BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136953.11 + phase: 0 /pseudo
(615 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
AV411435 57 9e-09
CN825238 50 8e-07
AV428268 43 1e-04
BE122611 42 2e-04
AV412895 41 6e-04
TC19933 41 6e-04
CB826728 39 0.003
TC15157 weakly similar to GB|AAM19768.1|20453045|AY094388 AT3g56... 33 0.18
AU089037 33 0.18
AV780250 31 0.67
BP056597 29 2.5
BP041113 29 2.5
TC15715 similar to PIR|S33804|S33804 male sterility protein 2 - ... 29 2.5
TC9438 weakly similar to UP|Q8LBI4 (Q8LBI4) Nicotiana lesion-ind... 29 2.5
TC16855 similar to UP|Q8VAC7 (Q8VAC7) Wsv498, partial (15%) 28 3.3
TC9301 similar to UP|Q7Y0Z0 (Q7Y0Z0) TPR1, complete 28 3.3
TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B pre... 28 4.3
TC18821 weakly similar to UP|SRP_DROME (P52172) Box A-binding fa... 27 7.4
CB828677 27 7.4
BI420645 27 9.6
>AV411435
Length = 426
Score = 57.0 bits (136), Expect = 9e-09
Identities = 33/112 (29%), Positives = 48/112 (42%), Gaps = 6/112 (5%)
Frame = +3
Query: 254 GNHDVPV*KFERDGAYSVKSAYTNI------LNHDVAVVHHRVPDNWTCIWSLKFPSKVK 307
G+ D F G YSVKS Y + L++ + H W IW + P KV+
Sbjct: 30 GSEDRLFWPFIPSGEYSVKSGYRAVKATQFNLSNLASSSSHPSSFLWHTIWGAQVPKKVR 209
Query: 308 IFLWRACRNCLPTRIRLQSKGVQCTDKCAVRDDFAEDSTHLFFMCNKSMICW 359
FLWRA N +P + L+ + + D C + + ED H C + W
Sbjct: 210 SFLWRAANNAIPVKRNLKRRNMGRDDFCPICNKGPEDINHALLACEWTRAVW 365
>CN825238
Length = 692
Score = 50.4 bits (119), Expect = 8e-07
Identities = 28/90 (31%), Positives = 44/90 (48%), Gaps = 3/90 (3%)
Frame = +3
Query: 262 KFERDGAYSVKSAYTNILNHDVAVVHHRVPDN--WTCIWSLKFPSKVKIFLWRACRNCLP 319
++ R+GA+SV+SAY NI + W +W P+K+K F +R RN +P
Sbjct: 423 RWSRNGAFSVRSAYYNIQVAKEMQTSSSAASSFPWKKLWRTPVPNKMKHFAYRVARNIIP 602
Query: 320 TRIRLQSKGVQCTDKC-AVRDDFAEDSTHL 348
R LQ +G+ ++C E +HL
Sbjct: 603 CRENLQRRGIAVQNECPTCNHQIPESMSHL 692
>AV428268
Length = 429
Score = 43.1 bits (100), Expect = 1e-04
Identities = 28/85 (32%), Positives = 41/85 (47%), Gaps = 1/85 (1%)
Frame = +3
Query: 269 YSVKSAYTNILNHDVAVVHHRVPDN-WTCIWSLKFPSKVKIFLWRACRNCLPTRIRLQSK 327
YSVKSAY + H A DN + +WS+K PS W+ N + T++ L +
Sbjct: 6 YSVKSAYDFL--HGCANAQW---DNIFKLLWSVKVPSNAIALSWKVLINRVQTKVNLNRR 170
Query: 328 GVQCTDKCAVRDDFAEDSTHLFFMC 352
GV ++ C + E + HL F C
Sbjct: 171 GVVISNVCPLCSLDEESTDHLLFSC 245
>BE122611
Length = 357
Score = 42.4 bits (98), Expect = 2e-04
Identities = 23/60 (38%), Positives = 29/60 (48%)
Frame = -3
Query: 264 ERDGAYSVKSAYTNILNHDVAVVHHRVPDNWTCIWSLKFPSKVKIFLWRACRNCLPTRIR 323
