNDERRPKRRDDPAEIVCYKCGEKGHKSNVCNGEE--KKCFRCGKKWHTIAECK 208
G R D R P C+ CG GH + C + KC+RCG++ H CK
Sbjct: 328 GGGRDRDREYMGRGPPPGSGRCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCK 501
Score = 30.8 bits (68), Expect = 0.25
Identities = 11/23 (47%), Positives = 13/23 (55%)
Frame = +1
Query: 174 CYKCGEKGHKSNVCNGEEKKCFR 196
CY+CGE+GH C KK R
Sbjct: 457 CYRCGERGHIEKNCKNSPKKLSR 525
>TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (9%)
Length = 450
Score = 36.6 bits (83), Expect = 0.005
Identities = 15/39 (38%), Positives = 18/39 (45%)
Frame = +3
Query: 172 IVCYKCGEKGHKSNVCNGEEKKCFRCGKKWHTIAECKRG 210
+VC C + GH S C G C CG + H EC G
Sbjct: 3 VVCRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYECPSG 119
Score = 30.4 bits (67), Expect = 0.33
Identities = 11/34 (32%), Positives = 16/34 (46%)
Frame = +3
Query: 194 CFRCGKKWHTIAECKRGDIVCFNCDEEGHLTSQC 227
C C + H +C ++C NC GHL +C
Sbjct: 9 CRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYEC 110
Score = 26.2 bits (56), Expect = 6.2
Identities = 8/19 (42%), Positives = 12/19 (63%)
Frame = +3
Query: 212 IVCFNCDEEGHLTSQCKKP 230
+VC NC + GH++ C P
Sbjct: 3 VVCRNCQQLGHMSRDCMGP 59
>TC17929
Length = 791
Score = 31.2 bits (69), Expect = 0.19
Identities = 12/35 (34%), Positives = 17/35 (48%)
Frame = +2
Query: 156 RLNDERRPKRRDDPAEIVCYKCGEKGHKSNVCNGE 190
++ +E + D CY+CGE GHK C E
Sbjct: 8 QIGEETSNRPNDSKFRQTCYRCGESGHKMRNCPKE 112
>AV421754
Length = 416
Score = 29.3 bits (64), Expect = 0.73
Identities = 18/73 (24%), Positives = 34/73 (45%), Gaps = 2/73 (2%)
Frame = +2
Query: 150 VNKGEQRLNDERRPKRRDDPAEIV--CYKCGEKGHKSNVCNGEEKKCFRCGKKWHTIAEC 207
V G ++++D+ + K R D +V K G ++ G K + G+KW +
Sbjct: 140 VTSGLRKVSDDMKTKNRTDRTGVVGSSEKESRAGSRAFSKTGPPKFELQMGRKWVVENQI 319
Query: 208 KRGDIVCFNCDEE 220
+ D+V +CD +
Sbjct: 320 GKKDLVIEDCDSK 358
>AV420911
Length = 418
Score = 28.1 bits (61), Expect = 1.6
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +2
Query: 189 GEEKKCFRCGKKWHTIAECK 208
GE+ KC+ CG+ H EC+
Sbjct: 359 GEDLKCYECGEPGHFARECR 418
Score = 26.6 bits (57), Expect = 4.7
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +2
Query: 171 EIVCYKCGEKGHKSNVC 187
++ CY+CGE GH + C
Sbjct: 365 DLKCYECGEPGHFAREC 415
Score = 26.2 bits (56), Expect = 6.2
Identities = 7/18 (38%), Positives = 11/18 (60%)
Frame = +2
Query: 211 DIVCFNCDEEGHLTSQCK 228
D+ C+ C E GH +C+
Sbjct: 365 DLKCYECGEPGHFARECR 418
>TC17853 similar to UP|Q42412 (Q42412) RNA-binding protein RZ-1, partial
(58%)
Length = 881
Score = 28.1 bits (61), Expect = 1.6
Identities = 12/28 (42%), Positives = 16/28 (56%)
Frame = +2
Query: 174 CYKCGEKGHKSNVCNGEEKKCFRCGKKW 201
C+KCG+ GH + C E + R GK W
Sbjct: 419 CFKCGKPGHFARECPSEGET--RRGKVW 496
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 28.1 bits (61), Expect = 1.6
Identities = 9/20 (45%), Positives = 13/20 (65%)
Frame = +1
Query: 189 GEEKKCFRCGKKWHTIAECK 208
GE+ KC+ CG+ H EC+
Sbjct: 151 GEDLKCYECGEPGHFARECR 210
Score = 26.6 bits (57), Expect = 4.7
Identities = 8/17 (47%), Positives = 12/17 (70%)
Frame = +1
Query: 171 EIVCYKCGEKGHKSNVC 187
++ CY+CGE GH + C
Sbjct: 157 DLKCYECGEPGHFAREC 207
Score = 26.2 bits (56), Expect = 6.2
Identities = 7/18 (38%), Positives = 11/18 (60%)
Frame = +1
Query: 211 DIVCFNCDEEGHLTSQCK 228
D+ C+ C E GH +C+
