BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136954.7 - phase: 0
(396 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC18927 similar to PIR|AI2934|AI2934 chromate transport protein ... 72 1e-13
AU251673 60 8e-10
BI418821 55 2e-08
TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 53 1e-07
TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, part... 49 2e-06
TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, par... 47 4e-06
TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, parti... 40 7e-04
TC9039 35 0.028
TC17929 32 0.24
TC18767 28 2.0
BP042279 28 2.0
BP039068 28 3.5
TC13213 similar to UP|Q84MD2 (Q84MD2) At2g16386, partial (57%) 28 3.5
AV420911 28 3.5
TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 28 3.5
BP065763 27 4.5
TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR... 27 4.5
BP079571 27 5.9
TC8302 27 5.9
TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-li... 27 7.8
>TC18927 similar to PIR|AI2934|AI2934 chromate transport protein chrA
[imported] - Agrobacterium tumefaciens
(strain C58, Dupont) {Agrobacterium tumefaciens;},
partial (6%)
Length = 561
Score = 72.4 bits (176), Expect = 1e-13
Identities = 52/174 (29%), Positives = 73/174 (41%), Gaps = 25/174 (14%)
Frame = -2
Query: 216 RGKGHQNRP--KPYSAPTDKGKQRL--NDERRPSKRDAPAEIVCFKCGEKGHKSNVCTKD 271
R K Q +P +P + T G N RP++ D +EIVC +C +KGH +N
Sbjct: 455 RNKSFQKKPFQRPQNRGTSSGYSHSFGNFVPRPTQSDT-SEIVCHRCSKKGHFAN----- 294
Query: 272 EKKCFRCGQKGHMLADCKRGDVVCYNCNEEGHISTQCTQPK------------------- 312
RC D+VC+NC + GH CT PK
Sbjct: 293 -----RCP------------DLVCWNCQKTGHSGKDCTNPKVEAATNAIAARRPAPAANK 165
Query: 313 --KVRVGGKVFALTGTQTANEDRLIRGTCFFNSTPLIAIIDTGATHCFIALECA 364
+ +V+ ++G ++ D LIR N PL + D+GATH FI L CA
Sbjct: 164 GKRPVASARVYTVSGAESHRADGLIRSVGSVNCKPLTILFDSGATHSFIDLACA 3
>AU251673
Length = 413
Score = 59.7 bits (143), Expect = 8e-10
Identities = 38/138 (27%), Positives = 67/138 (48%), Gaps = 1/138 (0%)
Frame = +2
Query: 70 CSEVQKVRFGTHMLAEEADDWWVSLLPVLEQNGAVVTWAVFRREFLNRYFPEDVRGRKEI 129
CS+ + V + L A DW+ L TWA F EF+NR+ P+ VR
Sbjct: 5 CSDTRAVELASFQLEGVARDWYNVLTRAKPVGSPPWTWADFSAEFMNRFLPQSVRDGFVR 184
Query: 130 EFLELKQGD-MSVVEYAAKFVELAKFYPHYAPETAEFSKCIKFENGLRADIKRAIGYQQI 188
+F L+Q + M+V EY+A F L+++ P+ E + +F GL+ + +++ +
Sbjct: 185 DFERLEQAEGMTVSEYSAHFTHLSRYVPY---PLLEEERVKRFVRGLKEYLFKSVVGSKS 355
Query: 189 RIFSDLVSRCRIYEEDTK 206
S+++S + E+ K
Sbjct: 356 STLSEVLSLALLVEQRQK 409
>BI418821
Length = 614
Score = 55.1 bits (131), Expect = 2e-08
Identities = 30/98 (30%), Positives = 40/98 (40%), Gaps = 15/98 (15%)
Frame = +2
Query: 228 SAPTDKGKQRLNDERRPSKRDAPAEIVCFKCGEKGHKSNVCTKDEKK--------CFRCG 279
SAP G R ERR C+ CG+ GH + C + C+ CG
Sbjct: 314 SAPRGFGGWR-GGERRNGGGGGGGGGGCYNCGDTGHLARDCHRSNNNGGGGGGAACYNCG 490
Query: 280 QKGHMLADCKR-------GDVVCYNCNEEGHISTQCTQ 310
GH+ DC R G CYNC + GH++ C +
Sbjct: 491 DAGHLARDCNRSNNNSGGGGAGCYNCGDTGHLARDCNR 604
Score = 45.8 bits (107), Expect = 1e-05
Identities = 18/52 (34%), Positives = 24/52 (45%), Gaps = 8/52 (15%)
Frame = +2
Query: 275 CFRCGQKGHMLADCKR--------GDVVCYNCNEEGHISTQCTQPKKVRVGG 318
