BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136472.8 + phase: 0
(182 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC14249 homologue to UP|Q7XZJ2 (Q7XZJ2) Actin, partial (63%) 298 5e-82
TC14247 homologue to UP|Q9SLQ6 (Q9SLQ6) Actin isoform B, complete 296 1e-81
TC8090 homologue to UP|ACTB_ARATH (P53496) Actin 11, complete 296 1e-81
TC17038 homologue to UP|ACT1_SORBI (P53504) Actin 1, partial (63%) 295 2e-81
TC18138 UP|ACT1_TOBAC (Q05214) Actin, partial (39%) 295 4e-81
TC12003 homologue to UP|AAQ55798 (AAQ55798) Actin, partial (50%) 277 9e-76
AU240100 142 4e-35
AV420778 141 7e-35
AI967511 96 4e-21
TC17089 similar to UP|Q8L4Y5 (Q8L4Y5) Actin-related protein 7, p... 80 2e-16
TC13010 similar to UP|Q84M92 (Q84M92) At1g18450 (Actin-related p... 60 3e-10
TC18885 homologue to UP|Q96442 (Q96442) Actin (Fragment), partia... 52 8e-08
BG662336 47 3e-06
AV407640 40 3e-04
BI418387 32 0.049
BP074912 28 0.93
AV425593 26 3.5
AW719626 25 7.8
>TC14249 homologue to UP|Q7XZJ2 (Q7XZJ2) Actin, partial (63%)
Length = 916
Score = 298 bits (762), Expect = 5e-82
Identities = 144/146 (98%), Positives = 145/146 (98%)
Frame = +2
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEA 60
MADAEDIQPLV DNGTGMVKAGFAGDDAPRAVFPSI+GRPRHTGVMVGMGQKDAYVGDEA
Sbjct: 206 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 385
Query: 61 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 120
QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK
Sbjct: 386 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 565
Query: 121 MTQIMFETFNVPAMYVAIQAVLSLYA 146
MTQIMFETFNVPAMYVAIQAVLSLYA
Sbjct: 566 MTQIMFETFNVPAMYVAIQAVLSLYA 643
>TC14247 homologue to UP|Q9SLQ6 (Q9SLQ6) Actin isoform B, complete
Length = 1656
Score = 296 bits (759), Expect = 1e-81
Identities = 143/146 (97%), Positives = 145/146 (98%)
Frame = +2
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEA 60
MADAEDIQPLV DNGTGMVKAGFAGDDAPRAVFPSI+GRPRHTGVMVGMGQKD+YVGDEA
Sbjct: 146 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDSYVGDEA 325
Query: 61 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 120
QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK
Sbjct: 326 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 505
Query: 121 MTQIMFETFNVPAMYVAIQAVLSLYA 146
MTQIMFETFNVPAMYVAIQAVLSLYA
Sbjct: 506 MTQIMFETFNVPAMYVAIQAVLSLYA 583
>TC8090 homologue to UP|ACTB_ARATH (P53496) Actin 11, complete
Length = 1624
Score = 296 bits (758), Expect = 1e-81
Identities = 143/146 (97%), Positives = 144/146 (97%)
Frame = +2
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEA 60
MADAEDIQPLV DNGTGMVKAGFAGDDAPRAVFPSI+GRPRHTGVMVGMGQKDAYVGDEA
Sbjct: 98 MADAEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 277
Query: 61 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 120
QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK
Sbjct: 278 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 457
Query: 121 MTQIMFETFNVPAMYVAIQAVLSLYA 146
MTQIMFETFN PAMYVAIQAVLSLYA
Sbjct: 458 MTQIMFETFNTPAMYVAIQAVLSLYA 535
>TC17038 homologue to UP|ACT1_SORBI (P53504) Actin 1, partial (63%)
Length = 761
Score = 295 bits (756), Expect = 2e-81
Identities = 143/146 (97%), Positives = 144/146 (97%)
Frame = +2
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEA 60
MADAEDIQPLV DNGTGMVKAGFAGDDAPRAVFPSI+GRPRHTGVMVGMGQKDAYVGDEA
Sbjct: 53 MADAEDIQPLVGDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 232
Query: 61 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 120
QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK
Sbjct: 233 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 412
Query: 121 MTQIMFETFNVPAMYVAIQAVLSLYA 146
MTQIMFETFN PAMYVAIQAVLSLYA
Sbjct: 413 MTQIMFETFNAPAMYVAIQAVLSLYA 490
>TC18138 UP|ACT1_TOBAC (Q05214) Actin, partial (39%)
Length = 580
Score = 295 bits (754), Expect = 4e-81
Identities = 142/146 (97%), Positives = 143/146 (97%)
Frame = +2
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEA 60
MAD EDIQPLV DNGTGMVKAGFAGDDAPRAVFPSI+GRPRHTGVMVGMGQKDAYVGDEA
Sbjct: 143 MADGEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 322
Query: 61 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 120
QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK
Sbjct: 323 QSKRGILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREK 502
Query: 121 MTQIMFETFNVPAMYVAIQAVLSLYA 146
MTQIMFETFN PAMYVAIQAVLSLYA
Sbjct: 503 MTQIMFETFNTPAMYVAIQAVLSLYA 580
>TC12003 homologue to UP|AAQ55798 (AAQ55798) Actin, partial (50%)
Length = 612
Score = 277 bits (708), Expect = 9e-76
Identities = 132/141 (93%), Positives = 137/141 (96%)
Frame = +1
Query: 6 DIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEAQSKRG 65
++Q LVVDNG+GM KAGFAGDDAPRAVFPSI+GRPRH GVMVGMGQKDAYVGDEAQSKRG
Sbjct: 52 EVQALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDAYVGDEAQSKRG 231
Query: 66 ILTLKYPIEHGIVSNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 125
ILTLKYPIEHGIV+NWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM
Sbjct: 232 ILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIM 411
Query: 126 FETFNVPAMYVAIQAVLSLYA 146
FETFN PAMYVAIQAVLSLYA
Sbjct: 412 FETFNTPAMYVAIQAVLSLYA 474
>AU240100
Length = 276
Score = 142 bits (357), Expect = 4e-35
Identities = 68/71 (95%), Positives = 70/71 (97%)
Frame = +3
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEA 60
MAD+EDIQPLV DNGTGMVKAGFAGDDAPRAVFPSI+GRPRHTGVMVGMGQKDAYVGDEA
Sbjct: 63 MADSEDIQPLVCDNGTGMVKAGFAGDDAPRAVFPSIVGRPRHTGVMVGMGQKDAYVGDEA 242
Query: 61 QSKRGILTLKY 71
QSKRGILTLKY
Sbjct: 243 QSKRGILTLKY 275
>AV420778
Length = 263
Score = 141 bits (355), Expect = 7e-35
Identities = 62/87 (71%), Positives = 73/87 (83%)
Frame = +3
Query: 6 DIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEAQSKRG 65
DIQP+V DNG+G+ K G DAP AVFPSI+GR +H G+MVG+ +KDAYVGDEA S+ G
Sbjct: 3 DIQPIVFDNGSGVFKGGLVRYDAPHAVFPSIVGRSQHAGMMVGITRKDAYVGDEADSRSG 182
Query: 66 ILTLKYPIEHGIVSNWDDMEKIWHHTF 92
+LTLKYPIEHG+VSNWDDMEKIWHH F
Sbjct: 183LLTLKYPIEHGVVSNWDDMEKIWHHAF 263
>AI967511
Length = 375
Score = 95.9 bits (237), Expect = 4e-21
Identities = 49/79 (62%), Positives = 58/79 (73%)
Frame = +1
Query: 21 AGFAGDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEAQSKRGILTLKYPIEHGIVSN 80
A FAGDDAPRAVFPSI+G R +GVM G G KD YVGDEAQS+ G L +KYP+E GIV++
Sbjct: 142 AAFAGDDAPRAVFPSILGA-RRSGVMHGQGNKDYYVGDEAQSRCGTLNIKYPMEKGIVTD 318
Query: 81 WDDMEKIWHHTFYNELRVA 99
WD ME++ EL VA
Sbjct: 319 WDAMERVCIIPCMKELHVA 375
>TC17089 similar to UP|Q8L4Y5 (Q8L4Y5) Actin-related protein 7, partial
(35%)
Length = 584
Score = 80.1 bits (196), Expect = 2e-16
Identities = 50/143 (34%), Positives = 73/143 (50%), Gaps = 2/143 (1%)
Frame = +3
Query: 6 DIQPLVVDNGTGMVKAGFA-GDDAPRAVFPSIIGRPRHTGVMVGMGQKDAYVGDEAQSKR 64
+++ VVD G+ ++KAGFA D P + P+ Q + D + S
Sbjct: 57 EMEAAVVDVGSKLLKAGFAIPDQTPPMIIPT---------------QMKRLLDDGSDSSL 191
Query: 65 GILTLKYPIEHGIVSNWDDMEKIWHHTFYNELR-VAPEEHPVLLTEAPLNPKANREKMTQ 123
T+ P+ G V +WD +E + HH Y L E +L T+ PKAN+E++ Q
