BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136507.8 + phase: 0
(146 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC19040 homologue to UP|Q9EZV9 (Q9EZV9) Elongation factor Tu (Fr... 139 2e-34
TC10722 85 6e-18
TC9392 53 2e-08
BP050242 32 0.033
AU251457 32 0.057
BP045881 26 2.4
CB828176 26 2.4
AV770579 25 4.1
BP075835 25 5.3
TC20277 homologue to UP|Q7Y067 (Q7Y067) Plasma membrane H+-ATPas... 25 5.3
TC19343 25 5.3
TC17629 similar to UP|Q39193 (Q39193) Protein kinase (Protein ki... 25 7.0
TC11321 weakly similar to UP|Q9LKP0 (Q9LKP0) RNA-dependent RNA p... 25 7.0
AW164024 25 7.0
AV767409 24 9.1
BP078534 24 9.1
TC14287 similar to UP|Q9LEU7 (Q9LEU7) Serine/threonine protein k... 24 9.1
TC14357 similar to UP|PLAS_PEA (P16002) Plastocyanin, chloroplas... 24 9.1
>TC19040 homologue to UP|Q9EZV9 (Q9EZV9) Elongation factor Tu (Fragment),
partial (5%)
Length = 721
Score = 139 bits (350), Expect = 2e-34
Identities = 68/143 (47%), Positives = 95/143 (65%), Gaps = 1/143 (0%)
Frame = +3
Query: 1 MRPPEKRVDYKVGDKVEVCSEDEGFVGSYFEATIVSCLESGKYVIRYKNLLKDDESEL-L 59
MRPP++ V YKVGD+VEV ++EGF SY+EAT+VS L G+Y ++YK L+ D+E+++ L
Sbjct: 69 MRPPQRTVVYKVGDEVEVIGKEEGFYTSYYEATVVSLLPFGRYEVQYKTLIVDEETKIPL 248
Query: 60 METLFPKDLRPIPPHVRNPLKFKLNQKVDVFDNDGWWVGKIASEKILMEKSCYYSVYFDY 119
E + KDLRP+PP V ++KL Q V+ FDNDGWW G I +I Y+VYF
Sbjct: 249 KEAVLAKDLRPVPPLVNTRGEYKLYQAVNTFDNDGWWYGMIVG-RISCTGGKGYNVYFSS 425
Query: 120 CHQTIYYPCDQIRVHQELVWGDW 142
++T+ Y C +IRVH E +W
Sbjct: 426 TNETLPYNCSRIRVHHEFFDAEW 494
>TC10722
Length = 500
Score = 84.7 bits (208), Expect = 6e-18
Identities = 44/95 (46%), Positives = 62/95 (64%), Gaps = 2/95 (2%)
Frame = +2
Query: 50 LLKDDESEL-LMETLFPKDLRPIPPHVRNPL-KFKLNQKVDVFDNDGWWVGKIASEKILM 107
L+ D+E+++ L ET+ PKDLRP+PP VR +++ Q V+VFDNDGWW GKI L
Sbjct: 5 LIVDEETKIPLKETVLPKDLRPVPPCVRTRGGHYEMYQAVNVFDNDGWWYGKIIG---LT 175
Query: 108 EKSCYYSVYFDYCHQTIYYPCDQIRVHQELVWGDW 142
YY VYF ++T+ Y C ++RVH + + GDW
Sbjct: 176 GVKGYY-VYFSSTNETLPYHCSRLRVHHDFIHGDW 277
>TC9392
Length = 650
Score = 52.8 bits (125), Expect = 2e-08
Identities = 24/69 (34%), Positives = 44/69 (62%)
Frame = +1
Query: 5 EKRVDYKVGDKVEVCSEDEGFVGSYFEATIVSCLESGKYVIRYKNLLKDDESELLMETLF 64
+K+ +++GD VEV S+ +G+ G++F A +V GK+++ +K+ L D +LL E +
Sbjct: 442 QKQELFRIGDLVEVSSKVKGYRGTWFLAEVVELKVQGKFLVEHKHRLHDVTGKLLKEKID 621
Query: 65 PKDLRPIPP 73
+RP+PP
Sbjct: 622 DDHIRPLPP 648
Score = 35.4 bits (80), Expect = 0.004
Identities = 21/75 (28%), Positives = 35/75 (46%)
Frame = +1
Query: 68 LRPIPPHVRNPLKFKLNQKVDVFDNDGWWVGKIASEKILMEKSCYYSVYFDYCHQTIYYP 127
+RP PP + FK+ KVD +D W G + E + ++V F+ Q +P
Sbjct: 214 VRPRPPPETHH-DFKIGDKVDAYDKGSWREGHLVKEL----EDGKFAVDFNLPKQLNEFP 378
Query: 128 CDQIRVHQELVWGDW 142
+ +R H+E + W
Sbjct: 379 KENLRTHREWIDDHW 423
Score = 35.0 bits (79), Expect = 0.005
