BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136286.14 - phase: 0
(159 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
BI417786 139 3e-34
TC18562 homologue to UP|Q9FVA6 (Q9FVA6) Glu-tRNA(Gln) amidotrans... 36 0.003
TC9175 weakly similar to UP|INAD_DROME (Q24008) Inactivation-no-... 34 0.010
TC14685 weakly similar to UP|Q43383 (Q43383) 2A6 protein, partia... 34 0.014
TC19351 similar to UP|H2A_CICAR (O65759) Histone H2A, partial (98%) 33 0.023
BP043764 33 0.030
TC15655 similar to UP|Q8LE99 (Q8LE99) Transketolase-like protein... 32 0.067
AU251825 31 0.088
TC17803 31 0.088
TC19378 weakly similar to UP|O80967 (O80967) At2g39140 protein, ... 31 0.11
BI420048 30 0.15
TC10129 weakly similar to UP|Q7T9Y7 (Q7T9Y7) Odv-e66, partial (10%) 30 0.15
TC9301 similar to UP|Q7Y0Z0 (Q7Y0Z0) TPR1, complete 30 0.15
AW720377 30 0.15
TC17878 similar to GB|AAK91488.1|15215889|AY050475 AT5g02770/F9G... 30 0.20
CB826761 30 0.20
AV424897 30 0.20
BI420016 30 0.20
BP044185 30 0.25
BP044109 30 0.25
>BI417786
Length = 513
Score = 139 bits (349), Expect = 3e-34
Identities = 83/138 (60%), Positives = 93/138 (67%), Gaps = 1/138 (0%)
Frame = +1
Query: 10 TLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEP 69
+LTL+ TP P S +L L + RR +P VEE Q T EPEP
Sbjct: 166 SLTLTTSRTPKPQSL------------ALTPLPMRRRMPVPGVVEENQQST-----EPEP 294
Query: 70 KPELNESELASELKKAMQERKEQEG-NNFWNGVVSEIGEIEWPEFGKVLGTTGVVLSVIF 128
ESELASELKKAMQERK++EG +NFWNGV EI EIEWP FGKVLGTTGVVLSVIF
Sbjct: 295 -----ESELASELKKAMQERKDKEGGDNFWNGVAQEINEIEWPAFGKVLGTTGVVLSVIF 459
Query: 129 GSSAVLLTLNAILAELSD 146
GSS VLLT+NA+LAELSD
Sbjct: 460 GSSVVLLTVNAVLAELSD 513
>TC18562 homologue to UP|Q9FVA6 (Q9FVA6) Glu-tRNA(Gln) amidotransferase
subunit A, partial (4%)
Length = 239
Score = 36.2 bits (82), Expect = 0.003
Identities = 24/56 (42%), Positives = 29/56 (50%), Gaps = 3/56 (5%)
Frame = +3
Query: 15 PHFTPSPPST--LSPQFFNFKLKPSLPSLTLTRRCT-LPRAVEEKDQPTSNPEPEP 67
PHF P PS SP F L+P PSL+L T LP + PT+NP+P P
Sbjct: 45 PHFKPLAPSLSHASPSF----LQPQTPSLSLPPPSTSLPPLSLKLPSPTTNPQPPP 200
>TC9175 weakly similar to UP|INAD_DROME (Q24008)
Inactivation-no-after-potential D protein, partial (5%)
Length = 692
Score = 34.3 bits (77), Expect = 0.010
Identities = 25/75 (33%), Positives = 34/75 (45%), Gaps = 10/75 (13%)
Frame = +2
Query: 51 RAVEEKDQPTSNPEPEPEPKPELNESELAS----------ELKKAMQERKEQEGNNFWNG 100
R +++ + PEPEPE K E E E + E +KA +E K++EG N
Sbjct: 23 RRTKKQAKIVPQPEPEPEKKEEGKEEEKPAAEEGKPEEKKEEEKAAEEAKKEEGGEEKND 202
Query: 101 VVSEIGEIEWPEFGK 115
E G E E GK
Sbjct: 203 NKEEKGGEEGKEEGK 247
>TC14685 weakly similar to UP|Q43383 (Q43383) 2A6 protein, partial (38%)
Length = 1323
Score = 33.9 bits (76), Expect = 0.014
Identities = 27/95 (28%), Positives = 36/95 (37%), Gaps = 27/95 (28%)
Frame = +2
Query: 9 FTLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRA---------------- 52
