BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC137510.8 - phase: 0 /pseudo
(254 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV418470 125 9e-30
BP036444 35 0.013
TC19405 similar to UP|Q941C3 (Q941C3) AT4g11980/F16J13_50, parti... 33 0.062
CB827650 30 0.31
TC9074 similar to UP|WR53_ARATH (Q9SUP6) Probable WRKY transcrip... 29 0.90
CN825562 28 1.2
TC17647 weakly similar to UP|AAR06918 (AAR06918) UDP-glycosyltra... 28 2.0
AV422795 28 2.0
TC10539 similar to UP|P82715 (P82715) Thylakoid lumenal 35.8 kDa... 28 2.0
BP028711 27 2.6
BI418736 27 3.4
TC8011 similar to UP|TPIC_FRAAN (Q9M4S8) Triosephosphate isomera... 27 4.4
TC8789 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin att... 26 5.8
TC20147 26 7.6
BF177843 25 9.9
TC8668 similar to UP|Q8W4Q4 (Q8W4Q4) AT4g24800/F6I7_10, partial ... 25 9.9
>AV418470
Length = 271
Score = 125 bits (313), Expect = 9e-30
Identities = 66/92 (71%), Positives = 71/92 (76%)
Frame = +3
Query: 1 MAVTVRTISAALKNLKPFHAPNLIPKKKKNGFFYKMSSSTESPSLTHSITLPSKQSEPVH 60
MAV+VRT+SAALK P HA + I KK G STESP THSITLPSKQSEP H
Sbjct: 6 MAVSVRTVSAALKKFVPTHAFHRISTKKVFG----CKMSTESPPFTHSITLPSKQSEPFH 173
Query: 61 ILAAPGVSSSDFWSAIDSSLFKQWLHNLQTEN 92
I+AAPGVS SDFW+AIDSSLFKQWLHNLQTEN
Sbjct: 174IVAAPGVSHSDFWNAIDSSLFKQWLHNLQTEN 269
>BP036444
Length = 454
Score = 35.0 bits (79), Expect = 0.013
Identities = 24/74 (32%), Positives = 34/74 (45%)
Frame = +2
Query: 14 NLKPFHAPNLIPKKKKNGFFYKMSSSTESPSLTHSITLPSKQSEPVHILAAPGVSSSDFW 73
N K H PN N + +S+S + S TH + P P H++ P +SS FW
Sbjct: 263 NNKQLHVPNT-----PNKYHKYISNSNHNSSSTHEFSCP-----PKHVVNQPIISSIFFW 412
Query: 74 SAIDSSLFKQWLHN 87
I SS Q++ N
Sbjct: 413 KLIISSN*LQYIIN 454
>TC19405 similar to UP|Q941C3 (Q941C3) AT4g11980/F16J13_50, partial (27%)
Length = 514
Score = 32.7 bits (73), Expect = 0.062
Identities = 16/23 (69%), Positives = 17/23 (73%)
Frame = +2
Query: 232 ESVLIKRSSHISKVKRLAFVNMG 254
E LIKR SH KVK+LAF NMG
Sbjct: 68 EEELIKR*SHSCKVKKLAFANMG 136
>CB827650
Length = 551
Score = 30.4 bits (67), Expect = 0.31
Identities = 18/51 (35%), Positives = 30/51 (58%)
Frame = +1
Query: 154 SEGETYAVLTEQARVPVGRIILELPAGMLDDDKGDIVGTAVREVEEETGIK 204
++GE V R G + ++P G +++ + DI A+REV+EETGI+
Sbjct: 373 NKGEVLVVREANGRFR-GTGVWKMPTGSVNEGE-DICDAAIREVKEETGIE 519
>TC9074 similar to UP|WR53_ARATH (Q9SUP6) Probable WRKY transcription
factor 53 (WRKY DNA-binding protein 53), partial (15%)
Length = 1044
Score = 28.9 bits (63), Expect = 0.90
Identities = 17/46 (36%), Positives = 26/46 (55%)
Frame = +3
Query: 209 DMVDLTAFLDSSTGSTVFPSPSAESVLIKRSSHISKVKRLAFVNMG 254
+M+DL FLD+ TVFP + E + SH +V R A +++G
Sbjct: 531 EMIDLDLFLDNMDFDTVFPINTTELCS*YQLSHF-QVNRYAKISVG 665
>CN825562
Length = 658
Score = 28.5 bits (62), Expect = 1.2
Identities = 14/34 (41%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +3
Query: 206 NVEDMVDLTAF-LDSSTGSTVFPSPSAESVLIKR 238
N+ED +L +D+S GST PSP E ++K+
Sbjct: 120 NIEDNKELGQMEVDASAGSTNMPSPQKEEEVVKK 221
>TC17647 weakly similar to UP|AAR06918 (AAR06918) UDP-glycosyltransferase
91D1, partial (13%)
Length = 1059
Score = 27.7 bits (60), Expect = 2.0
Identities = 32/111 (28%), Positives = 47/111 (41%), Gaps = 4/111 (3%)
Frame = -2
Query: 119 FLKFIAEIIDKETGNKVPGIVFARGPAVAMLILL----ESEGETYAVLTEQARVPVGRII 174
F++ A + D+ TG K + + L LL E EGE L + +VPV +
Sbjct: 380 FMRAFAALKDESTG-KSASFDLKKAYSSCDLFLLRTSRELEGEWLDYLADTYKVPVVPVG 204
Query: 175 LELPAGMLDDDKGDIVGTAVREVEEETGIKLNVEDMVDLTAFLDSSTGSTV 225
L P+ + DD EEE D V++ A+LD+ STV
Sbjct: 203 LLPPSMQIRDDD-----------EEEKN-----PDWVEIKAWLDTQEPSTV 99
>AV422795
Length = 419
Score = 27.7 bits (60), Expect = 2.0
Identities = 29/120 (24%), Positives = 49/120 (40%), Gaps = 5/120 (4%)