E +G YS KS Y N+L D W+ +W P KV+ FLW RN LP+ R
Sbjct: 310 ESNGCYSAKSTY-NLLIEDGG----NESRFWSLVWKANAPEKVRFFLWLVGRNALPSNAR 146
>AV412895
Length = 405
Score = 40.8 bits (94), Expect = 6e-04
Identities = 23/73 (31%), Positives = 33/73 (44%), Gaps = 3/73 (4%)
Frame = +1
Query: 297 IWSLKFPSKVKIFLWRACRNCLPTRIRLQSKGV--QCTDKCAVRDDFAEDS-THLFFMCN 353
IW + P +K F+WR + TR L + V D AE+S +HL F C
Sbjct: 82 IWMVPAPLNIKAFVWRVLLGRIQTRDNLLKRQVIHNALDAICPLCGLAEESGSHLLFSCA 261
Query: 354 KSMICWQRSGLWI 366
+SM+ W W+
Sbjct: 262 ESMLIWYECFAWL 300
>TC19933
Length = 472
Score = 40.8 bits (94), Expect = 6e-04
Identities = 17/46 (36%), Positives = 25/46 (53%)
Frame = -3
Query: 294 WTCIWSLKFPSKVKIFLWRACRNCLPTRIRLQSKGVQCTDKCAVRD 339
W+ I K +KVK FLW+ C N +P + L+ +G KC + D
Sbjct: 143 WSSI*KAKVSNKVKHFLWKLCHNAIPLKGNLRRRGCATDGKCPICD 6
>CB826728
Length = 509
Score = 38.5 bits (88), Expect = 0.003
Identities = 25/93 (26%), Positives = 36/93 (37%), Gaps = 6/93 (6%)
Frame = +1
Query: 266 DGAYSVKSAYTNILNHDVAVVHHRVPDN------WTCIWSLKFPSKVKIFLWRACRNCLP 319
DG YS KS Y I + +++ P W +WS + K +WRAC LP
Sbjct: 229 DGWYSSKSGYEFIRLENKSLLPSTSPAAAVPSLIWKTVWSASSLPRCKEVMWRACAGYLP 408
Query: 320 TRIRLQSKGVQCTDKCAVRDDFAEDSTHLFFMC 352
R L+ +G+ E H+ C
Sbjct: 409 VRSALKRRGLDVDPSSPWSGLEDEAEVHVLLTC 507
>TC15157 weakly similar to GB|AAM19768.1|20453045|AY094388
AT3g56200/F18O21_160 {Arabidopsis thaliana;}, partial
(31%)
Length = 936
Score = 32.7 bits (73), Expect = 0.18
Identities = 17/70 (24%), Positives = 29/70 (41%), Gaps = 10/70 (14%)
Frame = -2
Query: 300 LKFPSKVKIFLWRACRNCLPTRIRLQSKGVQCTDKC----------AVRDDFAEDSTHLF 349
+ P+K ++++W++C N L +I K + C A +ST+
Sbjct: 632 INLPTKGRVYVWKSCSN*LRAKINSGKKLLYVASGCQN*FISEERKEKLSKHANESTNFM 453
Query: 350 FMCNKSMICW 359
C K ICW
Sbjct: 452 TCC*KHCICW 423
>AU089037
Length = 245
Score = 32.7 bits (73), Expect = 0.18
Identities = 19/59 (32%), Positives = 30/59 (50%), Gaps = 3/59 (5%)
Frame = -1
Query: 297 IWSLKFPSKVKIFLWRACRNCLPTRIRLQSKGV-QCTDK--CAVRDDFAEDSTHLFFMC 352
+W + PS F WR + + TR L+ + V Q +++ C + E S+HLFF C
Sbjct: 191 LWKVPAPSNAVSFAWRLILDRVQTRGNLRRRQVIQQSEEALCPMCSQCEESSSHLFFSC 15
>AV780250
Length = 494
Score = 30.8 bits (68), Expect = 0.67
Identities = 20/76 (26%), Positives = 34/76 (44%), Gaps = 6/76 (7%)
Frame = +3
Query: 266 DGAYSVKSAYTNILNHDVAVVHHRVPDNWTC------IWSLKFPSKVKIFLWRACRNCLP 319
+G Y+ KS Y + N V H +P + T +W + + + RA LP
Sbjct: 201 NGEYTSKSGYAVVRNKVV----HALPSSSTALQVSPVVWKAPTLPRCRELIGRAFLKILP 368
Query: 320 TRIRLQSKGVQCTDKC 335
TR L+++G+ + C
Sbjct: 369 TRSALRARGMDVDELC 416
