
BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136286.9 - phase: 0
(222 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
CB828899 193 2e-50
AV422054 152 3e-38
TC17964 homologue to UP|Q83D73 (Q83D73) Ribosomal protein L9, pa... 135 4e-33
TC16216 similar to UP|RK9_PEA (P11894) 50S ribosomal protein L9,... 38 0.001
AV776056 27 2.8
BP073073 26 6.2
TC12444 similar to UP|O22981 (O22981) Ser/Thr protein kinase iso... 25 8.2
TC10823 similar to UP|O81446 (O81446) Calcineurin B-like protein... 25 8.2
>CB828899
Length = 491
Score = 193 bits (491), Expect = 2e-50
Identities = 95/113 (84%), Positives = 103/113 (91%)
Frame = +3
Query: 1 MGYLQFGRYSVRQIVRFKDVVNEGVVVNPLMYASQGLRYNRKLQVILTTNIDKLGKAGET 60
MGYLQ+GR VRQI+RF+D + VVNPL+YASQGLRYNRKLQVILTT+IDKLGKAG+T
Sbjct: 153 MGYLQYGRLGVRQIIRFRDSSSNSAVVNPLLYASQGLRYNRKLQVILTTDIDKLGKAGDT 332
Query: 61 VKVAPGFFRNHLMPKLLAVPNIDKFAYLLTEQRKIYQPTEEVKQEDVVVVTES 113
VKVAPG+FRNHLMPKLLAVPNIDKFAYLLTEQRKIYQPTEE KQEDV VV ES
Sbjct: 333 VKVAPGYFRNHLMPKLLAVPNIDKFAYLLTEQRKIYQPTEEEKQEDVTVVKES 491
>AV422054
Length = 399
Score = 152 bits (385), Expect = 3e-38
Identities = 73/88 (82%), Positives = 81/88 (91%)
Frame = +1
Query: 1 MGYLQFGRYSVRQIVRFKDVVNEGVVVNPLMYASQGLRYNRKLQVILTTNIDKLGKAGET 60
MGYLQ+GR VRQI+RF+D + VVNPL+YASQGLRYNRKLQVILTT+IDKLGKAG+T
Sbjct: 136 MGYLQYGRLGVRQIIRFRDSSSNSAVVNPLLYASQGLRYNRKLQVILTTDIDKLGKAGDT 315
Query: 61 VKVAPGFFRNHLMPKLLAVPNIDKFAYL 88
VKVAPG+FRNHLMPKLLAVPNIDKFAYL
Sbjct: 316 VKVAPGYFRNHLMPKLLAVPNIDKFAYL 399
>TC17964 homologue to UP|Q83D73 (Q83D73) Ribosomal protein L9, partial (13%)
Length = 590
Score = 135 bits (341), Expect = 4e-33
Identities = 64/80 (80%), Positives = 74/80 (92%)
Frame = +1
Query: 143 KAKARESKDEPLELQIPVSKNALVAEVARQLCVNITAENLHLPTPLSTIGEYEVPLRLPR 202
KAKAR SK EPLE+++PV+K LVAEVARQLCVNI ENLHLP+PLST+GEYEVPLRLPR
Sbjct: 1 KAKARASKIEPLEIRLPVTKEVLVAEVARQLCVNIAPENLHLPSPLSTVGEYEVPLRLPR 180
Query: 203 SIPLPEGKLNWALKVKIRSK 222
SIPLPEGK+NW+L+VK+RSK
Sbjct: 181 SIPLPEGKVNWSLQVKVRSK 240
>TC16216 similar to UP|RK9_PEA (P11894) 50S ribosomal protein L9,
chloroplast precursor (CL13), partial (96%)
Length = 944
Score = 38.1 bits (87), Expect = 0.001
Identities = 45/173 (26%), Positives = 74/173 (42%), Gaps = 3/173 (1%)
Frame = +3
Query: 31 MYASQGLRYNRKLQVILTTNIDKLGKAGETVKVAPGFFRNHLMPK---LLAVPNIDKFAY 87
++A + + RK +IL ++ +GK GE + V GF+RN L+P LL P + K
Sbjct: 168 VFAQKKTKKTRK--IILKEDVVDVGKKGELLDVRAGFYRNFLLPNGHALLCTPQLLK--- 332
