Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC136838.2 - phase: 2 
         (90 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

TC7796 homologue to gb|AF036495.1|AF036495 Hamamelis virginiana ...   172  1e-44
TC19186 homologue to emb|Z18289.1|PPC16SRNA Palmaria palmata Chl...    31  0.043
AV776603                                                               28  0.28
TC14158 homologue to gb|M75722.1|ALSCP23SA Alnus incana chloropl...    27  0.62
TC8864                                                                 25  1.8
AU251869                                                               25  1.8
TC8360 weakly similar to UP|O22940 (O22940) At2g41800 protein (A...    25  2.4
TC19448 homologue to UP|O76915 (O76915) NG3 protein, partial (11%)     25  2.4
BP066148                                                               25  2.4
TC9953 similar to GB|CAD35362.1|21535744|ATH490171 FK506 binding...    25  2.4
TC12903 weakly similar to UP|ALA9_ARATH (Q9SX33) Potential phosp...    25  3.1
TC8032 similar to UP|Q9M0B6 (Q9M0B6) Nucleotide sugar epimerase-...    25  3.1
TC9308 weakly similar to UP|Q9SQ55 (Q9SQ55) Nuclear RNA binding ...    25  3.1
TC15711 weakly similar to UP|MID2_YEAST (P36027) Mating process ...    25  3.1
TC17082                                                                25  3.1
BP058189                                                               24  4.0
TC11170 weakly similar to UP|Q9FKT4 (Q9FKT4) RNA-binding protein...    24  4.0
TC19667 similar to UP|O24078 (O24078) Protein phosphatase 2C, pa...    24  4.0
AV767360                                                               24  4.0
BP057603                                                               24  5.3

>TC7796 homologue to gb|AF036495.1|AF036495 Hamamelis virginiana large
            subunit 26S ribosomal RNA gene, partial sequence,
            complete
          Length = 6006

 Score =  172 bits (435), Expect = 1e-44
 Identities = 82/90 (91%), Positives = 83/90 (92%)
 Frame = -1

Query: 1    LGRNLPPDWGCIPKQPDSPTTPRGATGSEHNGALTLSGAPFQGTWARSAAEDASPYYNSN 60
            LGRNLPPD GCIPKQPDSPT PRGATGS HNGALTLSGAPFQGTWARSA EDASP YNS+
Sbjct: 2901 LGRNLPPD*GCIPKQPDSPTAPRGATGSGHNGALTLSGAPFQGTWARSATEDASPDYNSD 2722

Query: 61   AEGDRFSWWAYPGSLAVTKGILVSFFSSAY 90
            A GDRFSWWA PGSLAVTKGILVSFFSSAY
Sbjct: 2721 ALGDRFSWWALPGSLAVTKGILVSFFSSAY 2632


>TC19186 homologue to emb|Z18289.1|PPC16SRNA Palmaria palmata Chloroplast
           for 16SrRNA gene, partial (10%)
          Length = 563

 Score = 30.8 bits (68), Expect = 0.043
 Identities = 14/21 (66%), Positives = 15/21 (70%)
 Frame = -1

Query: 70  AYPGSLAVTKGILVSFFSSAY 90
           A P SLA TKGI  +FFSS Y
Sbjct: 230 AVPASLAATKGIAFAFFSSGY 168


>AV776603 
          Length = 359

 Score = 28.1 bits (61), Expect = 0.28
 Identities = 21/72 (29%), Positives = 32/72 (44%), Gaps = 5/72 (6%)
 Frame = +2

Query: 7   PDWGCIPKQPDSPTTPRG---ATGSEHNGALTLSGAPFQGTWARSAAEDASPYYNS--NA 61
           P W    K  DSP T  G      S  +G   LS A + G+ + ++  D    +N+  N 
Sbjct: 104 PGWSSAKKSNDSPATGWGQGSGVKSGSDGGANLSSA-WGGSKSGTSNTDVGAGWNNKPNK 280

Query: 62  EGDRFSWWAYPG 73
           +G++ S W   G
Sbjct: 281 DGNKASGWGQAG 316


>TC14158 homologue to gb|M75722.1|ALSCP23SA Alnus incana chloroplast 23S
           ribosomal RNA (23S rRNA) gene, partial (87%)
          Length = 2528

 Score = 26.9 bits (58), Expect = 0.62
 Identities = 21/64 (32%), Positives = 27/64 (41%)
 Frame = -3