C+ CG GH+ DC R G CYNC + GH++ C + GG
Sbjct: 392 CYNCGDTGHLARDCHRSNNNGGGGGGAACYNCGDAGHLARDCNRSNNNSGGG 547
Score = 40.0 bits (92), Expect = 7e-04
Identities = 14/45 (31%), Positives = 21/45 (46%), Gaps = 7/45 (15%)
Frame = +2
Query: 255 CFKCGEKGHKSNVCTKDEKK-------CFRCGQKGHMLADCKRGD 292
C+ CG+ GH + C + C+ CG GH+ DC R +
Sbjct: 476 CYNCGDAGHLARDCNRSNNNSGGGGAGCYNCGDTGHLARDCNRSN 610
>TC11885 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (26%)
Length = 555
Score = 52.8 bits (125), Expect = 1e-07
Identities = 27/96 (28%), Positives = 43/96 (44%)
Frame = +2
Query: 213 SERRGKGHQNRPKPYSAPTDKGKQRLNDERRPSKRDAPAEIVCFKCGEKGHKSNVCTKDE 272
S R + P +D+ R RR S+R + +C C GH + C +
Sbjct: 218 SRSRSRSRSRSPMDRKIRSDRFSYRDAPYRRDSRRGFSRDNLCKNCKRPGHFARECP-NV 394
Query: 273 KKCFRCGQKGHMLADCKRGDVVCYNCNEEGHISTQC 308
C CG GH+ ++C + C+NC E GH+++ C
Sbjct: 395 AICHNCGLPGHIASECTTKSL-CWNCKEPGHMASSC 499
Score = 35.8 bits (81), Expect = 0.013
Identities = 13/35 (37%), Positives = 20/35 (57%)
Frame = +2
Query: 275 CFRCGQKGHMLADCKRGDVVCYNCNEEGHISTQCT 309
C C + GH +C +C+NC GHI+++CT
Sbjct: 344 CKNCKRPGHFARECPNV-AICHNCGLPGHIASECT 445
>TC9521 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (56%)
Length = 598
Score = 48.5 bits (114), Expect = 2e-06
Identities = 22/44 (50%), Positives = 24/44 (54%), Gaps = 3/44 (6%)
Frame = +1
Query: 274 KCFRCGQKGHMLADCKRGD--VVCYNCNEEGHISTQC-TQPKKV 314
+CF CG GH DCK GD CY C E GHI C PKK+
Sbjct: 388 RCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCKNSPKKL 519
Score = 43.1 bits (100), Expect = 8e-05
Identities = 20/58 (34%), Positives = 28/58 (47%), Gaps = 2/58 (3%)
Frame = +1
Query: 234 GKQRLNDERRPSKRDAPAEIVCFKCGEKGHKSNVCTKDE--KKCFRCGQKGHMLADCK 289
G R D + P CF CG GH + C + KC+RCG++GH+ +CK
Sbjct: 328 GGGRDRDREYMGRGPPPGSGRCFNCGIDGHWARDCKAGDWKNKCYRCGERGHIEKNCK 501
Score = 28.9 bits (63), Expect = 1.6
Identities = 10/23 (43%), Positives = 13/23 (56%)
Frame = +1
Query: 255 CFKCGEKGHKSNVCTKDEKKCFR 277
C++CGE+GH C KK R
Sbjct: 457 CYRCGERGHIEKNCKNSPKKLSR 525
>TC10011 similar to UP|Q9FYA7 (Q9FYA7) Splicing factor RSZ33, partial (62%)
Length = 684
Score = 47.4 bits (111), Expect = 4e-06
Identities = 23/57 (40%), Positives = 30/57 (52%), Gaps = 5/57 (8%)
Frame = +2
Query: 274 KCFRCGQKGHMLADCKRGD--VVCYNCNEEGHISTQC-TQPKK--VRVGGKVFALTG 325
+CF CG GH DCK GD CY C + GH+ C PKK + ++F+ TG
Sbjct: 368 RCFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCKNSPKKNEWQTWKELFSFTG 538
Score = 41.2 bits (95), Expect = 3e-04
Identities = 16/37 (43%), Positives = 22/37 (59%), Gaps = 2/37 (5%)
Frame = +2
Query: 255 CFKCGEKGHKSNVCTKDE--KKCFRCGQKGHMLADCK 289
CF CG GH + C + KC+RCG +GH+ +CK
Sbjct: 371 CFNCGLDGHWARDCKAGDWKNKCYRCGDRGHVERNCK 481
Score = 27.3 bits (59), Expect = 4.5
Identities = 8/20 (40%), Positives = 12/20 (60%)
Frame = +2
Query: 255 CFKCGEKGHKSNVCTKDEKK 274
C++CG++GH C KK
Sbjct: 437 CYRCGDRGHVERNCKNSPKK 496
>TC13053 similar to UP|Q8LF59 (Q8LF59) DNA-binding protein, partial (9%)
Length = 450
Score = 40.0 bits (92), Expect = 7e-04
Identities = 14/34 (41%), Positives = 20/34 (58%)
