BLAST2 result
TBLASTN 2.2.2 [Dec-14-2001]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= AC136286.13 + phase: 0
(373 letters)
Database: LJGI
28,460 sequences; 14,692,800 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
TC8098 similar to UP|Q94FT5 (Q94FT5) Pectate lyase (Fragment), p... 235 1e-62
AU089551 203 3e-53
BP029617 189 8e-49
BP072134 153 4e-38
TC11376 similar to GB|AAM61400.1|21537059|AY084835 pectate lyase... 150 3e-37
AI967809 137 2e-33
TC17017 similar to UP|PEL8_ARATH (Q9M8Z8) Probable pectate lyase... 137 4e-33
BP047388 114 3e-26
AV770870 104 2e-23
TC9969 similar to UP|Q93XJ1 (Q93XJ1) Pectate lyase, partial (22%) 64 9e-14
TC13897 54 5e-08
AV427683 28 2.5
BP051361 28 3.2
BP065090 27 7.2
AV414055 26 9.4
AV775187 26 9.4
>TC8098 similar to UP|Q94FT5 (Q94FT5) Pectate lyase (Fragment), partial
(56%)
Length = 1349
Score = 235 bits (599), Expect = 1e-62
Identities = 113/206 (54%), Positives = 145/206 (69%)
Frame = +3
Query: 168 ISDGDGISIFGSSHIWIDHVSMRNCRDGLIDAIMGSTAITISNSHFTDHNEVMLFGASDT 227
++DGD ISIFGSSHIW DH S+ + DGL DA+MGSTAITISN+H T HNE +L G SD+
Sbjct: 3 MADGDAISIFGSSHIWDDHNSLPHRADGLADAVMGSTAITISNNHLTHHNEAILLGHSDS 182
Query: 228 YDGDQKMQITVVFNRFGKKLIQRMPRCRFGFIHVLNNFYNRWEMYAIGGTMHPTIISEGN 287
Y D++MQ+T+ +N FG+ LIQRMPRC G+ HV+NN Y WEMYAIGG+ +PTI S+GN
Sbjct: 183 YTRDKQMQVTIAYNHFGEGLIQRMPRCIHGYFHVVNNDYTHWEMYAIGGSGNPTINSQGN 362
Query: 288 KFIAPNNGHAKEITKRTLVPEAEWKTWQWRSINDLYLNGAFFRQSGAELINRPFSNKDMI 347
++ AP N AKE+TKR + EWK W WRS DL LNGA+F SGA + ++ +
Sbjct: 363 RYNAPLNPFAKEVTKRVETAQTEWKGWNWRSEGDLLLNGAYFTPSGAG-ASASYARASSL 539
Query: 348 KAKPGSYVGRLTRYSRSLRCRVGKPC 373
AK S VG +T + +L CR G+ C
Sbjct: 540 GAKSSSMVGTMTSNAGALGCRRGRSC 617
>AU089551
Length = 599
Score = 203 bits (517), Expect = 3e-53
Identities = 91/149 (61%), Positives = 117/149 (78%)
Frame = +3
Query: 14 GLGNSSCMATNPIDRCWRCDPNWANNRKKLADCVQGFGRKTTGGKDGPIYVVTDPSDSDL 73
G CMATNPIDRCWRC NWA +R+ LA C +GFGR+ GG GPIYVVTD SD+D+
Sbjct: 153 GRXGRKCMATNPIDRCWRCRKNWAQDRQLLAKCGKGFGRRAVGGLGGPIYVVTDDSDNDM 332
Query: 74 VNPRPGTLRHAVTRNGPLWIIFARSMKIRLNQELIMAGNKTIDGRGADVTIANGAGITIQ 133
V+P+PGTLR+AV + GPLWIIFAR M I L QEL+++ +KTIDGRGA+V I GAG+T+Q
Sbjct: 333 VSPKPGTLRYAVVQKGPLWIIFARXMVITLQQELLISSDKTIDGRGANVQIRGGAGLTMQ 512
Query: 134 FIENVIIHGIKIYDIMVGSGGLVRDSEDH 162
F+ NVI+HGI++ +I +GG++RDS +H
Sbjct: 513 FVNNVIVHGIRVKNIKARNGGMIRDSYNH 599
>BP029617
Length = 493