>BP056597
Length = 414
Score = 28.9 bits (63), Expect = 2.5
Identities = 22/76 (28%), Positives = 31/76 (39%)
Frame = +3
Query: 254 GNHDVPV*KFERDGAYSVKSAYTNILNHDVAVVHHRVPDNWTCIWSLKFPSKVKIFLWRA 313
G D V E DG YSVKSAY + D + ++ +W PS WR
Sbjct: 183 GKKDTWVWILEGDGQYSVKSAYDLLSGLDTTI----GISVFSKLWKACAPSNAVALGWRV 350
Query: 314 CRNCLPTRIRLQSKGV 329
+ + T+ L + V
Sbjct: 351 FLDRIQTKDNLSRRHV 398
>BP041113
Length = 503
Score = 28.9 bits (63), Expect = 2.5
Identities = 27/87 (31%), Positives = 33/87 (37%), Gaps = 3/87 (3%)
Frame = +1
Query: 267 GAYSVKSAYTNILNHDVAVVHHRVPDNWTCIWSLKFPSKVKIFLWRACRNCLPTRIRLQS 326
G Y+V SAY+ + +A + IW PS VK F WR LPT L
Sbjct: 253 GTYTVNSAYSFLQAPTLA----DTDPIFATIWRTVAPS-VKAFAWRCLLGRLPTYDNLIK 417
Query: 327 KGV---QCTDKCAVRDDFAEDSTHLFF 350
+ V C E THL F
Sbjct: 418 RQVVVDPAMTVCKFCQGEVESVTHLLF 498
>TC15715 similar to PIR|S33804|S33804 male sterility protein 2 - Arabidopsis
thaliana {Arabidopsis thaliana;}, partial (13%)
Length = 666
Score = 28.9 bits (63), Expect = 2.5
Identities = 28/98 (28%), Positives = 42/98 (42%), Gaps = 6/98 (6%)
Frame = -2
Query: 342 AEDSTHLFFMCNKSMICWQRSGLWILLMAIFYIDLSFPTNVFAILQHLDQQQKQV----F 397
A+DS+H + M C R G+W L++ F + F +N + D +
Sbjct: 494 AQDSSHRLPIPLPFMTCLLRPGMWTLVI*SFQ-SMPFTSNPNSFFSSSDMLSINLCVLLL 318
Query: 398 SVTP--GVYGSIETIRYGTMLLKQLKLFVLV*DVFLLD 433
S P VYGS RY L++F *D+F L+
Sbjct: 317 SNLPP*NVYGS*MLARYFACSTDFLQIFSSF*DIFPLE 204
>TC9438 weakly similar to UP|Q8LBI4 (Q8LBI4) Nicotiana lesion-inducing
like, partial (95%)
Length = 788
Score = 28.9 bits (63), Expect = 2.5
Identities = 10/28 (35%), Positives = 14/28 (49%)
Frame = +1
Query: 340 DFAEDSTHLFFMCNKSMICWQRSGLWIL 367
+F +TH FF CN W S W++
Sbjct: 1 EFGTSNTHFFFFCNSITEQWASSPSWVV 84
>TC16855 similar to UP|Q8VAC7 (Q8VAC7) Wsv498, partial (15%)
Length = 528
Score = 28.5 bits (62), Expect = 3.3
Identities = 16/34 (47%), Positives = 22/34 (64%)
Frame = -2
Query: 25 DRIWKKNQLLK*SLSLASWKGGSH*VCFAIYPFL 58
D+IWK+N+ LK S SL ++ SH* C + FL
Sbjct: 476 DQIWKRNEALKNS-SLMAFGQSSH*TCQLHFFFL 378
>TC9301 similar to UP|Q7Y0Z0 (Q7Y0Z0) TPR1, complete
Length = 1288
Score = 28.5 bits (62), Expect = 3.3
Identities = 27/102 (26%), Positives = 45/102 (43%)
Frame = -3
Query: 352 CNKSMICWQRSGLWILLMAIFYIDLSFPTNVFAILQHLDQQQKQVFSVTPGVYGSIETIR 411
C ++C + S + L+ IF N IL H V + ++++R
Sbjct: 341 CYHLVVCSKFSSSFNSLVTIF--------NAILILPHFQVNSSTVGEKRYILIIQLQSLR 186
Query: 412 YGTMLLKQLKLFVLV*DVFLLDGGMLRLFVILSLSIPLPRMT 453
ML + +KL +LV + RL ++LSL++PL R T