FT+ + P SP + S Q + + PS PS T RR T P+
Sbjct: 29 FTVFILPTPNESPHANTS-QCLHRRWPPSPPSPTQLRRSTAPKPSRNSTSPRPESRASSI 205
Query: 53 -----------VEEKDQPTSNPEPEPEPKPELNES 76
+K PTSNP+P P P P+ S
Sbjct: 206 PESRPSPLSSFTRQKPSPTSNPDPNPIPNPKSQPS 310
>TC19351 similar to UP|H2A_CICAR (O65759) Histone H2A, partial (98%)
Length = 626
Score = 33.1 bits (74), Expect = 0.023
Identities = 23/53 (43%), Positives = 25/53 (46%), Gaps = 1/53 (1%)
Frame = +3
Query: 29 FFNFK-LKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNESELAS 80
F FK LKP LP L PR EE + NP P+P PKP N AS
Sbjct: 15 FSKFKFLKPCLPQKLLPP----PRRAEEANPKPPNPSPDP-PKPASNSPSAAS 158
>BP043764
Length = 479
Score = 32.7 bits (73), Expect = 0.030
Identities = 28/92 (30%), Positives = 36/92 (38%)
Frame = +3
Query: 6 KTSFTLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEP 65
KT+ T +P TP P +P K P S LT + L + P P P
Sbjct: 168 KTTTTPPSNPKPTPKPKPKPAPTSNPLK-NPFKSSHALTNKLWLTSKLSPPPPPPPPPPP 344
Query: 66 EPEPKPELNESELASELKKAMQERKEQEGNNF 97
P P PE E+E+A + R Q G F
Sbjct: 345 PPLPPPE-RETEIAEDKGADDVNRFRQPGKIF 437
Score = 28.1 bits (61), Expect = 0.74
Identities = 22/67 (32%), Positives = 29/67 (42%), Gaps = 5/67 (7%)
Frame = +3
Query: 11 LTLSPHFT----PSPPSTLS-PQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEP 65
L L PH T P P L + LKP P + R ++ P SNP+P
Sbjct: 30 LFLIPHLTLIHSPQTPLFLRHTTTVSSSLKP--PPDSNNSAAPFRRPLKTTTTPPSNPKP 203
Query: 66 EPEPKPE 72
P+PKP+
Sbjct: 204TPKPKPK 224
>TC15655 similar to UP|Q8LE99 (Q8LE99) Transketolase-like protein, partial
(17%)
Length = 575
Score = 31.6 bits (70), Expect = 0.067
Identities = 25/70 (35%), Positives = 30/70 (42%), Gaps = 5/70 (7%)
Frame = +3
Query: 9 FTLTLSPH----FTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTS-NP 63
F+L+LS H PP +LSP+ + PS P PRA P S NP
Sbjct: 3 FSLSLSLHQPWLLPQPPPPSLSPKPSSRAPLPSPP----------PRASPSPPSPASANP 152
Query: 64 EPEPEPKPEL 73
P P P P L
Sbjct: 153PPSPSPSPTL 182
>AU251825
Length = 237
Score = 31.2 bits (69), Expect = 0.088
Identities = 21/58 (36%), Positives = 23/58 (39%)
Frame = -1
Query: 32 FKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPELNESELASELKKAMQER 89
F S S + RR LP S+P P P P P L S LAS L ER
Sbjct: 174 FPRSSSTSSSSCHRRHRLPSLGSSSPPGVSSPPPRPSPPPPLAFSPLASCLPLPFSER 1
>TC17803
Length = 485
Score = 31.2 bits (69), Expect = 0.088
Identities = 18/58 (31%), Positives = 21/58 (36%)
Frame = -3
Query: 14 SPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKP 71
SP TP PP T P FN + K PT P+P P+P P
Sbjct: 153 SPTPTPQPPPTPRPNKFN--------------------GIPPKPHPTPKPQPTPKPNP 40
Score = 25.8 bits (55), Expect = 3.7
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -3
Query: 46 RCTLPRAVEEKDQPTSNPEPEPEPKPELNE 75
R LP+A E P+ P P+P P P N+
Sbjct: 192 RYLLPKA-EPNPHPSPTPTPQPPPTPRPNK 106
>TC19378 weakly similar to UP|O80967 (O80967) At2g39140 protein, partial
(17%)
Length = 466
Score = 30.8 bits (68), Expect = 0.11
