BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136841.6 + phase: 0
(372 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC10684 206 4e-54
TC14582 171 2e-43
AV410706 140 3e-34
BP066014 114 2e-26
AV426139 70 4e-13
TC15516 43 1e-04
BI419814 40 8e-04
TC18080 similar to GB|AAL90935.1|19699138|AY090274 AT3g18790/MVE... 28 2.5
BI418191 28 3.2
CB829288 27 4.2
TC12271 similar to UP|Q9FVD4 (Q9FVD4) Ser/Thr specific protein p... 27 7.1
AU089648 27 7.1
TC19924 26 9.3
TC10593 weakly similar to UP|Q8T8T9 (Q8T8T9) AT30094p (CG31717-P... 26 9.3
TC12506 26 9.3
TC19969 weakly similar to UP|Q8T8T9 (Q8T8T9) AT30094p (CG31717-P... 26 9.3
AV417978 26 9.3
TC13338 similar to UP|Q9ZTZ4 (Q9ZTZ4) IFA-binding protein, parti... 26 9.3
>TC10684
Length = 564
Score = 206 bits (525), Expect = 4e-54
Identities = 107/143 (74%), Positives = 120/143 (83%), Gaps = 1/143 (0%)
Frame = +3
Query: 231 RNLNLANHDVHPGHRTNLSFKDMCPYGFEDDIFGHLSNM-KPIGQGLIRQEEEIEDLKCY 289
RNL+L NH GH+T+LS+ DMC G +D IFG LSNM +G+GLIRQEEE+EDLKCY
Sbjct: 3 RNLSLDNH----GHKTSLSYNDMCN-GLKDTIFGRLSNMPSSVGKGLIRQEEELEDLKCY 167
Query: 290 VMRFHPGSLQRCAKLRSKEAVNLVRSYSSALFNSEGFDSVDSDDVILTSFSSLKRLVLEA 349
VM FHPGSLQRCA+LRSKEAVNL+ SYS ALFN EG SV+ DD ILTSFSSLKRLVLEA
Sbjct: 168 VMSFHPGSLQRCARLRSKEAVNLIESYSFALFNHEGSGSVEKDDEILTSFSSLKRLVLEA 347
Query: 350 VAFGSFLWETEDYIDNVYKLKDH 372
VAFGSFLWETEDYID +YKLKD+
Sbjct: 348 VAFGSFLWETEDYIDMLYKLKDN 416
>TC14582
Length = 1793
Score = 171 bits (432), Expect = 2e-43
Identities = 115/386 (29%), Positives = 201/386 (51%), Gaps = 21/386 (5%)
Frame = +3
Query: 3 NCLTIKPHIGFFTRPISFSSSLSF-RSRVPL-------IKASAGASSHCESSSLNTPLLP 54
N L I+ + F ++ +F+S+ R R+ + ++A + + SSS+ PL
Sbjct: 219 NLLPIRSNFPFTSKLQTFASNAKQGRGRLSVGARRGFVVRAGSSSPEASGSSSVIAPLKL 398
Query: 55 RTQVGKFLSGVLQNHRNLFHVAVQEELKLLADDRDAANSRMLLASESDEALLHRRIAEMK 114
+ +G+FLS +L +H +L AV ++L+ D + + ++ + +L+RRIAE+K
Sbjct: 399 ESPIGQFLSQILVSHPHLVPAAVDQQLEQFQTDHNELKEKP--SASGTDLVLYRRIAEIK 572
Query: 115 ENQCEVAVEDIMSLLIFHKFSEIRAPLVPKLSRCLYNGRLEILPSKDWELESIHTLEVLD 174
+ A+E+I+ L+ KF + L+P ++ +G+++ PS+ ++E +H+ E +
Sbjct: 573 AKERRKALEEILYTLVVQKFMDANVSLIPSITPNP-SGQVDSWPSEVGKIEGLHSPEAYE 749
Query: 175 MIREHVTTVTGLKAKPSVTESWATTKVRQFLLGRIYVASILYGYFLKSVSLRYHLERNLN 234
MI H+ + G + +S A ++ + G++Y AS++YGYFLK V R+ LE+ +
Sbjct: 750 MIENHLGLLLGSRHG----DSTAVAQISKIRAGQVYAASVMYGYFLKRVVQRFQLEKAIK 917