Query: 27  GSEHNGALTLSGAPFQGTWARSAAEDASPYYNSNAEGDRFSWWAYPGSLAVTKGILVSFF 86
           GSEH   +  +          S A  +S      +    F+  A P SLA T GI  +FF
Sbjct: 447 GSEHKLVMLSATGLSPSRVRHSTASPSSTTLV*LSHNPVFTV*AAPISLAATTGIAFAFF 268

Query: 87  SSAY 90
           SS Y
Sbjct: 267 SSGY 256


>TC8864 
          Length = 658

 Score = 25.4 bits (54), Expect = 1.8
 Identities = 12/23 (52%), Positives = 14/23 (60%), Gaps = 2/23 (8%)
 Frame = +3

Query: 9  WGCIPKQPDS--PTTPRGATGSE 29
          WG  P   +   P+TP GATGSE
Sbjct: 54 WGSPPPTENGAGPSTPSGATGSE 122


>AU251869 
          Length = 284

 Score = 25.4 bits (54), Expect = 1.8
 Identities = 13/51 (25%), Positives = 22/51 (42%), Gaps = 10/51 (19%)
 Frame = +3

Query: 44  TWARSAAEDASP----------YYNSNAEGDRFSWWAYPGSLAVTKGILVS 84
           TWA S     SP          +++ N +G  + W  + G   +  G+L+S
Sbjct: 60  TWAASPISSESPKLEGLQKHVAFFDLNQDGVIYPWETFKGLRLIGSGVLLS 212


>TC8360 weakly similar to UP|O22940 (O22940) At2g41800 protein
           (At2g41800/T11A7.10), partial (82%)
          Length = 1366

 Score = 25.0 bits (53), Expect = 2.4
 Identities = 7/9 (77%), Positives = 9/9 (99%)
 Frame = -2

Query: 2   GRNLPPDWG 10
           G+N+PPDWG
Sbjct: 306 GKNMPPDWG 280


>TC19448 homologue to UP|O76915 (O76915) NG3 protein, partial (11%)
          Length = 593

 Score = 25.0 bits (53), Expect = 2.4
 Identities = 20/67 (29%), Positives = 28/67 (40%), Gaps = 15/67 (22%)
 Frame = -2

Query: 9   WGCIPKQPDSPTTPRGATGSEHNGALTLSGAPFQGT---------------WARSAAEDA 53
           W  +P   DSP  P       HN  L LS +P +                 W RS++ + 
Sbjct: 184 WT*LPHHWDSPKWP------SHN-TLLLSHSPNEPLSSTLRSC*EKAGSLDWLRSSSGNT 26

Query: 54  SPYYNSN 60
           SPYY+S+
Sbjct: 25  SPYYHSH 5


>BP066148 
          Length = 477

 Score = 25.0 bits (53), Expect = 2.4
 Identities = 13/29 (44%), Positives = 17/29 (57%)
 Frame = -2

Query: 33  ALTLSGAPFQGTWARSAAEDASPYYNSNA 61
           +L +S AP +GT +RS  E A     SNA
Sbjct: 458 SLEVSDAPAEGTHSRSLTERALDLIRSNA 372


>TC9953 similar to GB|CAD35362.1|21535744|ATH490171 FK506 binding protein 1
           {Arabidopsis thaliana;} , partial (29%)
          Length = 481

 Score = 25.0 bits (53), Expect = 2.4
 Identities = 12/34 (35%), Positives = 15/34 (43%)
 Frame = -2

Query: 10  GCIPKQPDSPTTPRGATGSEHNGALTLSGAPFQG 43
           G +P        P GA  + H GA T + A F G
Sbjct: 339 GPVPTSLSQKARPEGAAVNSHGGASTAAEAGFGG 238


>TC12903 weakly similar to UP|ALA9_ARATH (Q9SX33) Potential
           phospholipid-transporting ATPase 9  (Aminophospholipid
           flippase 9) , partial (14%)
          Length = 746

 Score = 24.6 bits (52), Expect = 3.1
 Identities = 10/20 (50%), Positives = 12/20 (60%)
 Frame = -3

Query: 35  TLSGAPFQGTWARSAAEDAS 54
           T S  PF GTW R+ +E  S
Sbjct: 525 TSSIVPFHGTWERTTSESLS 466


>TC8032 similar to UP|Q9M0B6 (Q9M0B6) Nucleotide sugar epimerase-like
           protein, partial (97%)
          Length = 2230

 Score = 24.6 bits (52), Expect = 3.1
 Identities = 14/42 (33%), Positives = 19/42 (44%)
 Frame = +1