Identities = 19/57 (33%), Positives = 24/57 (41%), Gaps = 2/57 (3%)
Frame = +1
Query: 17 FTPSPPSTLSPQFFN--FKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKP 71
FT S TL+P + L PSL + T P ++ P NP P P P P
Sbjct: 67 FTASQSHTLTPSLLHSALSLVSHAPSLLPPQSSTSP-SLPPNPNPNPNPNPNPNPNP 234
Score = 30.4 bits (67), Expect = 0.15
Identities = 24/87 (27%), Positives = 42/87 (47%), Gaps = 5/87 (5%)
Frame = +3
Query: 1 MVLQAKTSFTLTLSPH-FTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRA----VEE 55
MV+ + T LS H FT S +L+ F +LP LT + + +
Sbjct: 24 MVVASSTMAMTALSLHCFTKSHSHSLTASF------RTLPRLTCSFSSSSSKFNISFAPS 185
Query: 56 KDQPTSNPEPEPEPKPELNESELASEL 82
K +P P+P+P+P+P+ +++ A +L
Sbjct: 186 KPKPKPKPKPKPKPEPDNDDTADAGQL 266
>BI420048
Length = 584
Score = 30.4 bits (67), Expect = 0.15
Identities = 18/51 (35%), Positives = 25/51 (48%), Gaps = 5/51 (9%)
Frame = -1
Query: 27 PQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPE-----PEPKPE 72
PQ +F PS P+L +R C+ E +P NP PE P+P P+
Sbjct: 368 PQDASFPASPSQPALGASRTCS-----PEPPRPYHNPPPETQGATPQPAPK 231
>TC10129 weakly similar to UP|Q7T9Y7 (Q7T9Y7) Odv-e66, partial (10%)
Length = 562
Score = 30.4 bits (67), Expect = 0.15
Identities = 18/57 (31%), Positives = 23/57 (39%)
Frame = +1
Query: 15 PHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKP 71
P +TP+PP T SP P +PS P + QP + P P P P
Sbjct: 403 PPYTPTPPKTPSPT----NQPPHIPS--------PPNSSSPTSQPPNTPSPPKTPSP 537
Score = 26.9 bits (58), Expect = 1.7
Identities = 21/61 (34%), Positives = 23/61 (37%), Gaps = 4/61 (6%)
Frame = +1
Query: 15 PHFTPSPPSTLSP--QFFNFKLKPSLPS--LTLTRRCTLPRAVEEKDQPTSNPEPEPEPK 70
P +TP+PP T SP Q L P PS T P QP P P P
Sbjct: 163 PPYTPTPPKTPSPTSQPPYIPLPPKTPSPISQPPHVPTPPSTPSPISQPPYTPTPPKTPS 342
Query: 71 P 71
P
Sbjct: 343 P 345
>TC9301 similar to UP|Q7Y0Z0 (Q7Y0Z0) TPR1, complete
Length = 1288
Score = 30.4 bits (67), Expect = 0.15
Identities = 14/34 (41%), Positives = 18/34 (52%)
Frame = +3
Query: 58 QPTSNPEPEPEPKPELNESELASELKKAMQERKE 91
+P S PEPEPE +E A + K Q+ KE
Sbjct: 30 EPVSQPEPEPEAMEVADEESEAKQRKVEAQKEKE 131
Score = 27.7 bits (60), Expect = 0.97
Identities = 14/45 (31%), Positives = 24/45 (53%), Gaps = 2/45 (4%)
Frame = +3
Query: 54 EEKDQPTSNPEPEPEPKPELNE--SELASELKKAMQERKEQEGNN 96
E K + P +PEP+PE E E + ++ ++ +KE+E N
Sbjct: 6 ERKRAAEAEPVSQPEPEPEAMEVADEESEAKQRKVEAQKEKEAGN 140
>AW720377
Length = 597
Score = 30.4 bits (67), Expect = 0.15
Identities = 24/78 (30%), Positives = 33/78 (41%), Gaps = 8/78 (10%)
Frame = +1
Query: 4 QAKTSFTLTLSPHFTPSPP--STLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTS 61
Q +T T+ SP+ P PP ST P + + + P+ T+ P V+ S
Sbjct: 280 QTQTLNTVLPSPYPPPPPPPPSTSYPTHYPYYPQNPNPAATVVEPVLNPPGVDSYAPLNS 459
Query: 62 NP------EPEPEPKPEL 73
NP EP P P P L
Sbjct: 460 NPVSHLGYEPIPIPNPNL 513
>TC17878 similar to GB|AAK91488.1|15215889|AY050475 AT5g02770/F9G14_80
{Arabidopsis thaliana;}, partial (17%)