Score = 189 bits (479), Expect = 8e-49
Identities = 93/164 (56%), Positives = 113/164 (68%)
Frame = -1
Query: 80 TLRHAVTRNGPLWIIFARSMKIRLNQELIMAGNKTIDGRGADVTIANGAGITIQFIENVI 139
TLRHAV ++ PLWI+FA M I L ELI KT+DGRGA+V I IT+Q+I NVI
Sbjct: 493 TLRHAVIQDEPLWIVFAADMTINLRHELIFNSYKTVDGRGANVQITGHGCITLQYISNVI 314
Query: 140 IHGIKIYDIMVGSGGLVRDSEDHYGLRTISDGDGISIFGSSHIWIDHVSMRNCRDGLIDA 199
IH I ++ +R S H G R +SDGDGISIFG+ +IWIDH S+ C DGLIDA
Sbjct: 313 IHNIHVHHCKPSGNTNIRASPTHVGWRGVSDGDGISIFGARNIWIDHCSLSYCADGLIDA 134
Query: 200 IMGSTAITISNSHFTDHNEVMLFGASDTYDGDQKMQITVVFNRF 243
IMGSTAITISN+HF H+EVML G +D Y D+ MQ+T+ FN F
Sbjct: 133 IMGSTAITISNNHFAHHDEVMLLGHNDKYTPDRGMQVTIAFNHF 2
>BP072134
Length = 397
Score = 153 bits (387), Expect = 4e-38
Identities = 72/113 (63%), Positives = 81/113 (70%)
Frame = +2
Query: 15 LGNSSCMATNPIDRCWRCDPNWANNRKKLADCVQGFGRKTTGGKDGPIYVVTDPSDSDLV 74
LG SC NPID CWRCDPNW NNR++LADC GFG+ GGK+G IYVVTD D D V
Sbjct: 53 LGYLSCGTGNPIDDCWRCDPNWENNRQRLADCAIGFGKDALGGKNGQIYVVTDSGDDDPV 232
Query: 75 NPRPGTLRHAVTRNGPLWIIFARSMKIRLNQELIMAGNKTIDGRGADVTIANG 127
NPRPGTLR+ ++ PLWIIF R M I L QEL++ KTIDGRGA V IA G
Sbjct: 233 NPRPGTLRYGAIQDEPLWIIFKRDMVINLKQELLVNSFKTIDGRGASVHIAGG 391
>TC11376 similar to GB|AAM61400.1|21537059|AY084835 pectate lyase-like
protein {Arabidopsis thaliana;} , partial (26%)
Length = 636
Score = 150 bits (380), Expect = 3e-37
Identities = 67/107 (62%), Positives = 83/107 (76%)
Frame = +1
Query: 18 SSCMATNPIDRCWRCDPNWANNRKKLADCVQGFGRKTTGGKDGPIYVVTDPSDSDLVNPR 77
S+C NPID CW+CDP+W NNR++LADCV GFG+ GGK G Y+VTD SD+D+VNP+
Sbjct: 316 SACATGNPIDDCWKCDPDWPNNRQRLADCVIGFGQYAKGGKGGEFYLVTDSSDNDVVNPK 495
Query: 78 PGTLRHAVTRNGPLWIIFARSMKIRLNQELIMAGNKTIDGRGADVTI 124
PGTLR+ V +N PLWI+F +M I+L+QELI KTIDGRGADV I
Sbjct: 496 PGTLRYGVIQNEPLWIVFPSNMMIKLSQELIFNSYKTIDGRGADVHI 636
>AI967809
Length = 482
Score = 137 bits (346), Expect = 2e-33
Identities = 62/128 (48%), Positives = 86/128 (66%)
Frame = +2
Query: 246 KLIQRMPRCRFGFIHVLNNFYNRWEMYAIGGTMHPTIISEGNKFIAPNNGHAKEITKRTL 305
K + + RCR G+ HV+NN Y WEMYAIGG+ +PTI S+ N+F+AP+N +KE+TK
Sbjct: 44 KALSKNARCRHGYFHVVNNDYTHWEMYAIGGSANPTINSQANRFLAPDNRFSKEVTKHED 223
Query: 306 VPEAEWKTWQWRSINDLYLNGAFFRQSGAELINRPFSNKDMIKAKPGSYVGRLTRYSRSL 365
P++EWK+W WRS DL+LNGAFFR SGA + ++ + A+P S VG +T + +L
Sbjct: 224 APDSEWKSWNWRSEGDLFLNGAFFRSSGAG-ASSMYARASSLSARPSSLVGSITVTAGTL 400
Query: 366 RCRVGKPC 373
CR G C