Frame = +3
Query: 275 CFRCGQKGHMLADCKRGDVVCYNCNEEGHISTQC 308
C C Q GHM DC ++C+NC GH++ +C
Sbjct: 9 CRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYEC 110
Score = 36.6 bits (83), Expect = 0.007
Identities = 14/39 (35%), Positives = 19/39 (47%)
Frame = +3
Query: 253 IVCFKCGEKGHKSNVCTKDEKKCFRCGQKGHMLADCKRG 291
+VC C + GH S C C CG +GH+ +C G
Sbjct: 3 VVCRNCQQLGHMSRDCMGPLMICHNCGGRGHLAYECPSG 119
Score = 27.7 bits (60), Expect = 3.5
Identities = 10/19 (52%), Positives = 12/19 (62%)
Frame = +3
Query: 293 VVCYNCNEEGHISTQCTQP 311
VVC NC + GH+S C P
Sbjct: 3 VVCRNCQQLGHMSRDCMGP 59
>TC9039
Length = 1218
Score = 34.7 bits (78), Expect = 0.028
Identities = 25/85 (29%), Positives = 38/85 (44%), Gaps = 6/85 (7%)
Frame = +2
Query: 192 SDLVSRCRIYEEDTKAHYKVMSERRGKGHQNRPKPYSAPTDKGKQRLNDERRPSKRDAPA 251
++L+S C + EE+ ++ ER+ H S DKGK++ E + DAPA
Sbjct: 671 NELISFC-VQEEE-----RLKQERKESAHF-----VSTSKDKGKRKKTVEPKNEAADAPA 817
Query: 252 ------EIVCFKCGEKGHKSNVCTK 270
+ C+ C GH CTK
Sbjct: 818 PKKQKEDDTCYFCNVSGHMKKKCTK 892
>TC17929
Length = 791
Score = 31.6 bits (70), Expect = 0.24
Identities = 11/37 (29%), Positives = 19/37 (50%)
Frame = +2
Query: 235 KQRLNDERRPSKRDAPAEIVCFKCGEKGHKSNVCTKD 271
+ ++ +E D+ C++CGE GHK C K+
Sbjct: 2 RAQIGEETSNRPNDSKFRQTCYRCGESGHKMRNCPKE 112
Score = 28.5 bits (62), Expect = 2.0
Identities = 8/22 (36%), Positives = 15/22 (67%)
Frame = +2
Query: 269 TKDEKKCFRCGQKGHMLADCKR 290
+K + C+RCG+ GH + +C +
Sbjct: 44 SKFRQTCYRCGESGHKMRNCPK 109
>TC18767
Length = 1004
Score = 28.5 bits (62), Expect = 2.0
Identities = 9/21 (42%), Positives = 12/21 (56%)
Frame = +2
Query: 270 KDEKKCFRCGQKGHMLADCKR 290
+D +CF CG H L +C R
Sbjct: 152 EDASRCFNCGSYNHSLRECSR 214
>BP042279
Length = 252
Score = 28.5 bits (62), Expect = 2.0
Identities = 15/61 (24%), Positives = 25/61 (40%), Gaps = 6/61 (9%)
Frame = +1
Query: 255 CFKCGEKGHKSNVCTKDEKKCFRCGQKGHMLADCK------RGDVVCYNCNEEGHISTQC 308
C C G K +C CG +G +++ + + + C +CN G ST C
Sbjct: 13 CGTCNGTGAKPGT---KSSRCSTCGGQGRVVSSTRTPLGIFQQSMTCSSCNGTGETSTPC 183
Query: 309 T 309
+
Sbjct: 184 S 186
>BP039068
Length = 467
Score = 27.7 bits (60), Expect = 3.5
Identities = 10/19 (52%), Positives = 11/19 (57%)
Frame = +3
Query: 250 PAEIVCFKCGEKGHKSNVC 268
P + VC C E GH SN C
Sbjct: 297 PRQTVCMNCQETGHASNDC 353
Score = 26.6 bits (57), Expect = 7.8
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = +3
Query: 294 VCYNCNEEGHISTQC 308
VC NC E GH S C
Sbjct: 309 VCMNCQETGHASNDC 353
>TC13213 similar to UP|Q84MD2 (Q84MD2) At2g16386, partial (57%)
Length = 578
Score = 27.7 bits (60), Expect = 3.5
Identities = 17/50 (34%), Positives = 20/50 (40%)
Frame = +2
Query: 2 AGRNDAAIAAALEAVAQAVQQQPQAGNGEVRMLETFLRNHPPAFKGRYDP 51
AGR I AVQ++P EVR + L GRYDP
Sbjct: 113 AGRGSKLIRNLAADPVNAVQEEPDLNTEEVRNIHERLLRANTRDYGRYDP 262
>AV420911
Length = 418
Score = 27.7 bits (60), Expect = 3.5
Identities = 7/19 (36%), Positives = 13/19 (67%)
Frame = +2
Query: 271 DEKKCFRCGQKGHMLADCK 289
++ KC+ CG+ GH +C+
Sbjct: 362 EDLKCYECGEPGHFARECR 418
Score = 27.3 bits (59), Expect = 4.5