Query: 235 LANH--DVHPGHRTNLSFKDMCPYGFEDDIFGHLSNM----KPIGQGLIRQEEEIEDLKC 288
+ + + + HRT + + F H I G Q + L
Sbjct: 918 ILPNAAEENNSHRTIVDDTRVSIEEFPSQAMPHPEVSAWPGSGISSGAFGQGTTVSRLHT 1097
Query: 289 YVMRFHPGSLQRCAKLRSKEAVNLVRSYSSALFNS-------EGFDSVDSDDVILTSFSS 341
Y+M F +LQR A +RSKE+V+++ ++ ALF EG + D++I SF
Sbjct: 1098YMMSFDGETLQRYATIRSKESVSIIEKHTEALFGRPDAVITPEGKINSSKDEIIKISFGG 1277
Query: 342 LKRLVLEAVAFGSFLWETEDYIDNVY 367
LKRLVLEAV FGSFLW+ E Y+D+ Y
Sbjct: 1278LKRLVLEAVTFGSFLWDVESYVDSRY 1355
>AV410706
Length = 427
Score = 140 bits (353), Expect = 3e-34
Identities = 79/116 (68%), Positives = 90/116 (77%), Gaps = 5/116 (4%)
Frame = +3
Query: 14 FTRPISFSSSLSFRS-----RVPLIKASAGASSHCESSSLNTPLLPRTQVGKFLSGVLQN 68
FTRPIS SS S R IKASAG S LN+PL PR+ VGKFLSGVLQN
Sbjct: 96 FTRPISVVSSSSLHLTCRPIRRAKIKASAGVCS------LNSPLEPRSLVGKFLSGVLQN 257
Query: 69 HRNLFHVAVQEELKLLADDRDAANSRMLLASESDEALLHRRIAEMKENQCEVAVED 124
HR LFHVAV EELKLLADDRDAA++R++LAS+SDE+LLHRRIA++KENQC+VAVED
Sbjct: 258 HRQLFHVAVGEELKLLADDRDAAHARLILASDSDESLLHRRIAQLKENQCQVAVED 425
>BP066014
Length = 535
Score = 114 bits (286), Expect = 2e-26
Identities = 67/104 (64%), Positives = 77/104 (73%), Gaps = 5/104 (4%)
Frame = +1
Query: 14 FTRPISFSSSLSFRS-----RVPLIKASAGASSHCESSSLNTPLLPRTQVGKFLSGVLQN 68
FTRPIS SS S R IKASAG S LN+PL PR+ VGKFLSGVLQN
Sbjct: 157 FTRPISVVSSSSLHLTCRPIRRAKIKASAGVCS------LNSPLEPRSLVGKFLSGVLQN 318
Query: 69 HRNLFHVAVQEELKLLADDRDAANSRMLLASESDEALLHRRIAE 112
HR LFHVAV EELKLLADDRDAA++R++LAS+SDE+LLHR +A+
Sbjct: 319 HRQLFHVAVGEELKLLADDRDAAHARLILASDSDESLLHRFVAK 450
>AV426139
Length = 387
Score = 70.5 bits (171), Expect = 4e-13
Identities = 35/120 (29%), Positives = 70/120 (58%)
Frame = +1
Query: 116 NQCEVAVEDIMSLLIFHKFSEIRAPLVPKLSRCLYNGRLEILPSKDWELESIHTLEVLDM 175
N+ A+E+I+ L+ KF L+P ++ GR++ P+ D +L+ +H+ E +M
Sbjct: 4 NERRKALEEILYALVVQKFMAANISLIPSITPDP-TGRVDSWPNDDEKLKQLHSYEAYEM 180
Query: 176 IREHVTTVTGLKAKPSVTESWATTKVRQFLLGRIYVASILYGYFLKSVSLRYHLERNLNL 235
I+ H++ + G +A + + ++ + +G++Y AS++YGYFLK V R+ LE+ + +
Sbjct: 181 IQNHLSLILGNRAG----DLTSIAQISKLRVGQVYAASVMYGYFLKRVDQRFQLEKTMKV 348
>TC15516
Length = 512
Score = 42.7 bits (99), Expect = 1e-04
Identities = 26/70 (37%), Positives = 41/70 (58%)
Frame = +3
Query: 21 SSSLSFRSRVPLIKASAGASSHCESSSLNTPLLPRTQVGKFLSGVLQNHRNLFHVAVQEE 80
S S+ R R +++A+A S ES++ PL + +G+FLS +L NH +L AV ++
Sbjct: 246 SFSVGTRRRALVVRAAASDSD--ESANKIAPLQLESPIGQFLSQILINHPHLVPAAVDQQ 419