Sbjct: 401 NCRKGSRC 424
>TC17017 similar to UP|PEL8_ARATH (Q9M8Z8) Probable pectate lyase 8
precursor , partial (28%)
Length = 670
Score = 137 bits (344), Expect = 4e-33
Identities = 63/94 (67%), Positives = 68/94 (72%)
Frame = +3
Query: 15 LGNSSCMATNPIDRCWRCDPNWANNRKKLADCVQGFGRKTTGGKDGPIYVVTDPSDSDLV 74
LG SC NPID CWRCDPNW NRK+LADC GFGR GG+DG YVVTD D D V
Sbjct: 387 LGYFSCGTGNPIDDCWRCDPNWQQNRKRLADCGIGFGRNAIGGRDGKFYVVTDSRDDDPV 566
Query: 75 NPRPGTLRHAVTRNGPLWIIFARSMKIRLNQELI 108
NPRPGTLRHAV ++ PLWI+F R M I L QELI
Sbjct: 567 NPRPGTLRHAVIQDRPLWIVFKRDMVITLKQELI 668
>BP047388
Length = 590
Score = 114 bits (284), Expect = 3e-26
Identities = 55/91 (60%), Positives = 67/91 (73%)
Frame = -2
Query: 56 GGKDGPIYVVTDPSDSDLVNPRPGTLRHAVTRNGPLWIIFARSMKIRLNQELIMAGNKTI 115
GGK Y+VTD SD D VNP+PGTLR+AV +N PLWI+F +M I+L+QELI KTI
Sbjct: 586 GGKGVQFYIVTDSSDHDPVNPKPGTLRYAVIQNEPLWIVFPSNMMIKLSQELIFNSYKTI 407
Query: 116 DGRGADVTIANGAGITIQFIENVIIHGIKIY 146
DGRGADV I G IT+Q+I NVIIH I ++
Sbjct: 406 DGRGADVHIVGGGCITLQYISNVIIHNIHVH 314
>AV770870
Length = 528
Score = 104 bits (260), Expect = 2e-23
Identities = 67/179 (37%), Positives = 93/179 (51%), Gaps = 1/179 (0%)
Frame = +2
Query: 41 KKLADCVQGFGRKTTGGKDGPIYVVTDPSDSDLVNPRPGTLRHAVTRNGPLWIIFARSMK 100
+ LA +GFGR GG GP+Y+VT +D PG+LR R PLWI+F S
Sbjct: 62 RALAGQAEGFGRNAIGGLHGPLYLVTTLADDG-----PGSLREGCRRKEPLWIVFQVSGT 226
Query: 101 IRLNQELIMAGNKTIDGRGADVTIANGAGITIQFIENVIIHGIKIYDIMVGSGGLVRDSE 160
I L L ++ KT+DGRG + + G G+ ++ E++I+ +++ GG D
Sbjct: 227 IHLQSYLSVSSYKTVDGRGQRIKLT-GKGLRLKECEHIIVCNLEL------EGGRGHDV- 382
Query: 161 DHYGLRTISDGDGISIFGSS-HIWIDHVSMRNCRDGLIDAIMGSTAITISNSHFTDHNE 218
DGI I +S HIWID S+R+ DGLID ST ITIS HF H++
Sbjct: 383 -----------DGIQIKPNSRHIWIDRCSLRDYDDGLIDITRQSTDITISRCHFASHDK 526
>TC9969 similar to UP|Q93XJ1 (Q93XJ1) Pectate lyase, partial (22%)
Length = 485
Score = 64.3 bits (155), Expect(2) = 9e-14
Identities = 29/57 (50%), Positives = 39/57 (67%), Gaps = 1/57 (1%)
Frame = +1
Query: 274 IGGTMHPTIISEGNKFIAPNNGHAKEITKR-TLVPEAEWKTWQWRSINDLYLNGAFF 329
IGG+ +PT+ S+G+ F+APN+ ++E+TK AEWK W WRS DL LNGA F
Sbjct: 1 IGGSANPTLHSQGHSFVAPNDRFSQEVTKH*GCTTRAEWKGWNWRSEGDLLLNGALF 171
Score = 28.5 bits (62), Expect(2) = 9e-14
Identities = 14/46 (30%), Positives = 24/46 (51%)
Frame = +2
Query: 328 FFRQSGAELINRPFSNKDMIKAKPGSYVGRLTRYSRSLRCRVGKPC 373
FF +GA + ++ + A+P + VG +T + +L CR G C