Identities = 17/45 (37%), Positives = 26/45 (57%)
Frame = +1
Query: 3 PPEKRVDYKVGDKVEVCSEDEGFVGSYFEATIVSCLESGKYVIRY 47
PPE D+K+GDKV+ + GS+ E +V LE GK+ + +
Sbjct: 229 PPETHHDFKIGDKVDAYDK-----GSWREGHLVKELEDGKFAVDF 348
>BP050242
Length = 585
Score = 32.3 bits (72), Expect = 0.033
Identities = 23/77 (29%), Positives = 40/77 (51%), Gaps = 1/77 (1%)
Frame = +1
Query: 67 DLRPIPPHVRNPLKFKLNQKVDVFDNDGWWV-GKIASEKILMEKSCYYSVYFDYCHQTIY 125
+LRP PP ++ FK KVD +++ G W+ G I S L + + V ++ +Q
Sbjct: 337 NLRPRPP-LKPHHDFKSGDKVDTYNDKGSWLEGHITS--TLSDGRSF--VCYNDPNQKFL 501
Query: 126 YPCDQIRVHQELVWGDW 142
+P +++R H E + W
Sbjct: 502 FPNEKLRAHHEWIDDHW 552
Score = 29.6 bits (65), Expect = 0.22
Identities = 18/49 (36%), Positives = 26/49 (52%), Gaps = 1/49 (2%)
Frame = +1
Query: 2 RPPEK-RVDYKVGDKVEVCSEDEGFVGSYFEATIVSCLESGKYVIRYKN 49
RPP K D+K GDKV+ ++ GS+ E I S L G+ + Y +
Sbjct: 349 RPPLKPHHDFKSGDKVDTYNDK----GSWLEGHITSTLSDGRSFVCYND 483
>AU251457
Length = 389
Score = 31.6 bits (70), Expect = 0.057
Identities = 13/32 (40%), Positives = 18/32 (55%)
Frame = +1
Query: 4 PEKRVDYKVGDKVEVCSEDEGFVGSYFEATIV 35
P YKV DK+E+ +D G G +F T+V
Sbjct: 271 PWNNPTYKVDDKIELLCQDSGIRGCWFRCTVV 366
>BP045881
Length = 420
Score = 26.2 bits (56), Expect = 2.4
Identities = 15/48 (31%), Positives = 24/48 (49%), Gaps = 1/48 (2%)
Frame = -2
Query: 53 DDESELLMETLFPKDLRPIPPHVRNPLKFKLNQKVDVF-DNDGWWVGK 99
D + TLFP+ LRP+ V + L + D F D++G+ G+
Sbjct: 392 DPHEDFAFSTLFPEFLRPVIDPVASIFHRMLCGRSDAFNDSEGYTFGR 249
>CB828176
Length = 363
Score = 26.2 bits (56), Expect = 2.4
Identities = 19/77 (24%), Positives = 31/77 (39%), Gaps = 11/77 (14%)
Frame = +3
Query: 77 NPLKFKLNQ------KVDVFDNDGWWVGKIASEKI-----LMEKSCYYSVYFDYCHQTIY 125
NP+ FKL+Q ++ D + ++S +M S Y+S Y + I
Sbjct: 6 NPIIFKLDQTLMEWEEMRTLDGATLFASFLSSHSRTELPGMMRNSVYFSKVRFYGKRCIS 185
Query: 126 YPCDQIRVHQELVWGDW 142
+ D R + W DW
Sbjct: 186 FSLDDFRYYPRKQWHDW 236
>AV770579
Length = 256
Score = 25.4 bits (54), Expect = 4.1
Identities = 10/25 (40%), Positives = 13/25 (52%)
Frame = -1
Query: 16 VEVCSEDEGFVGSYFEATIVSCLES 40
+ +C E VG F +VSCL S
Sbjct: 193 INICRETHKSVGGXFVIAVVSCLSS 119
>BP075835
Length = 379
Score = 25.0 bits (53), Expect = 5.3
Identities = 9/28 (32%), Positives = 14/28 (49%)
Frame = -3
Query: 101 ASEKILMEKSCYYSVYFDYCHQTIYYPC 128
+ + IL+ C Y + Y H +YPC
Sbjct: 290 SQKNILVRVKC*YILILTYAHSHDWYPC 207
>TC20277 homologue to UP|Q7Y067 (Q7Y067) Plasma membrane H+-ATPase, partial
(3%)
Length = 584
Score = 25.0 bits (53), Expect = 5.3
Identities = 10/19 (52%), Positives = 12/19 (62%)
Frame = -3
Query: 124 IYYPCDQIRVHQELVWGDW 142
+ +P QIRVH L W DW
Sbjct: 474 VCHPSGQIRVHHRLCW-DW 421
>TC19343
Length = 520
Score = 25.0 bits (53), Expect = 5.3
Identities = 7/16 (43%), Positives = 10/16 (61%)
Frame = -3