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +2
Query: 292 DVVCYNCNEEGHISTQC 308
D+ CY C E GH + +C
Sbjct: 365 DLKCYECGEPGHFAREC 415
>TC18003 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (42%)
Length = 587
Score = 27.7 bits (60), Expect = 3.5
Identities = 7/19 (36%), Positives = 13/19 (67%)
Frame = +1
Query: 271 DEKKCFRCGQKGHMLADCK 289
++ KC+ CG+ GH +C+
Sbjct: 154 EDLKCYECGEPGHFARECR 210
Score = 27.3 bits (59), Expect = 4.5
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +1
Query: 292 DVVCYNCNEEGHISTQC 308
D+ CY C E GH + +C
Sbjct: 157 DLKCYECGEPGHFAREC 207
>BP065763
Length = 537
Score = 27.3 bits (59), Expect = 4.5
Identities = 19/62 (30%), Positives = 25/62 (39%), Gaps = 9/62 (14%)
Frame = -1
Query: 260 EKGHKSNVCTKD-----EKKCFRCGQKGHMLADCKRGDV----VCYNCNEEGHISTQCTQ 310
EK H ++ T D + K R QK K G + VCY C + GH C +
Sbjct: 513 EKDHSIHLTTHDGASXSKAKPGRKEQKKDQALKVKEGRIHKEHVCYFCKKAGHFKKYCPK 334
Query: 311 PK 312
K
Sbjct: 333 RK 328
>TC11095 similar to PIR|T05112|T05112 splicing factor 9G8-like SR protein
RSZp22 [validated] - Arabidopsis thaliana
{Arabidopsis thaliana;}, partial (89%)
Length = 912
Score = 27.3 bits (59), Expect = 4.5
Identities = 8/17 (47%), Positives = 11/17 (64%)
Frame = +1
Query: 292 DVVCYNCNEEGHISTQC 308
D+ CY C E GH + +C
Sbjct: 370 DMKCYECGEPGHFAREC 420
Score = 27.3 bits (59), Expect = 4.5
Identities = 7/18 (38%), Positives = 12/18 (65%)
Frame = +1
Query: 272 EKKCFRCGQKGHMLADCK 289
+ KC+ CG+ GH +C+
Sbjct: 370 DMKCYECGEPGHFARECR 423
>BP079571
Length = 414
Score = 26.9 bits (58), Expect = 5.9
Identities = 10/22 (45%), Positives = 13/22 (58%)
Frame = -1
Query: 275 CFRCGQKGHMLADCKRGDVVCY 296
CFR G+ GH+ DC G V +
Sbjct: 357 CFRFGEVGHLARDCDGGVAVTW 292
>TC8302
Length = 494
Score = 26.9 bits (58), Expect = 5.9
Identities = 16/57 (28%), Positives = 24/57 (42%)
Frame = +3
Query: 232 DKGKQRLNDERRPSKRDAPAEIVCFKCGEKGHKSNVCTKDEKKCFRCGQKGHMLADC 288
++ QR N E R P E + G + + KC+ CGQ+ H+ DC
Sbjct: 237 EEDSQR*NKEDR*DS-SKPMEALTMTRGRSKSRKKI----NLKCYHCGQR*HLKKDC 392
>TC20129 homologue to GB|AAF04891.1|6175165|ATAC011437 Mutator-like
transposase {Arabidopsis thaliana;} , partial (12%)
Length = 588
Score = 26.6 bits (57), Expect = 7.8
Identities = 12/30 (40%), Positives = 14/30 (46%)
Frame = +3
Query: 293 VVCYNCNEEGHISTQCTQPKKVRVGGKVFA 322
V C CN+ GH T C P + V V A
Sbjct: 219 VHCSRCNQTGHFRTTCAAPI*INVSFLVCA 308
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.136 0.413
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 7,168,313
Number of Sequences: 28460
Number of extensions: 105012
Number of successful extensions: 520
Number of sequences better than 10.0: 43
Number of HSP's better than 10.0 without gapping: 451
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 498
length of query: 396
length of database: 4,897,600
effective HSP length: 92
effective length of query: 304
effective length of database: 2,279,280
effective search space: 692901120
effective search space used: 692901120
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 56 (26.2 bits)
Medicago: description of AC136954.7