Query: 81 LKLLADDRDA 90
L L DRD+
Sbjct: 420 LLQLQTDRDS 449
>BI419814
Length = 527
Score = 39.7 bits (91), Expect = 8e-04
Identities = 24/83 (28%), Positives = 44/83 (52%)
Frame = +2
Query: 17 PISFSSSLSFRSRVPLIKASAGASSHCESSSLNTPLLPRTQVGKFLSGVLQNHRNLFHVA 76
P ++ + R+R +++ASA +S + S+ PL + VG+ L ++QNH +L A
Sbjct: 275 PKHYNELYNSRTRGLVMRASADSSDNLVPSA---PLQFESPVGQLLGQIMQNHSHLLSTA 445
Query: 77 VQEELKLLADDRDAANSRMLLAS 99
+ ++L+ L RDA +S
Sbjct: 446 IDQQLENLQTARDAPKEESFTSS 514
>TC18080 similar to GB|AAL90935.1|19699138|AY090274 AT3g18790/MVE11_17
{Arabidopsis thaliana;}, partial (24%)
Length = 585
Score = 28.1 bits (61), Expect = 2.5
Identities = 21/38 (55%), Positives = 25/38 (65%)
Frame = -3
Query: 15 TRPISFSSSLSFRSRVPLIKASAGASSHCESSSLNTPL 52
T +SFSSSLSF S +PLI +S+ SS SSSL L
Sbjct: 175 TNSLSFSSSLSFIS-LPLILSSSTTSS---SSSLRISL 74
>BI418191
Length = 652
Score = 27.7 bits (60), Expect = 3.2
Identities = 16/55 (29%), Positives = 24/55 (43%)
Frame = +1
Query: 301 CAKLRSKEAVNLVRSYSSALFNSEGFDSVDSDDVILTSFSSLKRLVLEAVAFGSF 355
C LR++E+V++ R + S F S + TSF L + GSF
Sbjct: 346 CLTLRTRESVSI*RPFCIVYMASSWFTDPSSGRPLTTSFIGLYMRPRDTAVLGSF 510
>CB829288
Length = 451
Score = 27.3 bits (59), Expect = 4.2
Identities = 18/57 (31%), Positives = 29/57 (50%), Gaps = 5/57 (8%)
Frame = +2
Query: 115 ENQCEVAVEDIMSLLIFHKFSEIRA-----PLVPKLSRCLYNGRLEILPSKDWELES 166
ENQC AV + + H +S +R P +SRC+ G LEI ++ +++S
Sbjct: 281 ENQCASAVVKHIRAPVPHVWSLVRRFDQPQKYKPFVSRCIVRGNLEIGSLREVDVKS 451
>TC12271 similar to UP|Q9FVD4 (Q9FVD4) Ser/Thr specific protein phosphatase
2A B regulatory subunit beta isoform, partial (9%)
Length = 406
Score = 26.6 bits (57), Expect = 7.1
Identities = 13/24 (54%), Positives = 15/24 (62%)
Frame = -1
Query: 46 SSLNTPLLPRTQVGKFLSGVLQNH 69
SSLN P R+ V FLSG Q+H
Sbjct: 265 SSLNQPTNQRSLVSDFLSGFSQDH 194
>AU089648
Length = 440
Score = 26.6 bits (57), Expect = 7.1
Identities = 15/37 (40%), Positives = 21/37 (56%), Gaps = 1/37 (2%)
Frame = -3
Query: 110 IAEMKENQCE-VAVEDIMSLLIFHKFSEIRAPLVPKL 145
I + N+C V I S +I+ KFS+IR LVP +
Sbjct: 177 ITXLVSNRCR*V*SSPIKSCVIYSKFSQIRQNLVPSI 67
>TC19924
Length = 525
Score = 26.2 bits (56), Expect = 9.3
Identities = 14/32 (43%), Positives = 18/32 (55%)
Frame = -1
Query: 135 SEIRAPLVPKLSRCLYNGRLEILPSKDWELES 166
+ + PLVP L RC NGR LP + W+ S
Sbjct: 330 ASFKLPLVPDLLRCSRNGRACNLPLQ-WDQAS 238