Query: 82 KLNQKVDVFDNDGWWV 97
+ N + VFD GWW+
Sbjct: 431 RFNNHITVFDTHGWWM 384
>TC17629 similar to UP|Q39193 (Q39193) Protein kinase (Protein kinase, 41K)
, partial (20%)
Length = 578
Score = 24.6 bits (52), Expect = 7.0
Identities = 14/43 (32%), Positives = 22/43 (50%), Gaps = 2/43 (4%)
Frame = -2
Query: 99 KIASEKIL--MEKSCYYSVYFDYCHQTIYYPCDQIRVHQELVW 139
K+ S K++ + SC+YS + +Y H D+I LVW
Sbjct: 160 KVISHKLI*RIRPSCWYSSF*NYLH-------DRINALHGLVW 53
>TC11321 weakly similar to UP|Q9LKP0 (Q9LKP0) RNA-dependent RNA polymerase,
partial (10%)
Length = 617
Score = 24.6 bits (52), Expect = 7.0
Identities = 9/18 (50%), Positives = 11/18 (61%)
Frame = +3
Query: 71 IPPHVRNPLKFKLNQKVD 88
+PPH R P +F Q VD
Sbjct: 135 LPPHTRFPFRFSHQQPVD 188
>AW164024
Length = 312
Score = 24.6 bits (52), Expect = 7.0
Identities = 14/47 (29%), Positives = 23/47 (48%)
Frame = +1
Query: 26 VGSYFEATIVSCLESGKYVIRYKNLLKDDESELLMETLFPKDLRPIP 72
+ S F A + L+ G+Y + + L DD +E L+ P P+P
Sbjct: 139 ISSVFSADMSENLDDGEYWLPPQFLSDDDATESLLPFGAPNRRPPMP 279
>AV767409
Length = 317
Score = 24.3 bits (51), Expect = 9.1
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = +3
Query: 100 IASEKILMEKSCYYSVYFDYCHQT 123
+A ++ EKSCY+ YF H++
Sbjct: 174 VAKKERCKEKSCYHHSYFRDAHRS 245
>BP078534
Length = 410
Score = 24.3 bits (51), Expect = 9.1
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -1
Query: 125 YYPCDQIRVHQELVW 139
Y PC +IRVH L+W
Sbjct: 164 YSPCVKIRVHWLLMW 120
>TC14287 similar to UP|Q9LEU7 (Q9LEU7) Serine/threonine protein kinase-like
protein (CBL-interacting protein kinase 5), partial (75%)
Length = 1999
Score = 24.3 bits (51), Expect = 9.1
Identities = 12/30 (40%), Positives = 17/30 (56%)
Frame = -3
Query: 63 LFPKDLRPIPPHVRNPLKFKLNQKVDVFDN 92
+F + PI PH PLK K+ +K + DN
Sbjct: 1085 IFSLESLPINPHHILPLKRKIPRKQSI*DN 996
>TC14357 similar to UP|PLAS_PEA (P16002) Plastocyanin, chloroplast
precursor, partial (97%)
Length = 759
Score = 24.3 bits (51), Expect = 9.1
Identities = 10/19 (52%), Positives = 13/19 (67%)
Frame = -2
Query: 53 DDESELLMETLFPKDLRPI 71
DD++ L TL P DLRP+
Sbjct: 179 DDDTLALALTLLPADLRPV 123
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.142 0.457
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 3,199,961
Number of Sequences: 28460
Number of extensions: 44537
Number of successful extensions: 264
Number of sequences better than 10.0: 38
Number of HSP's better than 10.0 without gapping: 257
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 260
length of query: 146
length of database: 4,897,600
effective HSP length: 82
effective length of query: 64
effective length of database: 2,563,880
effective search space: 164088320
effective search space used: 164088320
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)
Medicago: description of AC136507.8