Sbjct: 167 FFTATGAGA-SGSYARASSLSARPSTLVGTITTGAGALNCRKGSHC 301
>TC13897
Length = 428
Score = 53.5 bits (127), Expect = 5e-08
Identities = 19/28 (67%), Positives = 23/28 (81%)
Frame = +2
Query: 19 SCMATNPIDRCWRCDPNWANNRKKLADC 46
SC+ NP+D CWRCD NWA NR+KLA+C
Sbjct: 344 SCLTGNPVDDCWRCDTNWAANRQKLAEC 427
>AV427683
Length = 308
Score = 28.1 bits (61), Expect = 2.5
Identities = 18/45 (40%), Positives = 23/45 (51%), Gaps = 4/45 (8%)
Frame = -1
Query: 311 WKTWQWRSINDLYLN---GAFFRQSGAELINRPFSNKD-MIKAKP 351
W +RS N +L G+FFR A L+N+P D MI KP
Sbjct: 293 WSLATFRSSNMNFLKVGLGSFFRIG*ASLLNKPILESDPMISPKP 159
>BP051361
Length = 458
Score = 27.7 bits (60), Expect = 3.2
Identities = 10/17 (58%), Positives = 12/17 (69%)
Frame = -2
Query: 183 WIDHVSMRNCRDGLIDA 199
WI+HV+ NC DG DA
Sbjct: 388 WIEHVTTYNCNDGPCDA 338
>BP065090
Length = 467
Score = 26.6 bits (57), Expect = 7.2
Identities = 12/29 (41%), Positives = 16/29 (54%)
Frame = -1
Query: 6 INGRRNLVGLGNSSCMATNPIDRCWRCDP 34
+ RR+LV + S +NP RCW DP
Sbjct: 188 VQRRRSLVSAADHS---SNPPSRCWETDP 111
>AV414055
Length = 431
Score = 26.2 bits (56), Expect = 9.4
Identities = 12/37 (32%), Positives = 19/37 (50%)
Frame = +1
Query: 18 SSCMATNPIDRCWRCDPNWANNRKKLADCVQGFGRKT 54
S CM ++ + RC RC + RK+ + +G KT
Sbjct: 94 SGCMPSSRVPRC*RCS*GYCEQRKRDRNAERGSFVKT 204
>AV775187
Length = 495
Score = 26.2 bits (56), Expect = 9.4
Identities = 12/33 (36%), Positives = 17/33 (51%)
Frame = +1
Query: 183 WIDHVSMRNCRDGLIDAIMGSTAITISNSHFTD 215
WI++ S C GL+D TA+T N F +
Sbjct: 214 WINNGSTLGCGSGLVDYQCSRTALTSFNFSFLE 312
Database: LJGI
Posted date: Jul 30, 2004 11:16 AM
Number of letters in database: 14,692,800
Number of sequences in database: 28,460
Lambda K H
0.322 0.140 0.442
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 6,483,665
Number of Sequences: 28460
Number of extensions: 85153
Number of successful extensions: 451
Number of sequences better than 10.0: 32
Number of HSP's better than 10.0 without gapping: 446
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 447
length of query: 373
length of database: 4,897,600
effective HSP length: 92
effective length of query: 281
effective length of database: 2,279,280
effective search space: 640477680
effective search space used: 640477680
frameshift window, decay const: 50, 0.1
T: 13
A: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.9 bits)
S2: 56 (26.2 bits)
Medicago: description of AC136286.13