Frame = +2
Query: 40 TESPSLTHSITLPSKQSEPVHILAAPGVSSSDFWSAIDSSLFKQW-----LHNLQTENGI 94
+ +PSL+H TL S+Q + +H+ +D+ L QW L ++ N +
Sbjct: 80 SSTPSLSHFGTLTSRQKDQIHLY-------------VDALL--QWNKRMNLTAVREVNQV 214
Query: 95 LANDTMTLRQVLIQGVDMFGKRIGFLKFIAEIIDKETGNKVPGIVFARGPAVAMLILLES 154
+ +L D + G ++D TG +PG+V A + LLES
Sbjct: 215 MERHVEDSLAILPPLSDCYRAHCGGSGDKLSLVDVGTGAGLPGVVLAIARPEWDVTLLES 394
>TC10539 similar to UP|P82715 (P82715) Thylakoid lumenal 35.8 kDa protein,
chloroplast precursor, partial (33%)
Length = 645
Score = 27.7 bits (60), Expect = 2.0
Identities = 13/37 (35%), Positives = 20/37 (53%)
Frame = +3
Query: 29 KNGFFYKMSSSTESPSLTHSITLPSKQSEPVHILAAP 65
K F + P++ ++ TLP K SEP +L+AP
Sbjct: 252 KRWLFVLCQCFSPQPTVENNGTLPGKNSEPRSVLSAP 362
>BP028711
Length = 562
Score = 27.3 bits (59), Expect = 2.6
Identities = 16/36 (44%), Positives = 20/36 (55%), Gaps = 1/36 (2%)
Frame = -1
Query: 154 SEGET-YAVLTEQARVPVGRIILELPAGMLDDDKGD 188
SEG + VL E V G + E+ AG DDD+GD
Sbjct: 259 SEGNAGHVVLDEAVDVHDGGAVAEIDAGDEDDDEGD 152
>BI418736
Length = 544
Score = 26.9 bits (58), Expect = 3.4
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = +1
Query: 32 FFYKMSSSTESPSLTHSITLP 52
FF+ SSS SP+ T++ITLP
Sbjct: 55 FFFFKSSSPSSPTNTNTITLP 117
>TC8011 similar to UP|TPIC_FRAAN (Q9M4S8) Triosephosphate isomerase,
chloroplast precursor (TIM) , partial (89%)
Length = 1305
Score = 26.6 bits (57), Expect = 4.4
Identities = 18/69 (26%), Positives = 29/69 (41%), Gaps = 8/69 (11%)
Frame = -3
Query: 14 NLKPFHAPNLIPKKKKNGFFYKMSSS--------TESPSLTHSITLPSKQSEPVHILAAP 65
N P HA P K F+ ++SS +E P +TH S + + AP
Sbjct: 640 NNSPMHAITPRPSVKA*AAFFPINSSFSPMICLRSECPRMTHLTPWSFSCSTLISPVKAP 461
Query: 66 GVSSSDFWS 74
+ + +FW+
Sbjct: 460 PLLTQEFWA 434
>TC8789 weakly similar to UP|AGA1_YEAST (P32323) A-agglutinin attachment
subunit precursor, partial (5%)
Length = 546
Score = 26.2 bits (56), Expect = 5.8
Identities = 16/43 (37%), Positives = 22/43 (50%)
Frame = -1
Query: 37 SSSTESPSLTHSITLPSKQSEPVHILAAPGVSSSDFWSAIDSS 79
SS +E+P +PS EP ++ +P SSS F S SS
Sbjct: 255 SSESETPPFATLFAMPS---EPSSLITSPSKSSSSFSSMTLSS 136
>TC20147
Length = 457
Score = 25.8 bits (55), Expect = 7.6
Identities = 12/35 (34%), Positives = 19/35 (54%)
Frame = -2
Query: 28 KKNGFFYKMSSSTESPSLTHSITLPSKQSEPVHIL 62
KK GFF S+ S+++ + LP ++ HIL
Sbjct: 447 KKTGFFNIFQSAETLSSVSNYVLLPKRKKMYAHIL 343
>BF177843
Length = 474
Score = 25.4 bits (54), Expect = 9.9
Identities = 14/38 (36%), Positives = 17/38 (43%)
Frame = +2
Query: 32 FFYKMSSSTESPSLTHSITLPSKQSEPVHILAAPGVSS 69
FFY + E +T ITL Q+ P IL SS
Sbjct: 143 FFYADGTLAEGNLVTEKITLSPSQTTPPLILGCANTSS 256
>TC8668 similar to UP|Q8W4Q4 (Q8W4Q4) AT4g24800/F6I7_10, partial (39%)
Length = 1216
Score = 25.4 bits (54), Expect = 9.9
Identities = 13/36 (36%), Positives = 16/36 (44%)
Frame = -3
Query: 15 LKPFHAPNLIPKKKKNGFFYKMSSSTESPSLTHSIT 50
L H P+ P F M+S +SPS HS T
Sbjct: 533 LSILHGPSSSPTPAPQKPFTSMASRNQSPSHAHSFT 426
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.317 0.133 0.370
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,544,870
Number of Sequences: 28460
Number of extensions: 44411
Number of successful extensions: 296
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 294
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 294
length of query: 254
length of database: 4,897,600
effective HSP length: 88
effective length of query: 166
effective length of database: 2,393,120
effective search space: 397257920
effective search space used: 397257920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 54 (25.4 bits)
Medicago: description of AC137510.8