Query: 88 LLTEQRKIYQPTEEVKQEDVVVVTESKEDLMKEYERAALILDKAKLVLRRLIDVKKAKAR 147
E+K ED + E K + +E ++ ALI + V K R
Sbjct: 333 -------------EMKVEDERIEAE-KRRVKEEAQQLALIFE----------TVGAFKVR 440
Query: 148 ESKDEPLELQIPVSKNALVAEVARQLCVNITAENLHLPTPLSTIGEYEVPLRL 200
+ ++ V+ LV + QL ++ + LP T GEY ++L
Sbjct: 441 RKGGKGKQIFGSVTAQDLVDIIKAQLQRDVDKRIVDLPEIRET-GEYIAEIKL 596
>AV776056
Length = 372
Score = 26.9 bits (58), Expect = 2.8
Identities = 15/31 (48%), Positives = 17/31 (54%), Gaps = 7/31 (22%)
Frame = -3
Query: 185 PTPLSTIGEYEVPLRLPR-------SIPLPE 208
PTP S IG + PL LPR IPLP+
Sbjct: 280 PTPDSEIGNFLEPLSLPRPKLKFELKIPLPD 188
>BP073073
Length = 490
Score = 25.8 bits (55), Expect = 6.2
Identities = 13/31 (41%), Positives = 18/31 (57%)
Frame = -2
Query: 15 VRFKDVVNEGVVVNPLMYASQGLRYNRKLQV 45
VRF D V EGVVVN +M + +++V
Sbjct: 285 VRF*DEVGEGVVVNMMMMMINNTIHTTRIKV 193
>TC12444 similar to UP|O22981 (O22981) Ser/Thr protein kinase isolog,
partial (37%)
Length = 709
Score = 25.4 bits (54), Expect = 8.2
Identities = 21/92 (22%), Positives = 47/92 (50%), Gaps = 9/92 (9%)
Frame = -1
Query: 137 RLIDVKKAKARESKDEPLELQIPVSKNALVAEVARQLCVNITAENLHLPTPLSTI----- 191
R + + + + R SK + L+I +S++ L + R+ + +++ +P P ++I
Sbjct: 694 RCMTMSQLQCRNSKRPNISLEI-ISRSRL--QYLRRHPARSSNKSVPIPLPFTSIKHGSR 524
Query: 192 ----GEYEVPLRLPRSIPLPEGKLNWALKVKI 219
+ ++P+R ++IP +N A+ VKI
Sbjct: 523 NTKVSKLDLPIRPKKNIPSLNIPMNVAMAVKI 428
>TC10823 similar to UP|O81446 (O81446) Calcineurin B-like protein 2
(AT5G55990/MDA7_3), partial (36%)
Length = 516
Score = 25.4 bits (54), Expect = 8.2
Identities = 13/36 (36%), Positives = 19/36 (52%), Gaps = 2/36 (5%)
Frame = -3
Query: 188 LSTIGEYEVPLRLPRSIPL--PEGKLNWALKVKIRS 221
L TI + P+R+PRS+ L P +W + RS
Sbjct: 235 LLTISNFRWPIRMPRSVNLEAPLATKSWRSGINCRS 128
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.318 0.137 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,868,885
Number of Sequences: 28460
Number of extensions: 33697
Number of successful extensions: 195
Number of sequences better than 10.0: 16
Number of HSP's better than 10.0 without gapping: 194
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 195
length of query: 222
length of database: 4,897,600
effective HSP length: 87
effective length of query: 135
effective length of database: 2,421,580
effective search space: 326913300
effective search space used: 326913300
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)
Medicago: description of AC136286.9