Medicago
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= AC136286.9 - phase: 0 
         (222 letters)

Database: LJGI 
           28,460 sequences; 14,692,800 total letters

Searching..................................................done


                                                                   Score     E
Sequences producing significant alignments:                        (bits)  Value

CB828899                                                              193  2e-50
AV422054                                                              152  3e-38
TC17964 homologue to UP|Q83D73 (Q83D73) Ribosomal protein L9, pa...   135  4e-33
TC16216 similar to UP|RK9_PEA (P11894) 50S ribosomal protein L9,...    38  0.001
AV776056                                                               27  2.8
BP073073                                                               26  6.2
TC12444 similar to UP|O22981 (O22981) Ser/Thr protein kinase iso...    25  8.2
TC10823 similar to UP|O81446 (O81446) Calcineurin B-like protein...    25  8.2

>CB828899 
          Length = 491

 Score =  193 bits (491), Expect = 2e-50
 Identities = 95/113 (84%), Positives = 103/113 (91%)
 Frame = +3

Query: 1   MGYLQFGRYSVRQIVRFKDVVNEGVVVNPLMYASQGLRYNRKLQVILTTNIDKLGKAGET 60
           MGYLQ+GR  VRQI+RF+D  +   VVNPL+YASQGLRYNRKLQVILTT+IDKLGKAG+T
Sbjct: 153 MGYLQYGRLGVRQIIRFRDSSSNSAVVNPLLYASQGLRYNRKLQVILTTDIDKLGKAGDT 332

Query: 61  VKVAPGFFRNHLMPKLLAVPNIDKFAYLLTEQRKIYQPTEEVKQEDVVVVTES 113
           VKVAPG+FRNHLMPKLLAVPNIDKFAYLLTEQRKIYQPTEE KQEDV VV ES
Sbjct: 333 VKVAPGYFRNHLMPKLLAVPNIDKFAYLLTEQRKIYQPTEEEKQEDVTVVKES 491


>AV422054 
          Length = 399

 Score =  152 bits (385), Expect = 3e-38
 Identities = 73/88 (82%), Positives = 81/88 (91%)
 Frame = +1

Query: 1   MGYLQFGRYSVRQIVRFKDVVNEGVVVNPLMYASQGLRYNRKLQVILTTNIDKLGKAGET 60
           MGYLQ+GR  VRQI+RF+D  +   VVNPL+YASQGLRYNRKLQVILTT+IDKLGKAG+T
Sbjct: 136 MGYLQYGRLGVRQIIRFRDSSSNSAVVNPLLYASQGLRYNRKLQVILTTDIDKLGKAGDT 315

Query: 61  VKVAPGFFRNHLMPKLLAVPNIDKFAYL 88
           VKVAPG+FRNHLMPKLLAVPNIDKFAYL
Sbjct: 316 VKVAPGYFRNHLMPKLLAVPNIDKFAYL 399


>TC17964 homologue to UP|Q83D73 (Q83D73) Ribosomal protein L9, partial (13%)
          Length = 590

 Score =  135 bits (341), Expect = 4e-33
 Identities = 64/80 (80%), Positives = 74/80 (92%)
 Frame = +1

Query: 143 KAKARESKDEPLELQIPVSKNALVAEVARQLCVNITAENLHLPTPLSTIGEYEVPLRLPR 202
           KAKAR SK EPLE+++PV+K  LVAEVARQLCVNI  ENLHLP+PLST+GEYEVPLRLPR
Sbjct: 1   KAKARASKIEPLEIRLPVTKEVLVAEVARQLCVNIAPENLHLPSPLSTVGEYEVPLRLPR 180

Query: 203 SIPLPEGKLNWALKVKIRSK 222
           SIPLPEGK+NW+L+VK+RSK
Sbjct: 181 SIPLPEGKVNWSLQVKVRSK 240


>TC16216 similar to UP|RK9_PEA (P11894) 50S ribosomal protein L9,
           chloroplast precursor (CL13), partial (96%)
          Length = 944

 Score = 38.1 bits (87), Expect = 0.001
 Identities = 45/173 (26%), Positives = 74/173 (42%), Gaps = 3/173 (1%)
 Frame = +3