>TC10593 weakly similar to UP|Q8T8T9 (Q8T8T9) AT30094p (CG31717-PA), partial
(18%)
Length = 674
Score = 26.2 bits (56), Expect = 9.3
Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 1/24 (4%)
Frame = +1
Query: 195 SWA-TTKVRQFLLGRIYVASILYG 217
SWA TT V + +LGR Y+ +L+G
Sbjct: 37 SWALTTVVSRVVLGRHYILDVLFG 108
>TC12506
Length = 393
Score = 26.2 bits (56), Expect = 9.3
Identities = 20/46 (43%), Positives = 25/46 (53%), Gaps = 1/46 (2%)
Frame = -2
Query: 4 CLTIKP-HIGFFTRPISFSSSLSFRSRVPLIKASAGASSHCESSSL 48
CLT +P +I FF IS S+ S PLI +S +SS SSL
Sbjct: 170 CLTYQPGNIWFFCTSISVEPSV*CSS*TPLILSSDNSSSLEPFSSL 33
>TC19969 weakly similar to UP|Q8T8T9 (Q8T8T9) AT30094p (CG31717-PA), partial
(13%)
Length = 480
Score = 26.2 bits (56), Expect = 9.3
Identities = 12/24 (50%), Positives = 17/24 (70%), Gaps = 1/24 (4%)
Frame = +1
Query: 195 SWA-TTKVRQFLLGRIYVASILYG 217
SWA TT V + +LGR Y+ +L+G
Sbjct: 76 SWALTTVVSRVVLGRHYILDVLFG 147
>AV417978
Length = 374
Score = 26.2 bits (56), Expect = 9.3
Identities = 20/59 (33%), Positives = 28/59 (46%), Gaps = 2/59 (3%)
Frame = +3
Query: 6 TIKPHIGFFTR--PISFSSSLSFRSRVPLIKASAGASSHCESSSLNTPLLPRTQVGKFL 62
T+ P FFT P SSSL+ + PL + +S S+L LL + GKF+
Sbjct: 108 TLSPFSTFFTPKPPCVSSSSLTHQHPYPLSTTTELSSKPLSLSTLPRKLLCKPPQGKFV 284
>TC13338 similar to UP|Q9ZTZ4 (Q9ZTZ4) IFA-binding protein, partial (12%)
Length = 530
Score = 26.2 bits (56), Expect = 9.3
Identities = 23/86 (26%), Positives = 38/86 (43%)
Frame = +1
Query: 34 KASAGASSHCESSSLNTPLLPRTQVGKFLSGVLQNHRNLFHVAVQEELKLLADDRDAANS 93
K G+ SH ESS+ NTP+ +L L N +++ E+LKLL +
Sbjct: 196 KEENGSYSHQESSNQNTPV----DAALYLEESLANFEE-ERLSILEQLKLLEE------- 339
Query: 94 RMLLASESDEALLHRRIAEMKENQCE 119
++++ S +E E+ CE
Sbjct: 340 KLVILSYEEEHSFEFDDTNSNEHLCE 417
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.321 0.136 0.394
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,435,136
Number of Sequences: 28460
Number of extensions: 88331
Number of successful extensions: 449
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 443
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 443
length of query: 372
length of database: 4,897,600
effective HSP length: 92
effective length of query: 280
effective length of database: 2,279,280
effective search space: 638198400
effective search space used: 638198400
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC136841.6