Sbjct: 192 DSFTVD-PVVRGFVRDWDAIEDLLHHVLYTGLGWEIGNEGQILFTDPLCTPKANKEQLVQ 368
Query: 124 IMFETFNVPAMYVAIQAVLSLYA 146
+MFETFN+ Y + QAVLSLYA
Sbjct: 369 LMFETFNISGFYASEQAVLSLYA 437
>TC13010 similar to UP|Q84M92 (Q84M92) At1g18450 (Actin-related protein 4),
partial (22%)
Length = 571
Score = 59.7 bits (143), Expect = 3e-10
Identities = 32/108 (29%), Positives = 56/108 (51%), Gaps = 20/108 (18%)
Frame = +3
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIGR-------------------PR 41
M +++ +V+D G+ KAG+AG+DAP+AVFPS++G
Sbjct: 246 MYGGDEVSAIVIDLGSHTCKAGYAGEDAPKAVFPSVVGAIDQMDIDEADNAEKNSASGTE 425
Query: 42 HTGVMVGMGQKDAYVGDEAQS-KRGILTLKYPIEHGIVSNWDDMEKIW 88
T G++ YVG ++ +R + + P++ GIV++WD ++ IW
Sbjct: 426 LTNSDKAKGKRKLYVGSQSLGYRRDHMEVLSPLQDGIVADWDIVDSIW 569
>TC18885 homologue to UP|Q96442 (Q96442) Actin (Fragment), partial (55%)
Length = 561
Score = 51.6 bits (122), Expect = 8e-08
Identities = 25/25 (100%), Positives = 25/25 (100%)
Frame = +3
Query: 122 TQIMFETFNVPAMYVAIQAVLSLYA 146
TQIMFETFNVPAMYVAIQAVLSLYA
Sbjct: 3 TQIMFETFNVPAMYVAIQAVLSLYA 77
>BG662336
Length = 334
Score = 46.6 bits (109), Expect = 3e-06
Identities = 18/38 (47%), Positives = 29/38 (75%)
Frame = +2
Query: 1 MADAEDIQPLVVDNGTGMVKAGFAGDDAPRAVFPSIIG 38
M +++ +V+D G+ KAG+AG+DAP+AVFPS++G
Sbjct: 134 MYGGDEVSAIVIDLGSHTCKAGYAGEDAPKAVFPSVVG 247
>AV407640
Length = 121
Score = 39.7 bits (91), Expect = 3e-04
Identities = 18/19 (94%), Positives = 18/19 (94%)
Frame = +1
Query: 1 MADAEDIQPLVVDNGTGMV 19
MADAEDIQPLV DNGTGMV
Sbjct: 64 MADAEDIQPLVCDNGTGMV 120
>BI418387
Length = 479
Score = 32.3 bits (72), Expect = 0.049
Identities = 24/93 (25%), Positives = 41/93 (43%), Gaps = 9/93 (9%)
Frame = -3
Query: 4 AEDIQPL----VVDNGTGMVKAGFAGDDAPRAVFPSIIGRPRHTGVMVG-----MGQKDA 54
AED P+ + D G G+V G G + V ++G V++G +G+
Sbjct: 438 AEDSAPVGFSDLEDEGCGLVGPGNGGSE--EVVVDGLVGAEGEDDVVLGGASEEVGEIRV 265
Query: 55 YVGDEAQSKRGILTLKYPIEHGIVSNWDDMEKI 87
G E G ++++ + HG+V WD E +
Sbjct: 264 LNGREGMQVEGEVSVEMIVLHGLVE*WDSEELV 166
>BP074912
Length = 519
Score = 28.1 bits (61), Expect = 0.93
Identities = 15/44 (34%), Positives = 23/44 (52%)
Frame = +3
Query: 96 LRVAPEEHPVLLTEAPLNPKANREKMTQIMFETFNVPAMYVAIQ 139
L+ +PE P +AP P+ K+ + + TFN P + AIQ
Sbjct: 342 LQPSPEHFPKYEQQAPAMPECFTPKLVEYLHRTFNEPQL-AAIQ 470
>AV425593
Length = 198
Score = 26.2 bits (56), Expect = 3.5
Identities = 12/34 (35%), Positives = 21/34 (61%), Gaps = 1/34 (2%)
Frame = +2
Query: 7 IQPLVVDNGTGMVKAGFA-GDDAPRAVFPSIIGR 39
++ V+D G+ ++KAGFA D P + P+ + R
Sbjct: 95 MEAAVIDAGSSLLKAGFAIPDQTPAMIIPTQMKR 196
>AW719626
Length = 598
Score = 25.0 bits (53), Expect = 7.8
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = -1
Query: 84 MEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKAN 117
M+ IW + E EE PVLL + LNP+ +
Sbjct: 214 MDLIWELEQFEE-----EEEPVLLNQEELNPETD 128
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.319 0.136 0.409
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,123,128
Number of Sequences: 28460
Number of extensions: 24676
Number of successful extensions: 139
Number of sequences better than 10.0: 36
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of query: 182
length of database: 4,897,600
effective HSP length: 85
effective length of query: 97
effective length of database: 2,478,500
effective search space: 240414500
effective search space used: 240414500
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)
Medicago: description of AC136472.8