Query: 14  KQPDSPTTPRGATGSEHNGALTLSGAPFQGTWARSAAEDASP 55
           K+P  P++P  A  S    +   S +P   TW RS     SP
Sbjct: 550 KKPAKPSSPPTAFSSWKATSTMPSFSPSSSTWWRSPMSCTSP 675


>TC9308 weakly similar to UP|Q9SQ55 (Q9SQ55) Nuclear RNA binding protein A,
           partial (9%)
          Length = 562

 Score = 24.6 bits (52), Expect = 3.1
 Identities = 12/29 (41%), Positives = 17/29 (58%)
 Frame = +3

Query: 33  ALTLSGAPFQGTWARSAAEDASPYYNSNA 61
           +L +  AP +GT +RS  E A  +  SNA
Sbjct: 138 SLEVPDAPAEGTHSRSLTERALDFIRSNA 224


>TC15711 weakly similar to UP|MID2_YEAST (P36027) Mating process protein
           MID2 (Serine-rich protein SMS1) (Protein kinase A
           interference protein), partial (6%)
          Length = 1014

 Score = 24.6 bits (52), Expect = 3.1
 Identities = 16/42 (38%), Positives = 21/42 (49%), Gaps = 3/42 (7%)
 Frame = +2

Query: 50  AEDASPYYNSNAEGDRFSWW--AYPGSLAVTKGILV-SFFSS 88
           AE   PY N   E  R  WW   + G +++  GI V S +SS
Sbjct: 329 AEARHPYRNDAVEDRR*RWWNKQWQGRISIHSGIGVWSCYSS 454


>TC17082 
          Length = 554

 Score = 24.6 bits (52), Expect = 3.1
 Identities = 12/38 (31%), Positives = 16/38 (41%), Gaps = 3/38 (7%)
 Frame = +3

Query: 38 GAPFQGTWARSAAEDASPYYNSNAEGDR---FSWWAYP 72
          G  F G W         P+Y    +GDR   F+W+  P
Sbjct: 54 GGFFCGRWLCPYLPGKHPFYREIVDGDRCF*FTWFQLP 167


>BP058189 
          Length = 537

 Score = 24.3 bits (51), Expect = 4.0
 Identities = 9/13 (69%), Positives = 11/13 (84%)
 Frame = -1

Query: 69  WAYPGSLAVTKGI 81
           W  PGSLAV++GI
Sbjct: 369 WRIPGSLAVSRGI 331


>TC11170 weakly similar to UP|Q9FKT4 (Q9FKT4) RNA-binding protein-like,
           partial (32%)
          Length = 613

 Score = 24.3 bits (51), Expect = 4.0
 Identities = 9/17 (52%), Positives = 12/17 (69%)
 Frame = -1

Query: 22  PRGATGSEHNGALTLSG 38
           P GA G  H G+++LSG
Sbjct: 187 PAGAGGRHHGGSISLSG 137


>TC19667 similar to UP|O24078 (O24078) Protein phosphatase 2C, partial (9%)
          Length = 591

 Score = 24.3 bits (51), Expect = 4.0
 Identities = 9/13 (69%), Positives = 11/13 (84%)
 Frame = +3

Query: 69  WAYPGSLAVTKGI 81
           W  PGSLAV++GI
Sbjct: 159 WRIPGSLAVSRGI 197


>AV767360 
          Length = 389

 Score = 24.3 bits (51), Expect = 4.0
 Identities = 12/25 (48%), Positives = 14/25 (56%)
 Frame = -2

Query: 13  PKQPDSPTTPRGATGSEHNGALTLS 37
           P +  SP +PRG  GS   GAL  S
Sbjct: 343 PPESGSPPSPRGMKGSIPPGALKKS 269


>BP057603 
          Length = 538

 Score = 23.9 bits (50), Expect = 5.3
 Identities = 12/37 (32%), Positives = 20/37 (53%)
 Frame = +1

Query: 53  ASPYYNSNAEGDRFSWWAYPGSLAVTKGILVSFFSSA 89
           A+  +NS+    RFS      S +++K +   FFSS+
Sbjct: 346 ATSPFNSSTSASRFSQVTLSTSRSISKEVTKFFFSSS 456


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.313    0.132    0.435 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 1,802,930
Number of Sequences: 28460
Number of extensions: 22606
Number of successful extensions: 121
Number of sequences better than 10.0: 53
Number of HSP's better than 10.0 without gapping: 121
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 121
length of query: 90
length of database: 4,897,600
effective HSP length: 66
effective length of query: 24
effective length of database: 3,019,240
effective search space: 72461760
effective search space used: 72461760
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 48 (23.1 bits)


Medicago: description of AC136838.2