Sbjct: 185 --VMLNRSIKLLLLVRSI-------TRLLLLLSLNLPLLRFT 87
>TC9337 similar to UP|PME_PRUPE (Q43062) Pectinesterase PPE8B precursor
(Pectin methylesterase) (PE) , partial (54%)
Length = 1145
Score = 28.1 bits (61), Expect = 4.3
Identities = 14/71 (19%), Positives = 30/71 (41%)
Frame = +1
Query: 289 RVPDNWTCIWSLKFPSKVKIFLWRACRNCLPTRIRLQSKGVQCTDKCAVRDDFAEDSTHL 348
R +W+ IW L+F + + + + +P++ L+ +G ++
Sbjct: 553 RAQRSWSIIWFLRFSTAISLQILNFYLQLVPSKRTLEDRGRSIQEQ-------------- 690
Query: 349 FFMCNKSMICW 359
F CN+S + W
Sbjct: 691 -FSCNRS*VMW 720
>TC18821 weakly similar to UP|SRP_DROME (P52172) Box A-binding factor (ABF)
(Serpent protein) (GATA-binding factor-B) (Transcription
factor GATA-B) (dGATA-B), partial (3%)
Length = 767
Score = 27.3 bits (59), Expect = 7.4
Identities = 15/43 (34%), Positives = 24/43 (54%)
Frame = +2
Query: 296 CIWSLKFPSKVKIFLWRACRNCLPTRIRLQSKGVQCTDKCAVR 338
C+ L+FP++++ WR C C TRI S+ V + C +R
Sbjct: 356 CLHRLRFPTRLR--RWRRCCRC*RTRIWF-SRVVPVSGGCCLR 475
>CB828677
Length = 520
Score = 27.3 bits (59), Expect = 7.4
Identities = 12/35 (34%), Positives = 20/35 (56%), Gaps = 1/35 (2%)
Frame = +3
Query: 332 TDKC-AVRDDFAEDSTHLFFMCNKSMICWQRSGLW 365
+D C A + D+++ CN++ ICW + GLW
Sbjct: 216 SDACTAYGEGLKYDNSNYVLYCNRA-ICWSKLGLW 317
>BI420645
Length = 551
Score = 26.9 bits (58), Expect = 9.6
Identities = 15/66 (22%), Positives = 39/66 (58%)
Frame = +1
Query: 478 VLALVCVFEMMKVVMC*RRLNG*LRSLMWIWVRL*VYYQLCTGFVICSWVLWTLSLISKL 537
+L ++C+ +++ V +C + +L+ +++ + YQ C G +IC ++ L + S+L
Sbjct: 1 ILLVLCLMDLVGVCLCWKS*TFLSINLVVLFLVIWGSYQACKGMLICLIII--LVV*SQL 174
Query: 538 S*IVFM 543
+ ++F+
Sbjct: 175 ALVIFL 192
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.364 0.161 0.620
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 12,541,563
Number of Sequences: 28460
Number of extensions: 200903
Number of successful extensions: 3192
Number of sequences better than 10.0: 41
Number of HSP's better than 10.0 without gapping: 1735
Number of HSP's successfully gapped in prelim test: 132
Number of HSP's that attempted gapping in prelim test: 1407
Number of HSP's gapped (non-prelim): 1957
length of query: 615
length of database: 4,897,600
effective HSP length: 96
effective length of query: 519
effective length of database: 2,165,440
effective search space: 1123863360
effective search space used: 1123863360
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 14 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 36 (21.5 bits)
S2: 58 (26.9 bits)
Medicago: description of AC136953.11