Sbjct: 157 DLKCYECGEPGHFARECR 210
>TC12591
Length = 523
Score = 27.7 bits (60), Expect = 2.1
Identities = 12/27 (44%), Positives = 16/27 (58%)
Frame = -3
Query: 184 SNVCNGEEKKCFRCGKKWHTIAECKRG 210
S C+ E K F+CG +W+ I KRG
Sbjct: 203 SATCSSELGKNFKCGLRWNLIP*HKRG 123
>TC10525 homologue to UP|AAB94924 (AAB94924) Histone H4, complete
Length = 536
Score = 27.3 bits (59), Expect = 2.8
Identities = 11/32 (34%), Positives = 17/32 (52%)
Frame = +1
Query: 131 RNERRNKGQQGRPKPYSAPVNKGEQRLNDERR 162
R RR + Q+G P+P+ G+ +ERR
Sbjct: 82 RQGRRPEAQEGAPRPHPGHHEAGDSEAGEERR 177
>TC18038 similar to GB|CAC04249.1|9886727|PSA297606 PPF-1 protein {Pisum
sativum;} , partial (20%)
Length = 586
Score = 26.9 bits (58), Expect = 3.6
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +3
Query: 133 ERRNKGQQGRPKPYSAPVNKGEQRLNDERRPKR 165
E NKGQ+ + Y++ V + Q + ERR KR
Sbjct: 192 ESDNKGQEDGEEAYASKVGEEVQSFSRERRSKR 290
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 26.6 bits (57), Expect = 4.7
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = +1
Query: 189 GEEKKCFRCGKKWHTIAECK 208
G + KC+ CG+ H EC+
Sbjct: 364 GSDMKCYECGEPGHFARECR 423
Score = 26.6 bits (57), Expect = 4.7
Identities = 8/18 (44%), Positives = 13/18 (71%)
Frame = +1
Query: 170 AEIVCYKCGEKGHKSNVC 187
+++ CY+CGE GH + C
Sbjct: 367 SDMKCYECGEPGHFAREC 420
Score = 25.8 bits (55), Expect = 8.0
Identities = 7/18 (38%), Positives = 11/18 (60%)
Frame = +1
Query: 211 DIVCFNCDEEGHLTSQCK 228
D+ C+ C E GH +C+
Sbjct: 370 DMKCYECGEPGHFARECR 423
>BP036788
Length = 559
Score = 26.6 bits (57), Expect = 4.7
Identities = 15/48 (31%), Positives = 22/48 (45%)
Frame = +3
Query: 6 ADDWWKSLLPVLEQDGVVVTWAVFRREFLNRYFPEDVRGKKEIEFLEL 53
A+ WW S P + T F R L R+F +D K +E L++
Sbjct: 267 AETWWDSEGPFKPLHAMNPTRLAFVRSVLCRHFKKDPYSAKPLEGLKI 410
>BP079571
Length = 414
Score = 22.7 bits (47), Expect(2) = 5.3
Identities = 7/16 (43%), Positives = 11/16 (68%)
Frame = -1
Query: 174 CYKCGEKGHKSNVCNG 189
C++ GE GH + C+G
Sbjct: 357 CFRFGEVGHLARDCDG 310
Score = 21.9 bits (45), Expect(2) = 5.3
Identities = 7/21 (33%), Positives = 11/21 (52%)
Frame = -3
Query: 202 HTIAECKRGDIVCFNCDEEGH 222
H + G+ CF+C + GH
Sbjct: 298 HVVVVGMLGETHCFHCGKPGH 236
>BP039068
Length = 467
Score = 26.2 bits (56), Expect = 6.2
Identities = 8/15 (53%), Positives = 10/15 (66%)
Frame = +3
Query: 213 VCFNCDEEGHLTSQC 227
VC NC E GH ++ C
Sbjct: 309 VCMNCQETGHASNDC 353
>TC18767
Length = 1004
Score = 26.2 bits (56), Expect = 6.2
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = +2
Query: 190 EEKKCFRCGKKWHTIAECKR 209
+ +CF CG H++ EC R
Sbjct: 155 DASRCFNCGSYNHSLRECSR 214
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.137 0.420
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 4,772,865
Number of Sequences: 28460
Number of extensions: 69398
Number of successful extensions: 427
Number of sequences better than 10.0: 44
Number of HSP's better than 10.0 without gapping: 371
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 409
length of query: 264
length of database: 4,897,600
effective HSP length: 88
effective length of query: 176
effective length of database: 2,393,120
effective search space: 421189120
effective search space used: 421189120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)
Medicago: description of AC137553.2