Length = 465
Score = 30.0 bits (66), Expect = 0.20
Identities = 28/95 (29%), Positives = 45/95 (46%), Gaps = 1/95 (1%)
Frame = +2
Query: 1 MVLQAKTSFTLTLSPHFTPSPPSTLSPQFF-NFKLKPSLPSLTLTRRCTLPRAVEEKDQP 59
+VL + SF ++ SP T Q N L+P + T + P++ + D
Sbjct: 17 VVLNSILSFIFSVLSPMASSPNDTTPTQENPNKTLEPEEEAEEPTPPQSEPQS--QPDPI 190
Query: 60 TSNPEPEPEPKPELNESELASELKKAMQERKEQEG 94
+ PE EPEP+PE S+++K M+ R E+ G
Sbjct: 191 AAEPETEPEPEPEPASDAPLSDVQKKMR-RAERFG 292
>CB826761
Length = 417
Score = 30.0 bits (66), Expect = 0.20
Identities = 16/46 (34%), Positives = 22/46 (47%), Gaps = 7/46 (15%)
Frame = +3
Query: 46 RCTLPRAVEEKDQP-------TSNPEPEPEPKPELNESELASELKK 84
RCT V DQP S+P P+P P P ++E+ S+ K
Sbjct: 6 RCTFCHVVYHLDQPPRRRSPCVSSPLPDPTPPPPSGKNEIPSDPSK 143
>AV424897
Length = 402
Score = 30.0 bits (66), Expect = 0.20
Identities = 12/23 (52%), Positives = 16/23 (69%)
Frame = +2
Query: 51 RAVEEKDQPTSNPEPEPEPKPEL 73
+A +EK+ S PEPEPEP E+
Sbjct: 161 KASKEKENEPSGPEPEPEPTTEV 229
>BI420016
Length = 559
Score = 30.0 bits (66), Expect = 0.20
Identities = 24/68 (35%), Positives = 27/68 (39%), Gaps = 2/68 (2%)
Frame = +2
Query: 15 PHFTPSPPS--TLSPQFFNFKLKPSLPSLTLTRRCTLPRAVEEKDQPTSNPEPEPEPKPE 72
P TPSPPS TL+P PS PS T P + P + P P P P P
Sbjct: 227 PASTPSPPSSPTLTPP-------PSPPSPTPNA----PLSPSSPPSPPTPPSPPPSPAPN 373
Query: 73 LNESELAS 80
S S
Sbjct: 374 PTPSATGS 397
>BP044185
Length = 575
Score = 29.6 bits (65), Expect = 0.25
Identities = 24/68 (35%), Positives = 32/68 (46%), Gaps = 11/68 (16%)
Frame = -1
Query: 15 PHFTPSPPST---LSPQFFNFKL-------KPSL-PSLTLTRRCTLPRAVEEKDQPTSNP 63
P TP P T L+P FF +L K S+ P + L + A + K +PT P
Sbjct: 359 PKPTPHPIPTSIFLAPNFFKEELTCLETPEKASVAPLVALITSFNIGIAPKFKGRPTPIP 180
Query: 64 EPEPEPKP 71
P+P PKP
Sbjct: 179 TPQPIPKP 156
>BP044109
Length = 497
Score = 29.6 bits (65), Expect = 0.25
Identities = 27/80 (33%), Positives = 34/80 (41%), Gaps = 6/80 (7%)
Frame = +2
Query: 7 TSFTLTLSPHFTPSPPSTLSPQFFNFKLKPSLPSLTLTRRCTLPRA---VEEKDQPTSNP 63
+S T S HF PSPP P N K PS S ++ + PR PTS+P
Sbjct: 68 SSSTSLSSSHFFPSPPPPPPPPNKN-KTTPSHASNATSK--STPRTQTPTTPPPSPTSSP 238
Query: 64 EPEPE---PKPELNESELAS 80
+P P KP + E S
Sbjct: 239 KPPPSTFATKPSTSPPETPS 298
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.312 0.132 0.377
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 2,838,714
Number of Sequences: 28460
Number of extensions: 47350
Number of successful extensions: 805
Number of sequences better than 10.0: 171
Number of HSP's better than 10.0 without gapping: 636
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 731
length of query: 159
length of database: 4,897,600
effective HSP length: 83
effective length of query: 76
effective length of database: 2,535,420
effective search space: 192691920
effective search space used: 192691920
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 51 (24.3 bits)
Medicago: description of AC136286.14