Query: 31  MYASQGLRYNRKLQVILTTNIDKLGKAGETVKVAPGFFRNHLMPK---LLAVPNIDKFAY 87
           ++A +  +  RK  +IL  ++  +GK GE + V  GF+RN L+P    LL  P + K   
Sbjct: 168 VFAQKKTKKTRK--IILKEDVVDVGKKGELLDVRAGFYRNFLLPNGHALLCTPQLLK--- 332

Query: 88  LLTEQRKIYQPTEEVKQEDVVVVTESKEDLMKEYERAALILDKAKLVLRRLIDVKKAKAR 147
                        E+K ED  +  E K  + +E ++ ALI +           V   K R
Sbjct: 333 -------------EMKVEDERIEAE-KRRVKEEAQQLALIFE----------TVGAFKVR 440

Query: 148 ESKDEPLELQIPVSKNALVAEVARQLCVNITAENLHLPTPLSTIGEYEVPLRL 200
               +  ++   V+   LV  +  QL  ++    + LP    T GEY   ++L
Sbjct: 441 RKGGKGKQIFGSVTAQDLVDIIKAQLQRDVDKRIVDLPEIRET-GEYIAEIKL 596


>AV776056 
          Length = 372

 Score = 26.9 bits (58), Expect = 2.8
 Identities = 15/31 (48%), Positives = 17/31 (54%), Gaps = 7/31 (22%)
 Frame = -3

Query: 185 PTPLSTIGEYEVPLRLPR-------SIPLPE 208
           PTP S IG +  PL LPR        IPLP+
Sbjct: 280 PTPDSEIGNFLEPLSLPRPKLKFELKIPLPD 188


>BP073073 
          Length = 490

 Score = 25.8 bits (55), Expect = 6.2
 Identities = 13/31 (41%), Positives = 18/31 (57%)
 Frame = -2

Query: 15  VRFKDVVNEGVVVNPLMYASQGLRYNRKLQV 45
           VRF D V EGVVVN +M       +  +++V
Sbjct: 285 VRF*DEVGEGVVVNMMMMMINNTIHTTRIKV 193


>TC12444 similar to UP|O22981 (O22981) Ser/Thr protein kinase isolog,
           partial (37%)
          Length = 709

 Score = 25.4 bits (54), Expect = 8.2
 Identities = 21/92 (22%), Positives = 47/92 (50%), Gaps = 9/92 (9%)
 Frame = -1

Query: 137 RLIDVKKAKARESKDEPLELQIPVSKNALVAEVARQLCVNITAENLHLPTPLSTI----- 191
           R + + + + R SK   + L+I +S++ L  +  R+     + +++ +P P ++I     
Sbjct: 694 RCMTMSQLQCRNSKRPNISLEI-ISRSRL--QYLRRHPARSSNKSVPIPLPFTSIKHGSR 524

Query: 192 ----GEYEVPLRLPRSIPLPEGKLNWALKVKI 219
                + ++P+R  ++IP     +N A+ VKI
Sbjct: 523 NTKVSKLDLPIRPKKNIPSLNIPMNVAMAVKI 428


>TC10823 similar to UP|O81446 (O81446) Calcineurin B-like protein 2
           (AT5G55990/MDA7_3), partial (36%)
          Length = 516

 Score = 25.4 bits (54), Expect = 8.2
 Identities = 13/36 (36%), Positives = 19/36 (52%), Gaps = 2/36 (5%)
 Frame = -3

Query: 188 LSTIGEYEVPLRLPRSIPL--PEGKLNWALKVKIRS 221
           L TI  +  P+R+PRS+ L  P    +W   +  RS
Sbjct: 235 LLTISNFRWPIRMPRSVNLEAPLATKSWRSGINCRS 128


  Database: LJGI
    Posted date:  Jul 30, 2004 11:16 AM
  Number of letters in database: 14,692,800
  Number of sequences in database:  28,460
  
Lambda     K      H
   0.318    0.137    0.378 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,868,885
Number of Sequences: 28460
Number of extensions: 33697
Number of successful extensions: 195
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of query: 222
length of database: 4,897,600
effective HSP length: 87
effective length of query: 135
effective length of database: 2,421,580
effective search space: 326913300
effective search space used: 326913300
frameshift window, decay const: 50,  0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)


Medicago: description of AC136286.9